Restriction map for pF1KE1415
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaII       AvrII       
BalI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmFI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        HaeII       HapII       HgaI        Hin1I       
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tsp45I      Tsp509I     Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          170  GTCGAC               GT^[AC][GT]AC
AfaI          385  GTAC                 GT^AC
AlwNI          22  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         324  GTGCTC               G[AT]GC[AT]^C
AvaI          145  CCCGAG               C^[CT]CG[GA]G
AviII         505  TGCGCA               TGC^GCA
BamHI         347  GGATCC               G^GATCC
BanI          276  GGCACC               G^G[CT][GA]CC
BfaI           37  CTAG                 C^TAG
BpmI          177  CTGGAG               CTGGAG(16/14)
BseRI         667  GAGGAG               GAGGAG(10/8)
BsiHKAI       324  GTGCTC               G[AT]GC[AT]^C
BsmI          186  GAATGC               GAATGC(1/-1)
BsoBI         145  CCCGAG               C^[CT]CG[GA]G
BsrBI         379  GAGCGG               CCGCTC(-3/-3)
BstYI         347  GGATCC               [GA]^GATC[CT]
EaeI          618  TGGCCG               [CT]^GGCC[GA]
FauI          381  GCGGG                CCCGC(4/6)
FspI          505  TGCGCA               TGC^GCA
HincII        170  GTCGAC               GT[CT]^[GA]AC
HindII        170  GTCGAC               GT[CT]^[GA]AC
MaeI           37  CTAG                 C^TAG
MaeII         392  ACGT                 A^CGT
MflI          347  GGATCC               [GA]^GATC[CT]
MseI          497  TTAA                 T^TAA
PflMI         351  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          579  GACTC                GAGTC(4/5)
PstI          689  CTGCAG               CTGCA^G
PvuII          22  CAGCTG               CAG^CTG
RsaI          385  GTAC                 GT^AC
SalI          170  GTCGAC               G^TCGAC
SfaNI         626  GCATC                GCATC(5/9)
TfiI          248  GATTC                G^A[AT]TC
Tru9I         497  TTAA                 T^TAA
Van91I        351  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          662  CCAATGAGGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         347  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

ApaI          340  GGGCCC               GGGCC^C
              709  GGGCCC              
BanII         340  GGGCCC               G[GA]GC[CT]^C
              709  GGGCCC              
BsrDI         420  GCAATG               GCAATG(2/0)
              566  GCAATG              
CfoI          372  GCGC                 GCG^C
              506  GCGC                
DpnII         348  GATC                 ^GATC
              361  GATC                
HhaI          372  GCGC                 GCG^C
              506  GCGC                
HinP1I        372  GCGC                 G^CGC
              506  GCGC                
HinfI         248  GATTC                G^A[ACGT]TC
              579  GACTC               
MboI          348  GATC                 ^GATC
              361  GATC                
MboII         183  GAAGA                GAAGA(8/7)
              430  GAAGA               
MslI          223  CATAAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              627  CATCCAGATG          
NdeII         348  GATC                 ^GATC
              361  GATC                
Sau3AI        348  GATC                 ^GATC
              361  GATC                
TaqI          171  TCGA                 T^CGA
              641  TCGA                
TthHB8I       171  TCGA                 T^CGA
              641  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BlpI        
BmyI        Bpu1102I    BsaJI       BsiYI       BslI        
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       CelII       Cfr13I      DdeI        
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      ItaI        MnlI        MspA1I      MvaI        
MwoI        NlaIII      NlaIV       Sau96I      ScrFI       
SfcI        TseI        TspRI       
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