Restriction map for pF1KE1303
 (Linear) (Six-base) MAPSORT of: KIEE1303.seq  Check: 501  from: 1  to: 1248



KIEE1303.orf

 With 182 enzymes: SgfI * 

                        May 19, 2017 16:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      51     737    1248 
   Size:         51     686     511

  Fragments arranged by size: 

                 686     511      51
 
AccI GT'mk_AC

Cuts at:      0     749    1248 
   Size:        749     499
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     929    1151    1248 
   Size:        929     222      97

  Fragments arranged by size: 

                 929     222      97
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1140    1248 
   Size:       1140     108
 
AlwNI CAG_nnn'CTG

Cuts at:      0     142     745    1248 
   Size:        142     603     503

  Fragments arranged by size: 

                 603     503     142
 
AvaI C'yCGr_G

Cuts at:      0     191     934    1248 
   Size:        191     743     314

  Fragments arranged by size: 

                 743     314     191
 
BanI G'GyrC_C

Cuts at:      0      32    1248 
   Size:         32    1216
 
BanII G_rGCy'C

Cuts at:      0     476     530    1125    1248 
   Size:        476      54     595     123

  Fragments arranged by size: 

                 595     476     123      54
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     917    1139    1248 
   Size:        917     222     109

  Fragments arranged by size: 

                 917     222     109
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     275     309     604     638     925     959    1248 
   Size:        275      34     295      34     287      34     289

  Fragments arranged by size: 

                 295     289     287     275      34      34      34
 
BclI T'GATC_A

Cuts at:      0     173    1248 
   Size:        173    1075
 
BglII A'GATC_T

Cuts at:      0     180    1248 
   Size:        180    1068
 
BlpI GC'TnA_GC

Cuts at:      0      88    1248 
   Size:         88    1160
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     316    1007    1248 
   Size:        316     691     241

  Fragments arranged by size: 

                 691     316     241
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     221     912    1248 
   Size:        221     691     336

  Fragments arranged by size: 

                 691     336     221
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     419     670    1080    1248 
   Size:        205     214     251     410     168

  Fragments arranged by size: 

                 410     251     214     205     168
 
Bpu10I CC'TnA_GC

Cuts at:      0     463    1153    1248 
   Size:        463     690      95

  Fragments arranged by size: 

                 690     463      95
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445    1248 
   Size:        445     803
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1087    1248 
   Size:       1087     161
 
BsaHI Gr'CG_yC

Cuts at:      0      80     573     989    1248 
   Size:         80     493     416     259

  Fragments arranged by size: 

                 493     416     259      80
 
BsaWI w'CCGG_w

Cuts at:      0     366    1220    1248 
   Size:        366     854      28

  Fragments arranged by size: 

                 854     366      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     573    1248 
   Size:        573     675
 
BseYI C'CCAG_C

Cuts at:      0     817    1248 
   Size:        817     431
 
BsiEI CG_ry'CG

Cuts at:      0     779    1248 
   Size:        779     469
 
BsiHKAI G_wGCw'C

Cuts at:      0     332     423     678    1248 
   Size:        332      91     255     570

  Fragments arranged by size: 

                 570     332     255      91
 
Bsp1286I G_dGCh'C

Cuts at:      0     332     423     476     530     678    1125    1248 
   Size:        332      91      53      54     148     447     123

  Fragments arranged by size: 

                 447     332     148     123      91      54      53
 
BspEI T'CCGG_A

Cuts at:      0    1220    1248 
   Size:       1220      28
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      51     737    1145    1248 
   Size:         51     686     408     103

  Fragments arranged by size: 

                 686     408     103      51
 
BsrFI r'CCGG_y

Cuts at:      0      35    1248 
   Size:         35    1213
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     384     633     859    1248 
   Size:        384     249     226     389

  Fragments arranged by size: 

                 389     384     249     226
 
BstYI r'GATC_y

Cuts at:      0     145     180    1248 
   Size:        145      35    1068

  Fragments arranged by size: 

                1068     145      35
 
BtgI C'CryG_G

Cuts at:      0     669     879    1248 
   Size:        669     210     369

  Fragments arranged by size: 

                 669     369     210
 
BtsI GCAGTG_nn'

Cuts at:      0     483    1123    1248 
   Size:        483     640     125

  Fragments arranged by size: 

                 640     483     125
 
DraI TTT'AAA

Cuts at:      0    1239    1248 
   Size:       1239       9
 
DraIII CAC_nnn'GTG

Cuts at:      0    1015    1248 
   Size:       1015     233
 
EaeI y'GGCC_r

Cuts at:      0     480    1248 
   Size:        480     768
 
EarI CTCTTCn'nnn_

Cuts at:      0      47     212    1248 
   Size:         47     165    1036

  Fragments arranged by size: 

                1036     165      47
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     419     670     929    1080    1151    1248 
   Size:        205     214     251     259     151      71      97

  Fragments arranged by size: 

                 259     251     214     205     151      97      71
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     971    1065    1248 
   Size:        107     864      94     183

  Fragments arranged by size: 

                 864     183     107      94
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     165     197     979    1011    1126    1158    1248 
   Size:        165      32     782      32     115      32      90

  Fragments arranged by size: 

                 782     165     115      90      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     572    1248 
   Size:        572     676
 
MscI TGG'CCA

Cuts at:      0     482    1248 
   Size:        482     766
 
MslI CAynn'nnrTG

Cuts at:      0    1024    1248 
   Size:       1024     224
 
MspA1I CmG'CkG

Cuts at:      0     132     171     812     984    1248 
   Size:        132      39     641     172     264

  Fragments arranged by size: 

                 641     264     172     132      39
 
NaeI GCC'GGC

Cuts at:      0      37    1248 
   Size:         37    1211
 
NcoI C'CATG_G

Cuts at:      0     879    1248 
   Size:        879     369
 
NdeI CA'TA_TG

Cuts at:      0     113    1248 
   Size:        113    1135
 
NgoMIV G'CCGG_C

Cuts at:      0      35    1248 
   Size:         35    1213
 
NspI r_CATG'y

Cuts at:      0     252    1248 
   Size:        252     996
 
PasI CC'CwG_GG

Cuts at:      0     739    1248 
   Size:        739     509
 
PfoI T'CCnGG_A

Cuts at:      0     956    1057    1248 
   Size:        956     101     191

  Fragments arranged by size: 

                 956     191     101
 
PpuMI rG'GwC_Cy

Cuts at:      0     971    1065    1248 
   Size:        971      94     183

  Fragments arranged by size: 

                 971     183      94
 
PsiI TTA'TAA

Cuts at:      0    1190    1248 
   Size:       1190      58
 
PspXI vC'TCGA_Gb

Cuts at:      0     191    1248 
   Size:        191    1057
 
PstI C_TGCA'G

Cuts at:      0      62     734    1142    1248 
   Size:         62     672     408     106

  Fragments arranged by size: 

                 672     408     106      62
 
PvuII CAG'CTG

Cuts at:      0     171     812     984    1248 
   Size:        171     641     172     264

  Fragments arranged by size: 

                 641     264     172     171
 
SanDI GG'GwC_CC

Cuts at:      0    1065    1248 
   Size:       1065     183
 
SapI GCTCTTCn'nnn_

Cuts at:      0      47     212    1248 
   Size:         47     165    1036

  Fragments arranged by size: 

                1036     165      47
 
SfcI C'TryA_G

Cuts at:      0      58     730    1138    1248 
   Size:         58     672     408     110

  Fragments arranged by size: 

                 672     408     110      58
 
SmlI C'TyrA_G

Cuts at:      0     191     424    1248 
   Size:        191     233     824

  Fragments arranged by size: 

                 824     233     191
 
StuI AGG'CCT

Cuts at:      0     856    1248 
   Size:        856     392
 
StyI C'CwwG_G

Cuts at:      0     879    1248 
   Size:        879     369
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     390     765    1026    1248 
   Size:        390     375     261     222

  Fragments arranged by size: 

                 390     375     261     222
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     755     787    1248 
   Size:        755      32     461

  Fragments arranged by size: 

                 755     461      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     859    1248 
   Size:        859     389
 
XhoI C'TCGA_G

Cuts at:      0     191    1248 
   Size:        191    1057
 
XmnI GAAnn'nnTTC

Cuts at:      0     796    1248 
   Size:        796     452
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI    AlwNI     AvaI     BanI    BanII
     BbsI     BcgI     BclI    BglII     BlpI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspMI    BsrFI    BstXI    BstYI     BtgI
     BtsI     DraI   DraIII     EaeI     EarI  Eco57MI EcoO109I    Hin4I
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NspI     PasI     PfoI    PpuMI     PsiI    PspXI     PstI    PvuII
    SanDI     SapI     SfcI     SmlI     StuI     StyI    TaqII     TstI
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI    BfrBI     BglI
 Bme1580I    BmgBI     BmtI    BsaAI    BsaBI    BsaXI     BsgI    BsiWI
     BsmI    BsmBI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI   PspOMI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XmaI
     ZraI
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