Restriction map for pF1KE1305
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflIII      AgeI        Alw44I      Aor51HI     ApaLI       
AscI        Asp718I     AviII       BamHI       BbeI        
BbrPI       BlpI        Bpu1102I    BsaBI       BsaHI       
BsaWI       BseAI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspLU11I    BsrBI       BsrFI       
BssHII      Bst1107I    BstBI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraIII      DsaI        
Eco47III    EcoRI       EcoRV       FseI        FspI        
Hin1I       HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SpeI        Sse8387I    SwaI        
Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI         3420  GTCTAC               GT^[AC][GT]AC
AhdI         3136  GACTGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1195  CCTGAGG              CC^T[ACGT]AGG
AseI         2362  ATTAAT               AT^TAAT
AsnI         2362  ATTAAT               AT^TAAT
AspEI        3136  GACTGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2143  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          112  CCCGGG               C^[CT]CG[GA]G
AvrII        3789  CCTAGG               C^CTAGG
BalI         1650  TGGCCA               TGG^CCA
BanI         3532  GGTGCC               G^G[CT][GA]CC
BbvCI         959  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1167  CGATTTTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2736  GGATAC               GTATCC(6/5)
BlnI         3789  CCTAGG               C^CTAGG
BsaAI         640  TACGTA               [CT]AC^GT[GA]
BsaI         2486  GGTCTC               GGTCTC(1/5)
BsiEI          93  CGGCCG               CG[GA][CT]^CG
BsoBI         112  CCCGGG               C^[CT]CG[GA]G
BspMI        2664  GCAGGT               ACCTGC(4/8)
BstAPI       3907  GCAAGGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        304  GGTTACC              G^GT[ACGT]ACC
BstPI         304  GGTTACC              G^GT[ACGT]ACC
Bsu36I       1195  CCTGAGG              CC^T[ACGT]AGG
DraI          441  TTTAAA               TTT^AAA
DrdI         1986  GACTTCAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           93  CGGCCG               C^GGCCG
Eam1105I     3136  GACTGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          93  CGGCCG               C^GGCCG
Eco52I         93  CGGCCG               C^GGCCG
Eco81I       1195  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       304  GGTTACC              G^GT[ACGT]ACC
FauI          134  GCGGG                CCCGC(4/6)
HgaI          190  GCGTC                GACGC(5/10)
HindIII      2440  AAGCTT               A^AGCTT
MfeI         3506  CAATTG               C^AATTG
MluNI        1650  TGGCCA               TGG^CCA
MscI         1650  TGGCCA               TGG^CCA
MunI         3506  CAATTG               C^AATTG
NruI            4  TCGCGA               TCG^CGA
PflFI        2143  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        2362  ATTAAT               AT^TAAT
PstI         2677  CTGCAG               CTGCA^G
PvuII        2932  CAGCTG               CAG^CTG
SmaI          112  CCCGGG               CCC^GGG
SnaBI         640  TACGTA               TAC^GTA
SspI         1922  AATATT               AAT^ATT
Tth111I      2143  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2373  TCTAGA               T^CTAGA
XmaCI         112  CCCGGG               C^CCGGG
XmaI          112  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AflII        1117  CTTAAG               C^TTAAG
             2659  CTTAAG              
AlwNI         755  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1991  CAGAGTCTG           
ApaI          166  GGGCCC               GGGCC^C
             1367  GGGCCC              
Asp700I      2122  GAATATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3931  GAAGTTATTC          
AspHI          12  GAGCAC               G[AT]GC[AT]^C
             1141  GAGCAC              
BclI         1158  TGATCA               T^GATCA
             2088  TGATCA              
BfrI         1117  CTTAAG               C^TTAAG
             2659  CTTAAG              
BglI           58  GCCAACAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              109  GCCCCCGGGGC         
BpmI         1463  CTCCAG               CTGGAG(16/14)
              147  CTGGAG              
BsiHKAI        12  GAGCAC               G[AT]GC[AT]^C
             1141  GAGCAC              
Bsp1407I       20  TGTACA               T^GTACA
              390  TGTACA              
BspHI        1098  TCATGA               T^CATGA
             1352  TCATGA              
BsrDI         523  GCAATG               GCAATG(2/0)
             2325  GCAATG              
BsrGI          20  TGTACA               T^GTACA
              390  TGTACA              
BssSI        1442  CACGAG               CACGAG(-5/-1)
             1658  CTCGTG              
BstXI         170  CCAGGCCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1050  CCACGTTAGTGG        
FbaI         1158  TGATCA               T^GATCA
             2088  TGATCA              
HaeII          45  GGCGCT               [GA]GCGC^[CT]
              240  AGCGCC              
HincII       2010  GTTGAC               GT[CT]^[GA]AC
             4026  GTCAAC              
HindII       2010  GTTGAC               GT[CT]^[GA]AC
             4026  GTCAAC              
MspA1I        104  CCGCTG               C[AC]G^C[GT]G
             2932  CAGCTG              
NdeI         1664  CATATG               CA^TATG
             3796  CATATG              
PshAI        1072  GACAGTAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2895  GACCATAGTC          
RcaI         1098  TCATGA               T^CATGA
             1352  TCATGA              
SspBI          20  TGTACA               T^GTACA
              390  TGTACA              
XmnI         2122  GAATATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3931  GAAGTTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AvaII       BanII       BbsI        
BbvI        BcnI        BfaI        BglII       BmyI        
BpuAI       BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     EcoT22I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SphI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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