Restriction map for pF1KE1303
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BciVI       
BfrI        BglI        BlnI        BsaAI       BsaBI       
BsgI        BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
ClaI        CpoI        DrdI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MunI        NarI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
Van91I      XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          748  GTCTAC               GT^[AC][GT]AC
AccII          94  CGCG                 CG^CG
AccIII       1220  TCCGGA               T^CCGGA
AfaI         1086  GTAC                 GT^AC
AhdI         1135  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I       792  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1135  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          480  TGGCCA               TGG^CCA
BanI           32  GGTGCC               G^G[CT][GA]CC
BclI          173  TGATCA               T^GATCA
BcnI          524  CCGGG                CC^[GC]GG
BglII         180  AGATCT               A^GATCT
BlpI           87  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       87  GCTGAGC              GC^T[ACGT]AGC
BsaI         1093  GAGACC               GGTCTC(1/5)
BseAI        1220  TCCGGA               T^CCGGA
BseRI         582  CTCCTC               GAGGAG(10/8)
BsiEI         776  CGGTCG               CG[GA][CT]^CG
BspEI        1220  TCCGGA               T^CCGGA
BsrFI          35  GCCGGC               [GA]^CCGG[CT]
BstUI          94  CGCG                 CG^CG
CelII          87  GCTGAGC              GC^T[ACGT]AGC
CfoI          658  GCGC                 GCG^C
Cfr10I         35  GCCGGC               [GA]^CCGG[CT]
DraI         1237  TTTAAA               TTT^AAA
DraIII       1010  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          480  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1135  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I       879  CCATGG               C^C[AT][AT]GG
FbaI          173  TGATCA               T^GATCA
HhaI          658  GCGC                 GCG^C
HinP1I        658  GCGC                 G^CGC
MaeII        1088  ACGT                 A^CGT
MluNI         480  TGGCCA               TGG^CCA
MroI         1220  TCCGGA               T^CCGGA
MscI          480  TGGCCA               TGG^CCA
MslI         1020  CATCTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           94  CGCG                 CG^CG
NaeI           35  GCCGGC               GCC^GGC
NciI          524  CCGGG                CC^[GC]GG
NcoI          879  CCATGG               C^CATGG
NdeI          112  CATATG               CA^TATG
NgoMIV         35  GCCGGC               G^CCGGC
NspI          248  ACATGC               [GA]CATG^[CT]
PaeR7I        191  CTCGAG               C^TCGAG
RsaI         1086  GTAC                 GT^AC
StuI          854  AGGCCT               AGG^CCT
StyI          879  CCATGG               C^C[AT][AT]GG
TfiI          342  GATTC                G^A[AT]TC
Tsp45I       1016  GTGAC                ^GT[GC]AC
Tsp509I      1213  AATT                 ^AATT
XcmI          852  CCAGGCCTGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          191  CTCGAG               C^TCGAG
XmnI          792  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI         137  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              740  CAGGGGCTG           
AvaI          191  CTCGAG               C^[CT]CG[GA]G
              934  CCCGAG              
BbsI          910  GAAGAC               GAAGAC(2/6)
             1132  GAAGAC              
BfaI          307  CTAG                 C^TAG
              998  CTAG                
BpuAI         910  GAAGAC               GAAGAC(2/6)
             1132  GAAGAC              
BsaWI         366  ACCGGA               [AT]^CCGG[AT]
             1220  TCCGGA              
BsmAI         187  GAGAC                GTCTC(1/5)
             1093  GAGAC               
BsoBI         191  CTCGAG               C^[CT]CG[GA]G
              934  CCCGAG              
BstYI         145  GGATCT               [GA]^GATC[CT]
              180  AGATCT              
DsaI          669  CCACGG               C^C[GA][CT]GG
              879  CCATGG              
EarI          206  CTCTTC               CTCTTC(1/4)
               52  GAAGAG              
Ksp632I       206  CTCTTC               CTCTTC(1/4)
               52  GAAGAG              
MaeI          307  CTAG                 C^TAG
              998  CTAG                
MaeIII        929  GTAAC                ^GT[ACGT]AC
             1016  GTGAC               
MflI          145  GGATCT               [GA]^GATC[CT]
              180  AGATCT              
MseI         1211  TTAA                 T^TAA
             1238  TTAA                
PpuMI         970  AGGACCT              [GA]G^G[AT]CC[CT]
             1064  GGGACCC             
SapI           52  GAAGAGC              GCTCTTC(1/4)
              205  GCTCTTC             
SmlI          191  CTCGAG               C^T[CT][GA]AG
              424  CTTGAG              
Tru9I        1211  TTAA                 T^TAA
             1238  TTAA                
XhoII         145  GGATCT               [GA]^GATC[CT]
              180  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AspHI       
AvaII       BanII       BbvI        BcgI        BmyI        
BpmI        BsaHI       BsaJI       BsiHKAI     BsiYI       
BslI        BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstXI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        Hin1I       HinfI       HpaII       
HphI        ItaI        MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TseI        TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com