Restriction map for pF1KE1039
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BfrI        BglI        
BlnI        BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CfoI        
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
KasI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccII         676  CGCG                 CG^CG
AseI          646  ATTAAT               AT^TAAT
AsnI          646  ATTAAT               AT^TAAT
Asp700I       895  GAAATAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         984  GGACC                G^G[AT]CC
BanI          830  GGCACC               G^G[CT][GA]CC
BanII         489  GAGCCC               G[GA]GC[CT]^C
BciVI        1078  GGATAC               GTATCC(6/5)
BclI          410  TGATCA               T^GATCA
BcnI           31  CCGGG                CC^[GC]GG
BglII        1344  AGATCT               A^GATCT
BlpI          553  GCTAAGC              GC^T[ACGT]AGC
BmyI          489  GAGCCC               G[AGT]GC[ACT]^C
Bpu1102I      553  GCTAAGC              GC^T[ACGT]AGC
BsmFI        1167  GGGAC                GGGAC(10/14)
Bsp1286I      489  GAGCCC               G[AGT]GC[ACT]^C
BspHI          44  TCATGA               T^CATGA
BspLU11I      848  ACATGT               A^CATGT
BspMI         389  GCAGGT               ACCTGC(4/8)
BstUI         676  CGCG                 CG^CG
Cac8I         487  GCGAGC               GC[ACGT]^[ACGT]GC
CelII         553  GCTAAGC              GC^T[ACGT]AGC
EaeI           11  TGGCCG               [CT]^GGCC[GA]
EcoT22I      1161  ATGCAT               ATGCA^T
FauI          620  GCGGG                CCCGC(4/6)
FbaI          410  TGATCA               T^GATCA
HincII        625  GTTAAC               GT[CT]^[GA]AC
HindII        625  GTTAAC               GT[CT]^[GA]AC
HindIII       249  AAGCTT               A^AGCTT
HpaI          625  GTTAAC               GTT^AAC
HphI          295  TCACC                GGTGA(8/7)
MaeII         516  ACGT                 A^CGT
MvnI          676  CGCG                 CG^CG
NciI           31  CCGGG                CC^[GC]GG
NsiI         1161  ATGCAT               ATGCA^T
NspI          848  ACATGT               [GA]CATG^[CT]
PshBI         646  ATTAAT               AT^TAAT
PstI          277  CTGCAG               CTGCA^G
RcaI           44  TCATGA               T^CATGA
XmnI          895  GAAATAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI          844  GTAC                 GT^AC
              852  GTAC                
AflIII        514  ACACGT               A^C[GA][CT]GT
              848  ACATGT              
AlwNI         887  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1051  CAGCTTCTG           
BbsI           48  GAAGAC               GAAGAC(2/6)
              924  GAAGAC              
BpmI          363  CTCCAG               CTGGAG(16/14)
              536  CTCCAG              
BpuAI          48  GAAGAC               GAAGAC(2/6)
              924  GAAGAC              
BsaJI           8  CCCTGG               C^C[ACGT][ACGT]GG
             1367  CCTGGG              
BsiYI          14  CCGAGTCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              613  CCTGTTTGCGG         
BslI           14  CCGAGTCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              613  CCTGTTTGCGG         
BsmAI         511  GAGAC                GTCTC(1/5)
               18  GTCTC               
Bsp1407I      843  TGTACA               T^GTACA
              851  TGTACA              
BsrDI          99  CATTGC               GCAATG(2/0)
              103  GCAATG              
BsrGI         843  TGTACA               T^GTACA
              851  TGTACA              
BsrI          240  ACTGG                ACTGG(1/-1)
             1325  CCAGT               
Cfr13I         33  GGGCC                G^G[ACGT]CC
              984  GGACC               
DraI          375  TTTAAA               TTT^AAA
              765  TTTAAA              
HapII          31  CCGG                 C^CGG
              208  CCGG                
HgaI          558  GCGTC                GACGC(5/10)
              936  GCGTC               
HpaII          31  CCGG                 C^CGG
              208  CCGG                
MslI           99  CATTGCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1036  CATAAGAGTG          
MspI           31  CCGG                 C^CGG
              208  CCGG                
NlaIV         830  GGCACC               GG[ACGT]^[ACGT]CC
             1239  GGAACC              
PvuII         663  CAGCTG               CAG^CTG
              887  CAGCTG              
RsaI          844  GTAC                 GT^AC
              852  GTAC                
Sau96I         33  GGGCC                G^G[ACGT]CC
              984  GGACC               
SspBI         843  TGTACA               T^GTACA
              851  TGTACA              
TspRI         118  CAGTG                CA[GC]TG[ACGT][ACGT]^
              175  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
BbvI        BfaI        BssKI       BstF5I      BstNI       
BstYI       DdeI        DpnII       EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      PleI        Sau3AI      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TthHB8I     
XhoII       
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