################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvrII BamHI BbrPI BbvCI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 77 CGCG CG^CG AccIII 513 TCCGGA T^CCGGA AcyI 878 GGCGCC G[GA]^CG[CT]C AocI 39 CCTAAGG CC^T[ACGT]AGG AviII 788 TGCGCA TGC^GCA BalI 836 TGGCCA TGG^CCA BbeI 878 GGCGCC GGCGC^C BbsI 189 GAAGAC GAAGAC(2/6) BciVI 3 GGATAC GTATCC(6/5) BpuAI 189 GAAGAC GAAGAC(2/6) BsaHI 878 GGCGCC G[GA]^CG[CT]C BsaI 137 GGTCTC GGTCTC(1/5) BsaWI 513 TCCGGA [AT]^CCGG[AT] BseAI 513 TCCGGA T^CCGGA BspEI 513 TCCGGA T^CCGGA BstF5I 653 CATCC GGATG(2/0) BstUI 77 CGCG CG^CG Bsu36I 39 CCTAAGG CC^T[ACGT]AGG CpoI 510 CGGTCCG CG^G[AT]CCG DraIII 792 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 836 TGGCCA [CT]^GGCC[GA] Eco81I 39 CCTAAGG CC^T[ACGT]AGG EcoNI 858 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 653 CATCC GGATG(9/13) FspI 788 TGCGCA TGC^GCA Hin1I 878 GGCGCC G[GA]^CG[CT]C KasI 878 GGCGCC G^GCGCC MluNI 836 TGGCCA TGG^CCA MroI 513 TCCGGA T^CCGGA MscI 836 TGGCCA TGG^CCA MseI 13 TTAA T^TAA MslI 371 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 77 CGCG CG^CG NaeI 1065 GCCGGC GCC^GGC NarI 878 GGCGCC GG^CGCC NgoMIV 1065 GCCGGC G^CCGGC NlaIII 577 CATG CATG^ PpuMI 169 GGGTCCC [GA]G^G[AT]CC[CT] RsrII 510 CGGTCCG CG^G[AT]CCG ScaI 396 AGTACT AGT^ACT SfoI 878 GGCGCC GGC^GCC SmlI 982 CTCAAG C^T[CT][GA]AG StuI 36 AGGCCT AGG^CCT Tru9I 13 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 324 GTAC GT^AC 397 GTAC ApaI 89 GGGCCC GGGCC^C 443 GGGCCC Asp700I 641 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 753 GAAAAGATTC BsgI 151 GTGCAG GTGCAG(16/14) 224 GTGCAG BsmBI 84 GAGACG CGTCTC(1/5) 607 GAGACG BsrBI 744 CCGCTC CCGCTC(-3/-3) 937 GAGCGG BsrFI 465 GCCGGT [GA]^CCGG[CT] 1065 GCCGGC BsrI 399 ACTGG ACTGG(1/-1) 534 ACTGG Cfr10I 465 GCCGGT [GA]^CCGG[CT] 1065 GCCGGC EcoT14I 243 CCAAGG C^C[AT][AT]GG 839 CCAAGG HaeII 878 GGCGCC [GA]GCGC^[CT] 899 AGCGCC PstI 214 CTGCAG CTGCA^G 1042 CTGCAG PvuII 599 CAGCTG CAG^CTG 1003 CAGCTG RsaI 324 GTAC GT^AC 397 GTAC SfaNI 768 GATGC GCATC(5/9) 453 GCATC SfcI 214 CTGCAG C^T[GA][CT]AG 1042 CTGCAG StyI 243 CCAAGG C^C[AT][AT]GG 839 CCAAGG TaqI 104 TCGA T^CGA 109 TCGA TthHB8I 104 TCGA T^CGA 109 TCGA XmnI 641 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 753 GAAAAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AluI AlwI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI SmaI TfiI TseI Tsp45I XmaCI XmaI