Restriction map for
pF1KB6916
(Circular) (Six-base) MAPSORT of: pF1KB6916.seq Check: 2635 from: 1 to: 3788
pF1KB6916 3788 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 518 826 518
Size: 308 3480
Acc65I G'GTAC_C
Cuts at: 804 804
Size: 3788
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 1613 2045 2792 3060 550
Size: 1063 432 747 268 1278
Fragments arranged by size:
1278 1063 747 432 268
AfeI AGC'GCT
Cuts at: 209 209
Size: 3788
AflIII A'CryG_T
Cuts at: 1140 2244 3221 1140
Size: 1104 977 1707
Fragments arranged by size:
1707 1104 977
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 1093 2660 1093
Size: 1567 2221
ApaLI G'TGCA_C
Cuts at: 2558 2558
Size: 3788
ApoI r'AATT_y
Cuts at: 313 792 3141 3397 313
Size: 479 2349 256 704
Fragments arranged by size:
2349 704 479 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 808 808
Size: 3788
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 804 1218 1466 1501 804
Size: 414 248 35 3091
Fragments arranged by size:
3091 414 248 35
BanII G_rGCy'C
Cuts at: 802 1832 802
Size: 1030 2758
BbeI G_GCGC'C
Cuts at: 1222 1470 1222
Size: 248 3540
BciVI GTATCCnnnnn_n'
Cuts at: 675 1686 2447 3788 675
Size: 1011 761 1341 675
Fragments arranged by size:
1341 1011 761 675
BclI T'GATC_A
Cuts at: 165 1308 165
Size: 1143 2645
BglII A'GATC_T
Cuts at: 1303 3340 1303
Size: 2037 1751
BlpI GC'TnA_GC
Cuts at: 900 900
Size: 3788
Bme1580I G_kGCm'C
Cuts at: 118 1413 1506 2562 118
Size: 1295 93 1056 1344
Fragments arranged by size:
1344 1295 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 438 1000 1081 1416 3560 438
Size: 562 81 335 2144 666
Fragments arranged by size:
2144 666 562 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 152
Size: 3788
Bpu10I CC'TnA_GC
Cuts at: 256 256
Size: 3788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1843 2335 2633 964
Size: 879 492 298 2119
Fragments arranged by size:
2119 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3492 3492
Size: 3788
BsaAI yAC'GTr
Cuts at: 1141 1141
Size: 3788
BsaBI GATnn'nnATC
Cuts at: 6 1302 3339 6
Size: 1296 2037 455
Fragments arranged by size:
2037 1296 455
BsaHI Gr'CG_yC
Cuts at: 1219 1467 2169 3665 1219
Size: 248 702 1496 1342
Fragments arranged by size:
1496 1342 702 248
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 125 155 125
Size: 30 3758
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 375 232
Size: 143 3645
BseYI C'CCAG_C
Cuts at: 1213 1971 2548 3044 1213
Size: 758 577 496 1957
Fragments arranged by size:
1957 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1330 1376 2584 104
Size: 1226 46 1208 1308
Fragments arranged by size:
1308 1226 1208 46
BsiHKAI G_wGCw'C
Cuts at: 802 1580 1770 2562 802
Size: 778 190 792 2028
Fragments arranged by size:
2028 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 118 802 1413 1506 1580 1770 1832 2562 118
Size: 684 611 93 74 190 62 730 1344
Fragments arranged by size:
1344 730 684 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 1052 1354 1735 838
Size: 214 302 381 2891
Fragments arranged by size:
2891 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2080 2134 3618 3783 50
Size: 2030 54 1484 165 55
Fragments arranged by size:
2030 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1700 1700
Size: 3788
BsrFI r'CCGG_y
Cuts at: 1786 1967 2176 1786
Size: 181 209 3398
Fragments arranged by size:
3398 209 181
BssSI C'ACGA_G
Cuts at: 2059 2417 2059
Size: 358 3430
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 1165
Size: 3788
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstYI r'GATC_y
Cuts at: 1 631 813 1280 1303 1638 1884 2896 2982
Size: 630 182 467 23 335 246 1012 86
Cuts at: 2982 2994 3340 1
Size: 12 346 449
Fragments arranged by size:
1012 630 467 449 346 335 246 182
86 23 12
BstZ17I GTA'TAC
Cuts at: 519 519
Size: 3788
BtgI C'CryG_G
Cuts at: 107 107
Size: 3788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1738 1919 2082 1738
Size: 181 163 3444
Fragments arranged by size:
3444 181 163
BtsI GCAGTG_nn'
Cuts at: 1031 1031
Size: 3788
DraI TTT'AAA
Cuts at: 596 787 596
Size: 191 3597
DrdI GACnn_nn'nnGTC
Cuts at: 1494 2352 1494
Size: 858 2930
EaeI y'GGCC_r
Cuts at: 1373 1547 1938 1965 3310 1373
Size: 174 391 27 1345 1851
Fragments arranged by size:
1851 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1373 1373
Size: 3788
EarI CTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1996 2306 2452 3598 1996
Size: 310 146 1146 2186
Fragments arranged by size:
2186 1146 310 146
EcoICRI GAG'CTC
Cuts at: 800 800
Size: 3788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 550 1613 2045 2792 3060 152
Size: 398 1063 432 747 268 880
Fragments arranged by size:
1063 880 747 432 398 268
EcoO109I rG'GnC_Cy
Cuts at: 927 927
Size: 3788
EcoRI G'AATT_C
Cuts at: 792 792
Size: 3788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1807 1807
Size: 3788
FspI TGC'GCA
Cuts at: 1569 1569
Size: 3788
HaeII r_GCGC'y
Cuts at: 211 1222 1470 2492 3471 211
Size: 1011 248 1022 979 528
Fragments arranged by size:
1022 1011 979 528 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1630 1660 1662 1692 1630
Size: 30 2 30 3726
Fragments arranged by size:
3726 30 30 2
HincII GTy'rAC
Cuts at: 827 827
Size: 3788
KasI G'GCGC_C
Cuts at: 1218 1466 1218
Size: 248 3540
KpnI G_GTAC'C
Cuts at: 808 808
Size: 3788
MfeI C'AATT_G
Cuts at: 341 341
Size: 3788
MluI A'CGCG_T
Cuts at: 3221 3221
Size: 3788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2643 2459
Size: 184 3604
MscI TGG'CCA
Cuts at: 1549 1549
Size: 3788
MslI CAynn'nnrTG
Cuts at: 1777 1904 1777
Size: 127 3661
MspA1I CmG'CkG
Cuts at: 628 899 1213 1573 2586 2831 628
Size: 271 314 360 1013 245 1585
Fragments arranged by size:
1585 1013 360 314 271 245
NaeI GCC'GGC
Cuts at: 1969 1969
Size: 3788
NarI GG'CG_CC
Cuts at: 1219 1467 1219
Size: 248 3540
NcoI C'CATG_G
Cuts at: 107 107
Size: 3788
NdeI CA'TA_TG
Cuts at: 539 539
Size: 3788
NgoMIV G'CCGG_C
Cuts at: 1967 1967
Size: 3788
NspI r_CATG'y
Cuts at: 618 841 1341 2248 618
Size: 223 500 907 2158
Fragments arranged by size:
2158 907 500 223
PciI A'CATG_T
Cuts at: 2244 2244
Size: 3788
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PstI C_TGCA'G
Cuts at: 207 835 1520 207
Size: 628 685 2475
Fragments arranged by size:
2475 685 628
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 628 1213 1573 628
Size: 585 360 2843
Fragments arranged by size:
2843 585 360
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 802 802
Size: 3788
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SfcI C'TryA_G
Cuts at: 33 203 831 1516 2509 2700 33
Size: 170 628 685 993 191 1121
Fragments arranged by size:
1121 993 685 628 191 170
SfoI GGC'GCC
Cuts at: 1220 1468 1220
Size: 248 3540
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 943 1858 2350 2612 943
Size: 915 492 262 2119
Fragments arranged by size:
2119 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
StyI C'CwwG_G
Cuts at: 107 922 1276 107
Size: 815 354 2619
Fragments arranged by size:
2619 815 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1371 1504 1963 2172 1371
Size: 133 459 209 2987
Fragments arranged by size:
2987 459 209 133
TatI w'GTAC_w
Cuts at: 3303 3303
Size: 3788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 125 157 3305 3337 125
Size: 32 3148 32 576
Fragments arranged by size:
3148 576 32 32
Tth111I GACn'n_nGTC
Cuts at: 1585 1585
Size: 3788
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1283 1283
Size: 3788
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI