(Circular) (Six-base) MAPSORT of: pF1KB7770.seq Check: 2162 from: 1 to: 4544 pF1KB7770 4544 bp With 182 enzymes: SgfI * April 24, 2009 13:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1458 1458 Size: 4544 AccI GT'mk_AC Cuts at: 1582 1582 Size: 4544 Acc65I G'GTAC_C Cuts at: 1560 1560 Size: 4544 AclI AA'CG_TT Cuts at: 4384 4384 Size: 4544 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1390 2369 2801 3548 3816 1390 Size: 979 432 747 268 2118 Fragments arranged by size: 2118 979 747 432 268 AflIII A'CryG_T Cuts at: 1452 1896 3000 3977 1452 Size: 444 1104 977 2019 Fragments arranged by size: 2019 1104 977 444 AgeI A'CCGG_T Cuts at: 2932 2932 Size: 4544 AhdI GACnn_n'nnGTC Cuts at: 1441 1441 Size: 4544 AlwNI CAG_nnn'CTG Cuts at: 1464 1849 3416 1464 Size: 385 1567 2592 Fragments arranged by size: 2592 1567 385 ApaI G_GGCC'C Cuts at: 747 747 Size: 4544 ApaLI G'TGCA_C Cuts at: 3314 3314 Size: 4544 ApoI r'AATT_y Cuts at: 864 1548 3897 4153 864 Size: 684 2349 256 1255 Fragments arranged by size: 2349 1255 684 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4544 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4544 AvaI C'yCGr_G Cuts at: 1564 1564 Size: 4544 BamHI G'GATC_C Cuts at: 566 1569 566 Size: 1003 3541 BanI G'GyrC_C Cuts at: 1560 1974 2222 2257 1560 Size: 414 248 35 3847 Fragments arranged by size: 3847 414 248 35 BanII G_rGCy'C Cuts at: 747 1205 1364 1558 2588 747 Size: 458 159 194 1030 2703 Fragments arranged by size: 2703 1030 458 194 159 BbeI G_GCGC'C Cuts at: 1978 2226 1978 Size: 248 4296 BbsI GAAGACnn'nnnn_ Cuts at: 191 266 385 1334 1486 1492 191 Size: 75 119 949 152 6 3243 Fragments arranged by size: 3243 949 152 119 75 6 BciVI GTATCCnnnnn_n' Cuts at: 2442 3203 4544 2442 Size: 761 1341 2442 Fragments arranged by size: 2442 1341 761 BclI T'GATC_A Cuts at: 1472 2064 1472 Size: 592 3952 BglI GCCn_nnn'nGGC Cuts at: 138 138 Size: 4544 BglII A'GATC_T Cuts at: 695 1520 2059 4096 695 Size: 825 539 2037 1143 Fragments arranged by size: 2037 1143 825 539 BlpI GC'TnA_GC Cuts at: 1656 1656 Size: 4544 Bme1580I G_kGCm'C Cuts at: 747 2169 2262 3318 747 Size: 1422 93 1056 1973 Fragments arranged by size: 1973 1422 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1756 1837 2172 4316 1756 Size: 81 335 2144 1984 Fragments arranged by size: 2144 1984 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2911 2911 Size: 4544 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 219 684 1348 1378 1399 219 Size: 465 664 30 21 3364 Fragments arranged by size: 3364 664 465 30 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1720 2599 3091 3389 1720 Size: 879 492 298 2875 Fragments arranged by size: 2875 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1286 4248 1286 Size: 2962 1582 BsaAI yAC'GTr Cuts at: 1455 1897 1455 Size: 442 4102 BsaBI GATnn'nnATC Cuts at: 6 2058 4095 6 Size: 2052 2037 455 Fragments arranged by size: 2052 2037 455 BsaHI Gr'CG_yC Cuts at: 1975 2223 2925 4421 1975 Size: 248 702 1496 2098 Fragments arranged by size: 2098 1496 702 248 BsaWI w'CCGG_w Cuts at: 512 1733 2254 2932 3206 3353 3764 512 Size: 1221 521 678 274 147 411 1292 Fragments arranged by size: 1292 1221 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 181 211 670 700 1192 1222 181 Size: 30 459 30 492 30 3503 Fragments arranged by size: 3503 492 459 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1048 1048 Size: 4544 BseYI C'CCAG_C Cuts at: 1969 2727 3304 3800 1969 Size: 758 577 496 2713 Fragments arranged by size: 2713 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1006 1477 1006 Size: 471 4073 BsiEI CG_ry'CG Cuts at: 104 2086 2132 3340 104 Size: 1982 46 1208 1308 Fragments arranged by size: 1982 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1364 1558 2336 2526 3318 1364 Size: 194 778 190 792 2590 Fragments arranged by size: 2590 792 778 194 190 BsmI GAATG_Cn' Cuts at: 1013 1013 Size: 4544 Bsp1286I G_dGCh'C Cuts at: 747 1205 1364 1558 2169 2262 2336 2526 2588 Size: 458 159 194 611 93 74 190 62 Cuts at: 2588 3318 747 Size: 730 1973 Fragments arranged by size: 1973 730 611 458 194 190 159 93 74 62 BspEI T'CCGG_A Cuts at: 512 3764 512 Size: 3252 1292 BspHI T'CATG_A Cuts at: 402 1148 3720 402 Size: 746 2572 1226 Fragments arranged by size: 2572 1226 746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1352 1458 1594 1808 2110 2491 1352 Size: 106 136 214 302 381 3405 Fragments arranged by size: 3405 381 302 214 136 106 BsrBI CCG'CTC Cuts at: 50 1509 2836 2890 4374 4539 50 Size: 1459 1327 54 1484 165 55 Fragments arranged by size: 1484 1459 1327 165 55 54 BsrDI GCAATG_nn' Cuts at: 2456 2456 Size: 4544 BsrFI r'CCGG_y Cuts at: 2542 2723 2932 2542 Size: 181 209 4154 Fragments arranged by size: 4154 209 181 BssSI C'ACGA_G Cuts at: 1075 2815 3173 1075 Size: 1740 358 2446 Fragments arranged by size: 2446 1740 358 BstAPI GCAn_nnn'nTGC Cuts at: 1464 1921 1464 Size: 457 4087 BstBI TT'CG_AA Cuts at: 2905 2905 Size: 4544 BstXI CCAn_nnnn'nTGG Cuts at: 114 114 Size: 4544 BstYI r'GATC_y Cuts at: 1 168 282 566 695 1520 1569 2036 2059 Size: 167 114 284 129 825 49 467 23 Cuts at: 2059 2394 2640 3652 3738 3750 4096 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 825 467 449 346 335 284 246 167 129 114 86 49 23 12 BtgI C'CryG_G Cuts at: 113 164 974 113 Size: 51 810 3683 Fragments arranged by size: 3683 810 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2494 2675 2838 2494 Size: 181 163 4200 Fragments arranged by size: 4200 181 163 BtsI GCAGTG_nn' Cuts at: 629 1787 629 Size: 1158 3386 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 132 167 326 361 132 Size: 35 159 35 4315 Fragments arranged by size: 4315 159 35 35 DraI TTT'AAA Cuts at: 1543 1543 Size: 4544 DrdI GACnn_nn'nnGTC Cuts at: 2250 3108 2250 Size: 858 3686 EaeI y'GGCC_r Cuts at: 938 2129 2303 2694 2721 4066 938 Size: 1191 174 391 27 1345 1416 Fragments arranged by size: 1416 1345 1191 391 174 27 EagI C'GGCC_G Cuts at: 2129 2129 Size: 4544 EarI CTCTTCn'nnn_ Cuts at: 401 2567 2777 401 Size: 2166 210 2168 Fragments arranged by size: 2168 2166 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 672 706 2752 3062 3208 4354 672 Size: 34 2046 310 146 1146 862 Fragments arranged by size: 2046 1146 862 310 146 34 EcoICRI GAG'CTC Cuts at: 1362 1556 1362 Size: 194 4350 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 219 684 1348 1378 1390 1399 2369 2801 3548 Size: 465 664 30 12 9 970 432 747 Cuts at: 3548 3816 219 Size: 268 947 Fragments arranged by size: 970 947 747 664 465 432 268 30 12 9 EcoO109I rG'GnC_Cy Cuts at: 744 1683 744 Size: 939 3605 EcoRI G'AATT_C Cuts at: 864 1548 864 Size: 684 3860 EcoRV GAT'ATC Cuts at: 207 708 207 Size: 501 4043 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 525 2563 525 Size: 2038 2506 FspI TGC'GCA Cuts at: 2325 2325 Size: 4544 HaeII r_GCGC'y Cuts at: 1978 2226 3248 4227 1978 Size: 248 1022 979 2295 Fragments arranged by size: 2295 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 340 372 1427 1459 2386 2416 2418 2448 340 Size: 32 1055 32 927 30 2 30 2436 Fragments arranged by size: 2436 1055 927 32 32 30 30 2 HincII GTy'rAC Cuts at: 120 228 1583 120 Size: 108 1355 3081 Fragments arranged by size: 3081 1355 108 HindIII A'AGCT_T Cuts at: 458 532 458 Size: 74 4470 KasI G'GCGC_C Cuts at: 1974 2222 1974 Size: 248 4296 KpnI G_GTAC'C Cuts at: 1564 1564 Size: 4544 MfeI C'AATT_G Cuts at: 431 431 Size: 4544 MluI A'CGCG_T Cuts at: 3977 3977 Size: 4544 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 440 544 3215 3399 440 Size: 104 2671 184 1585 Fragments arranged by size: 2671 1585 184 104 MscI TGG'CCA Cuts at: 940 2305 940 Size: 1365 3179 MslI CAynn'nnrTG Cuts at: 112 127 488 761 2533 2660 112 Size: 15 361 273 1772 127 1996 Fragments arranged by size: 1996 1772 361 273 127 15 MspA1I CmG'CkG Cuts at: 717 733 789 1368 1398 1655 1969 2329 3342 Size: 16 56 579 30 257 314 360 1013 Cuts at: 3342 3587 717 Size: 245 1674 Fragments arranged by size: 1674 1013 579 360 314 257 245 56 30 16 NaeI GCC'GGC Cuts at: 2725 2725 Size: 4544 NarI GG'CG_CC Cuts at: 1975 2223 1975 Size: 248 4296 NcoI C'CATG_G Cuts at: 113 113 Size: 4544 NgoMIV G'CCGG_C Cuts at: 2723 2723 Size: 4544 NspI r_CATG'y Cuts at: 1597 2097 3004 1597 Size: 500 907 3137 Fragments arranged by size: 3137 907 500 PciI A'CATG_T Cuts at: 3000 3000 Size: 4544 PmeI GTTT'AAAC Cuts at: 1543 1543 Size: 4544 PmlI CAC'GTG Cuts at: 1455 1455 Size: 4544 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 3683 Size: 32 4512 PshAI GACnn'nnGTC Cuts at: 1496 1496 Size: 4544 PspOMI G'GGCC_C Cuts at: 743 743 Size: 4544 PstI C_TGCA'G Cuts at: 920 1469 1517 1591 2276 920 Size: 549 48 74 685 3188 Fragments arranged by size: 3188 685 549 74 48 PvuI CG_AT'CG Cuts at: 104 104 Size: 4544 PvuII CAG'CTG Cuts at: 717 1368 1398 1969 2329 717 Size: 651 30 571 360 2932 Fragments arranged by size: 2932 651 571 360 30 RsrII CG'GwC_CG Cuts at: 2739 2739 Size: 4544 SacI G_AGCT'C Cuts at: 1364 1558 1364 Size: 194 4350 SalI G'TCGA_C Cuts at: 1581 1581 Size: 4544 SapI GCTCTTCn'nnn_ Cuts at: 401 2567 2777 401 Size: 2166 210 2168 Fragments arranged by size: 2168 2166 210 SbfI CC_TGCA'GG Cuts at: 920 1517 1591 920 Size: 597 74 3873 Fragments arranged by size: 3873 597 74 ScaI AGT'ACT Cuts at: 598 4061 598 Size: 3463 1081 SfcI C'TryA_G Cuts at: 33 916 1465 1513 1587 2272 3265 3456 33 Size: 883 549 48 74 685 993 191 1121 Fragments arranged by size: 1121 993 883 685 549 191 74 48 SfoI GGC'GCC Cuts at: 1976 2224 1976 Size: 248 4296 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4544 SmaI CCC'GGG Cuts at: 1566 1566 Size: 4544 SmlI C'TyrA_G Cuts at: 1699 2614 3106 3368 1699 Size: 915 492 262 2875 Fragments arranged by size: 2875 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4544 SphI G_CATG'C Cuts at: 1597 2097 1597 Size: 500 4044 SspI AAT'ATT Cuts at: 1265 1265 Size: 4544 StyI C'CwwG_G Cuts at: 113 1140 1678 2032 113 Size: 1027 538 354 2625 Fragments arranged by size: 2625 1027 538 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2127 2260 2719 2928 2127 Size: 133 459 209 3743 Fragments arranged by size: 3743 459 209 133 TatI w'GTAC_w Cuts at: 596 4059 596 Size: 3463 1081 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 181 213 1372 1404 4061 4093 181 Size: 32 1159 32 2657 32 632 Fragments arranged by size: 2657 1159 632 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2341 2341 Size: 4544 XbaI T'CTAG_A Cuts at: 1575 1575 Size: 4544 XcmI CCAnnnn_n'nnnnTGG Cuts at: 423 2039 423 Size: 1616 2928 XmaI C'CCGG_G Cuts at: 1564 1564 Size: 4544 XmnI GAAnn'nnTTC Cuts at: 263 1331 263 Size: 1068 3476 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AleI AlfI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI HpaI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PpuMI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI