Restriction map for pF1KB6321
 (Circular) (Six-base) MAPSORT of: pF1KB6321.seq  Check: 2529  from: 1  to: 3896



pF1KB6321  3896 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:    934     934 
   Size:       3896
 
Acc65I G'GTAC_C

Cuts at:    912     912 
   Size:       3896
 
AclI AA'CG_TT

Cuts at:   3736    3736 
   Size:       3896
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1721    2153    2900    3168    1721 
   Size:        432     747     268    2449

  Fragments arranged by size: 

                2449     747     432     268
 
AfeI AGC'GCT

Cuts at:    446     612     446 
   Size:        166    3730
 
AflIII A'CryG_T

Cuts at:   1248    2352    3329    1248 
   Size:       1104     977    1815

  Fragments arranged by size: 

                1815    1104     977
 
AgeI A'CCGG_T

Cuts at:   2284    2284 
   Size:       3896
 
AlwNI CAG_nnn'CTG

Cuts at:   1201    2768    1201 
   Size:       1567    2329
 
ApaLI G'TGCA_C

Cuts at:   2666    2666 
   Size:       3896
 
ApoI r'AATT_y

Cuts at:    215     299     900    3249    3505     215 
   Size:         84     601    2349     256     606

  Fragments arranged by size: 

                2349     606     601     256      84
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3896
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3896
 
AvaI C'yCGr_G

Cuts at:    916     916 
   Size:       3896
 
BamHI G'GATC_C

Cuts at:    921     921 
   Size:       3896
 
BanI G'GyrC_C

Cuts at:    202     912    1326    1574    1609     202 
   Size:        710     414     248      35    2489

  Fragments arranged by size: 

                2489     710     414     248      35
 
BanII G_rGCy'C

Cuts at:    910    1940     910 
   Size:       1030    2866
 
BbeI G_GCGC'C

Cuts at:   1330    1578    1330 
   Size:        248    3648
 
BciVI GTATCCnnnnn_n'

Cuts at:   1794    2555    3896    1794 
   Size:        761    1341    1794

  Fragments arranged by size: 

                1794    1341     761
 
BclI T'GATC_A

Cuts at:    815    1416     815 
   Size:        601    3295
 
BfrBI ATG'CAT

Cuts at:    596     596 
   Size:       3896
 
BglII A'GATC_T

Cuts at:   1411    3448    1411 
   Size:       2037    1859
 
BlpI GC'TnA_GC

Cuts at:    848    1008     848 
   Size:        160    3736
 
Bme1580I G_kGCm'C

Cuts at:   1521    1614    2670    1521 
   Size:         93    1056    2747

  Fragments arranged by size: 

                2747    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1108    1189    1524    3668    1108 
   Size:         81     335    2144    1336

  Fragments arranged by size: 

                2144    1336     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2263    2263 
   Size:       3896
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    111     131     111 
   Size:         20    3876
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1072    1951    2443    2741    1072 
   Size:        879     492     298    2227

  Fragments arranged by size: 

                2227     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3600    3600 
   Size:       3896
 
BsaAI yAC'GTr

Cuts at:   1249    1249 
   Size:       3896
 
BsaBI GATnn'nnATC

Cuts at:      6    1410    3447       6 
   Size:       1404    2037     455

  Fragments arranged by size: 

                2037    1404     455
 
BsaHI Gr'CG_yC

Cuts at:   1327    1575    2277    3773    1327 
   Size:        248     702    1496    1450

  Fragments arranged by size: 

                1496    1450     702     248
 
BsaWI w'CCGG_w

Cuts at:   1085    1606    2284    2558    2705    3116    1085 
   Size:        521     678     274     147     411    1865

  Fragments arranged by size: 

                1865     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    521    1321    2079    2656    3152     521 
   Size:        800     758     577     496    1265

  Fragments arranged by size: 

                1265     800     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1438    1484    2692     104 
   Size:       1334      46    1208    1308

  Fragments arranged by size: 

                1334    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    910    1688    1878    2670     910 
   Size:        778     190     792    2136

  Fragments arranged by size: 

                2136     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    246     246 
   Size:       3896
 
Bsp1286I G_dGCh'C

Cuts at:    910    1521    1614    1688    1878    1940    2670     910 
   Size:        611      93      74     190      62     730    2136

  Fragments arranged by size: 

                2136     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3116    3116 
   Size:       3896
 
BspHI T'CATG_A

Cuts at:   3072    3072 
   Size:       3896
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    946    1160    1462    1843     946 
   Size:        214     302     381    2999

  Fragments arranged by size: 

                2999     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2188    2242    3726    3891      50 
   Size:       2138      54    1484     165      55

  Fragments arranged by size: 

                2138    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    383     505    1808     383 
   Size:        122    1303    2471

  Fragments arranged by size: 

                2471    1303     122
 
BsrFI r'CCGG_y

Cuts at:   1894    2075    2284    1894 
   Size:        181     209    3506

  Fragments arranged by size: 

                3506     209     181
 
BsrGI T'GTAC_A

Cuts at:    512     512 
   Size:       3896
 
BssSI C'ACGA_G

Cuts at:    826    2167    2525     826 
   Size:       1341     358    2197

  Fragments arranged by size: 

                2197    1341     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1273    1273 
   Size:       3896
 
BstBI TT'CG_AA

Cuts at:   2257    2257 
   Size:       3896
 
BstYI r'GATC_y

Cuts at:      1     921    1388    1411    1746    1992    3004    3090    3102 
   Size:        920     467      23     335     246    1012      86      12

Cuts at:   3102    3448       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     920     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    179     707     179 
   Size:        528    3368
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1846    2027    2190    1846 
   Size:        181     163    3552

  Fragments arranged by size: 

                3552     181     163
 
BtsI GCAGTG_nn'

Cuts at:    336    1139     336 
   Size:        803    3093
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    579     614     579 
   Size:         35    3861
 
DraI TTT'AAA

Cuts at:    895     895 
   Size:       3896
 
DrdI GACnn_nn'nnGTC

Cuts at:    666    1602    2460     666 
   Size:        936     858    2102

  Fragments arranged by size: 

                2102     936     858
 
EaeI y'GGCC_r

Cuts at:   1481    1655    2046    2073    3418    1481 
   Size:        174     391      27    1345    1959

  Fragments arranged by size: 

                1959    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1481    1481 
   Size:       3896
 
EarI CTCTTCn'nnn_

Cuts at:   1919    2129    1919 
   Size:        210    3686
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2104    2414    2560    3706    2104 
   Size:        310     146    1146    2294

  Fragments arranged by size: 

                2294    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    908     908 
   Size:       3896
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    111     131    1721    2153    2900    3168     111 
   Size:         20    1590     432     747     268     839

  Fragments arranged by size: 

                1590     839     747     432     268      20
 
EcoO109I rG'GnC_Cy

Cuts at:   1035    1035 
   Size:       3896
 
EcoRI G'AATT_C

Cuts at:    900     900 
   Size:       3896
 
EcoRV GAT'ATC

Cuts at:    456     456 
   Size:       3896
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1915    1915 
   Size:       3896
 
FspI TGC'GCA

Cuts at:   1677    1677 
   Size:       3896
 
HaeII r_GCGC'y

Cuts at:    448     614    1330    1578    2600    3579     448 
   Size:        166     716     248    1022     979     765

  Fragments arranged by size: 

                1022     979     765     716     248     166
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    245     277    1738    1768    1770    1800     245 
   Size:         32    1461      30       2      30    2341

  Fragments arranged by size: 

                2341    1461      32      30      30       2
 
HincII GTy'rAC

Cuts at:    935     935 
   Size:       3896
 
HindIII A'AGCT_T

Cuts at:    268     464     268 
   Size:        196    3700
 
KasI G'GCGC_C

Cuts at:   1326    1574    1326 
   Size:        248    3648
 
KpnI G_GTAC'C

Cuts at:    916     916 
   Size:       3896
 
MluI A'CGCG_T

Cuts at:   3329    3329 
   Size:       3896
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2567    2751    2567 
   Size:        184    3712
 
MscI TGG'CCA

Cuts at:   1657    1657 
   Size:       3896
 
MslI CAynn'nnrTG

Cuts at:   1885    2012    1885 
   Size:        127    3769
 
MspA1I CmG'CkG

Cuts at:    619    1007    1321    1681    2694    2939     619 
   Size:        388     314     360    1013     245    1576

  Fragments arranged by size: 

                1576    1013     388     360     314     245
 
NaeI GCC'GGC

Cuts at:   2077    2077 
   Size:       3896
 
NarI GG'CG_CC

Cuts at:   1327    1575    1327 
   Size:        248    3648
 
NcoI C'CATG_G

Cuts at:    179     707     179 
   Size:        528    3368
 
NgoMIV G'CCGG_C

Cuts at:   2075    2075 
   Size:       3896
 
NsiI A_TGCA'T

Cuts at:    598     598 
   Size:       3896
 
NspI r_CATG'y

Cuts at:    199     596     949    1449    2356     199 
   Size:        397     353     500     907    1739

  Fragments arranged by size: 

                1739     907     500     397     353
 
PciI A'CATG_T

Cuts at:   2352    2352 
   Size:       3896
 
PfoI T'CCnGG_A

Cuts at:    127     127 
   Size:       3896
 
PmeI GTTT'AAAC

Cuts at:    895     895 
   Size:       3896
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    365     397    3035    3067     365 
   Size:         32    2638      32    1194

  Fragments arranged by size: 

                2638    1194      32      32
 
PsiI TTA'TAA

Cuts at:    305     305 
   Size:       3896
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    770     802     770 
   Size:         32    3864
 
PstI C_TGCA'G

Cuts at:    618     943    1628     618 
   Size:        325     685    2886

  Fragments arranged by size: 

                2886     685     325
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3896
 
PvuII CAG'CTG

Cuts at:    619    1321    1681     619 
   Size:        702     360    2834

  Fragments arranged by size: 

                2834     702     360
 
RsrII CG'GwC_CG

Cuts at:   2091    2091 
   Size:       3896
 
SacI G_AGCT'C

Cuts at:    910     910 
   Size:       3896
 
SalI G'TCGA_C

Cuts at:    933     933 
   Size:       3896
 
SapI GCTCTTCn'nnn_

Cuts at:   1919    2129    1919 
   Size:        210    3686
 
SbfI CC_TGCA'GG

Cuts at:    943     943 
   Size:       3896
 
ScaI AGT'ACT

Cuts at:    695    3413     695 
   Size:       2718    1178
 
SfcI C'TryA_G

Cuts at:     33     614     939    1624    2617    2808      33 
   Size:        581     325     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     581     325     191
 
SfoI GGC'GCC

Cuts at:   1328    1576    1328 
   Size:        248    3648
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3896
 
SmaI CCC'GGG

Cuts at:    918     918 
   Size:       3896
 
SmlI C'TyrA_G

Cuts at:   1051    1966    2458    2720    1051 
   Size:        915     492     262    2227

  Fragments arranged by size: 

                2227     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3896
 
SphI G_CATG'C

Cuts at:    949    1449     949 
   Size:        500    3396
 
StyI C'CwwG_G

Cuts at:    179     707    1030    1384     179 
   Size:        528     323     354    2691

  Fragments arranged by size: 

                2691     528     354     323
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1479    1612    2071    2280    1479 
   Size:        133     459     209    3095

  Fragments arranged by size: 

                3095     459     209     133
 
TatI w'GTAC_w

Cuts at:    512     693    3411     512 
   Size:        181    2718     997

  Fragments arranged by size: 

                2718     997     181
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3413    3445    3413 
   Size:         32    3864
 
Tth111I GACn'n_nGTC

Cuts at:   1693    1693 
   Size:       3896
 
XbaI T'CTAG_A

Cuts at:    927     927 
   Size:       3896
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1391    1391 
   Size:       3896
 
XmaI C'CCGG_G

Cuts at:    916     916 
   Size:       3896
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NsiI     NspI     PciI     PfoI     PmeI     PpiI     PsiI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI     BglI    BmgBI
     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI   DraIII    EcoNI     FseI
    FspAI     HpaI     MfeI     NdeI     NheI     NotI     NruI     PacI
     PasI    PflMI     PmlI    PpuMI    PshAI   PspOMI    PspXI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI     ZraI
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