Restriction map for pF1KB9974
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AflIII      AhdI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BalI        BbrPI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsiWI       BspDI       
BspHI       BspLU11I    BspMI       BssHII      Bst1107I    
BstAPI      BstBI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HpaI        
KpnI        MaeI        MamI        MfeI        MluI        
MluNI       MscI        MseI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SbfI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Tru9I       Tsp509I     XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AatII        1987  GACGTC               GACGT^C
AccIII       1850  TCCGGA               T^CCGGA
AlwNI          13  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2113  CCTGAGG              CC^T[ACGT]AGG
BbvCI        1466  CCTCAGC              CCTCAGC(-5/-2)
BglI         1822  GCCGACGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        2086  TACGTG               [CT]AC^GT[GA]
BseAI        1850  TCCGGA               T^CCGGA
BseRI        1714  GAGGAG               GAGGAG(10/8)
BsmI          590  GCATTC               GAATGC(1/-1)
BspEI        1850  TCCGGA               T^CCGGA
BsrDI         842  GCAATG               GCAATG(2/0)
BssSI        1189  CTCGTG               CACGAG(-5/-1)
BstEII       1836  GGTGACC              G^GT[ACGT]ACC
BstPI        1836  GGTGACC              G^GT[ACGT]ACC
BstXI        1940  CCAGCGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2113  CCTGAGG              CC^T[ACGT]AGG
EagI         1645  CGGCCG               C^GGCCG
EclXI        1645  CGGCCG               C^GGCCG
Eco52I       1645  CGGCCG               C^GGCCG
Eco81I       2113  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1836  GGTGACC              G^GT[ACGT]ACC
HindIII      1732  AAGCTT               A^AGCTT
MroI         1850  TCCGGA               T^CCGGA
NaeI         1643  GCCGGC               GCC^GGC
NgoMIV       1643  GCCGGC               G^CCGGC
PflMI        1212  CCACGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI         2104  CTGCAG               CTGCA^G
SexAI        2066  ACCAGGT              A^CC[AT]GGT
SfcI         2104  CTGCAG               C^T[GA][CT]AG
SmlI          340  CTCAAG               C^T[CT][GA]AG
Van91I       1212  CCACGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AgeI          247  ACCGGT               A^CCGGT
              797  ACCGGT              
Asp700I      1554  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1731  GAAGCTTTTC          
AspI          493  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1702  GACTCGGTC           
BamHI         371  GGATCC               G^GATCC
             1926  GGATCC              
BcgI          414  CGACGCCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              638  GCAAGGGCGTCG        
BciVI        1174  GGATAC               GTATCC(6/5)
              534  GTATCC              
BsaI           98  GAGACC               GGTCTC(1/5)
              244  GAGACC              
BsmBI         527  GAGACG               CGTCTC(1/5)
              599  GAGACG              
Bsp1407I      476  TGTACA               T^GTACA
             1766  TGTACA              
BsrGI         476  TGTACA               T^GTACA
             1766  TGTACA              
BstYI         371  GGATCC               [GA]^GATC[CT]
             1926  GGATCC              
EarI           88  CTCTTC               CTCTTC(1/4)
              973  CTCTTC              
EcoNI         633  CCTGGGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1059  CCTGGGTAAGG         
EcoT14I       882  CCAAGG               C^C[AT][AT]GG
             1170  CCAAGG              
Ksp632I        88  CTCTTC               CTCTTC(1/4)
              973  CTCTTC              
KspI          397  CCGCGG               CCGC^GG
             1095  CCGCGG              
MflI          371  GGATCC               [GA]^GATC[CT]
             1926  GGATCC              
PflFI         493  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1702  GACTCGGTC           
PinAI         247  ACCGGT               A^CCGGT
              797  ACCGGT              
SacII         397  CCGCGG               CCGC^GG
             1095  CCGCGG              
SapI           87  GCTCTTC              GCTCTTC(1/4)
              972  GCTCTTC             
ScaI         1070  AGTACT               AGT^ACT
             1613  AGTACT              
SmaI          833  CCCGGG               CCC^GGG
             1963  CCCGGG              
SspBI         476  TGTACA               T^GTACA
             1766  TGTACA              
StyI          882  CCAAGG               C^C[AT][AT]GG
             1170  CCAAGG              
TfiI          317  GATTC                G^A[AT]TC
             1622  GATTC               
Tth111I       493  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1702  GACTCGGTC           
XhoII         371  GGATCC               [GA]^GATC[CT]
             1926  GGATCC              
XmaCI         833  CCCGGG               C^CCGGG
             1963  CCCGGG              
XmaI          833  CCCGGG               C^CCGGG
             1963  CCCGGG              
XmnI         1554  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1731  GAAGCTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BsaWI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        MaeII       
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfoI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     
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