Restriction map for pF1KB5930
 (Circular) (Six-base) MAPSORT of: pF1KB5930.seq  Check: 4207  from: 1  to: 4727



pF1KB5930  4727 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    355     693     827     355 
   Size:        338     134    4255

  Fragments arranged by size: 

                4255     338     134
 
AccI GT'mk_AC

Cuts at:   1765    1765 
   Size:       4727
 
Acc65I G'GTAC_C

Cuts at:   1743    1743 
   Size:       4727
 
AclI AA'CG_TT

Cuts at:   4567    4567 
   Size:       4727
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    301     553     577     892    2552    2984    3731    3999     301 
   Size:        252      24     315    1660     432     747     268    1029

  Fragments arranged by size: 

                1660    1029     747     432     315     268     252      24
            
 
AflIII A'CryG_T

Cuts at:   1571    2079    3183    4160    1571 
   Size:        508    1104     977    2138

  Fragments arranged by size: 

                2138    1104     977     508
 
AgeI A'CCGG_T

Cuts at:   3115    3115 
   Size:       4727
 
AlwNI CAG_nnn'CTG

Cuts at:   1160    1246    2032    3599    1160 
   Size:         86     786    1567    2288

  Fragments arranged by size: 

                2288    1567     786      86
 
ApaI G_GGCC'C

Cuts at:    719    1430     719 
   Size:        711    4016
 
ApaLI G'TGCA_C

Cuts at:    986    3497     986 
   Size:       2511    2216
 
ApoI r'AATT_y

Cuts at:   1731    4080    4336    1731 
   Size:       2349     256    2122

  Fragments arranged by size: 

                2349    2122     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4727
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4727
 
AvaI C'yCGr_G

Cuts at:    687    1179    1636    1747     687 
   Size:        492     457     111    3667

  Fragments arranged by size: 

                3667     492     457     111
 
BamHI G'GATC_C

Cuts at:   1752    1752 
   Size:       4727
 
BanI G'GyrC_C

Cuts at:   1035    1743    2157    2405    2440    1035 
   Size:        708     414     248      35    3322

  Fragments arranged by size: 

                3322     708     414     248      35
 
BanII G_rGCy'C

Cuts at:    719    1030    1081    1189    1337    1430    1546    1597    1705 
   Size:        311      51     108     148      93     116      51     108

Cuts at:   1705    1741    2771     719 
   Size:         36    1030    2675

  Fragments arranged by size: 

                2675    1030     311     148     116     108     108      93
                  51      51      36
 
BbeI G_GCGC'C

Cuts at:   2161    2409    2161 
   Size:        248    4479
 
BbsI GAAGACnn'nnnn_

Cuts at:    542     840     880     542 
   Size:        298      40    4389

  Fragments arranged by size: 

                4389     298      40
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     523     557      90 
   Size:         34     399      34    4260

  Fragments arranged by size: 

                4260     399      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2625    3386    4727    2625 
   Size:        761    1341    2625

  Fragments arranged by size: 

                2625    1341     761
 
BclI T'GATC_A

Cuts at:   2247    2247 
   Size:       4727
 
BglII A'GATC_T

Cuts at:   2242    4279    2242 
   Size:       2037    2690
 
BlpI GC'TnA_GC

Cuts at:   1188    1839    1188 
   Size:        651    4076
 
Bme1580I G_kGCm'C

Cuts at:    719     990    1040    1148    1430    2352    2445    3501     719 
   Size:        271      50     108     282     922      93    1056    1945

  Fragments arranged by size: 

                1945    1056     922     282     271     108      93      50
            
 
BmrI ACTGGGnnnn_n'

Cuts at:    777    1491    1939    2020    2355    4499     777 
   Size:        714     448      81     335    2144    1005

  Fragments arranged by size: 

                2144    1005     714     448     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    312    1056    1560    3094     312 
   Size:        744     504    1534    1945

  Fragments arranged by size: 

                1945    1534     744     504
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    133     181     691     768     885     987    1030    1044    1069 
   Size:         48     510      77     117     102      43      14      25

Cuts at:   1069    1351    1538     133 
   Size:        282     187    3322

  Fragments arranged by size: 

                3322     510     282     187     117     102      77      48
                  43      25      14
 
Bpu10I CC'TnA_GC

Cuts at:    926     926 
   Size:       4727
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    630    1903    2782    3274    3572     630 
   Size:       1273     879     492     298    1785

  Fragments arranged by size: 

                1785    1273     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    593    1530    4431     593 
   Size:        937    2901     889

  Fragments arranged by size: 

                2901     937     889
 
BsaAI yAC'GTr

Cuts at:   2080    2080 
   Size:       4727
 
BsaBI GATnn'nnATC

Cuts at:      6    2241    4278       6 
   Size:       2235    2037     455

  Fragments arranged by size: 

                2235    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2158    2406    3108    4604    2158 
   Size:        248     702    1496    2281

  Fragments arranged by size: 

                2281    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1916    2437    3115    3389    3536    3947    1916 
   Size:        521     678     274     147     411    2696

  Fragments arranged by size: 

                2696     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    636     652     720     805     820    1216     636 
   Size:         16      68      85      15     396    4147

  Fragments arranged by size: 

                4147     396      85      68      16      15
 
BseYI C'CCAG_C

Cuts at:    119     158     238     919    1014    1153    1305    1336    1486 
   Size:         39      80     681      95     139     152      31     150

Cuts at:   1486    2152    2910    3487    3983     119 
   Size:        666     758     577     496     863

  Fragments arranged by size: 

                 863     758     681     666     577     496     152     150
                 139      95      80      39      31
 
BsiEI CG_ry'CG

Cuts at:    104    2269    2315    3523     104 
   Size:       2165      46    1208    1308

  Fragments arranged by size: 

                2165    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    990    1741    2519    2709    3501     990 
   Size:        751     778     190     792    2216

  Fragments arranged by size: 

                2216     792     778     751     190
 
Bsp1286I G_dGCh'C

Cuts at:    719     990    1030    1040    1081    1148    1189    1337    1430 
   Size:        271      40      10      41      67      41     148      93

Cuts at:   1430    1546    1597    1705    1741    2352    2445    2519    2709 
   Size:        116      51     108      36     611      93      74     190

Cuts at:   2709    2771    3501     719 
   Size:         62     730    1945

  Fragments arranged by size: 

                1945     730     611     271     190     148     116     108
                  93      93      74      67      62      51      41      41
                  40      36      10
 
BspEI T'CCGG_A

Cuts at:   3947    3947 
   Size:       4727
 
BspHI T'CATG_A

Cuts at:   3903    3903 
   Size:       4727
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    355     693     827    1777    1991    2293    2674     355 
   Size:        338     134     950     214     302     381    2408

  Fragments arranged by size: 

                2408     950     381     338     302     214     134
 
BsrBI CCG'CTC

Cuts at:     50     930    3019    3073    4557    4722      50 
   Size:        880    2089      54    1484     165      55

  Fragments arranged by size: 

                2089    1484     880     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2639    2639 
   Size:       4727
 
BsrFI r'CCGG_y

Cuts at:    465    1508    1555    2725    2906    3115     465 
   Size:       1043      47    1170     181     209    2077

  Fragments arranged by size: 

                2077    1170    1043     209     181      47
 
BsrGI T'GTAC_A

Cuts at:    947    1055     947 
   Size:        108    4619
 
BssSI C'ACGA_G

Cuts at:   2998    3356    2998 
   Size:        358    4369
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2104    2104 
   Size:       4727
 
BstBI TT'CG_AA

Cuts at:   3088    3088 
   Size:       4727
 
BstEII G'GTnAC_C

Cuts at:   1511    1511 
   Size:       4727
 
BstXI CCAn_nnnn'nTGG

Cuts at:    433    1274     433 
   Size:        841    3886
 
BstYI r'GATC_y

Cuts at:      1    1752    2219    2242    2577    2823    3835    3921    3933 
   Size:       1751     467      23     335     246    1012      86      12

Cuts at:   3933    4279       1 
   Size:        346     449

  Fragments arranged by size: 

                1751    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:   1072    1072 
   Size:       4727
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2677    2858    3021    2677 
   Size:        181     163    4383

  Fragments arranged by size: 

                4383     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1970    1970 
   Size:       4727
 
DraI TTT'AAA

Cuts at:   1726    1726 
   Size:       4727
 
DrdI GACnn_nn'nnGTC

Cuts at:   2433    3291    2433 
   Size:        858    3869
 
EaeI y'GGCC_r

Cuts at:    374    1082    1162    1213    1506    1553    2312    2486    2877 
   Size:        708      80      51     293      47     759     174     391

Cuts at:   2877    2904    4249     374 
   Size:         27    1345     852

  Fragments arranged by size: 

                1345     852     759     708     391     293     174      80
                  51      47      27
 
EagI C'GGCC_G

Cuts at:   2312    2312 
   Size:       4727
 
EarI CTCTTCn'nnn_

Cuts at:    269    2750    2960     269 
   Size:       2481     210    2036

  Fragments arranged by size: 

                2481    2036     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1657    1679    2935    3245    3391    4537    1657 
   Size:         22    1256     310     146    1146    1847

  Fragments arranged by size: 

                1847    1256    1146     310     146      22
 
EcoICRI GAG'CTC

Cuts at:   1739    1739 
   Size:       4727
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    133     181     301     553     577     691     768     885     892 
   Size:         48     120     252      24     114      77     117       7

Cuts at:    892     987    1030    1044    1069    1351    1538    2552    2984 
   Size:         95      43      14      25     282     187    1014     432

Cuts at:   2984    3731    3999     133 
   Size:        747     268     861

  Fragments arranged by size: 

                1014     861     747     432     282     268     252     187
                 120     117     114      95      77      48      43      25
                  24      14       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:    949    1418     949 
   Size:        469    4258
 
EcoO109I rG'GnC_Cy

Cuts at:    715     743    1018    1353    1426    1427    1648    1866     715 
   Size:         28     275     335      73       1     221     218    3576

  Fragments arranged by size: 

                3576     335     275     221     218      73      28       1
            
 
EcoRI G'AATT_C

Cuts at:   1731    1731 
   Size:       4727
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1173    2746    1173 
   Size:       1573    3154
 
FseI GG_CCGG'CC

Cuts at:   1559    1559 
   Size:       4727
 
FspI TGC'GCA

Cuts at:   2508    2508 
   Size:       4727
 
HaeII r_GCGC'y

Cuts at:    525    2161    2409    3431    4410     525 
   Size:       1636     248    1022     979     842

  Fragments arranged by size: 

                1636    1022     979     842     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2569    2599    2601    2631    2569 
   Size:         30       2      30    4665

  Fragments arranged by size: 

                4665      30      30       2
 
HincII GTy'rAC

Cuts at:    396    1766     396 
   Size:       1370    3357
 
HindIII A'AGCT_T

Cuts at:    565     565 
   Size:       4727
 
KasI G'GCGC_C

Cuts at:   2157    2405    2157 
   Size:        248    4479
 
KpnI G_GTAC'C

Cuts at:   1747    1747 
   Size:       4727
 
MluI A'CGCG_T

Cuts at:   4160    4160 
   Size:       4727
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3398    3582    3398 
   Size:        184    4543
 
MscI TGG'CCA

Cuts at:   2488    2488 
   Size:       4727
 
MslI CAynn'nnrTG

Cuts at:    356    2716    2843     356 
   Size:       2360     127    2240

  Fragments arranged by size: 

                2360    2240     127
 
MspA1I CmG'CkG

Cuts at:    962    1061    1286    1405    1838    2152    2512    3525    3770 
   Size:         99     225     119     433     314     360    1013     245

Cuts at:   3770     962 
   Size:       1919

  Fragments arranged by size: 

                1919    1013     433     360     314     245     225     119
                  99
 
NaeI GCC'GGC

Cuts at:    467    1557    2908     467 
   Size:       1090    1351    2286

  Fragments arranged by size: 

                2286    1351    1090
 
NarI GG'CG_CC

Cuts at:   2158    2406    2158 
   Size:        248    4479
 
NgoMIV G'CCGG_C

Cuts at:    465    1555    2906     465 
   Size:       1090    1351    2286

  Fragments arranged by size: 

                2286    1351    1090
 
NspI r_CATG'y

Cuts at:    337     555    1780    2280    3187     337 
   Size:        218    1225     500     907    1877

  Fragments arranged by size: 

                1877    1225     907     500     218
 
PasI CC'CwG_GG

Cuts at:    605    1414    1431    1499     605 
   Size:        809      17      68    3833

  Fragments arranged by size: 

                3833     809      68      17
 
PciI A'CATG_T

Cuts at:   3183    3183 
   Size:       4727
 
PflMI CCAn_nnn'nTGG

Cuts at:    372    1160    1677     372 
   Size:        788     517    3422

  Fragments arranged by size: 

                3422     788     517
 
PfoI T'CCnGG_A

Cuts at:    215    1442     215 
   Size:       1227    3500
 
PmeI GTTT'AAAC

Cuts at:   1726    1726 
   Size:       4727
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3866    3898    3866 
   Size:         32    4695
 
PpuMI rG'GwC_Cy

Cuts at:    743    1018    1353    1648     743 
   Size:        275     335     295    3822

  Fragments arranged by size: 

                3822     335     295     275
 
PshAI GACnn'nnGTC

Cuts at:   1378    1378 
   Size:       4727
 
PspOMI G'GGCC_C

Cuts at:    715    1426     715 
   Size:        711    4016
 
PspXI vC'TCGA_Gb

Cuts at:    687     687 
   Size:       4727
 
PstI C_TGCA'G

Cuts at:    304     584     704     961    1097    1285    1606    1774    2459 
   Size:        280     120     257     136     188     321     168     685

Cuts at:   2459     304 
   Size:       2572

  Fragments arranged by size: 

                2572     685     321     280     257     188     168     136
                 120
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4727
 
PvuII CAG'CTG

Cuts at:   1061    1286    1405    2152    2512    1061 
   Size:        225     119     747     360    3276

  Fragments arranged by size: 

                3276     747     360     225     119
 
RsrII CG'GwC_CG

Cuts at:   2922    2922 
   Size:       4727
 
SacI G_AGCT'C

Cuts at:   1741    1741 
   Size:       4727
 
SalI G'TCGA_C

Cuts at:   1764    1764 
   Size:       4727
 
SanDI GG'GwC_CC

Cuts at:   1018    1018 
   Size:       4727
 
SapI GCTCTTCn'nnn_

Cuts at:    269    2750    2960     269 
   Size:       2481     210    2036

  Fragments arranged by size: 

                2481    2036     210
 
SbfI CC_TGCA'GG

Cuts at:   1774    1774 
   Size:       4727
 
ScaI AGT'ACT

Cuts at:   4244    4244 
   Size:       4727
 
SfcI C'TryA_G

Cuts at:     33     300     580     700     957    1093    1281    1602    1770 
   Size:        267     280     120     257     136     188     321     168

Cuts at:   1770    2455    3448    3639      33 
   Size:        685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     321     280     267     257     191
                 188     168     136     120
 
SfoI GGC'GCC

Cuts at:   2159    2407    2159 
   Size:        248    4479
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4727
 
SmaI CCC'GGG

Cuts at:   1638    1749    1638 
   Size:        111    4616
 
SmlI C'TyrA_G

Cuts at:    645     687    1882    2797    3289    3551     645 
   Size:         42    1195     915     492     262    1821

  Fragments arranged by size: 

                1821    1195     915     492     262      42
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4727
 
SphI G_CATG'C

Cuts at:    337    1780    2280     337 
   Size:       1443     500    2784

  Fragments arranged by size: 

                2784    1443     500
 
StuI AGG'CCT

Cuts at:   1346    1346 
   Size:       4727
 
StyI C'CwwG_G

Cuts at:   1861    2215    1861 
   Size:        354    4373
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    969     973    1082    1665    2310    2443    2902    3111     969 
   Size:          4     109     583     645     133     459     209    2585

  Fragments arranged by size: 

                2585     645     583     459     209     133     109       4
            
 
TatI w'GTAC_w

Cuts at:    584     947    1055    4242     584 
   Size:        363     108    3187    1069

  Fragments arranged by size: 

                3187    1069     363     108
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    735     767    4244    4276     735 
   Size:         32    3477      32    1186

  Fragments arranged by size: 

                3477    1186      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    175     766     783    2524     175 
   Size:        591      17    1741    2378

  Fragments arranged by size: 

                2378    1741     591      17
 
XbaI T'CTAG_A

Cuts at:   1758    1758 
   Size:       4727
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1011    2222    1011 
   Size:       1211    3516
 
XhoI C'TCGA_G

Cuts at:    687     687 
   Size:       4727
 
XmaI C'CCGG_G

Cuts at:   1636    1747    1636 
   Size:        111    4616
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     AscI
    AvrII     BaeI    BbvCI    BfrBI     BglI    BmgBI     BmtI    BsaXI
     BsgI    BsiWI     BsmI    BsmBI   BssHII  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoRV    FspAI     HpaI     MfeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PmlI     PsiI     PsrI
    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XmnI     ZraI
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