Restriction map for pF1KE1415
 (Linear) (Six-base) MAPSORT of: KIEE1415.seq  Check: 4789  from: 1  to: 771



KIEE1415.orf

 With 182 enzymes: SgfI * 

                        August 9, 2016 17:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     288     319     345     460     771 
   Size:        288      31      26     115     311

  Fragments arranged by size: 

                 311     288     115      31      26
 
AccI GT'mk_AC

Cuts at:      0     171     771 
   Size:        171     600
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     473     771 
   Size:        473     298
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     300     771 
   Size:        300     471
 
AlwNI CAG_nnn'CTG

Cuts at:      0      27     771 
   Size:         27     744
 
ApaI G_GGCC'C

Cuts at:      0     344     713     771 
   Size:        344     369      58

  Fragments arranged by size: 

                 369     344      58
 
AvaI C'yCGr_G

Cuts at:      0     145     771 
   Size:        145     626
 
BamHI G'GATC_C

Cuts at:      0     347     771 
   Size:        347     424
 
BanI G'GyrC_C

Cuts at:      0     276     771 
   Size:        276     495
 
BanII G_rGCy'C

Cuts at:      0     344     713     771 
   Size:        344     369      58

  Fragments arranged by size: 

                 369     344      58
 
BlpI GC'TnA_GC

Cuts at:      0       5     736     766     771 
   Size:          5     731      30       5

  Fragments arranged by size: 

                 731      30       5       5
 
Bme1580I G_kGCm'C

Cuts at:      0     344     398     713     771 
   Size:        344      54     315      58

  Fragments arranged by size: 

                 344     315      58      54
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     771 
   Size:        198     573
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     310     340     771 
   Size:        310      30     431

  Fragments arranged by size: 

                 431     310      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     682     771 
   Size:        682      89
 
BseYI C'CCAG_C

Cuts at:      0     645     706     771 
   Size:        645      61      65

  Fragments arranged by size: 

                 645      65      61
 
BsiHKAI G_wGCw'C

Cuts at:      0     328     771 
   Size:        328     443
 
BsmI GAATG_Cn'

Cuts at:      0     192     771 
   Size:        192     579
 
Bsp1286I G_dGCh'C

Cuts at:      0     328     344     398     713     771 
   Size:        328      16      54     315      58

  Fragments arranged by size: 

                 328     315      58      54      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     288     319     345     460     771 
   Size:        288      31      26     115     311

  Fragments arranged by size: 

                 311     288     115      31      26
 
BsrBI CCG'CTC

Cuts at:      0     381     771 
   Size:        381     390
 
BsrDI GCAATG_nn'

Cuts at:      0     427     573     771 
   Size:        427     146     198

  Fragments arranged by size: 

                 427     198     146
 
BstYI r'GATC_y

Cuts at:      0     347     771 
   Size:        347     424
 
BtsI GCAGTG_nn'

Cuts at:      0     172     515     771 
   Size:        172     343     256

  Fragments arranged by size: 

                 343     256     172
 
EaeI y'GGCC_r

Cuts at:      0     618     771 
   Size:        618     153
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     473     771 
   Size:        198     275     298

  Fragments arranged by size: 

                 298     275     198
 
EcoO109I rG'GnC_Cy

Cuts at:      0     142     340     341     710     771 
   Size:        142     198       1     369      61

  Fragments arranged by size: 

                 369     198     142      61       1
 
FspI TGC'GCA

Cuts at:      0     507     771 
   Size:        507     264
 
HincII GTy'rAC

Cuts at:      0     172     771 
   Size:        172     599
 
MslI CAynn'nnrTG

Cuts at:      0     227     631     771 
   Size:        227     404     140

  Fragments arranged by size: 

                 404     227     140
 
MspA1I CmG'CkG

Cuts at:      0       9      24     623     771 
   Size:          9      15     599     148

  Fragments arranged by size: 

                 599     148      15       9
 
PasI CC'CwG_GG

Cuts at:      0     651     713     771 
   Size:        651      62      58

  Fragments arranged by size: 

                 651      62      58
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     357     771 
   Size:        357     414
 
PfoI T'CCnGG_A

Cuts at:      0     302     771 
   Size:        302     469
 
PspOMI G'GGCC_C

Cuts at:      0     340     709     771 
   Size:        340     369      62

  Fragments arranged by size: 

                 369     340      62
 
PstI C_TGCA'G

Cuts at:      0     693     771 
   Size:        693      78
 
PvuII CAG'CTG

Cuts at:      0      24     771 
   Size:         24     747
 
SalI G'TCGA_C

Cuts at:      0     170     771 
   Size:        170     601
 
SfcI C'TryA_G

Cuts at:      0      50     603     689     771 
   Size:         50     553      86      82

  Fragments arranged by size: 

                 553      86      82      50
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      33     567     771 
   Size:         33     534     204

  Fragments arranged by size: 

                 534     204      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     249     281     771 
   Size:        249      32     490

  Fragments arranged by size: 

                 490     249      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     669     771 
   Size:        669     102
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI    AlwNI     ApaI     AvaI    BamHI
     BanI    BanII     BlpI Bme1580I     BpmI    BsaXI    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BstYI     BtsI
     EaeI  Eco57MI EcoO109I     FspI   HincII     MslI   MspA1I     PasI
    PflMI     PfoI   PspOMI     PstI    PvuII     SalI     SfcI    TaqII
     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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