Restriction map for
pF1KE1035
(Linear) (Six-base) MAPSORT of: KIEE1035.seq Check: 7502 from: 1 to: 1284
KIEE1035.orf
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 327 1284
Size: 327 957
AccI GT'mk_AC
Cuts at: 0 182 1284
Size: 182 1102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 826 1284
Size: 502 324 458
Fragments arranged by size:
502 458 324
AfeI AGC'GCT
Cuts at: 0 403 1284
Size: 403 881
AleI CACnn'nnGTG
Cuts at: 0 1168 1284
Size: 1168 116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 742 1284
Size: 742 542
AlwNI CAG_nnn'CTG
Cuts at: 0 64 331 1284
Size: 64 267 953
Fragments arranged by size:
953 267 64
ApoI r'AATT_y
Cuts at: 0 638 1016 1284
Size: 638 378 268
Fragments arranged by size:
638 378 268
AvaI C'yCGr_G
Cuts at: 0 365 1284
Size: 365 919
BanI G'GyrC_C
Cuts at: 0 1144 1284
Size: 1144 140
BanII G_rGCy'C
Cuts at: 0 29 112 1284
Size: 29 83 1172
Fragments arranged by size:
1172 83 29
BbvCI CC'TCA_GC
Cuts at: 0 80 1284
Size: 80 1204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1038 1072 1284
Size: 1038 34 212
Fragments arranged by size:
1038 212 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 535 1284
Size: 535 749
BglI GCCn_nnn'nGGC
Cuts at: 0 1142 1284
Size: 1142 142
BglII A'GATC_T
Cuts at: 0 743 1284
Size: 743 541
BmrI ACTGGGnnnn_n'
Cuts at: 0 341 1284
Size: 341 943
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 423 998 1284
Size: 423 575 286
Fragments arranged by size:
575 423 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 500 1284
Size: 340 160 784
Fragments arranged by size:
784 340 160
Bpu10I CC'TnA_GC
Cuts at: 0 80 1284
Size: 80 1204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 711 874 1284
Size: 139 572 163 410
Fragments arranged by size:
572 410 163 139
BsaAI yAC'GTr
Cuts at: 0 798 1284
Size: 798 486
BsaBI GATnn'nnATC
Cuts at: 0 1255 1284
Size: 1255 29
BsaWI w'CCGG_w
Cuts at: 0 824 1284
Size: 824 460
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1246 1276 1284
Size: 1246 30 8
Fragments arranged by size:
1246 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 985 1251 1284
Size: 985 266 33
Fragments arranged by size:
985 266 33
BseYI C'CCAG_C
Cuts at: 0 64 911 936 1284
Size: 64 847 25 348
Fragments arranged by size:
847 348 64 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1228 1284
Size: 910 318 56
Fragments arranged by size:
910 318 56
BsiHKAI G_wGCw'C
Cuts at: 0 29 1284
Size: 29 1255
BsmBI CGTCTCn'nnnn_
Cuts at: 0 754 1284
Size: 754 530
Bsp1286I G_dGCh'C
Cuts at: 0 29 112 1284
Size: 29 83 1172
Fragments arranged by size:
1172 83 29
BspHI T'CATG_A
Cuts at: 0 671 1284
Size: 671 613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 327 657 1121 1284
Size: 327 330 464 163
Fragments arranged by size:
464 330 327 163
BsrBI CCG'CTC
Cuts at: 0 1062 1284
Size: 1062 222
BsrDI GCAATG_nn'
Cuts at: 0 899 941 1070 1284
Size: 899 42 129 214
Fragments arranged by size:
899 214 129 42
BsrFI r'CCGG_y
Cuts at: 0 1136 1284
Size: 1136 148
BssSI C'ACGA_G
Cuts at: 0 658 1284
Size: 658 626
BstAPI GCAn_nnn'nTGC
Cuts at: 0 783 1284
Size: 783 501
BstXI CCAn_nnnn'nTGG
Cuts at: 0 133 1155 1284
Size: 133 1022 129
Fragments arranged by size:
1022 133 129
BstYI r'GATC_y
Cuts at: 0 701 732 743 1193 1284
Size: 701 31 11 450 91
Fragments arranged by size:
701 450 91 31 11
Bsu36I CC'TnA_GG
Cuts at: 0 230 1094 1284
Size: 230 864 190
Fragments arranged by size:
864 230 190
BtgI C'CryG_G
Cuts at: 0 954 1284
Size: 954 330
BtsI GCAGTG_nn'
Cuts at: 0 296 307 1284
Size: 296 11 977
Fragments arranged by size:
977 296 11
DraI TTT'AAA
Cuts at: 0 999 1284
Size: 999 285
EaeI y'GGCC_r
Cuts at: 0 302 1041 1124 1284
Size: 302 739 83 160
Fragments arranged by size:
739 302 160 83
EarI CTCTTCn'nnn_
Cuts at: 0 467 1284
Size: 467 817
EcoICRI GAG'CTC
Cuts at: 0 27 1284
Size: 27 1257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 500 502 826 1284
Size: 340 160 2 324 458
Fragments arranged by size:
458 340 324 160 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 259 1284
Size: 259 1025
EcoO109I rG'GnC_Cy
Cuts at: 0 84 1284
Size: 84 1200
EcoRI G'AATT_C
Cuts at: 0 1016 1284
Size: 1016 268
HaeII r_GCGC'y
Cuts at: 0 405 1284
Size: 405 879
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 1246 1278 1284
Size: 187 32 1027 32 6
Fragments arranged by size:
1027 187 32 32 6
HincII GTy'rAC
Cuts at: 0 183 770 1284
Size: 183 587 514
Fragments arranged by size:
587 514 183
MscI TGG'CCA
Cuts at: 0 304 1126 1284
Size: 304 822 158
Fragments arranged by size:
822 304 158
MslI CAynn'nnrTG
Cuts at: 0 1168 1284
Size: 1168 116
MspA1I CmG'CkG
Cuts at: 0 64 318 894 1284
Size: 64 254 576 390
Fragments arranged by size:
576 390 254 64
NspI r_CATG'y
Cuts at: 0 420 621 1092 1284
Size: 420 201 471 192
Fragments arranged by size:
471 420 201 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 331 1284
Size: 331 953
PfoI T'CCnGG_A
Cuts at: 0 632 1284
Size: 632 652
PshAI GACnn'nnGTC
Cuts at: 0 620 1284
Size: 620 664
PvuII CAG'CTG
Cuts at: 0 64 318 1284
Size: 64 254 966
Fragments arranged by size:
966 254 64
SacI G_AGCT'C
Cuts at: 0 29 1284
Size: 29 1255
SalI G'TCGA_C
Cuts at: 0 181 1284
Size: 181 1103
ScaI AGT'ACT
Cuts at: 0 793 1284
Size: 793 491
SexAI A'CCwGG_T
Cuts at: 0 863 1284
Size: 863 421
SmlI C'TyrA_G
Cuts at: 0 154 690 853 1284
Size: 154 536 163 431
Fragments arranged by size:
536 431 163 154
SpeI A'CTAG_T
Cuts at: 0 918 1284
Size: 918 366
SspI AAT'ATT
Cuts at: 0 1230 1284
Size: 1230 54
StyI C'CwwG_G
Cuts at: 0 905 1148 1284
Size: 905 243 136
Fragments arranged by size:
905 243 136
TatI w'GTAC_w
Cuts at: 0 791 1284
Size: 791 493
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 241 273 1284
Size: 241 32 1011
Fragments arranged by size:
1011 241 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 243 273 1284
Size: 243 30 1011
Fragments arranged by size:
1011 243 30
XmnI GAAnn'nnTTC
Cuts at: 0 641 1284
Size: 641 643
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlfI AlwNI ApoI
AvaI BanI BanII BbvCI BcgI BciVI BglI BglII
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII MscI MslI
MspA1I NspI PflMI PfoI PshAI PvuII SacI SalI
ScaI SexAI SmlI SpeI SspI StyI TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BclI BfrBI BlpI Bme1580I BmgBI BmtI
BsaI BsaHI BsiEI BsiWI BsmI BspEI BsrGI BssHII
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SanDI SapI
SbfI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI ZraI