Restriction map for pF1KB5481
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AgeI        AhdI        
Alw44I      AlwI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BcgI        BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     CelII       ClaI        CpoI        
DpnII       DraI        DraIII      EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       FbaI        FseI        FspI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MroI        MslI        MunI        NarI        
NciI        NcoI        NdeI        NdeII       NheI        
NotI        NruI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII         831  CGCG                 CG^CG
AflIII        891  ACATGT               A^C[GA][CT]GT
AocI          103  CCTCAGG              CC^T[ACGT]AGG
AspHI          89  GAGCAC               G[AT]GC[AT]^C
AvaI           30  CTCGAG               C^[CT]CG[GA]G
BalI         1299  TGGCCA               TGG^CCA
BbsI         1161  GTCTTC               GAAGAC(2/6)
BbvCI         466  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1141  GGATAC               GTATCC(6/5)
BfaI         1201  CTAG                 C^TAG
BmyI           89  GAGCAC               G[AGT]GC[ACT]^C
BpmI         1040  CTGGAG               CTGGAG(16/14)
BpuAI        1161  GTCTTC               GAAGAC(2/6)
BsaWI         545  ACCGGA               [AT]^CCGG[AT]
BseRI         607  GAGGAG               GAGGAG(10/8)
BsiHKAI        89  GAGCAC               G[AT]GC[AT]^C
BsiYI         338  CCGCAACAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          338  CCGCAACAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         788  GAGAC                GTCTC(1/5)
BsoBI          30  CTCGAG               C^[CT]CG[GA]G
Bsp1286I       89  GAGCAC               G[AGT]GC[ACT]^C
BspLU11I      891  ACATGT               A^CATGT
BsrFI          19  GCCGGC               [GA]^CCGG[CT]
BstUI         831  CGCG                 CG^CG
Bsu36I        103  CCTCAGG              CC^T[ACGT]AGG
CfoI           79  GCGC                 GCG^C
Cfr10I         19  GCCGGC               [GA]^CCGG[CT]
DrdI          790  GACAGTCTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1299  TGGCCA               [CT]^GGCC[GA]
Eco81I        103  CCTCAGG              CC^T[ACGT]AGG
EcoRV        1267  GATATC               GAT^ATC
EcoT22I       851  ATGCAT               ATGCA^T
HgaI          249  GCGTC                GACGC(5/10)
HhaI           79  GCGC                 GCG^C
HinP1I         79  GCGC                 G^CGC
MaeI         1201  CTAG                 C^TAG
MluNI        1299  TGGCCA               TGG^CCA
MscI         1299  TGGCCA               TGG^CCA
MvnI          831  CGCG                 CG^CG
NaeI           19  GCCGGC               GCC^GGC
NgoMIV         19  GCCGGC               G^CCGGC
NsiI          851  ATGCAT               ATGCA^T
PaeR7I         30  CTCGAG               C^TCGAG
PshAI         510  GACTCATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI           83  CTGCAG               CTGCA^G
SbfI           82  CCTGCAGG             CCTGCA^GG
SphI          531  GCATGC               GCATG^C
Sse8387I       82  CCTGCAGG             CCTGCA^GG
TaqI           31  TCGA                 T^CGA
TthHB8I        31  TCGA                 T^CGA
XhoI           30  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI         992  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1034  CAGCTGCTG           
AvaII         108  GGACC                G^G[AT]CC
             1190  GGTCC               
BsgI          925  CTGCAC               GTGCAG(16/14)
              839  GTGCAG              
BsmFI        1332  GGGAC                GGGAC(10/14)
             1191  GTCCC               
BsmI          370  GAATGC               GAATGC(1/-1)
              549  GAATGC              
BsrI          260  CCAGT                ACTGG(1/-1)
              983  CCAGT               
BstAPI        817  GCAGAAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              973  GCATTTAATGC         
DraII         107  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1189  GGGTCCC             
DsaI          300  CCGTGG               C^C[GA][CT]GG
              759  CCGTGG              
EcoO109I      107  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1189  GGGTCCC             
EcoT14I       554  CCTTGG               C^C[AT][AT]GG
              941  CCAAGG              
FauI          378  GCGGG                CCCGC(4/6)
              832  GCGGG               
HapII          20  CCGG                 C^CGG
              546  CCGG                
HpaII          20  CCGG                 C^CGG
              546  CCGG                
MspA1I         74  CAGCTG               C[AC]G^C[GT]G
             1034  CAGCTG              
MspI           20  CCGG                 C^CGG
              546  CCGG                
PpuMI         107  AGGACCT              [GA]G^G[AT]CC[CT]
             1189  GGGTCCC             
PvuII          74  CAGCTG               CAG^CTG
             1034  CAGCTG              
SfcI           83  CTGCAG               C^T[GA][CT]AG
              582  CTATAG              
StyI          554  CCTTGG               C^C[AT][AT]GG
              941  CCAAGG              
TspRI        1154  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1253  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        Asp700I     
BbvI        BsaJI       BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeIII      MboII       MnlI        MseI        MvaI        
MwoI        NlaIII      NlaIV       NspI        PleI        
Sau96I      ScrFI       SmlI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     XmnI        
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