Restriction map for pF1KB0983
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HindIII     HpaI        HphI        
KasI        KspI        MaeI        MaeII       MaeIII      
MamI        MflI        MluI        MluNI       MroI        
MscI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp45I      Tth111I     XbaI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        282  GGTACC               G^GTACC
AcsI          114  GAATTT               [GA]^AATT[CT]
AcyI          630  GACGCC               G[GA]^CG[CT]C
AlwI          120  GATCC                GGATC(4/5)
ApaI          597  GGGCCC               GGGCC^C
ApoI          114  GAATTT               [GA]^AATT[CT]
Asp718I       282  GGTACC               G^GTACC
BcnI          652  CCGGG                CC^[GC]GG
BsaHI         630  GACGCC               G[GA]^CG[CT]C
BspMI         417  GCAGGT               ACCTGC(4/8)
BsrDI         273  CATTGC               GCAATG(2/0)
BstAPI        451  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        410  CATCC                GGATG(2/0)
BstXI         236  CCATGACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI          593  CCATGG               C^C[GA][CT]GG
EaeI           69  TGGCCG               [CT]^GGCC[GA]
EarI          437  GAAGAG               CTCTTC(1/4)
EcoT14I       593  CCATGG               C^C[AT][AT]GG
FauI          192  GCGGG                CCCGC(4/6)
FokI          410  CATCC                GGATG(9/13)
HapII         652  CCGG                 C^CGG
Hin1I         630  GACGCC               G[GA]^CG[CT]C
HpaII         652  CCGG                 C^CGG
KpnI          282  GGTACC               GGTAC^C
Ksp632I       437  GAAGAG               CTCTTC(1/4)
MfeI          605  CAATTG               C^AATTG
MseI           92  TTAA                 T^TAA
MslI          237  CATGACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          652  CCGG                 C^CGG
MunI          605  CAATTG               C^AATTG
NciI          652  CCGGG                CC^[GC]GG
NcoI          593  CCATGG               C^CATGG
NdeI          109  CATATG               CA^TATG
NspI          641  GCATGC               [GA]CATG^[CT]
PaeR7I        623  CTCGAG               C^TCGAG
PleI          426  GACTC                GAGTC(4/5)
PstI           28  CTGCAG               CTGCA^G
SexAI         576  ACCTGGT              A^CC[AT]GGT
SfaNI          24  GATGC                GCATC(5/9)
SmlI          623  CTCGAG               C^T[CT][GA]AG
SphI          641  GCATGC               GCATG^C
StyI          593  CCATGG               C^C[AT][AT]GG
TaqI          624  TCGA                 T^CGA
TfiI           74  GAATC                G^A[AT]TC
Tru9I          92  TTAA                 T^TAA
TspRI         444  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       624  TCGA                 T^CGA
XcmI          363  CCAGGCCCCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          623  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI         383  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              452  CAGCTGCTG           
AvaI          278  CTCGGG               C^[CT]CG[GA]G
              623  CTCGAG              
AvaII         406  GGACC                G^G[AT]CC
              685  GGTCC               
BanI           85  GGCACC               G^G[CT][GA]CC
              282  GGTACC              
BanII         488  GAGCCC               G[GA]GC[CT]^C
              597  GGGCCC              
BciVI          56  GTATCC               GTATCC(6/5)
              232  GTATCC              
BsaI          585  GAGACC               GGTCTC(1/5)
              660  GAGACC              
BsmAI         585  GAGAC                GTCTC(1/5)
              660  GAGAC               
BsmFI         405  GGGAC                GGGAC(10/14)
              628  GGGAC               
BsmI          162  GAATGC               GAATGC(1/-1)
              701  GAATGC              
BsoBI         278  CTCGGG               C^[CT]CG[GA]G
              623  CTCGAG              
BsrI          268  ACTGG                ACTGG(1/-1)
              476  CCAGT               
Cac8I         382  GCAGGC               GC[ACGT]^[ACGT]GC
              641  GCATGC              
DpnII         120  GATC                 ^GATC
              150  GATC                
HgaI          630  GACGC                GACGC(5/10)
              705  GCGTC               
HinfI          74  GAATC                G^A[ACGT]TC
              426  GACTC               
MboI          120  GATC                 ^GATC
              150  GATC                
NdeII         120  GATC                 ^GATC
              150  GATC                
PflMI         286  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              601  CCACCAATTGG         
Sau3AI        120  GATC                 ^GATC
              150  GATC                
Van91I        286  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              601  CCACCAATTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
Bsp1286I    BssKI       BstNI       Cfr13I      DraII       
EcoNI       EcoO109I    EcoRII      Fnu4HI      HaeIII      
ItaI        MboII       MnlI        MspA1I      MvaI        
MwoI        NlaIII      NlaIV       PvuII       RsaI        
Sau96I      ScrFI       SfcI        TseI        Tsp509I     
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