Restriction map for pF1KE1305
 (Linear) (Six-base) MAPSORT of: KIEE1305.seq  Check: 6702  from: 1  to: 4080



KIEE1305.orf

 With 182 enzymes: SgfI * 

                        March 1, 2013 10:20  ..

AccI GT'mk_AC

Cuts at:      0    3421    4080 
   Size:       3421     659
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1834    1973    2282    2515    2714    3083    3506    4061 
   Size:       1834     139     309     233     199     369     423     555

Cuts at:   4061    4080 
   Size:         19

  Fragments arranged by size: 

                1834     555     423     369     309     233     199     139
                  19
 
AflII C'TTAA_G

Cuts at:      0    1117    2659    4080 
   Size:       1117    1542    1421

  Fragments arranged by size: 

                1542    1421    1117
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3141    4080 
   Size:       3141     939
 
AleI CACnn'nnGTG

Cuts at:      0    1055    4080 
   Size:       1055    3025
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3619    4080 
   Size:       3619     461
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2834    2866    4080 
   Size:       2834      32    1214

  Fragments arranged by size: 

                2834    1214      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     760    1996    4080 
   Size:        760    1236    2084

  Fragments arranged by size: 

                2084    1236     760
 
ApaI G_GGCC'C

Cuts at:      0     170    1371    4080 
   Size:        170    1201    2709

  Fragments arranged by size: 

                2709    1201     170
 
ApoI r'AATT_y

Cuts at:      0     790    1032    1291    2471    2939    2949    3862    3964 
   Size:        790     242     259    1180     468      10     913     102

Cuts at:   3964    4080 
   Size:        116

  Fragments arranged by size: 

                1180     913     790     468     259     242     116     102
                  10
 
AseI AT'TA_AT

Cuts at:      0    2363    4080 
   Size:       2363    1717
 
AvaI C'yCGr_G

Cuts at:      0     112    4080 
   Size:        112    3968
 
AvrII C'CTAG_G

Cuts at:      0    3789    4080 
   Size:       3789     291
 
BanI G'GyrC_C

Cuts at:      0    3532    4080 
   Size:       3532     548
 
BanII G_rGCy'C

Cuts at:      0     170     596    1371    4080 
   Size:        170     426     775    2709

  Fragments arranged by size: 

                2709     775     426     170
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2055    2393    3307    3833    4080 
   Size:       2055     338     914     526     247

  Fragments arranged by size: 

                2055     914     526     338     247
 
BbvCI CC'TCA_GC

Cuts at:      0     960    4080 
   Size:        960    3120
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1156    1190    4080 
   Size:       1156      34    2890

  Fragments arranged by size: 

                2890    1156      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2730    4080 
   Size:       2730    1350
 
BclI T'GATC_A

Cuts at:      0    1158    2088    4080 
   Size:       1158     930    1992

  Fragments arranged by size: 

                1992    1158     930
 
BfrBI ATG'CAT

Cuts at:      0    1177    1888    3606    3806    4080 
   Size:       1177     711    1718     200     274

  Fragments arranged by size: 

                1718    1177     711     274     200
 
BglI GCCn_nnn'nGGC

Cuts at:      0      64     115    4080 
   Size:         64      51    3965

  Fragments arranged by size: 

                3965      64      51
 
BglII A'GATC_T

Cuts at:      0    1535    2161    2448    4080 
   Size:       1535     626     287    1632

  Fragments arranged by size: 

                1632    1535     626     287
 
Bme1580I G_kGCm'C

Cuts at:      0     170    1371    2340    4080 
   Size:        170    1201     969    1740

  Fragments arranged by size: 

                1740    1201     969     170
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1348    1374    1702    1765    1869    3223    4080 
   Size:       1348      26     328      63     104    1354     857

  Fragments arranged by size: 

                1354    1348     857     328     104      63      26
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168    1448    4080 
   Size:        168    1280    2632

  Fragments arranged by size: 

                2632    1280     168
 
Bpu10I CC'TnA_GC

Cuts at:      0     960    4080 
   Size:        960    3120
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1933    2473    4080 
   Size:       1933     540    1607

  Fragments arranged by size: 

                1933    1607     540
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2492    4080 
   Size:       2492    1588
 
BsaAI yAC'GTr

Cuts at:      0     642    4080 
   Size:        642    3438
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     269     299    1446    1476    2398    2428    2834    2864 
   Size:        269      30    1147      30     922      30     406      30

Cuts at:   2864    4080 
   Size:       1216

  Fragments arranged by size: 

                1216    1147     922     406     269      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1164    1542    3373    4080 
   Size:       1164     378    1831     707

  Fragments arranged by size: 

                1831    1164     707     378
 
BseYI C'CCAG_C

Cuts at:      0     157     422    3832    4080 
   Size:        157     265    3410     248

  Fragments arranged by size: 

                3410     265     248     157
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     554    1441    3513    4080 
   Size:        554     887    2072     567

  Fragments arranged by size: 

                2072     887     567     554
 
BsiEI CG_ry'CG

Cuts at:      0      96    4080 
   Size:         96    3984
 
BsiHKAI G_wGCw'C

Cuts at:      0      16    1145    4080 
   Size:         16    1129    2935

  Fragments arranged by size: 

                2935    1129      16
 
Bsp1286I G_dGCh'C

Cuts at:      0      16     170     596    1145    1371    2340    4080 
   Size:         16     154     426     549     226     969    1740

  Fragments arranged by size: 

                1740     969     549     426     226     154      16
 
BspHI T'CATG_A

Cuts at:      0    1098    1352    4080 
   Size:       1098     254    2728

  Fragments arranged by size: 

                2728    1098     254
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2655    4080 
   Size:       2655    1425
 
BsrDI GCAATG_nn'

Cuts at:      0     530    2332    4080 
   Size:        530    1802    1748

  Fragments arranged by size: 

                1802    1748     530
 
BsrGI T'GTAC_A

Cuts at:      0      20     390    4080 
   Size:         20     370    3690

  Fragments arranged by size: 

                3690     370      20
 
BssSI C'ACGA_G

Cuts at:      0    1442    1658    4080 
   Size:       1442     216    2422

  Fragments arranged by size: 

                2422    1442     216
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3913    4080 
   Size:       3913     167
 
BstEII G'GTnAC_C

Cuts at:      0     304    4080 
   Size:        304    3776
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     177    1057    4080 
   Size:        177     880    3023

  Fragments arranged by size: 

                3023     880     177
 
BstYI r'GATC_y

Cuts at:      0     709    1535    2161    2448    4080 
   Size:        709     826     626     287    1632

  Fragments arranged by size: 

                1632     826     709     626     287
 
Bsu36I CC'TnA_GG

Cuts at:      0    1196    4080 
   Size:       1196    2884
 
BtsI GCAGTG_nn'

Cuts at:      0     828     843    2216    3170    4080 
   Size:        828      15    1373     954     910

  Fragments arranged by size: 

                1373     954     910     828      15
 
DraI TTT'AAA

Cuts at:      0     443    4080 
   Size:        443    3637
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1992    4080 
   Size:       1992    2088
 
EaeI y'GGCC_r

Cuts at:      0      71      93    1398    1650    4080 
   Size:         71      22    1305     252    2430

  Fragments arranged by size: 

                2430    1305     252      71      22
 
EagI C'GGCC_G

Cuts at:      0      93    4080 
   Size:         93    3987
 
EarI CTCTTCn'nnn_

Cuts at:      0     602     611     677     895    1128    1853    2491    3350 
   Size:        602       9      66     218     233     725     638     859

Cuts at:   3350    3642    4080 
   Size:        292     438

  Fragments arranged by size: 

                 859     725     638     602     438     292     233     218
                  66       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168    1448    1834    1973    2282    2515    2714    3083 
   Size:        168    1280     386     139     309     233     199     369

Cuts at:   3083    3506    4061    4080 
   Size:        423     555      19

  Fragments arranged by size: 

                1280     555     423     386     369     309     233     199
                 168     139      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      72     128    2662    4080 
   Size:         72      56    2534    1418

  Fragments arranged by size: 

                2534    1418      72      56
 
EcoO109I rG'GnC_Cy

Cuts at:      0      77     116     173    4080 
   Size:         77      39      57    3907

  Fragments arranged by size: 

                3907      77      57      39
 
HaeII r_GCGC'y

Cuts at:      0      49     244    4080 
   Size:         49     195    3836

  Fragments arranged by size: 

                3836     195      49
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     580     612    1143    1175    1236    1268    1446    1478 
   Size:        580      32     531      32      61      32     178      32

Cuts at:   1478    2086    2118    2398    2430    2673    2705    2974    3006 
   Size:        608      32     280      32     243      32     269      32

Cuts at:   3006    3127    3159    4080 
   Size:        121      32     921

  Fragments arranged by size: 

                 921     608     580     531     280     269     243     178
                 121      61      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2012    4028    4080 
   Size:       2012    2016      52

  Fragments arranged by size: 

                2016    2012      52
 
HindIII A'AGCT_T

Cuts at:      0    2440    4080 
   Size:       2440    1640
 
MfeI C'AATT_G

Cuts at:      0    3506    4080 
   Size:       3506     574
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3149    4080 
   Size:       3149     931
 
MscI TGG'CCA

Cuts at:      0    1652    4080 
   Size:       1652    2428
 
MslI CAynn'nnrTG

Cuts at:      0    1055    1313    1658    1954    2398    3601    4080 
   Size:       1055     258     345     296     444    1203     479

  Fragments arranged by size: 

                1203    1055     479     444     345     296     258
 
MspA1I CmG'CkG

Cuts at:      0     106    2934    4080 
   Size:        106    2828    1146

  Fragments arranged by size: 

                2828    1146     106
 
NdeI CA'TA_TG

Cuts at:      0    1665    3797    4080 
   Size:       1665    2132     283

  Fragments arranged by size: 

                2132    1665     283
 
NruI TCG'CGA

Cuts at:      0       6    4080 
   Size:          6    4074
 
NsiI A_TGCA'T

Cuts at:      0    1179    1890    3608    3808    4080 
   Size:       1179     711    1718     200     272

  Fragments arranged by size: 

                1718    1179     711     272     200
 
NspI r_CATG'y

Cuts at:      0    3099    3610    3806    4080 
   Size:       3099     511     196     274

  Fragments arranged by size: 

                3099     511     274     196
 
PfoI T'CCnGG_A

Cuts at:      0    2852    4080 
   Size:       2852    1228
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2834    2866    4080 
   Size:       2834      32    1214

  Fragments arranged by size: 

                2834    1214      32
 
PshAI GACnn'nnGTC

Cuts at:      0    1076    2899    4080 
   Size:       1076    1823    1181

  Fragments arranged by size: 

                1823    1181    1076
 
PspOMI G'GGCC_C

Cuts at:      0     166    1367    4080 
   Size:        166    1201    2713

  Fragments arranged by size: 

                2713    1201     166
 
PstI C_TGCA'G

Cuts at:      0    2681    4080 
   Size:       2681    1399
 
PvuII CAG'CTG

Cuts at:      0    2934    4080 
   Size:       2934    1146
 
SfcI C'TryA_G

Cuts at:      0    1539    1602    2677    2717    2889    3149    3195    4031 
   Size:       1539      63    1075      40     172     260      46     836

Cuts at:   4031    4080 
   Size:         49

  Fragments arranged by size: 

                1539    1075     836     260     172      63      49      46
                  40
 
SmaI CCC'GGG

Cuts at:      0     114    4080 
   Size:        114    3966
 
SmlI C'TyrA_G

Cuts at:      0    1117    1912    2452    2659    4080 
   Size:       1117     795     540     207    1421

  Fragments arranged by size: 

                1421    1117     795     540     207
 
SnaBI TAC'GTA

Cuts at:      0     642    4080 
   Size:        642    3438
 
SphI G_CATG'C

Cuts at:      0    3099    3610    3806    4080 
   Size:       3099     511     196     274

  Fragments arranged by size: 

                3099     511     274     196
 
SspI AAT'ATT

Cuts at:      0    1924    4080 
   Size:       1924    2156
 
StuI AGG'CCT

Cuts at:      0      67     866    1155    4080 
   Size:         67     799     289    2925

  Fragments arranged by size: 

                2925     799     289      67
 
StyI C'CwwG_G

Cuts at:      0     176     200    1553    2132    3789    4080 
   Size:        176      24    1353     579    1657     291

  Fragments arranged by size: 

                1657    1353     579     291     176      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2525    4080 
   Size:       2525    1555
 
TatI w'GTAC_w

Cuts at:      0      20     390     690    1216    1773    3562    4080 
   Size:         20     370     300     526     557    1789     518

  Fragments arranged by size: 

                1789     557     526     518     370     300      20
 
Tth111I GACn'n_nGTC

Cuts at:      0    2146    4080 
   Size:       2146    1934
 
XbaI T'CTAG_A

Cuts at:      0    2373    4080 
   Size:       2373    1707
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     343     419    2302    4080 
   Size:        343      76    1883    1778

  Fragments arranged by size: 

                1883    1778     343      76
 
XmaI C'CCGG_G

Cuts at:      0     112    4080 
   Size:        112    3968
 
XmnI GAAnn'nnTTC

Cuts at:      0    2126    3935    4080 
   Size:       2126    1809     145

  Fragments arranged by size: 

                2126    1809     145
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AhdI     AleI     AlfI     AloI    AlwNI
     ApaI     ApoI     AseI     AvaI    AvrII     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI
    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtsI
     DraI     DrdI     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I   HincII  HindIII     MfeI     MmeI     MscI     MslI
   MspA1I     NdeI     NruI     NsiI     NspI     PfoI     PpiI    PshAI
   PspOMI     PstI    PvuII     SfcI     SmaI     SmlI    SnaBI     SphI
     SspI     StuI     StyI    TaqII     TatI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI    ApaLI
     AscI    AsiSI     BaeI    BamHI     BbeI     BlpI    BmgBI     BmtI
     BplI    BsaBI    BsaHI    BsaWI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrFI   BssHII    BstBI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI   DraIII     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MluI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     PacI     PasI     PciI    PflMI
     PmeI     PmlI    PpuMI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SpeI     SrfI     SwaI     TstI
     XhoI     ZraI
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