Restriction map for pF1KE1004
##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaJI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DpnII       DraI        DraII       DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HindIII     HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AspI          382  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcnI          206  CCGGG                CC^[GC]GG
BpmI           29  CTGGAG               CTGGAG(16/14)
BsaI           32  GAGACC               GGTCTC(1/5)
BseRI         289  GAGGAG               GAGGAG(10/8)
BsgI          137  GTGCAG               GTGCAG(16/14)
BsmAI          32  GAGAC                GTCTC(1/5)
BstF5I        276  CATCC                GGATG(2/0)
DdeI           23  CTAAG                C^T[ACGT]AG
DraIII        343  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          203  TGGCCG               [CT]^GGCC[GA]
FokI          276  CATCC                GGATG(9/13)
HapII         206  CCGG                 C^CGG
HincII        226  GTCAAC               GT[CT]^[GA]AC
HindII        226  GTCAAC               GT[CT]^[GA]AC
HpaII         206  CCGG                 C^CGG
MboII         180  GAAGA                GAAGA(8/7)
MspI          206  CCGG                 C^CGG
NciI          206  CCGGG                CC^[GC]GG
NlaIV         108  GGCCCC               GG[ACGT]^[ACGT]CC
PflFI         382  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          264  CTGCAG               CTGCA^G
ScaI          248  AGTACT               AGT^ACT
SmlI          462  CTCAAG               C^T[CT][GA]AG
Tsp509I       188  AATT                 ^AATT
Tth111I       382  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          219  GTAC                 GT^AC
              249  GTAC                
AlwNI          63  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
              128  CAGGAGCTG           
AspHI         310  GTGCTC               G[AT]GC[AT]^C
              340  GAGCAC              
BmyI          310  GTGCTC               G[AGT]GC[ACT]^C
              340  GAGCAC              
BsiHKAI       310  GTGCTC               G[AT]GC[AT]^C
              340  GAGCAC              
BsiYI          22  CCTAAGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              279  CCAGCGGGCGG         
BslI           22  CCTAAGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              279  CCAGCGGGCGG         
Bsp1286I      310  GTGCTC               G[AGT]GC[ACT]^C
              340  GAGCAC              
BstAPI        303  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              409  GCAAGCGTTGC         
CfoI            7  GCGC                 GCG^C
              360  GCGC                
Cfr13I         71  GGGCC                G^G[ACGT]CC
              108  GGCCC               
FauI          374  CCCGC                CCCGC(4/6)
              282  GCGGG               
HhaI            7  GCGC                 GCG^C
              360  GCGC                
HinP1I          7  GCGC                 G^CGC
              360  GCGC                
HinfI         368  GAGTC                G^A[ACGT]TC
              452  GAGTC               
MaeIII        212  GTGAC                ^GT[ACGT]AC
              380  GTGAC               
MspA1I        256  CCGCTG               C[AC]G^C[GT]G
              280  CAGCGG              
PleI          368  GAGTC                GAGTC(4/5)
              452  GAGTC               
RsaI          219  GTAC                 GT^AC
              249  GTAC                
Sau96I         71  GGGCC                G^G[ACGT]CC
              108  GGCCC               
SexAI         221  ACCAGGT              A^CC[AT]GGT
              383  ACCAGGT             
SfcI           60  CTACAG               C^T[GA][CT]AG
              264  CTGCAG              
Tsp45I        212  GTGAC                ^GT[GC]AC
              380  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AciI        AluI        BbvI        BsrI        BssKI       
BstNI       Cac8I       EcoRII      Fnu4HI      HaeIII      
ItaI        MnlI        MvaI        MwoI        NlaIII      
ScrFI       TseI        TspRI       
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