Restriction map for pF1KB6324
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BbeI        BbsI        
BbvCI       BcgI        BciVI       BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstUI       BstZ17I     
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO109I    EcoRI       EcoRV       EcoT22I     FauI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MroI        MslI        
MspA1I      MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AclI           67  AACGTT               AA^CGTT
AfaI           42  GTAC                 GT^AC
AflIII        121  ACGTGT               A^C[GA][CT]GT
AluI           96  AGCT                 AG^CT
AocI          415  CCTGAGG              CC^T[ACGT]AGG
BalI           53  TGGCCA               TGG^CCA
BanII         484  GGGCTC               G[GA]GC[CT]^C
BbrPI         120  CACGTG               CAC^GTG
BclI           36  TGATCA               T^GATCA
BmyI          484  GGGCTC               G[AGT]GC[ACT]^C
BsaAI         120  CACGTG               [CT]AC^GT[GA]
BsaI          421  GAGACC               GGTCTC(1/5)
BsgI          317  CTGCAC               GTGCAG(16/14)
BsmFI         307  GGGAC                GGGAC(10/14)
Bsp1286I      484  GGGCTC               G[AGT]GC[ACT]^C
BstEII        346  GGTTACC              G^GT[ACGT]ACC
BstF5I         33  GGATG                GGATG(2/0)
BstPI         346  GGTTACC              G^GT[ACGT]ACC
BstXI         336  CCAAGGTTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          20  AGATCC               [GA]^GATC[CT]
Bsu36I        415  CCTGAGG              CC^T[ACGT]AGG
DraI          217  TTTAAA               TTT^AAA
EaeI           53  TGGCCA               [CT]^GGCC[GA]
EarI          229  GAAGAG               CTCTTC(1/4)
Eco81I        415  CCTGAGG              CC^T[ACGT]AGG
EcoNI         298  CCTATGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       346  GGTTACC              G^GT[ACGT]ACC
EcoT14I       336  CCAAGG               C^C[AT][AT]GG
FbaI           36  TGATCA               T^GATCA
FokI           33  GGATG                GGATG(9/13)
HaeIII         54  GGCC                 GG^CC
HincII        658  GTCAAC               GT[CT]^[GA]AC
HindII        658  GTCAAC               GT[CT]^[GA]AC
HphI          384  GGTGA                GGTGA(8/7)
Ksp632I       229  GAAGAG               CTCTTC(1/4)
MflI           20  AGATCC               [GA]^GATC[CT]
MluNI          53  TGGCCA               TGG^CCA
MscI           53  TGGCCA               TGG^CCA
NspI          114  ACATGC               [GA]CATG^[CT]
PmaCI         120  CACGTG               CAC^GTG
PmlI          120  CACGTG               CAC^GTG
PshAI         651  GACCCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I       67  AACGTT               AA^CGTT
RsaI           42  GTAC                 GT^AC
SfaNI         357  GATGC                GCATC(5/9)
StyI          336  CCAAGG               C^C[AT][AT]GG
Tsp45I        385  GTGAC                ^GT[GC]AC
TspRI         155  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII          20  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AlwI           21  GATCC                GGATC(4/5)
              289  GGATC               
AseI          250  ATTAAT               AT^TAAT
              457  ATTAAT              
AsnI          250  ATTAAT               AT^TAAT
              457  ATTAAT              
Asp700I       631  GAAGCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              823  GAAGAAGTTC          
BbvI          443  GCAGC                GCAGC(8/12)
              812  GCTGC               
BseRI         377  GAGGAG               GAGGAG(10/8)
              418  GAGGAG              
BsiYI          24  CCAAGACGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              298  CCTATGCCAGG         
BslI           24  CCAAGACGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              298  CCTATGCCAGG         
BsmAI         421  GAGAC                GTCTC(1/5)
              543  GTCTC               
BstBI         245  TTCGAA               TT^CGAA
              323  TTCGAA              
Cac8I          55  GCCAGC               GC[ACGT]^[ACGT]GC
              177  GCAAGC              
DdeI          416  CTGAG                C^T[ACGT]AG
              839  CTGAG               
Fnu4HI        443  GCAGC                GC^[ACGT]GC
              812  GCTGC               
ItaI          443  GCAGC                GC^[ACGT]GC
              812  GCTGC               
MaeII          68  ACGT                 A^CGT
              121  ACGT                
NlaIII        115  CATG                 CATG^
              540  CATG                
NlaIV         411  GGAACC               GG[ACGT]^[ACGT]CC
              485  GGCTCC              
NspV          245  TTCGAA               TT^CGAA
              323  TTCGAA              
PleI          232  GAGTC                GAGTC(4/5)
              841  GAGTC               
PshBI         250  ATTAAT               AT^TAAT
              457  ATTAAT              
PstI          441  CTGCAG               CTGCA^G
              472  CTGCAG              
SfcI          441  CTGCAG               C^T[GA][CT]AG
              472  CTGCAG              
SfuI          245  TTCGAA               TT^CGAA
              323  TTCGAA              
SspI          204  AATATT               AAT^ATT
              803  AATATT              
TaqI          246  TCGA                 T^CGA
              324  TCGA                
TseI          443  GCAGC                G^C[AT]GC
              812  GCTGC               
TthHB8I       246  TCGA                 T^CGA
              324  TCGA                
XmnI          631  GAAGCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              823  GAAGAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

BsaJI       BssKI       BstNI       DpnII       EcoRII      
HinfI       MaeIII      MboI        MboII       MnlI        
MseI        MvaI        NdeII       Sau3AI      ScrFI       
TfiI        Tru9I       Tsp509I     
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