Restriction map for pF1KB8151
 (Linear) (Six-base) MAPSORT of: KIBB8151.seq  Check: 4352  from: 1  to: 912



KIBB8151.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AclI AA'CG_TT

Cuts at:      0     337     912 
   Size:        337     575
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     515     891     912 
   Size:        515     376      21

  Fragments arranged by size: 

                 515     376      21
 
AflIII A'CryG_T

Cuts at:      0     867     912 
   Size:        867      45
 
AlwNI CAG_nnn'CTG

Cuts at:      0     636     912 
   Size:        636     276
 
ApaI G_GGCC'C

Cuts at:      0     659     710     912 
   Size:        659      51     202

  Fragments arranged by size: 

                 659     202      51
 
ApoI r'AATT_y

Cuts at:      0     304     912 
   Size:        304     608
 
AseI AT'TA_AT

Cuts at:      0     806     912 
   Size:        806     106
 
AvaI C'yCGr_G

Cuts at:      0     117     130     238     912 
   Size:        117      13     108     674

  Fragments arranged by size: 

                 674     117     108      13
 
BanI G'GyrC_C

Cuts at:      0     210     912 
   Size:        210     702
 
BanII G_rGCy'C

Cuts at:      0     250     417     659     710     912 
   Size:        250     167     242      51     202

  Fragments arranged by size: 

                 250     242     202     167      51
 
BglII A'GATC_T

Cuts at:      0     447     912 
   Size:        447     465
 
BlpI GC'TnA_GC

Cuts at:      0     145     912 
   Size:        145     767
 
Bme1580I G_kGCm'C

Cuts at:      0     659     710     912 
   Size:        659      51     202

  Fragments arranged by size: 

                 659     202      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      51     646     912 
   Size:         51     595     266

  Fragments arranged by size: 

                 595     266      51
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     400     912 
   Size:        400     512
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     237     682     912 
   Size:        237     445     230

  Fragments arranged by size: 

                 445     237     230
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     495     912 
   Size:        495     417
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     388     418     912 
   Size:        388      30     494

  Fragments arranged by size: 

                 494     388      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      37     409     912 
   Size:         37     372     503

  Fragments arranged by size: 

                 503     372      37
 
BseYI C'CCAG_C

Cuts at:      0     227     670     912 
   Size:        227     443     242

  Fragments arranged by size: 

                 443     242     227
 
BsiHKAI G_wGCw'C

Cuts at:      0     124     146     912 
   Size:        124      22     766

  Fragments arranged by size: 

                 766     124      22
 
BsmI GAATG_Cn'

Cuts at:      0     516     912 
   Size:        516     396
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     156     174     912 
   Size:        156      18     738

  Fragments arranged by size: 

                 738     156      18
 
Bsp1286I G_dGCh'C

Cuts at:      0     124     146     250     417     659     710     912 
   Size:        124      22     104     167     242      51     202

  Fragments arranged by size: 

                 242     202     167     124     104      51      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     582     912 
   Size:        582     330
 
BsrBI CCG'CTC

Cuts at:      0      27     687     912 
   Size:         27     660     225

  Fragments arranged by size: 

                 660     225      27
 
BsrDI GCAATG_nn'

Cuts at:      0     286     912 
   Size:        286     626
 
BsrFI r'CCGG_y

Cuts at:      0     207     912 
   Size:        207     705
 
BstEII G'GTnAC_C

Cuts at:      0     607     912 
   Size:        607     305
 
BstYI r'GATC_y

Cuts at:      0     358     447     912 
   Size:        358      89     465

  Fragments arranged by size: 

                 465     358      89
 
BtsI GCAGTG_nn'

Cuts at:      0     508     912 
   Size:        508     404
 
DraIII CAC_nnn'GTG

Cuts at:      0      99     640     912 
   Size:         99     541     272

  Fragments arranged by size: 

                 541     272      99
 
EarI CTCTTCn'nnn_

Cuts at:      0     491     912 
   Size:        491     421
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     224     415     570     912 
   Size:        224     191     155     342

  Fragments arranged by size: 

                 342     224     191     155
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     237     515     682     891     912 
   Size:        237     278     167     209      21

  Fragments arranged by size: 

                 278     237     209     167      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     656     912 
   Size:        656     256
 
EcoRI G'AATT_C

Cuts at:      0     304     912 
   Size:        304     608
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     780     912 
   Size:        780     132
 
HaeII r_GCGC'y

Cuts at:      0      76     912 
   Size:         76     836
 
HincII GTy'rAC

Cuts at:      0     678     912 
   Size:        678     234
 
MspA1I CmG'CkG

Cuts at:      0      90     194     912 
   Size:         90     104     718

  Fragments arranged by size: 

                 718     104      90
 
NspI r_CATG'y

Cuts at:      0     871     912 
   Size:        871      41
 
PciI A'CATG_T

Cuts at:      0     867     912 
   Size:        867      45
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     640     912 
   Size:        640     272
 
PfoI T'CCnGG_A

Cuts at:      0     482     912 
   Size:        482     430
 
PspOMI G'GGCC_C

Cuts at:      0     655     706     912 
   Size:        655      51     206

  Fragments arranged by size: 

                 655     206      51
 
PspXI vC'TCGA_Gb

Cuts at:      0     117     912 
   Size:        117     795
 
PstI C_TGCA'G

Cuts at:      0      83     912 
   Size:         83     829
 
SapI GCTCTTCn'nnn_

Cuts at:      0     491     912 
   Size:        491     421
 
SfcI C'TryA_G

Cuts at:      0      79     912 
   Size:         79     833
 
SgrAI Cr'CCGG_yG

Cuts at:      0     207     912 
   Size:        207     705
 
SmaI CCC'GGG

Cuts at:      0     132     240     912 
   Size:        132     108     672

  Fragments arranged by size: 

                 672     132     108
 
SmlI C'TyrA_G

Cuts at:      0     117     474     912 
   Size:        117     357     438

  Fragments arranged by size: 

                 438     357     117
 
StyI C'CwwG_G

Cuts at:      0     764     912 
   Size:        764     148
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     628     641     912 
   Size:        628      13     271

  Fragments arranged by size: 

                 628     271      13
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     817     849     912 
   Size:        817      32      63

  Fragments arranged by size: 

                 817      63      32
 
XhoI C'TCGA_G

Cuts at:      0     117     912 
   Size:        117     795
 
XmaI C'CCGG_G

Cuts at:      0     130     238     912 
   Size:        130     108     674

  Fragments arranged by size: 

                 674     130     108
 
XmnI GAAnn'nnTTC

Cuts at:      0     449     912 
   Size:        449     463
 

 Enzymes that do cut:

     AclI     AcuI   AflIII    AlwNI     ApaI     ApoI     AseI     AvaI
     BanI    BanII    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI   BstEII    BstYI     BtsI   DraIII
     EarI     EciI  Eco57MI EcoO109I    EcoRI     FalI    HaeII   HincII
   MspA1I     NspI     PciI    PflMI     PfoI   PspOMI    PspXI     PstI
     SapI     SfcI    SgrAI     SmaI     SmlI     StyI    TaqII     TstI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI     BsgI    BsiEI    BsiWI    BspEI    BspHI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI  EcoICRI    EcoNI
    EcoRV     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI
     ZraI
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