Restriction map for pF1KB6916
##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MboII       MluI        MluNI       MroI        MscI        
MslI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          409  GTATAC               GT^[AC][GT]AC
AcsI          205  GAATTT               [GA]^AATT[CT]
AlwI          523  GGATC                GGATC(4/5)
Aor51HI        99  AGCGCT               AGC^GCT
ApoI          205  GAATTT               [GA]^AATT[CT]
BciVI         556  GTATCC               GTATCC(6/5)
BclI           57  TGATCA               T^GATCA
BcnI          165  CCCGG                CC^[GC]GG
BmyI            6  GGGCAC               G[AGT]GC[ACT]^C
BpmI           23  CTGGAG               CTGGAG(16/14)
BsiYI         274  CCCTCCTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          274  CCCTCCTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I        6  GGGCAC               G[AGT]GC[ACT]^C
Bst1107I      409  GTATAC               GTA^TAC
BstYI         523  GGATCT               [GA]^GATC[CT]
BstZ17I       409  GTATAC               GTA^TAC
Cac8I           4  GCGGGC               GC[ACGT]^[ACGT]GC
Cfr13I        333  GGCCC                G^G[ACGT]CC
DraI          486  TTTAAA               TTT^AAA
Eco47III       99  AGCGCT               AGC^GCT
FbaI           57  TGATCA               T^GATCA
HaeII          99  AGCGCT               [GA]GCGC^[CT]
HaeIII        333  GGCC                 GG^CC
MaeIII        381  GTCAC                ^GT[ACGT]AC
MfeI          233  CAATTG               C^AATTG
MflI          523  GGATCT               [GA]^GATC[CT]
MspA1I        518  CAGCTG               C[AC]G^C[GT]G
MunI          233  CAATTG               C^AATTG
MwoI           91  GCGGCTGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          165  CCCGG                CC^[GC]GG
NdeI          430  CATATG               CA^TATG
NspI          506  ACATGC               [GA]CATG^[CT]
PleI          544  GAGTC                GAGTC(4/5)
PstI           95  CTGCAG               CTGCA^G
PvuII         518  CAGCTG               CAG^CTG
Sau96I        333  GGCCC                G^G[ACGT]CC
SfcI           95  CTGCAG               C^T[GA][CT]AG
TaqI          225  TCGA                 T^CGA
TfiI          366  GAATC                G^A[AT]TC
Tsp45I        381  GTCAC                ^GT[GC]AC
TthHB8I       225  TCGA                 T^CGA
XhoII         523  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BfaI          424  CTAG                 C^TAG
              604  CTAG                
BseRI         276  CTCCTC               GAGGAG(10/8)
              109  GAGGAG              
CfoI           43  GCGC                 GCG^C
              100  GCGC                
DpnII          58  GATC                 ^GATC
              524  GATC                
FauI            4  GCGGG                CCCGC(4/6)
               84  GCGGG               
HapII         166  CCGG                 C^CGG
              663  CCGG                
HhaI           43  GCGC                 GCG^C
              100  GCGC                
HinP1I         43  GCGC                 G^CGC
              100  GCGC                
HinfI         366  GAATC                G^A[ACGT]TC
              544  GAGTC               
HpaII         166  CCGG                 C^CGG
              663  CCGG                
MaeI          424  CTAG                 C^TAG
              604  CTAG                
MboI           58  GATC                 ^GATC
              524  GATC                
MspI          166  CCGG                 C^CGG
              663  CCGG                
NdeII          58  GATC                 ^GATC
              524  GATC                
Sau3AI         58  GATC                 ^GATC
              524  GATC                
SfaNI          40  GATGC                GCATC(5/9)
              345  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsrI        
BssKI       BstF5I      BstNI       DdeI        EcoRII      
Fnu4HI      FokI        ItaI        MnlI        MseI        
MvaI        NlaIII      ScrFI       Tru9I       TseI        
Tsp509I     
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