Restriction map for pF1KB5481
 (Linear) (Six-base) MAPSORT of: KIBB5481.seq  Check: 3992  from: 1  to: 1359



KIBB5481.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     445     850    1222    1359 
   Size:        204     241     405     372     137

  Fragments arranged by size: 

                 405     372     241     204     137
 
AflIII A'CryG_T

Cuts at:      0     891    1359 
   Size:        891     468
 
AlwNI CAG_nnn'CTG

Cuts at:      0     997    1039    1359 
   Size:        997      42     320

  Fragments arranged by size: 

                 997     320      42
 
ApoI r'AATT_y

Cuts at:      0     433     525    1326    1359 
   Size:        433      92     801      33

  Fragments arranged by size: 

                 801     433      92      33
 
AvaI C'yCGr_G

Cuts at:      0      30    1359 
   Size:         30    1329
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1154    1359 
   Size:       1154     205
 
BbvCI CC'TCA_GC

Cuts at:      0     467    1359 
   Size:        467     892
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1135    1359 
   Size:       1135     224
 
BfrBI ATG'CAT

Cuts at:      0     853    1359 
   Size:        853     506
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1061    1359 
   Size:       1061     298
 
Bpu10I CC'TnA_GC

Cuts at:      0     467    1359 
   Size:        467     892
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133    1070    1359 
   Size:        133     937     289

  Fragments arranged by size: 

                 937     289     133
 
BsaWI w'CCGG_w

Cuts at:      0     545    1359 
   Size:        545     814
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     622    1359 
   Size:        622     737
 
BseYI C'CCAG_C

Cuts at:      0     883    1359 
   Size:        883     476
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     860     910    1359 
   Size:        860      50     449

  Fragments arranged by size: 

                 860     449      50
 
BsiHKAI G_wGCw'C

Cuts at:      0      93    1359 
   Size:         93    1266
 
BsmI GAATG_Cn'

Cuts at:      0     376     555    1359 
   Size:        376     179     804

  Fragments arranged by size: 

                 804     376     179
 
Bsp1286I G_dGCh'C

Cuts at:      0      93    1359 
   Size:         93    1266
 
BsrFI r'CCGG_y

Cuts at:      0      19    1359 
   Size:         19    1340
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     823     979    1359 
   Size:        823     156     380

  Fragments arranged by size: 

                 823     380     156
 
Bsu36I CC'TnA_GG

Cuts at:      0     104    1359 
   Size:        104    1255
 
BtgI C'CryG_G

Cuts at:      0     300     759    1359 
   Size:        300     459     600

  Fragments arranged by size: 

                 600     459     300
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     741     776    1359 
   Size:        741      35     583

  Fragments arranged by size: 

                 741     583      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     796    1359 
   Size:        796     563
 
EaeI y'GGCC_r

Cuts at:      0    1299    1359 
   Size:       1299      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     445     850    1061    1222    1359 
   Size:        204     241     405     211     161     137

  Fragments arranged by size: 

                 405     241     211     204     161     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0     108    1190    1359 
   Size:        108    1082     169

  Fragments arranged by size: 

                1082     169     108
 
EcoRV GAT'ATC

Cuts at:      0    1269    1359 
   Size:       1269      90
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     671    1111    1359 
   Size:        671     440     248

  Fragments arranged by size: 

                 671     440     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     755     787    1034    1066    1359 
   Size:        755      32     247      32     293

  Fragments arranged by size: 

                 755     293     247      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1124    1359 
   Size:       1124     235
 
MscI TGG'CCA

Cuts at:      0    1301    1359 
   Size:       1301      58
 
MspA1I CmG'CkG

Cuts at:      0      76    1036    1359 
   Size:         76     960     323

  Fragments arranged by size: 

                 960     323      76
 
NaeI GCC'GGC

Cuts at:      0      21    1359 
   Size:         21    1338
 
NgoMIV G'CCGG_C

Cuts at:      0      19    1359 
   Size:         19    1340
 
NsiI A_TGCA'T

Cuts at:      0     855    1359 
   Size:        855     504
 
NspI r_CATG'y

Cuts at:      0     535     874     895    1221    1359 
   Size:        535     339      21     326     138

  Fragments arranged by size: 

                 535     339     326     138      21
 
PciI A'CATG_T

Cuts at:      0     891    1359 
   Size:        891     468
 
PfoI T'CCnGG_A

Cuts at:      0    1175    1359 
   Size:       1175     184
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     695     727    1359 
   Size:        695      32     632

  Fragments arranged by size: 

                 695     632      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     108    1190    1359 
   Size:        108    1082     169

  Fragments arranged by size: 

                1082     169     108
 
PshAI GACnn'nnGTC

Cuts at:      0     514    1359 
   Size:        514     845
 
PstI C_TGCA'G

Cuts at:      0      87    1359 
   Size:         87    1272
 
PvuII CAG'CTG

Cuts at:      0      76    1036    1359 
   Size:         76     960     323

  Fragments arranged by size: 

                 960     323      76
 
SanDI GG'GwC_CC

Cuts at:      0    1190    1359 
   Size:       1190     169
 
SbfI CC_TGCA'GG

Cuts at:      0      87    1359 
   Size:         87    1272
 
SfcI C'TryA_G

Cuts at:      0      83     582    1359 
   Size:         83     499     777

  Fragments arranged by size: 

                 777     499      83
 
SmlI C'TyrA_G

Cuts at:      0      30     112    1085    1359 
   Size:         30      82     973     274

  Fragments arranged by size: 

                 973     274      82      30
 
SphI G_CATG'C

Cuts at:      0     535    1359 
   Size:        535     824
 
StyI C'CwwG_G

Cuts at:      0     554     941    1359 
   Size:        554     387     418

  Fragments arranged by size: 

                 554     418     387
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     253    1359 
   Size:        253    1106
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     656     688    1359 
   Size:        656      32     671

  Fragments arranged by size: 

                 671     656      32
 
XhoI C'TCGA_G

Cuts at:      0      30    1359 
   Size:         30    1329
 
XmnI GAAnn'nnTTC

Cuts at:      0     634     707    1095    1359 
   Size:        634      73     388     264

  Fragments arranged by size: 

                 634     388     264      73
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     AvaI     BbsI    BbvCI    BciVI
    BfrBI     BpmI   Bpu10I    BpuEI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BsrFI   BstAPI   Bsu36I     BtgI    CspCI
     DrdI     EaeI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I     MmeI
     MscI   MspA1I     NaeI   NgoMIV     NsiI     NspI     PciI     PfoI
     PpiI    PpuMI    PshAI     PstI    PvuII    SanDI     SbfI     SfcI
     SmlI     SphI     StyI    TaqII     TstI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BcgI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     BtsI
     ClaI     DraI   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NarI     NcoI     NdeI
     NheI     NotI     NruI     PacI     PasI    PflMI     PmeI     PmlI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     XcmI     XmaI     ZraI
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