Restriction map for
pF1KB6750
(Circular) (Six-base) MAPSORT of: pF1KB6750.seq Check: 4692 from: 1 to: 3548
pF1KB6750 3548 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 354 354
Size: 3548
AccI GT'mk_AC
Cuts at: 586 586
Size: 3548
Acc65I G'GTAC_C
Cuts at: 564 564
Size: 3548
AclI AA'CG_TT
Cuts at: 3388 3388
Size: 3548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 1373 1805 2552 2820 366
Size: 1007 432 747 268 1094
Fragments arranged by size:
1094 1007 747 432 268
AflIII A'CryG_T
Cuts at: 900 2004 2981 900
Size: 1104 977 1467
Fragments arranged by size:
1467 1104 977
AgeI A'CCGG_T
Cuts at: 1936 1936
Size: 3548
AlwNI CAG_nnn'CTG
Cuts at: 853 2420 853
Size: 1567 1981
ApaLI G'TGCA_C
Cuts at: 2318 2318
Size: 3548
ApoI r'AATT_y
Cuts at: 552 2901 3157 552
Size: 2349 256 943
Fragments arranged by size:
2349 943 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3548
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3548
AvaI C'yCGr_G
Cuts at: 568 568
Size: 3548
BamHI G'GATC_C
Cuts at: 573 573
Size: 3548
BanI G'GyrC_C
Cuts at: 293 564 978 1226 1261 293
Size: 271 414 248 35 2580
Fragments arranged by size:
2580 414 271 248 35
BanII G_rGCy'C
Cuts at: 159 562 1592 159
Size: 403 1030 2115
Fragments arranged by size:
2115 1030 403
BbeI G_GCGC'C
Cuts at: 982 1230 982
Size: 248 3300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3514
BciVI GTATCCnnnnn_n'
Cuts at: 204 1446 2207 3548 204
Size: 1242 761 1341 204
Fragments arranged by size:
1341 1242 761 204
BclI T'GATC_A
Cuts at: 1068 1068
Size: 3548
BglII A'GATC_T
Cuts at: 338 495 1063 3100 338
Size: 157 568 2037 786
Fragments arranged by size:
2037 786 568 157
BlpI GC'TnA_GC
Cuts at: 660 660
Size: 3548
Bme1580I G_kGCm'C
Cuts at: 1173 1266 2322 1173
Size: 93 1056 2399
Fragments arranged by size:
2399 1056 93
BmgBI CAC'GTC
Cuts at: 132 132
Size: 3548
BmrI ACTGGGnnnn_n'
Cuts at: 760 841 1176 3320 760
Size: 81 335 2144 988
Fragments arranged by size:
2144 988 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 448 134
Size: 314 3234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1603 2095 2393 724
Size: 879 492 298 1879
Fragments arranged by size:
1879 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3252 3252
Size: 3548
BsaAI yAC'GTr
Cuts at: 901 901
Size: 3548
BsaBI GATnn'nnATC
Cuts at: 6 1062 3099 6
Size: 1056 2037 455
Fragments arranged by size:
2037 1056 455
BsaHI Gr'CG_yC
Cuts at: 351 365 979 1227 1929 3425 351
Size: 14 614 248 702 1496 474
Fragments arranged by size:
1496 702 614 474 248 14
BsaWI w'CCGG_w
Cuts at: 737 1258 1936 2210 2357 2768 737
Size: 521 678 274 147 411 1517
Fragments arranged by size:
1517 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 107 137 107
Size: 30 3518
BseYI C'CCAG_C
Cuts at: 316 373 973 1731 2308 2804 316
Size: 57 600 758 577 496 1060
Fragments arranged by size:
1060 758 600 577 496 57
BsiEI CG_ry'CG
Cuts at: 104 468 1090 1136 2344 104
Size: 364 622 46 1208 1308
Fragments arranged by size:
1308 1208 622 364 46
BsiHKAI G_wGCw'C
Cuts at: 159 562 1340 1530 2322 159
Size: 403 778 190 792 1385
Fragments arranged by size:
1385 792 778 403 190
Bsp1286I G_dGCh'C
Cuts at: 159 562 1173 1266 1340 1530 1592 2322 159
Size: 403 611 93 74 190 62 730 1385
Fragments arranged by size:
1385 730 611 403 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2768 2768
Size: 3548
BspHI T'CATG_A
Cuts at: 2724 2724
Size: 3548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 598 812 1114 1495 598
Size: 214 302 381 2651
Fragments arranged by size:
2651 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1840 1894 3378 3543 50
Size: 1790 54 1484 165 55
Fragments arranged by size:
1790 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1460 1460
Size: 3548
BsrFI r'CCGG_y
Cuts at: 1546 1727 1936 1546
Size: 181 209 3158
Fragments arranged by size:
3158 209 181
BssSI C'ACGA_G
Cuts at: 1819 2177 1819
Size: 358 3190
BstAPI GCAn_nnn'nTGC
Cuts at: 925 925
Size: 3548
BstBI TT'CG_AA
Cuts at: 1909 1909
Size: 3548
BstXI CCAn_nnnn'nTGG
Cuts at: 402 402
Size: 3548
BstYI r'GATC_y
Cuts at: 1 338 495 573 1040 1063 1398 1644 2656
Size: 337 157 78 467 23 335 246 1012
Cuts at: 2656 2742 2754 3100 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 337 335 246 157
86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 482 482
Size: 3548
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1498 1679 1842 1498
Size: 181 163 3204
Fragments arranged by size:
3204 181 163
BtsI GCAGTG_nn'
Cuts at: 791 791
Size: 3548
DraI TTT'AAA
Cuts at: 547 547
Size: 3548
DrdI GACnn_nn'nnGTC
Cuts at: 1254 2112 1254
Size: 858 2690
EaeI y'GGCC_r
Cuts at: 224 465 1133 1307 1698 1725 3070 224
Size: 241 668 174 391 27 1345 702
Fragments arranged by size:
1345 702 668 391 241 174 27
EagI C'GGCC_G
Cuts at: 465 1133 465
Size: 668 2880
EarI CTCTTCn'nnn_
Cuts at: 1571 1781 1571
Size: 210 3338
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1756 2066 2212 3358 1756
Size: 310 146 1146 1946
Fragments arranged by size:
1946 1146 310 146
EcoICRI GAG'CTC
Cuts at: 157 560 157
Size: 403 3145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 366 448 1373 1805 2552 2820 134
Size: 232 82 925 432 747 268 862
Fragments arranged by size:
925 862 747 432 268 232 82
EcoO109I rG'GnC_Cy
Cuts at: 414 687 414
Size: 273 3275
EcoRI G'AATT_C
Cuts at: 552 552
Size: 3548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1567 1567
Size: 3548
FspI TGC'GCA
Cuts at: 1329 1329
Size: 3548
HaeII r_GCGC'y
Cuts at: 317 982 1230 2252 3231 317
Size: 665 248 1022 979 634
Fragments arranged by size:
1022 979 665 634 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1390 1420 1422 1452 1390
Size: 30 2 30 3486
Fragments arranged by size:
3486 30 30 2
HincII GTy'rAC
Cuts at: 587 587
Size: 3548
KasI G'GCGC_C
Cuts at: 978 1226 978
Size: 248 3300
KpnI G_GTAC'C
Cuts at: 568 568
Size: 3548
MluI A'CGCG_T
Cuts at: 2981 2981
Size: 3548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2219 2403 2219
Size: 184 3364
MscI TGG'CCA
Cuts at: 226 1309 226
Size: 1083 2465
MslI CAynn'nnrTG
Cuts at: 1537 1664 1537
Size: 127 3421
MspA1I CmG'CkG
Cuts at: 526 659 973 1333 2346 2591 526
Size: 133 314 360 1013 245 1483
Fragments arranged by size:
1483 1013 360 314 245 133
NaeI GCC'GGC
Cuts at: 1729 1729
Size: 3548
NarI GG'CG_CC
Cuts at: 979 1227 979
Size: 248 3300
NgoMIV G'CCGG_C
Cuts at: 1727 1727
Size: 3548
NotI GC'GGCC_GC
Cuts at: 465 465
Size: 3548
NspI r_CATG'y
Cuts at: 544 601 1101 2008 544
Size: 57 500 907 2084
Fragments arranged by size:
2084 907 500 57
PciI A'CATG_T
Cuts at: 2004 2004
Size: 3548
PflMI CCAn_nnn'nTGG
Cuts at: 426 426
Size: 3548
PfoI T'CCnGG_A
Cuts at: 181 181
Size: 3548
PmeI GTTT'AAAC
Cuts at: 547 547
Size: 3548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 247 279 2687 2719 247
Size: 32 2408 32 1076
Fragments arranged by size:
2408 1076 32 32
PpuMI rG'GwC_Cy
Cuts at: 414 414
Size: 3548
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 250 282 250
Size: 32 3516
PstI C_TGCA'G
Cuts at: 595 1280 595
Size: 685 2863
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3548
PvuII CAG'CTG
Cuts at: 526 973 1333 526
Size: 447 360 2741
Fragments arranged by size:
2741 447 360
RsrII CG'GwC_CG
Cuts at: 1743 1743
Size: 3548
SacI G_AGCT'C
Cuts at: 159 562 159
Size: 403 3145
SalI G'TCGA_C
Cuts at: 585 585
Size: 3548
SapI GCTCTTCn'nnn_
Cuts at: 1571 1781 1571
Size: 210 3338
SbfI CC_TGCA'GG
Cuts at: 595 595
Size: 3548
ScaI AGT'ACT
Cuts at: 3065 3065
Size: 3548
SfcI C'TryA_G
Cuts at: 33 591 1276 2269 2460 33
Size: 558 685 993 191 1121
Fragments arranged by size:
1121 993 685 558 191
SfoI GGC'GCC
Cuts at: 980 1228 980
Size: 248 3300
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3548
SmaI CCC'GGG
Cuts at: 570 570
Size: 3548
SmlI C'TyrA_G
Cuts at: 703 1618 2110 2372 703
Size: 915 492 262 1879
Fragments arranged by size:
1879 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3548
SphI G_CATG'C
Cuts at: 601 1101 601
Size: 500 3048
StyI C'CwwG_G
Cuts at: 682 1036 682
Size: 354 3194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 195 1131 1264 1723 1932 195
Size: 936 133 459 209 1811
Fragments arranged by size:
1811 936 459 209 133
TatI w'GTAC_w
Cuts at: 3063 3063
Size: 3548
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3516
Tth111I GACn'n_nGTC
Cuts at: 1345 1345
Size: 3548
XbaI T'CTAG_A
Cuts at: 579 579
Size: 3548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1043 1043
Size: 3548
XmaI C'CCGG_G
Cuts at: 568 568
Size: 3548
XmnI GAAnn'nnTTC
Cuts at: 262 262
Size: 3548
ZraI GAC'GTC
Cuts at: 352 352
Size: 3548
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmtI
Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI