(Linear) (Six-base) MAPSORT of: KIEE2488.seq Check: 7226 from: 1 to: 1080 KIEE2488.orf With 182 enzymes: SgfI * October 10, 2013 10:36 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 1080 Size: 757 323 AleI CACnn'nnGTG Cuts at: 0 375 1080 Size: 375 705 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 239 1080 Size: 239 841 ApaI G_GGCC'C Cuts at: 0 93 447 1080 Size: 93 354 633 Fragments arranged by size: 633 354 93 AvaI C'yCGr_G Cuts at: 0 55 133 438 566 1080 Size: 55 78 305 128 514 Fragments arranged by size: 514 305 128 78 55 BanI G'GyrC_C Cuts at: 0 369 552 878 1080 Size: 369 183 326 202 Fragments arranged by size: 369 326 202 183 BanII G_rGCy'C Cuts at: 0 93 134 447 521 1080 Size: 93 41 313 74 559 Fragments arranged by size: 559 313 93 74 41 BbeI G_GCGC'C Cuts at: 0 882 1080 Size: 882 198 BbsI GAAGACnn'nnnn_ Cuts at: 0 196 1080 Size: 196 884 Bme1580I G_kGCm'C Cuts at: 0 93 447 555 1080 Size: 93 354 108 525 Fragments arranged by size: 525 354 108 93 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 556 581 880 949 1080 Size: 319 237 25 299 69 131 Fragments arranged by size: 319 299 237 131 69 25 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 967 1080 Size: 967 113 BsaI GGTCTCn'nnnn_ Cuts at: 0 143 1080 Size: 143 937 BsaHI Gr'CG_yC Cuts at: 0 879 1080 Size: 879 201 BsaWI w'CCGG_w Cuts at: 0 513 1080 Size: 513 567 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 91 121 151 1080 Size: 91 30 30 929 Fragments arranged by size: 929 91 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 131 379 442 703 877 880 1080 Size: 131 248 63 261 174 3 200 Fragments arranged by size: 261 248 200 174 131 63 3 BseYI C'CCAG_C Cuts at: 0 30 275 449 601 663 1058 1080 Size: 30 245 174 152 62 395 22 Fragments arranged by size: 395 245 174 152 62 30 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 245 1080 Size: 172 73 835 Fragments arranged by size: 835 172 73 BsmBI CGTCTCn'nnnn_ Cuts at: 0 78 601 1080 Size: 78 523 479 Fragments arranged by size: 523 479 78 Bsp1286I G_dGCh'C Cuts at: 0 93 134 447 521 555 1080 Size: 93 41 313 74 34 525 Fragments arranged by size: 525 313 93 74 41 34 BspEI T'CCGG_A Cuts at: 0 513 1080 Size: 513 567 BsrBI CCG'CTC Cuts at: 0 746 939 1080 Size: 746 193 141 Fragments arranged by size: 746 193 141 BsrFI r'CCGG_y Cuts at: 0 465 1065 1080 Size: 465 600 15 Fragments arranged by size: 600 465 15 Bsu36I CC'TnA_GG Cuts at: 0 40 1080 Size: 40 1040 DraIII CAC_nnn'GTG Cuts at: 0 797 1080 Size: 797 283 EaeI y'GGCC_r Cuts at: 0 836 1080 Size: 836 244 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 556 581 757 880 949 1080 Size: 319 237 25 176 123 69 131 Fragments arranged by size: 319 237 176 131 123 69 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 862 1080 Size: 862 218 EcoO109I rG'GnC_Cy Cuts at: 0 90 170 443 444 660 1080 Size: 90 80 273 1 216 420 Fragments arranged by size: 420 273 216 90 80 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 709 1080 Size: 709 371 FspI TGC'GCA Cuts at: 0 790 1080 Size: 790 290 HaeII r_GCGC'y Cuts at: 0 882 903 1080 Size: 882 21 177 Fragments arranged by size: 882 177 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 91 121 123 153 1080 Size: 91 30 2 30 927 Fragments arranged by size: 927 91 30 30 2 KasI G'GCGC_C Cuts at: 0 878 1080 Size: 878 202 MscI TGG'CCA Cuts at: 0 838 1080 Size: 838 242 MslI CAynn'nnrTG Cuts at: 0 375 1080 Size: 375 705 MspA1I CmG'CkG Cuts at: 0 156 601 804 1005 1080 Size: 156 445 203 201 75 Fragments arranged by size: 445 203 201 156 75 NaeI GCC'GGC Cuts at: 0 1067 1080 Size: 1067 13 NarI GG'CG_CC Cuts at: 0 879 1080 Size: 879 201 NgoMIV G'CCGG_C Cuts at: 0 1065 1080 Size: 1065 15 PasI CC'CwG_GG Cuts at: 0 548 1080 Size: 548 532 PpuMI rG'GwC_Cy Cuts at: 0 170 1080 Size: 170 910 PspOMI G'GGCC_C Cuts at: 0 89 443 1080 Size: 89 354 637 Fragments arranged by size: 637 354 89 PstI C_TGCA'G Cuts at: 0 218 1046 1080 Size: 218 828 34 Fragments arranged by size: 828 218 34 PvuII CAG'CTG Cuts at: 0 601 1005 1080 Size: 601 404 75 Fragments arranged by size: 601 404 75 RsrII CG'GwC_CG Cuts at: 0 511 1080 Size: 511 569 SanDI GG'GwC_CC Cuts at: 0 170 1080 Size: 170 910 ScaI AGT'ACT Cuts at: 0 398 1080 Size: 398 682 SfcI C'TryA_G Cuts at: 0 214 1042 1080 Size: 214 828 38 Fragments arranged by size: 828 214 38 SfoI GGC'GCC Cuts at: 0 880 1080 Size: 880 200 SmaI CCC'GGG Cuts at: 0 135 440 568 1080 Size: 135 305 128 512 Fragments arranged by size: 512 305 135 128 SmlI C'TyrA_G Cuts at: 0 982 1080 Size: 982 98 StuI AGG'CCT Cuts at: 0 38 1080 Size: 38 1042 StyI C'CwwG_G Cuts at: 0 243 839 1080 Size: 243 596 241 Fragments arranged by size: 596 243 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 561 1080 Size: 561 519 TatI w'GTAC_w Cuts at: 0 323 396 1080 Size: 323 73 684 Fragments arranged by size: 684 323 73 XmaI C'CCGG_G Cuts at: 0 133 438 566 1080 Size: 133 305 128 514 Fragments arranged by size: 514 305 133 128 XmnI GAAnn'nnTTC Cuts at: 0 645 757 1080 Size: 645 112 323 Fragments arranged by size: 645 323 112 Enzymes that do cut: AcuI AleI AlfI ApaI AvaI BanI BanII BbeI BbsI Bme1580I BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspEI BsrBI BsrFI Bsu36I DraIII EaeI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV PasI PpuMI PspOMI PstI PvuII RsrII SanDI ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI ZraI