(Circular) (Six-base) MAPSORT of: pF1KE1497.seq Check: 3792 from: 1 to: 5966 pF1KE1497 5966 bp With 182 enzymes: SgfI * June 22, 2010 14:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1903 2205 1903 Size: 302 5664 AccI GT'mk_AC Cuts at: 452 2939 3004 452 Size: 2487 65 3414 Fragments arranged by size: 3414 2487 65 Acc65I G'GTAC_C Cuts at: 2982 2982 Size: 5966 AclI AA'CG_TT Cuts at: 2960 5806 2960 Size: 2846 3120 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 970 2580 2683 2842 2917 2933 3791 4223 4970 Size: 1610 103 159 75 16 858 432 747 Cuts at: 4970 5238 970 Size: 268 1698 Fragments arranged by size: 1698 1610 858 747 432 268 159 103 75 16 AfeI AGC'GCT Cuts at: 1276 1276 Size: 5966 AflIII A'CryG_T Cuts at: 3318 4422 5399 3318 Size: 1104 977 3885 Fragments arranged by size: 3885 1104 977 AgeI A'CCGG_T Cuts at: 4354 4354 Size: 5966 AhdI GACnn_n'nnGTC Cuts at: 783 783 Size: 5966 AleI CACnn'nnGTG Cuts at: 1543 2898 1543 Size: 1355 4611 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 552 1561 552 Size: 1009 4957 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2846 2878 2846 Size: 32 5934 AlwNI CAG_nnn'CTG Cuts at: 1170 3271 4838 1170 Size: 2101 1567 2298 Fragments arranged by size: 2298 2101 1567 ApaI G_GGCC'C Cuts at: 2527 2527 Size: 5966 ApaLI G'TGCA_C Cuts at: 4736 4736 Size: 5966 ApoI r'AATT_y Cuts at: 1868 2970 5319 5575 1868 Size: 1102 2349 256 2259 Fragments arranged by size: 2349 2259 1102 256 AseI AT'TA_AT Cuts at: 20 719 20 Size: 699 5267 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5966 AvaI C'yCGr_G Cuts at: 1439 2986 1439 Size: 1547 4419 AvrII C'CTAG_G Cuts at: 2073 2073 Size: 5966 BamHI G'GATC_C Cuts at: 507 2991 507 Size: 2484 3482 BanI G'GyrC_C Cuts at: 1688 2256 2982 3396 3644 3679 1688 Size: 568 726 414 248 35 3975 Fragments arranged by size: 3975 726 568 414 248 35 BanII G_rGCy'C Cuts at: 2527 2910 2980 4010 2527 Size: 383 70 1030 4483 Fragments arranged by size: 4483 1030 383 70 BbeI G_GCGC'C Cuts at: 1692 3400 3648 1692 Size: 1708 248 4010 Fragments arranged by size: 4010 1708 248 BbsI GAAGACnn'nnnn_ Cuts at: 1068 2580 2649 1068 Size: 1512 69 4385 Fragments arranged by size: 4385 1512 69 BciVI GTATCCnnnnn_n' Cuts at: 2872 3864 4625 5966 2872 Size: 992 761 1341 2872 Fragments arranged by size: 2872 1341 992 761 BclI T'GATC_A Cuts at: 3486 3486 Size: 5966 BglI GCCn_nnn'nGGC Cuts at: 364 652 1928 364 Size: 288 1276 4402 Fragments arranged by size: 4402 1276 288 BglII A'GATC_T Cuts at: 3481 5518 3481 Size: 2037 3929 BlpI GC'TnA_GC Cuts at: 3078 3078 Size: 5966 Bme1580I G_kGCm'C Cuts at: 2354 2527 3591 3684 4740 2354 Size: 173 1064 93 1056 3580 Fragments arranged by size: 3580 1064 1056 173 93 BmrI ACTGGGnnnn_n' Cuts at: 2017 2521 3178 3259 3594 5738 2017 Size: 504 657 81 335 2144 2245 Fragments arranged by size: 2245 2144 657 504 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4333 4333 Size: 5966 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1537 2542 1537 Size: 1005 4961 Bpu10I CC'TnA_GC Cuts at: 1163 1163 Size: 5966 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1133 1570 1647 1961 3142 4021 4513 4811 1133 Size: 437 77 314 1181 879 492 298 2288 Fragments arranged by size: 2288 1181 879 492 437 314 298 77 BsaI GGTCTCn'nnnn_ Cuts at: 1661 5670 1661 Size: 4009 1957 BsaAI yAC'GTr Cuts at: 2860 3319 2860 Size: 459 5507 BsaBI GATnn'nnATC Cuts at: 6 401 506 623 769 3480 5517 6 Size: 395 105 117 146 2711 2037 455 Fragments arranged by size: 2711 2037 455 395 146 117 105 BsaHI Gr'CG_yC Cuts at: 1689 3397 3645 4347 5843 1689 Size: 1708 248 702 1496 1812 Fragments arranged by size: 1812 1708 1496 702 248 BsaWI w'CCGG_w Cuts at: 2326 3155 3676 4354 4628 4775 5186 2326 Size: 829 521 678 274 147 411 3106 Fragments arranged by size: 3106 829 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2166 2196 2166 Size: 30 5936 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1771 2093 2444 1771 Size: 322 351 5293 Fragments arranged by size: 5293 351 322 BseYI C'CCAG_C Cuts at: 2446 3391 4149 4726 5222 2446 Size: 945 758 577 496 3190 Fragments arranged by size: 3190 945 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1021 1567 1021 Size: 546 5420 BsiEI CG_ry'CG Cuts at: 104 3508 3554 4762 104 Size: 3404 46 1208 1308 Fragments arranged by size: 3404 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1906 2910 2980 3758 3948 4740 1906 Size: 1004 70 778 190 792 3132 Fragments arranged by size: 3132 1004 792 778 190 70 BsmI GAATG_Cn' Cuts at: 751 751 Size: 5966 BsmBI CGTCTCn'nnnn_ Cuts at: 1753 1753 Size: 5966 Bsp1286I G_dGCh'C Cuts at: 1906 2354 2527 2910 2980 3591 3684 3758 3948 Size: 448 173 383 70 611 93 74 190 Cuts at: 3948 4010 4740 1906 Size: 62 730 3132 Fragments arranged by size: 3132 730 611 448 383 190 173 93 74 70 62 BspEI T'CCGG_A Cuts at: 2326 5186 2326 Size: 2860 3106 BspHI T'CATG_A Cuts at: 1130 2531 5142 1130 Size: 1401 2611 1954 Fragments arranged by size: 2611 1954 1401 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1611 1903 2205 2304 3016 3230 3532 3913 1611 Size: 292 302 99 712 214 302 381 3664 Fragments arranged by size: 3664 712 381 302 302 292 214 99 BsrBI CCG'CTC Cuts at: 50 483 834 4258 4312 5796 5961 50 Size: 433 351 3424 54 1484 165 55 Fragments arranged by size: 3424 1484 433 351 165 55 54 BsrDI GCAATG_nn' Cuts at: 1157 1288 3878 1157 Size: 131 2590 3245 Fragments arranged by size: 3245 2590 131 BsrFI r'CCGG_y Cuts at: 3964 4145 4354 3964 Size: 181 209 5576 Fragments arranged by size: 5576 209 181 BssSI C'ACGA_G Cuts at: 4237 4595 4237 Size: 358 5608 BstAPI GCAn_nnn'nTGC Cuts at: 2179 2805 3343 2179 Size: 626 538 4802 Fragments arranged by size: 4802 626 538 BstBI TT'CG_AA Cuts at: 1218 4327 1218 Size: 3109 2857 BstXI CCAn_nnnn'nTGG Cuts at: 1257 2055 1257 Size: 798 5168 BstYI r'GATC_y Cuts at: 1 396 507 606 1995 2067 2991 3458 3481 Size: 395 111 99 1389 72 924 467 23 Cuts at: 3481 3816 4062 5074 5160 5172 5518 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1389 1012 924 467 449 395 346 335 246 111 99 86 72 23 12 Bsu36I CC'TnA_GG Cuts at: 920 1015 1961 2279 920 Size: 95 946 318 4607 Fragments arranged by size: 4607 946 318 95 BtgI C'CryG_G Cuts at: 233 434 233 Size: 201 5765 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1377 3916 4097 4260 1377 Size: 2539 181 163 3083 Fragments arranged by size: 3083 2539 181 163 BtsI GCAGTG_nn' Cuts at: 875 1937 2069 2109 2144 2217 3209 875 Size: 1062 132 40 35 73 992 3632 Fragments arranged by size: 3632 1062 992 132 73 40 35 ClaI AT'CG_AT Cuts at: 2582 2582 Size: 5966 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 617 652 617 Size: 35 5931 DraI TTT'AAA Cuts at: 2003 2726 2965 2003 Size: 723 239 5004 Fragments arranged by size: 5004 723 239 DraIII CAC_nnn'GTG Cuts at: 1403 2263 1403 Size: 860 5106 DrdI GACnn_nn'nnGTC Cuts at: 2090 3672 4530 2090 Size: 1582 858 3526 Fragments arranged by size: 3526 1582 858 EaeI y'GGCC_r Cuts at: 356 2459 2849 3551 3725 4116 4143 5488 356 Size: 2103 390 702 174 391 27 1345 834 Fragments arranged by size: 2103 1345 834 702 391 390 174 27 EagI C'GGCC_G Cuts at: 3551 3551 Size: 5966 EarI CTCTTCn'nnn_ Cuts at: 3989 4199 3989 Size: 210 5756 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4174 4484 4630 5776 4174 Size: 310 146 1146 4364 Fragments arranged by size: 4364 1146 310 146 EcoICRI GAG'CTC Cuts at: 2908 2978 2908 Size: 70 5896 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 970 1537 2542 2580 2683 2842 2917 2933 3791 Size: 567 1005 38 103 159 75 16 858 Cuts at: 3791 4223 4970 5238 970 Size: 432 747 268 1698 Fragments arranged by size: 1698 1005 858 747 567 432 268 159 103 75 38 16 EcoO109I rG'GnC_Cy Cuts at: 3105 3105 Size: 5966 EcoRI G'AATT_C Cuts at: 1868 2970 1868 Size: 1102 4864 EcoRV GAT'ATC Cuts at: 558 558 Size: 5966 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2002 3985 2002 Size: 1983 3983 FspI TGC'GCA Cuts at: 3747 3747 Size: 5966 HaeII r_GCGC'y Cuts at: 1278 1692 2652 3400 3648 4670 5649 1278 Size: 414 960 748 248 1022 979 1595 Fragments arranged by size: 1595 1022 979 960 748 414 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 276 308 769 801 2529 2561 3808 3838 3840 Size: 32 461 32 1728 32 1247 30 2 Cuts at: 3840 3870 276 Size: 30 2372 Fragments arranged by size: 2372 1728 1247 461 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1209 3005 1209 Size: 1796 4170 HindIII A'AGCT_T Cuts at: 307 1509 307 Size: 1202 4764 KasI G'GCGC_C Cuts at: 1688 3396 3644 1688 Size: 1708 248 4010 Fragments arranged by size: 4010 1708 248 KpnI G_GTAC'C Cuts at: 2986 2986 Size: 5966 MfeI C'AATT_G Cuts at: 1337 1337 Size: 5966 MluI A'CGCG_T Cuts at: 5399 5399 Size: 5966 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1103 4637 4821 1103 Size: 3534 184 2248 Fragments arranged by size: 3534 2248 184 MscI TGG'CCA Cuts at: 358 2461 3727 358 Size: 2103 1266 2597 Fragments arranged by size: 2597 2103 1266 MslI CAynn'nnrTG Cuts at: 1255 1543 2053 2359 2536 2726 2898 3955 4082 Size: 288 510 306 177 190 172 1057 127 Cuts at: 4082 1255 Size: 3139 Fragments arranged by size: 3139 1057 510 306 288 190 177 172 127 MspA1I CmG'CkG Cuts at: 883 2600 3077 3391 3751 4764 5009 883 Size: 1717 477 314 360 1013 245 1840 Fragments arranged by size: 1840 1717 1013 477 360 314 245 NaeI GCC'GGC Cuts at: 4147 4147 Size: 5966 NarI GG'CG_CC Cuts at: 1689 3397 3645 1689 Size: 1708 248 4010 Fragments arranged by size: 4010 1708 248 NcoI C'CATG_G Cuts at: 434 434 Size: 5966 NgoMIV G'CCGG_C Cuts at: 4145 4145 Size: 5966 NspI r_CATG'y Cuts at: 2808 3019 3519 4426 2808 Size: 211 500 907 4348 Fragments arranged by size: 4348 907 500 211 PciI A'CATG_T Cuts at: 4422 4422 Size: 5966 PflMI CCAn_nnn'nTGG Cuts at: 573 573 Size: 5966 PfoI T'CCnGG_A Cuts at: 773 1514 773 Size: 741 5225 PmeI GTTT'AAAC Cuts at: 2965 2965 Size: 5966 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1402 1434 2322 2354 5105 5137 1402 Size: 32 888 32 2751 32 2231 Fragments arranged by size: 2751 2231 888 32 32 32 PshAI GACnn'nnGTC Cuts at: 2015 2015 Size: 5966 PspOMI G'GGCC_C Cuts at: 2523 2523 Size: 5966 PspXI vC'TCGA_Gb Cuts at: 1439 1439 Size: 5966 PstI C_TGCA'G Cuts at: 1272 2301 2393 3013 3698 1272 Size: 1029 92 620 685 3540 Fragments arranged by size: 3540 1029 685 620 92 PvuI CG_AT'CG Cuts at: 104 104 Size: 5966 PvuII CAG'CTG Cuts at: 2600 3391 3751 2600 Size: 791 360 4815 Fragments arranged by size: 4815 791 360 RsrII CG'GwC_CG Cuts at: 4161 4161 Size: 5966 SacI G_AGCT'C Cuts at: 2910 2980 2910 Size: 70 5896 SalI G'TCGA_C Cuts at: 3003 3003 Size: 5966 SapI GCTCTTCn'nnn_ Cuts at: 3989 4199 3989 Size: 210 5756 SbfI CC_TGCA'GG Cuts at: 3013 3013 Size: 5966 ScaI AGT'ACT Cuts at: 5483 5483 Size: 5966 SexAI A'CCwGG_T Cuts at: 2259 2259 Size: 5966 SfcI C'TryA_G Cuts at: 33 1193 1268 2297 2389 3009 3694 4687 4878 Size: 1160 75 1029 92 620 685 993 191 Cuts at: 4878 33 Size: 1121 Fragments arranged by size: 1160 1121 1029 993 685 620 191 92 75 SfoI GGC'GCC Cuts at: 1690 3398 3646 1690 Size: 1708 248 4010 Fragments arranged by size: 4010 1708 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5966 SmaI CCC'GGG Cuts at: 2988 2988 Size: 5966 SmlI C'TyrA_G Cuts at: 1148 1439 1585 1626 1976 3121 4036 4528 4790 Size: 291 146 41 350 1145 915 492 262 Cuts at: 4790 1148 Size: 2324 Fragments arranged by size: 2324 1145 915 492 350 291 262 146 41 SnaBI TAC'GTA Cuts at: 2860 2860 Size: 5966 SpeI A'CTAG_T Cuts at: 67 67 Size: 5966 SphI G_CATG'C Cuts at: 3019 3519 3019 Size: 500 5466 SspI AAT'ATT Cuts at: 152 152 Size: 5966 StuI AGG'CCT Cuts at: 1047 1047 Size: 5966 StyI C'CwwG_G Cuts at: 419 434 2073 2109 2346 2519 3100 3454 419 Size: 15 1639 36 237 173 581 354 2931 Fragments arranged by size: 2931 1639 581 354 237 173 36 15 SwaI ATTT'AAAT Cuts at: 2726 2726 Size: 5966 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 695 1555 2005 2154 3549 3682 4141 4350 695 Size: 860 450 149 1395 133 459 209 2311 Fragments arranged by size: 2311 1395 860 459 450 209 149 133 TatI w'GTAC_w Cuts at: 1088 5481 1088 Size: 4393 1573 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5483 5515 5483 Size: 32 5934 Tth111I GACn'n_nGTC Cuts at: 3763 3763 Size: 5966 XbaI T'CTAG_A Cuts at: 906 2997 906 Size: 2091 3875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 867 1848 2454 2787 3461 867 Size: 981 606 333 674 3372 Fragments arranged by size: 3372 981 674 606 333 XhoI C'TCGA_G Cuts at: 1439 1439 Size: 5966 XmaI C'CCGG_G Cuts at: 2986 2986 Size: 5966 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AflII AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I EcoNI FseI FspAI HpaI NdeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PsiI PsrI SacII SanDI SfiI SgrAI SrfI XmnI ZraI