(Linear) (Six-base) MAPSORT of: KIEE2680.seq Check: 5059 from: 1 to: 1281 KIEE2680.orf With 182 enzymes: SgfI * March 1, 2017 16:47 .. AatII G_ACGT'C Cuts at: 0 251 1205 1281 Size: 251 954 76 Fragments arranged by size: 954 251 76 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 534 1281 Size: 172 362 747 Fragments arranged by size: 747 362 172 AfeI AGC'GCT Cuts at: 0 400 892 1281 Size: 400 492 389 Fragments arranged by size: 492 400 389 AgeI A'CCGG_T Cuts at: 0 1211 1281 Size: 1211 70 AleI CACnn'nnGTG Cuts at: 0 1165 1281 Size: 1165 116 AvaI C'yCGr_G Cuts at: 0 513 1124 1281 Size: 513 611 157 Fragments arranged by size: 611 513 157 BamHI G'GATC_C Cuts at: 0 106 729 1013 1281 Size: 106 623 284 268 Fragments arranged by size: 623 284 268 106 BanI G'GyrC_C Cuts at: 0 1141 1281 Size: 1141 140 BanII G_rGCy'C Cuts at: 0 56 164 1281 Size: 56 108 1117 Fragments arranged by size: 1117 108 56 BbsI GAAGACnn'nnnn_ Cuts at: 0 282 1281 Size: 282 999 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1165 1199 1281 Size: 1165 34 82 Fragments arranged by size: 1165 82 34 BglII A'GATC_T Cuts at: 0 635 1281 Size: 635 646 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 871 1281 Size: 622 249 410 Fragments arranged by size: 622 410 249 Bpu10I CC'TnA_GC Cuts at: 0 688 1281 Size: 688 593 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 1281 Size: 50 1231 BsaI GGTCTCn'nnnn_ Cuts at: 0 454 751 1281 Size: 454 297 530 Fragments arranged by size: 530 454 297 BsaBI GATnn'nnATC Cuts at: 0 1051 1281 Size: 1051 230 BsaHI Gr'CG_yC Cuts at: 0 248 1202 1281 Size: 248 954 79 Fragments arranged by size: 954 248 79 BsaWI w'CCGG_w Cuts at: 0 1211 1281 Size: 1211 70 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 66 96 961 991 1281 Size: 66 30 865 30 290 Fragments arranged by size: 865 290 66 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 53 904 982 1281 Size: 53 851 78 299 Fragments arranged by size: 851 299 78 53 BseYI C'CCAG_C Cuts at: 0 418 908 1281 Size: 418 490 373 Fragments arranged by size: 490 418 373 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 649 732 757 907 1096 1281 Size: 649 83 25 150 189 185 Fragments arranged by size: 649 189 185 150 83 25 BsiHKAI G_wGCw'C Cuts at: 0 76 164 1281 Size: 76 88 1117 Fragments arranged by size: 1117 88 76 BsmI GAATG_Cn' Cuts at: 0 221 1281 Size: 221 1060 Bsp1286I G_dGCh'C Cuts at: 0 56 76 164 1281 Size: 56 20 88 1117 Fragments arranged by size: 1117 88 56 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1118 1281 Size: 1118 163 BsrFI r'CCGG_y Cuts at: 0 1211 1281 Size: 1211 70 BssSI C'ACGA_G Cuts at: 0 360 532 655 916 1281 Size: 360 172 123 261 365 Fragments arranged by size: 365 360 261 172 123 BstAPI GCAn_nnn'nTGC Cuts at: 0 576 780 1281 Size: 576 204 501 Fragments arranged by size: 576 501 204 BstXI CCAn_nnnn'nTGG Cuts at: 0 130 1281 Size: 130 1151 BstYI r'GATC_y Cuts at: 0 106 635 729 1013 1281 Size: 106 529 94 284 268 Fragments arranged by size: 529 284 268 106 94 Bsu36I CC'TnA_GG Cuts at: 0 1091 1281 Size: 1091 190 BtgI C'CryG_G Cuts at: 0 734 1281 Size: 734 547 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 924 1281 Size: 924 357 BtsI GCAGTG_nn' Cuts at: 0 670 1281 Size: 670 611 DraIII CAC_nnn'GTG Cuts at: 0 576 1119 1281 Size: 576 543 162 Fragments arranged by size: 576 543 162 EaeI y'GGCC_r Cuts at: 0 299 935 1022 1038 1281 Size: 299 636 87 16 243 Fragments arranged by size: 636 299 243 87 16 EarI CTCTTCn'nnn_ Cuts at: 0 38 44 1281 Size: 38 6 1237 Fragments arranged by size: 1237 38 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 631 1026 1281 Size: 631 395 255 Fragments arranged by size: 631 395 255 EcoICRI GAG'CTC Cuts at: 0 162 1281 Size: 162 1119 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 534 622 871 1281 Size: 172 362 88 249 410 Fragments arranged by size: 410 362 249 172 88 EcoNI CCTnn'n_nnAGG Cuts at: 0 256 691 1281 Size: 256 435 590 Fragments arranged by size: 590 435 256 EcoO109I rG'GnC_Cy Cuts at: 0 509 1281 Size: 509 772 HaeII r_GCGC'y Cuts at: 0 402 532 683 894 975 1281 Size: 402 130 151 211 81 306 Fragments arranged by size: 402 306 211 151 130 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 961 993 1052 1084 1281 Size: 961 32 59 32 197 Fragments arranged by size: 961 197 59 32 32 HincII GTy'rAC Cuts at: 0 327 1065 1281 Size: 327 738 216 Fragments arranged by size: 738 327 216 MscI TGG'CCA Cuts at: 0 301 1024 1281 Size: 301 723 257 Fragments arranged by size: 723 301 257 MslI CAynn'nnrTG Cuts at: 0 739 1165 1281 Size: 739 426 116 Fragments arranged by size: 739 426 116 MspA1I CmG'CkG Cuts at: 0 286 609 1008 1281 Size: 286 323 399 273 Fragments arranged by size: 399 323 286 273 NcoI C'CATG_G Cuts at: 0 734 1281 Size: 734 547 NspI r_CATG'y Cuts at: 0 417 618 1089 1281 Size: 417 201 471 192 Fragments arranged by size: 471 417 201 192 PflMI CCAn_nnn'nTGG Cuts at: 0 501 1281 Size: 501 780 PfoI T'CCnGG_A Cuts at: 0 137 268 1205 1281 Size: 137 131 937 76 Fragments arranged by size: 937 137 131 76 PpuMI rG'GwC_Cy Cuts at: 0 509 1281 Size: 509 772 PshAI GACnn'nnGTC Cuts at: 0 286 1281 Size: 286 995 PstI C_TGCA'G Cuts at: 0 785 1281 Size: 785 496 PvuII CAG'CTG Cuts at: 0 609 1281 Size: 609 672 SacI G_AGCT'C Cuts at: 0 164 1281 Size: 164 1117 SanDI GG'GwC_CC Cuts at: 0 509 1281 Size: 509 772 SapI GCTCTTCn'nnn_ Cuts at: 0 44 1281 Size: 44 1237 ScaI AGT'ACT Cuts at: 0 790 1281 Size: 790 491 SexAI A'CCwGG_T Cuts at: 0 860 1281 Size: 860 421 SfcI C'TryA_G Cuts at: 0 307 781 1281 Size: 307 474 500 Fragments arranged by size: 500 474 307 SmaI CCC'GGG Cuts at: 0 515 1126 1281 Size: 515 611 155 Fragments arranged by size: 611 515 155 SmlI C'TyrA_G Cuts at: 0 65 1281 Size: 65 1216 SrfI GCCC'GGGC Cuts at: 0 1126 1281 Size: 1126 155 StuI AGG'CCT Cuts at: 0 1151 1281 Size: 1151 130 StyI C'CwwG_G Cuts at: 0 734 1281 Size: 734 547 TatI w'GTAC_w Cuts at: 0 410 788 1281 Size: 410 378 493 Fragments arranged by size: 493 410 378 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 66 98 238 270 1281 Size: 66 32 140 32 1011 Fragments arranged by size: 1011 140 66 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 240 270 1281 Size: 240 30 1011 Fragments arranged by size: 1011 240 30 XmaI C'CCGG_G Cuts at: 0 513 1124 1281 Size: 513 611 157 Fragments arranged by size: 611 513 157 ZraI GAC'GTC Cuts at: 0 249 1203 1281 Size: 249 954 78 Fragments arranged by size: 954 249 78 Enzymes that do cut: AatII AcuI AfeI AgeI AleI AvaI BamHI BanI BanII BbsI BcgI BglII BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MscI MslI MspA1I NcoI NspI PflMI PfoI PpuMI PshAI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SmaI SmlI SrfI StuI StyI TatI TstI XcmI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AflIII AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BsaAI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TaqII Tth111I XbaI XhoI XmnI