Restriction map for pF1KB6663
 (Linear) (Six-base) MAPSORT of: KIBB6663.seq  Check: 2611  from: 1  to: 1272



KIBB6663.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     115    1272 
   Size:        115    1157
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      83     115     586     618    1272 
   Size:         83      32     471      32     654

  Fragments arranged by size: 

                 654     471      83      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     985    1272 
   Size:        985     287
 
ApoI r'AATT_y

Cuts at:      0     178    1272 
   Size:        178    1094
 
AseI AT'TA_AT

Cuts at:      0     440    1272 
   Size:        440     832
 
AvaI C'yCGr_G

Cuts at:      0    1058    1241    1272 
   Size:       1058     183      31

  Fragments arranged by size: 

                1058     183      31
 
BanII G_rGCy'C

Cuts at:      0     756    1272 
   Size:        756     516
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     761    1144    1272 
   Size:        761     383     128

  Fragments arranged by size: 

                 761     383     128
 
BclI T'GATC_A

Cuts at:      0     227    1272 
   Size:        227    1045
 
BglI GCCn_nnn'nGGC

Cuts at:      0     950    1272 
   Size:        950     322
 
BlpI GC'TnA_GC

Cuts at:      0     564    1272 
   Size:        564     708
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     730    1272 
   Size:        730     542
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     770    1272 
   Size:         85     685     502

  Fragments arranged by size: 

                 685     502      85
 
Bpu10I CC'TnA_GC

Cuts at:      0     954    1272 
   Size:        954     318
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204    1272 
   Size:        204    1068
 
BsaAI yAC'GTr

Cuts at:      0      48    1272 
   Size:         48    1224
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      83     113     586     616    1146    1176    1272 
   Size:         83      30     473      30     530      30      96

  Fragments arranged by size: 

                 530     473      96      83      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     635     704    1028    1118    1272 
   Size:        635      69     324      90     154

  Fragments arranged by size: 

                 635     324     154      90      69
 
BseYI C'CCAG_C

Cuts at:      0     774    1272 
   Size:        774     498
 
BsmI GAATG_Cn'

Cuts at:      0    1025    1272 
   Size:       1025     247
 
Bsp1286I G_dGCh'C

Cuts at:      0     756    1272 
   Size:        756     516
 
BspHI T'CATG_A

Cuts at:      0     218    1272 
   Size:        218    1054
 
BsrBI CCG'CTC

Cuts at:      0      24     600    1272 
   Size:         24     576     672

  Fragments arranged by size: 

                 672     576      24
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1116    1272 
   Size:       1116     156
 
BstEII G'GTnAC_C

Cuts at:      0    1087    1272 
   Size:       1087     185
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     166     669    1272 
   Size:        166     503     603

  Fragments arranged by size: 

                 603     503     166
 
BstYI r'GATC_y

Cuts at:      0    1075    1272 
   Size:       1075     197
 
BtgI C'CryG_G

Cuts at:      0     558     939    1082    1146    1272 
   Size:        558     381     143      64     126

  Fragments arranged by size: 

                 558     381     143     126      64
 
DraIII CAC_nnn'GTG

Cuts at:      0     895    1272 
   Size:        895     377
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     857    1272 
   Size:        857     415
 
EaeI y'GGCC_r

Cuts at:      0     150    1225    1272 
   Size:        150    1075      47

  Fragments arranged by size: 

                1075     150      47
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     770    1272 
   Size:         85     685     502

  Fragments arranged by size: 

                 685     502      85
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     410     701    1272 
   Size:        410     291     571

  Fragments arranged by size: 

                 571     410     291
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     402    1063    1096    1195    1258    1272 
   Size:         54     348     661      33      99      63      14

  Fragments arranged by size: 

                 661     348      99      63      54      33      14
 
EcoRI G'AATT_C

Cuts at:      0     178    1272 
   Size:        178    1094
 
HaeII r_GCGC'y

Cuts at:      0     365     558    1272 
   Size:        365     193     714

  Fragments arranged by size: 

                 714     365     193
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     101     133     891     923    1272 
   Size:        101      32     758      32     349

  Fragments arranged by size: 

                 758     349     101      32      32
 
HincII GTy'rAC

Cuts at:      0     120    1272 
   Size:        120    1152
 
MfeI C'AATT_G

Cuts at:      0     473    1272 
   Size:        473     799
 
MscI TGG'CCA

Cuts at:      0     152    1272 
   Size:        152    1120
 
MslI CAynn'nnrTG

Cuts at:      0     127     223    1272 
   Size:        127      96    1049

  Fragments arranged by size: 

                1049     127      96
 
MspA1I CmG'CkG

Cuts at:      0      81     627    1191    1272 
   Size:         81     546     564      81

  Fragments arranged by size: 

                 564     546      81      81
 
NcoI C'CATG_G

Cuts at:      0     558     939    1082    1146    1272 
   Size:        558     381     143      64     126

  Fragments arranged by size: 

                 558     381     143     126      64
 
NspI r_CATG'y

Cuts at:      0     132     711    1053    1272 
   Size:        132     579     342     219

  Fragments arranged by size: 

                 579     342     219     132
 
PasI CC'CwG_GG

Cuts at:      0     841    1272 
   Size:        841     431
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     748     895     985    1015    1180    1272 
   Size:        748     147      90      30     165      92

  Fragments arranged by size: 

                 748     165     147      92      90      30
 
PfoI T'CCnGG_A

Cuts at:      0     747    1029    1272 
   Size:        747     282     243

  Fragments arranged by size: 

                 747     282     243
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      83     115     586     618    1272 
   Size:         83      32     471      32     654

  Fragments arranged by size: 

                 654     471      83      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     402    1096    1272 
   Size:        402     694     176

  Fragments arranged by size: 

                 694     402     176
 
PspXI vC'TCGA_Gb

Cuts at:      0    1241    1272 
   Size:       1241      31
 
PstI C_TGCA'G

Cuts at:      0     813    1272 
   Size:        813     459
 
PvuII CAG'CTG

Cuts at:      0     627    1272 
   Size:        627     645
 
SanDI GG'GwC_CC

Cuts at:      0     402    1272 
   Size:        402     870
 
SfcI C'TryA_G

Cuts at:      0     809    1272 
   Size:        809     463
 
SmlI C'TyrA_G

Cuts at:      0     183    1241    1272 
   Size:        183    1058      31

  Fragments arranged by size: 

                1058     183      31
 
SphI G_CATG'C

Cuts at:      0     711    1053    1272 
   Size:        711     342     219

  Fragments arranged by size: 

                 711     342     219
 
StyI C'CwwG_G

Cuts at:      0     159     545     558     939    1082    1146    1272 
   Size:        159     386      13     381     143      64     126

  Fragments arranged by size: 

                 386     381     159     143     126      64      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     139     896    1106    1224    1272 
   Size:        139     757     210     118      48

  Fragments arranged by size: 

                 757     210     139     118      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1146    1178    1272 
   Size:       1146      32      94

  Fragments arranged by size: 

                1146      94      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      45     946    1153    1272 
   Size:         45     901     207     119

  Fragments arranged by size: 

                 901     207     119      45
 
XhoI C'TCGA_G

Cuts at:      0    1241    1272 
   Size:       1241      31
 
XmnI GAAnn'nnTTC

Cuts at:      0      95     578    1272 
   Size:         95     483     694

  Fragments arranged by size: 

                 694     483      95
 

 Enzymes that do cut:

     AhdI     AloI    AlwNI     ApoI     AseI     AvaI    BanII    BciVI
     BclI     BglI     BlpI     BmrI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaXI    BseRI    BseYI     BsmI Bsp1286I    BspHI    BsrBI   BstAPI
   BstEII    BstXI    BstYI     BtgI   DraIII     DrdI     EaeI  Eco57MI
    EcoNI EcoO109I    EcoRI    HaeII    Hin4I   HincII     MfeI     MscI
     MslI   MspA1I     NcoI     NspI     PasI    PflMI     PfoI     PpiI
    PpuMI    PspXI     PstI    PvuII    SanDI     SfcI     SmlI     SphI
     StyI    TaqII     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BfrBI    BglII Bme1580I    BmgBI     BmtI     BplI     BsaI    BsaBI
    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI     EagI     EarI
     EciI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PmlI    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com