Restriction map for
pF1KE2488
(Circular) (Six-base) MAPSORT of: pF1KE2488.seq Check: 8125 from: 1 to: 4193
pF1KE2488 4193 bp
With 182 enzymes: SgfI *
October 10, 2013 10:36 ..
AccI GT'mk_AC
Cuts at: 1231 1231
Size: 4193
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 4033 4033
Size: 4193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 2018 2450 3197 3465 865
Size: 1153 432 747 268 1593
Fragments arranged by size:
1593 1153 747 432 268
AflIII A'CryG_T
Cuts at: 1545 2649 3626 1545
Size: 1104 977 2112
Fragments arranged by size:
2112 1104 977
AgeI A'CCGG_T
Cuts at: 2581 2581
Size: 4193
AleI CACnn'nnGTG
Cuts at: 483 483
Size: 4193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 347 347
Size: 4193
AlwNI CAG_nnn'CTG
Cuts at: 1498 3065 1498
Size: 1567 2626
ApaI G_GGCC'C
Cuts at: 201 555 201
Size: 354 3839
ApaLI G'TGCA_C
Cuts at: 2963 2963
Size: 4193
ApoI r'AATT_y
Cuts at: 1197 3546 3802 1197
Size: 2349 256 1588
Fragments arranged by size:
2349 1588 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 163 241 546 674 1213 163
Size: 78 305 128 539 3143
Fragments arranged by size:
3143 539 305 128 78
BamHI G'GATC_C
Cuts at: 1218 1218
Size: 4193
BanI G'GyrC_C
Cuts at: 477 660 986 1209 1623 1871 1906 477
Size: 183 326 223 414 248 35 2764
Fragments arranged by size:
2764 414 326 248 223 183 35
BanII G_rGCy'C
Cuts at: 201 242 555 629 1207 2237 201
Size: 41 313 74 578 1030 2157
Fragments arranged by size:
2157 1030 578 313 74 41
BbeI G_GCGC'C
Cuts at: 990 1627 1875 990
Size: 637 248 3308
Fragments arranged by size:
3308 637 248
BbsI GAAGACnn'nnnn_
Cuts at: 304 304
Size: 4193
BciVI GTATCCnnnnn_n'
Cuts at: 105 2091 2852 4193 105
Size: 1986 761 1341 105
Fragments arranged by size:
1986 1341 761 105
BclI T'GATC_A
Cuts at: 1713 1713
Size: 4193
BglII A'GATC_T
Cuts at: 1708 3745 1708
Size: 2037 2156
BlpI GC'TnA_GC
Cuts at: 1305 1305
Size: 4193
Bme1580I G_kGCm'C
Cuts at: 201 555 663 1818 1911 2967 201
Size: 354 108 1155 93 1056 1427
Fragments arranged by size:
1427 1155 1056 354 108 93
BmrI ACTGGGnnnn_n'
Cuts at: 1405 1486 1821 3965 1405
Size: 81 335 2144 1633
Fragments arranged by size:
2144 1633 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 664 689 988 1057 427
Size: 237 25 299 69 3563
Fragments arranged by size:
3563 299 237 69 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1369 2248 2740 3038 1075
Size: 294 879 492 298 2230
Fragments arranged by size:
2230 879 492 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 251 3897 251
Size: 3646 547
BsaAI yAC'GTr
Cuts at: 1546 1546
Size: 4193
BsaBI GATnn'nnATC
Cuts at: 6 1707 3744 6
Size: 1701 2037 455
Fragments arranged by size:
2037 1701 455
BsaHI Gr'CG_yC
Cuts at: 987 1624 1872 2574 4070 987
Size: 637 248 702 1496 1110
Fragments arranged by size:
1496 1110 702 637 248
BsaWI w'CCGG_w
Cuts at: 621 1382 1903 2581 2855 3002 3413 621
Size: 761 521 678 274 147 411 1401
Fragments arranged by size:
1401 761 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 259 199
Size: 30 30 4133
Fragments arranged by size:
4133 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 239 487 550 811 985 988 239
Size: 248 63 261 174 3 3444
Fragments arranged by size:
3444 261 248 174 63 3
BseYI C'CCAG_C
Cuts at: 138 383 557 709 771 1166 1618 2376 2953
Size: 245 174 152 62 395 452 758 577
Cuts at: 2953 3449 138
Size: 496 882
Fragments arranged by size:
882 758 577 496 452 395 245 174
152 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 353 280
Size: 73 4120
BsiEI CG_ry'CG
Cuts at: 104 1735 1781 2989 104
Size: 1631 46 1208 1308
Fragments arranged by size:
1631 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1207 1985 2175 2967 1207
Size: 778 190 792 2433
Fragments arranged by size:
2433 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 186 709 186
Size: 523 3670
Bsp1286I G_dGCh'C
Cuts at: 201 242 555 629 663 1207 1818 1911 1985
Size: 41 313 74 34 544 611 93 74
Cuts at: 1985 2175 2237 2967 201
Size: 190 62 730 1427
Fragments arranged by size:
1427 730 611 544 313 190 93 74
74 62 41 34
BspEI T'CCGG_A
Cuts at: 621 3413 621
Size: 2792 1401
BspHI T'CATG_A
Cuts at: 3369 3369
Size: 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1243 1457 1759 2140 1243
Size: 214 302 381 3296
Fragments arranged by size:
3296 381 302 214
BsrBI CCG'CTC
Cuts at: 50 854 1047 2485 2539 4023 4188 50
Size: 804 193 1438 54 1484 165 55
Fragments arranged by size:
1484 1438 804 193 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2105 2105
Size: 4193
BsrFI r'CCGG_y
Cuts at: 573 1173 2191 2372 2581 573
Size: 600 1018 181 209 2185
Fragments arranged by size:
2185 1018 600 209 181
BssSI C'ACGA_G
Cuts at: 2464 2822 2464
Size: 358 3835
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 1570
Size: 4193
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstYI r'GATC_y
Cuts at: 1 1218 1685 1708 2043 2289 3301 3387 3399
Size: 1217 467 23 335 246 1012 86 12
Cuts at: 3399 3745 1
Size: 346 449
Fragments arranged by size:
1217 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 148 148
Size: 4193
BtgI C'CryG_G
Cuts at: 107 107
Size: 4193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2143 2324 2487 2143
Size: 181 163 3849
Fragments arranged by size:
3849 181 163
BtsI GCAGTG_nn'
Cuts at: 1436 1436
Size: 4193
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DraIII CAC_nnn'GTG
Cuts at: 905 905
Size: 4193
DrdI GACnn_nn'nnGTC
Cuts at: 1899 2757 1899
Size: 858 3335
EaeI y'GGCC_r
Cuts at: 944 1778 1952 2343 2370 3715 944
Size: 834 174 391 27 1345 1422
Fragments arranged by size:
1422 1345 834 391 174 27
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2401 2711 2857 4003 2401
Size: 310 146 1146 2591
Fragments arranged by size:
2591 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 664 689 865 988 1057 2018 2450 3197
Size: 237 25 176 123 69 961 432 747
Cuts at: 3197 3465 427
Size: 268 1155
Fragments arranged by size:
1155 961 747 432 268 237 176 123
69 25
EcoNI CCTnn'n_nnAGG
Cuts at: 970 970
Size: 4193
EcoO109I rG'GnC_Cy
Cuts at: 198 278 551 552 768 1332 198
Size: 80 273 1 216 564 3059
Fragments arranged by size:
3059 564 273 216 80 1
EcoRI G'AATT_C
Cuts at: 1197 1197
Size: 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 817 2212 817
Size: 1395 2798
FspI TGC'GCA
Cuts at: 898 1974 898
Size: 1076 3117
HaeII r_GCGC'y
Cuts at: 990 1011 1627 1875 2897 3876 990
Size: 21 616 248 1022 979 1307
Fragments arranged by size:
1307 1022 979 616 248 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 229 231 261 2035 2065 2067 2097 199
Size: 30 2 30 1774 30 2 30 2295
Fragments arranged by size:
2295 1774 30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 1232 1232
Size: 4193
KasI G'GCGC_C
Cuts at: 986 1623 1871 986
Size: 637 248 3308
Fragments arranged by size:
3308 637 248
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2864 3048 2864
Size: 184 4009
MscI TGG'CCA
Cuts at: 946 1954 946
Size: 1008 3185
MslI CAynn'nnrTG
Cuts at: 483 2182 2309 483
Size: 1699 127 2367
Fragments arranged by size:
2367 1699 127
MspA1I CmG'CkG
Cuts at: 264 709 912 1113 1304 1618 1978 2991 3236
Size: 445 203 201 191 314 360 1013 245
Cuts at: 3236 264
Size: 1221
Fragments arranged by size:
1221 1013 445 360 314 245 203 201
191
NaeI GCC'GGC
Cuts at: 1175 2374 1175
Size: 1199 2994
NarI GG'CG_CC
Cuts at: 987 1624 1872 987
Size: 637 248 3308
Fragments arranged by size:
3308 637 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4193
NgoMIV G'CCGG_C
Cuts at: 1173 2372 1173
Size: 1199 2994
NspI r_CATG'y
Cuts at: 1246 1746 2653 1246
Size: 500 907 2786
Fragments arranged by size:
2786 907 500
PasI CC'CwG_GG
Cuts at: 656 656
Size: 4193
PciI A'CATG_T
Cuts at: 2649 2649
Size: 4193
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 4161
PpuMI rG'GwC_Cy
Cuts at: 278 278
Size: 4193
PspOMI G'GGCC_C
Cuts at: 197 551 197
Size: 354 3839
PstI C_TGCA'G
Cuts at: 326 1154 1240 1925 326
Size: 828 86 685 2594
Fragments arranged by size:
2594 828 685 86
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 709 1113 1618 1978 709
Size: 404 505 360 2924
Fragments arranged by size:
2924 505 404 360
RsrII CG'GwC_CG
Cuts at: 619 2388 619
Size: 1769 2424
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SanDI GG'GwC_CC
Cuts at: 278 278
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 506 3710 506
Size: 3204 989
SfcI C'TryA_G
Cuts at: 33 322 1150 1236 1921 2914 3105 33
Size: 289 828 86 685 993 191 1121
Fragments arranged by size:
1121 993 828 685 289 191 86
SfoI GGC'GCC
Cuts at: 988 1625 1873 988
Size: 637 248 3308
Fragments arranged by size:
3308 637 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 243 548 676 1215 243
Size: 305 128 539 3221
Fragments arranged by size:
3221 539 305 128
SmlI C'TyrA_G
Cuts at: 1090 1348 2263 2755 3017 1090
Size: 258 915 492 262 2266
Fragments arranged by size:
2266 915 492 262 258
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StuI AGG'CCT
Cuts at: 146 146
Size: 4193
StyI C'CwwG_G
Cuts at: 107 351 947 1327 1681 107
Size: 244 596 380 354 2619
Fragments arranged by size:
2619 596 380 354 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 669 1776 1909 2368 2577 669
Size: 1107 133 459 209 2285
Fragments arranged by size:
2285 1107 459 209 133
TatI w'GTAC_w
Cuts at: 431 504 3708 431
Size: 73 3204 916
Fragments arranged by size:
3204 916 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4161
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1688 1688
Size: 4193
XmaI C'CCGG_G
Cuts at: 241 546 674 1213 241
Size: 305 128 539 3221
Fragments arranged by size:
3221 539 305 128
XmnI GAAnn'nnTTC
Cuts at: 753 865 753
Size: 112 4081
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI