Restriction map for pF1KB3398
 (Circular) (Six-base) MAPSORT of: pF1KB3398.seq  Check: 1659  from: 1  to: 3566



pF1KB3398  3566 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:    604     604 
   Size:       3566
 
Acc65I G'GTAC_C

Cuts at:    582     582 
   Size:       3566
 
AclI AA'CG_TT

Cuts at:   3406    3406 
   Size:       3566
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    383    1391    1823    2570    2838     383 
   Size:       1008     432     747     268    1111

  Fragments arranged by size: 

                1111    1008     747     432     268
 
AflII C'TTAA_G

Cuts at:    331     331 
   Size:       3566
 
AflIII A'CryG_T

Cuts at:    918    2022    2999     918 
   Size:       1104     977    1485

  Fragments arranged by size: 

                1485    1104     977
 
AgeI A'CCGG_T

Cuts at:   1954    1954 
   Size:       3566
 
AhdI GACnn_n'nnGTC

Cuts at:    169     169 
   Size:       3566
 
AlwNI CAG_nnn'CTG

Cuts at:    397     547     871    2438     397 
   Size:        150     324    1567    1525

  Fragments arranged by size: 

                1567    1525     324     150
 
ApaLI G'TGCA_C

Cuts at:   2336    2336 
   Size:       3566
 
ApoI r'AATT_y

Cuts at:    317     326     442     570    2919    3175     317 
   Size:          9     116     128    2349     256     708

  Fragments arranged by size: 

                2349     708     256     128     116       9
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3566
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3566
 
AvaI C'yCGr_G

Cuts at:    127     586     127 
   Size:        459    3107
 
BamHI G'GATC_C

Cuts at:    591     591 
   Size:       3566
 
BanI G'GyrC_C

Cuts at:    582     996    1244    1279     582 
   Size:        414     248      35    2869

  Fragments arranged by size: 

                2869     414     248      35
 
BanII G_rGCy'C

Cuts at:    580    1610     580 
   Size:       1030    2536
 
BbeI G_GCGC'C

Cuts at:   1000    1248    1000 
   Size:        248    3318
 
BciVI GTATCCnnnnn_n'

Cuts at:   1464    2225    3566    1464 
   Size:        761    1341    1464

  Fragments arranged by size: 

                1464    1341     761
 
BclI T'GATC_A

Cuts at:   1086    1086 
   Size:       3566
 
BfrBI ATG'CAT

Cuts at:    120     120 
   Size:       3566
 
BglII A'GATC_T

Cuts at:    366    1081    3118     366 
   Size:        715    2037     814

  Fragments arranged by size: 

                2037     814     715
 
BlpI GC'TnA_GC

Cuts at:    678     678 
   Size:       3566
 
Bme1580I G_kGCm'C

Cuts at:   1191    1284    2340    1191 
   Size:         93    1056    2417

  Fragments arranged by size: 

                2417    1056      93
 
BmgBI CAC'GTC

Cuts at:    204     204 
   Size:       3566
 
BmrI ACTGGGnnnn_n'

Cuts at:    138     778     859    1194    3338     138 
   Size:        640      81     335    2144     366

  Fragments arranged by size: 

                2144     640     366     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1933    1933 
   Size:       3566
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    433     742    1621    2113    2411     433 
   Size:        309     879     492     298    1588

  Fragments arranged by size: 

                1588     879     492     309     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3270    3270 
   Size:       3566
 
BsaAI yAC'GTr

Cuts at:    919     919 
   Size:       3566
 
BsaBI GATnn'nnATC

Cuts at:      6    1080    3117       6 
   Size:       1074    2037     455

  Fragments arranged by size: 

                2037    1074     455
 
BsaHI Gr'CG_yC

Cuts at:    997    1245    1947    3443     997 
   Size:        248     702    1496    1120

  Fragments arranged by size: 

                1496    1120     702     248
 
BsaWI w'CCGG_w

Cuts at:    755    1276    1954    2228    2375    2786     755 
   Size:        521     678     274     147     411    1535

  Fragments arranged by size: 

                1535     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    991    1749    2326    2822     991 
   Size:        758     577     496    1735

  Fragments arranged by size: 

                1735     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1108    1154    2362     104 
   Size:       1004      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1004      46
 
BsiHKAI G_wGCw'C

Cuts at:    580    1358    1548    2340     580 
   Size:        778     190     792    1806

  Fragments arranged by size: 

                1806     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    580    1191    1284    1358    1548    1610    2340     580 
   Size:        611      93      74     190      62     730    1806

  Fragments arranged by size: 

                1806     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2786    2786 
   Size:       3566
 
BspHI T'CATG_A

Cuts at:   2742    2742 
   Size:       3566
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    616     830    1132    1513     616 
   Size:        214     302     381    2669

  Fragments arranged by size: 

                2669     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1858    1912    3396    3561      50 
   Size:       1808      54    1484     165      55

  Fragments arranged by size: 

                1808    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1478    1478 
   Size:       3566
 
BsrFI r'CCGG_y

Cuts at:   1564    1745    1954    1564 
   Size:        181     209    3176

  Fragments arranged by size: 

                3176     209     181
 
BssSI C'ACGA_G

Cuts at:   1837    2195    1837 
   Size:        358    3208
 
BstAPI GCAn_nnn'nTGC

Cuts at:    943     943 
   Size:       3566
 
BstBI TT'CG_AA

Cuts at:    402    1927     402 
   Size:       1525    2041
 
BstYI r'GATC_y

Cuts at:      1     366     591    1058    1081    1416    1662    2674    2760 
   Size:        365     225     467      23     335     246    1012      86

Cuts at:   2760    2772    3118       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     365     346     335     246     225
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3566
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1516    1697    1860    1516 
   Size:        181     163    3222

  Fragments arranged by size: 

                3222     181     163
 
BtsI GCAGTG_nn'

Cuts at:    809     809 
   Size:       3566
 
DraI TTT'AAA

Cuts at:    565     565 
   Size:       3566
 
DraIII CAC_nnn'GTG

Cuts at:    283     283 
   Size:       3566
 
DrdI GACnn_nn'nnGTC

Cuts at:   1272    2130    1272 
   Size:        858    2708
 
EaeI y'GGCC_r

Cuts at:    227    1151    1325    1716    1743    3088     227 
   Size:        924     174     391      27    1345     705

  Fragments arranged by size: 

                1345     924     705     391     174      27
 
EagI C'GGCC_G

Cuts at:   1151    1151 
   Size:       3566
 
EarI CTCTTCn'nnn_

Cuts at:   1589    1799    1589 
   Size:        210    3356
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1774    2084    2230    3376    1774 
   Size:        310     146    1146    1964

  Fragments arranged by size: 

                1964    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    578     578 
   Size:       3566
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    383    1391    1823    2570    2838     383 
   Size:       1008     432     747     268    1111

  Fragments arranged by size: 

                1111    1008     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    135     237     705     135 
   Size:        102     468    2996

  Fragments arranged by size: 

                2996     468     102
 
EcoRI G'AATT_C

Cuts at:    326     570     326 
   Size:        244    3322
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1585    1585 
   Size:       3566
 
FspI TGC'GCA

Cuts at:   1347    1347 
   Size:       3566
 
HaeII r_GCGC'y

Cuts at:   1000    1248    2270    3249    1000 
   Size:        248    1022     979    1317

  Fragments arranged by size: 

                1317    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    155     187    1408    1438    1440    1470     155 
   Size:         32    1221      30       2      30    2251

  Fragments arranged by size: 

                2251    1221      32      30      30       2
 
HincII GTy'rAC

Cuts at:    191     605     191 
   Size:        414    3152
 
KasI G'GCGC_C

Cuts at:    996    1244     996 
   Size:        248    3318
 
KpnI G_GTAC'C

Cuts at:    586     586 
   Size:       3566
 
MfeI C'AATT_G

Cuts at:    519     519 
   Size:       3566
 
MluI A'CGCG_T

Cuts at:   2999    2999 
   Size:       3566
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2237    2421    2237 
   Size:        184    3382
 
MscI TGG'CCA

Cuts at:    229    1327     229 
   Size:       1098    2468
 
MslI CAynn'nnrTG

Cuts at:   1555    1682    1555 
   Size:        127    3439
 
MspA1I CmG'CkG

Cuts at:    677     991    1351    2364    2609     677 
   Size:        314     360    1013     245    1634

  Fragments arranged by size: 

                1634    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1747    1747 
   Size:       3566
 
NarI GG'CG_CC

Cuts at:    997    1245     997 
   Size:        248    3318
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3566
 
NgoMIV G'CCGG_C

Cuts at:   1745    1745 
   Size:       3566
 
NsiI A_TGCA'T

Cuts at:    122     122 
   Size:       3566
 
NspI r_CATG'y

Cuts at:    120     124     619    1119    2026     120 
   Size:          4     495     500     907    1660

  Fragments arranged by size: 

                1660     907     500     495       4
 
PacI TTA_AT'TAA

Cuts at:    383     383 
   Size:       3566
 
PciI A'CATG_T

Cuts at:   2022    2022 
   Size:       3566
 
PmeI GTTT'AAAC

Cuts at:    565     565 
   Size:       3566
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2705    2737    2705 
   Size:         32    3534
 
PsiI TTA'TAA

Cuts at:    494     494 
   Size:       3566
 
PstI C_TGCA'G

Cuts at:    613    1298     613 
   Size:        685    2881
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3566
 
PvuII CAG'CTG

Cuts at:    991    1351     991 
   Size:        360    3206
 
RsrII CG'GwC_CG

Cuts at:   1761    1761 
   Size:       3566
 
SacI G_AGCT'C

Cuts at:    580     580 
   Size:       3566
 
SalI G'TCGA_C

Cuts at:    603     603 
   Size:       3566
 
SapI GCTCTTCn'nnn_

Cuts at:   1589    1799    1589 
   Size:        210    3356
 
SbfI CC_TGCA'GG

Cuts at:    613     613 
   Size:       3566
 
ScaI AGT'ACT

Cuts at:   3083    3083 
   Size:       3566
 
SfcI C'TryA_G

Cuts at:     33     539     609    1294    2287    2478      33 
   Size:        506      70     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     506     191      70
 
SfoI GGC'GCC

Cuts at:    998    1246     998 
   Size:        248    3318
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3566
 
SmaI CCC'GGG

Cuts at:    129     588     129 
   Size:        459    3107
 
SmlI C'TyrA_G

Cuts at:    331     412     721    1636    2128    2390     331 
   Size:         81     309     915     492     262    1507

  Fragments arranged by size: 

                1507     915     492     309     262      81
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3566
 
SphI G_CATG'C

Cuts at:    120     124     619    1119     120 
   Size:          4     495     500    2567

  Fragments arranged by size: 

                2567     500     495       4
 
StyI C'CwwG_G

Cuts at:    107     700    1054     107 
   Size:        593     354    2619

  Fragments arranged by size: 

                2619     593     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1149    1282    1741    1950    1149 
   Size:        133     459     209    2765

  Fragments arranged by size: 

                2765     459     209     133
 
TatI w'GTAC_w

Cuts at:   3081    3081 
   Size:       3566
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3083    3115    3083 
   Size:         32    3534
 
Tth111I GACn'n_nGTC

Cuts at:   1363    1363 
   Size:       3566
 
XbaI T'CTAG_A

Cuts at:    597     597 
   Size:       3566
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1061    1061 
   Size:       3566
 
XmaI C'CCGG_G

Cuts at:    127     586     127 
   Size:        459    3107
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AhdI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI
     PacI     PciI     PmeI     PpiI     PsiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI     BglI     BmtI     BpmI
   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     NdeI     NheI     NotI
     NruI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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