Restriction map for pF1KE1041
 (Linear) (Six-base) MAPSORT of: KIEE1041.seq  Check: 1749  from: 1  to: 1050



KIEE1041.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73      91     302     452     814     947    1050 
   Size:         73      18     211     150     362     133     103

  Fragments arranged by size: 

                 362     211     150     133     103      73      18
 
AhdI GACnn_n'nnGTC

Cuts at:      0     750    1050 
   Size:        750     300
 
AleI CACnn'nnGTG

Cuts at:      0     815    1050 
   Size:        815     235
 
AlwNI CAG_nnn'CTG

Cuts at:      0     355    1050 
   Size:        355     695
 
ApaI G_GGCC'C

Cuts at:      0     313    1050 
   Size:        313     737
 
ApoI r'AATT_y

Cuts at:      0     760    1050 
   Size:        760     290
 
BanII G_rGCy'C

Cuts at:      0     313    1050 
   Size:        313     737
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     862    1050 
   Size:        862     188
 
Bme1580I G_kGCm'C

Cuts at:      0      79     313    1050 
   Size:         79     234     737

  Fragments arranged by size: 

                 737     234      79
 
BmgBI CAC'GTC

Cuts at:      0     601    1050 
   Size:        601     449
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     932     971    1050 
   Size:        458     474      39      79

  Fragments arranged by size: 

                 474     458      79      39
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     495    1050 
   Size:        495     555
 
BsaAI yAC'GTr

Cuts at:      0     728    1050 
   Size:        728     322
 
BseYI C'CCAG_C

Cuts at:      0     605     984    1050 
   Size:        605     379      66

  Fragments arranged by size: 

                 605     379      66
 
BsmI GAATG_Cn'

Cuts at:      0     508    1050 
   Size:        508     542
 
Bsp1286I G_dGCh'C

Cuts at:      0      79     313    1050 
   Size:         79     234     737

  Fragments arranged by size: 

                 737     234      79
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     189    1050 
   Size:        189     861
 
BsrDI GCAATG_nn'

Cuts at:      0     185     600     795    1050 
   Size:        185     415     195     255

  Fragments arranged by size: 

                 415     255     195     185
 
BsrGI T'GTAC_A

Cuts at:      0     549    1050 
   Size:        549     501
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     404    1050 
   Size:        404     646
 
BstBI TT'CG_AA

Cuts at:      0     585    1050 
   Size:        585     465
 
BstEII G'GTnAC_C

Cuts at:      0    1039    1050 
   Size:       1039      11
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     582     617    1050 
   Size:        582      35     433

  Fragments arranged by size: 

                 582     433      35
 
EaeI y'GGCC_r

Cuts at:      0      31    1050 
   Size:         31    1019
 
EarI CTCTTCn'nnn_

Cuts at:      0     911    1050 
   Size:        911     139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73      91     302     452     458     814     932     947 
   Size:         73      18     211     150       6     356     118      15

Cuts at:    947     971    1050 
   Size:         24      79

  Fragments arranged by size: 

                 356     211     150     118      79      73      24      18
                  15       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     309    1050 
   Size:        309     741
 
EcoRI G'AATT_C

Cuts at:      0     760    1050 
   Size:        760     290
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     656     688     736     768    1050 
   Size:        656      32      48      32     282

  Fragments arranged by size: 

                 656     282      48      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     642    1050 
   Size:        642     408
 
MscI TGG'CCA

Cuts at:      0      33    1050 
   Size:         33    1017
 
MslI CAynn'nnrTG

Cuts at:      0     815    1050 
   Size:        815     235
 
MspA1I CmG'CkG

Cuts at:      0     815    1050 
   Size:        815     235
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     355    1050 
   Size:        355     695
 
PfoI T'CCnGG_A

Cuts at:      0     354    1050 
   Size:        354     696
 
PsiI TTA'TAA

Cuts at:      0     455    1050 
   Size:        455     595
 
PspOMI G'GGCC_C

Cuts at:      0     309    1050 
   Size:        309     741
 
PstI C_TGCA'G

Cuts at:      0     233    1050 
   Size:        233     817
 
PvuII CAG'CTG

Cuts at:      0     815    1050 
   Size:        815     235
 
SapI GCTCTTCn'nnn_

Cuts at:      0     911    1050 
   Size:        911     139
 
SexAI A'CCwGG_T

Cuts at:      0     483    1050 
   Size:        483     567
 
SfcI C'TryA_G

Cuts at:      0     229    1050 
   Size:        229     821
 
SmlI C'TyrA_G

Cuts at:      0     510    1050 
   Size:        510     540
 
SspI AAT'ATT

Cuts at:      0       7    1050 
   Size:          7    1043
 
TatI w'GTAC_w

Cuts at:      0     194     549    1050 
   Size:        194     355     501

  Fragments arranged by size: 

                 501     355     194
 

 Enzymes that do cut:

     AcuI     AhdI     AleI    AlwNI     ApaI     ApoI    BanII     BbsI
 Bme1580I    BmgBI     BpmI    BpuEI    BsaAI    BseYI     BsmI Bsp1286I
    BspMI    BsrDI    BsrGI   BstAPI    BstBI   BstEII    CspCI     EaeI
     EarI  Eco57MI EcoO109I    EcoRI    Hin4I     MmeI     MscI     MslI
   MspA1I    PflMI     PfoI     PsiI   PspOMI     PstI    PvuII     SapI
    SexAI     SfcI     SmlI     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
   BssHII    BssSI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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