Restriction map for pF1KB3398
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AgeI        
AluI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspHI       AspI        AvaII       
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BglI        BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DrdI        DsaI        EagI        EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT14I     FauI        FbaI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MluI        
MroI        MslI        MspA1I      MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AfaI          187  GTAC                 GT^AC
AflII         223  CTTAAG               C^TTAAG
AhdI           56  GACGCAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI          56  GACGCAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI           19  CCCGGG               C^[CT]CG[GA]G
BalI          119  TGGCCA               TGG^CCA
BbvI           59  GCAGC                GCAGC(8/12)
BfrI          223  CTTAAG               C^TTAAG
BglII         258  AGATCT               A^GATCT
BsiYI          19  CCCGGGAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           19  CCCGGGAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI          19  CCCGGG               C^[CT]CG[GA]G
BstBI         293  TTCGAA               TT^CGAA
BstF5I        327  GGATG                GGATG(2/0)
BstNI         444  CCTGG                CC^[AT]GG
BstYI         258  AGATCT               [GA]^GATC[CT]
DraIII        170  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          119  TGGCCA               [CT]^GGCC[GA]
Eam1105I       56  GACGCAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         218  GAATTC               G^AATTC
EcoRII        444  CCTGG                ^CC[AT]GG
EcoT22I        10  ATGCAT               ATGCA^T
Fnu4HI         59  GCAGC                GC^[ACGT]GC
FokI          327  GGATG                GGATG(9/13)
HincII         81  GTTGAC               GT[CT]^[GA]AC
HindII         81  GTTGAC               GT[CT]^[GA]AC
HphI          366  TCACC                GGTGA(8/7)
ItaI           59  GCAGC                GC^[ACGT]GC
MaeIII        196  GTGAC                ^GT[ACGT]AC
MfeI          411  CAATTG               C^AATTG
MflI          258  AGATCT               [GA]^GATC[CT]
MluNI         119  TGGCCA               TGG^CCA
MscI          119  TGGCCA               TGG^CCA
MunI          411  CAATTG               C^AATTG
MvaI          444  CCTGG                CC^[AT]GG
MwoI          442  GCCCTGGTCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI           10  ATGCAT               ATGCA^T
NspV          293  TTCGAA               TT^CGAA
PacI          271  TTAATTAA             TTAAT^TAA
PleI          398  GAGTC                GAGTC(4/5)
RsaI          187  GTAC                 GT^AC
SfcI          431  CTACAG               C^T[GA][CT]AG
SfuI          293  TTCGAA               TT^CGAA
SmaI           19  CCCGGG               CCC^GGG
TseI           59  GCAGC                G^C[AT]GC
Tsp45I        196  GTGAC                ^GT[GC]AC
XhoII         258  AGATCT               [GA]^GATC[CT]
XmaCI          19  CCCGGG               C^CCGGG
XmaI           19  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI         284  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              434  CAGCCTCTG           
BcnI           19  CCCGG                CC^[GC]GG
               20  CCGGG               
BsaJI          19  CCCGGG               C^C[ACGT][ACGT]GG
              443  CCCTGG              
BsrI          117  ACTGG                ACTGG(1/-1)
               36  CCAGT               
Cac8I           8  GCATGC               GC[ACGT]^[ACGT]GC
               12  GCATGC              
Cfr13I         27  GGGCC                G^G[ACGT]CC
              129  GGGCC               
DdeI          160  CTGAG                C^T[ACGT]AG
              230  CTAAG               
DraII          26  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              128  AGGGCCT             
EcoO109I       26  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              128  AGGGCCT             
HapII          20  CCGG                 C^CGG
              360  CCGG                
HgaI           56  GACGC                GACGC(5/10)
               62  GCGTC               
HpaII          20  CCGG                 C^CGG
              360  CCGG                
MaeII          95  ACGT                 A^CGT
              189  ACGT                
MboII         256  GAAGA                GAAGA(8/7)
              249  TCTTC               
MspI           20  CCGG                 C^CGG
              360  CCGG                
NciI           19  CCCGG                CC^[GC]GG
               20  CCGGG               
NspI            8  GCATGC               [GA]CATG^[CT]
               12  GCATGC              
Sau96I         27  GGGCC                G^G[ACGT]CC
              129  GGGCC               
SfaNI         328  GATGC                GCATC(5/9)
              300  GCATC               
SmlI          223  CTTAAG               C^T[CT][GA]AG
              304  CTTGAG              
SphI            8  GCATGC               GCATG^C
               12  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AcsI        ApoI        BsmFI       BssKI       DpnII       
HaeIII      HinfI       MboI        MnlI        MseI        
NdeII       NlaIII      Sau3AI      ScrFI       TaqI        
TfiI        Tru9I       Tsp509I     TthHB8I     
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