Restriction map for pF1KB5403
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       AclI        AflII       ApaI        AscI        
Asp700I     AvrII       BalI        BbrPI       BbvCI       
BciVI       BfrI        BglII       BlnI        BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco52I      
EcoO65I     EcoRI       EcoT22I     FseI        HincII      
HindII      HindIII     HpaI        MfeI        MluI        
MluNI       MscI        MunI        NaeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SalI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SspBI       SwaI        
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

Acc65I       1745  GGTACC               G^GTACC
AccI         2053  GTCTAC               GT^[AC][GT]AC
AccIII        191  TCCGGA               T^CCGGA
AcsI         1360  GAATTT               [GA]^AATT[CT]
AgeI           19  ACCGGT               A^CCGGT
Alw44I       2452  GTGCAC               G^TGCAC
AocI         1576  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1460  AGCGCT               AGC^GCT
ApaLI        2452  GTGCAC               G^TGCAC
ApoI         1360  GAATTT               [GA]^AATT[CT]
AseI         2479  ATTAAT               AT^TAAT
AsnI         2479  ATTAAT               AT^TAAT
Asp718I      1745  GGTACC               G^GTACC
BamHI        1688  GGATCC               G^GATCC
BbeI         2000  GGCGCC               GGCGC^C
BcgI          489  CGAGCAGTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          337  CTAG                 C^TAG
BlpI          111  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      111  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1405  TACGTG               [CT]AC^GT[GA]
BsaBI        1224  GATGGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2657  GAGACC               GGTCTC(1/5)
BseAI         191  TCCGGA               T^CCGGA
BsmBI        2413  GAGACG               CGTCTC(1/5)
BspEI         191  TCCGGA               T^CCGGA
BspHI        1769  TCATGA               T^CATGA
BsrBI        1996  GAGCGG               CCGCTC(-3/-3)
BsrFI          19  ACCGGT               [GA]^CCGG[CT]
BssSI        1016  CACGAG               CACGAG(-5/-1)
Bsu36I       1576  CCTGAGG              CC^T[ACGT]AGG
CelII         111  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         19  ACCGGT               [GA]^CCGG[CT]
Eco47III     1460  AGCGCT               AGC^GCT
Eco81I       1576  CCTGAGG              CC^T[ACGT]AGG
EcoRV        1228  GATATC               GAT^ATC
KasI         2000  GGCGCC               G^GCGCC
KpnI         1745  GGTACC               GGTAC^C
KspI         2700  CCGCGG               CCGC^GG
MaeI          337  CTAG                 C^TAG
MaeII        1406  ACGT                 A^CGT
MamI         1224  GATGGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          191  TCCGGA               T^CCGGA
NarI         2000  GGCGCC               GG^CGCC
NcoI         1835  CCATGG               C^CATGG
NdeI          266  CATATG               CA^TATG
PinAI          19  ACCGGT               A^CCGGT
PshBI        2479  ATTAAT               AT^TAAT
RcaI         1769  TCATGA               T^CATGA
SacII        2700  CCGCGG               CCGC^GG
SapI          539  GCTCTTC              GCTCTTC(1/4)
SbfI         1962  CCTGCAGG             CCTGCA^GG
SexAI        2057  ACCTGGT              A^CC[AT]GGT
SfoI         2000  GGCGCC               GGC^GCC
SmlI          759  CTCAAG               C^T[CT][GA]AG
SphI         1797  GCATGC               GCATG^C
Sse8387I     1962  CCTGCAGG             CCTGCA^GG
SspI         1267  AATATT               AAT^ATT
StuI         1869  AGGCCT               AGG^CCT
Tsp509I      1361  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcyI         1105  GGCGTC               G[GA]^CG[CT]C
             2000  GGCGCC              
AhdI           28  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1903  GACATCCAGTC         
AspEI          28  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1903  GACATCCAGTC         
AvaI          487  CCCGAG               C^[CT]CG[GA]G
             2308  CCCGAG              
AviII        1514  TGCGCA               TGC^GCA
             1955  TGCGCA              
BbsI          685  GAAGAC               GAAGAC(2/6)
             2247  GAAGAC              
BclI         2126  TGATCA               T^GATCA
             2474  TGATCA              
BglI         2218  GCCTCCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2699  GCCGCGGTGGC         
BpuAI         685  GAAGAC               GAAGAC(2/6)
             2247  GAAGAC              
BsaHI        1105  GGCGTC               G[GA]^CG[CT]C
             2000  GGCGCC              
BsoBI         487  CCCGAG               C^[CT]CG[GA]G
             2308  CCCGAG              
DraIII        228  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1310  CACTGCGTG           
Eam1105I       28  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1903  GACATCCAGTC         
FbaI         2126  TGATCA               T^GATCA
             2474  TGATCA              
FspI         1514  TGCGCA               TGC^GCA
             1955  TGCGCA              
Hin1I        1105  GGCGTC               G[GA]^CG[CT]C
             2000  GGCGCC              
PstI         1963  CTGCAG               CTGCA^G
             2569  CTGCAG              
SacI           61  GAGCTC               GAGCT^C
              250  GAGCTC              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AlwNI       AspHI       AspI        AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspLU11I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflFI       
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
TspRI       Tth111I     TthHB8I     XcmI        XhoII       
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