(Linear) (Six-base) MAPSORT of: KIBB6750.seq  Check: 2844  from: 1  to: 435



KIBB6750.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AatII G_ACGT'C

Cuts at:      0     246     435 
   Size:        246     189
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     435 
   Size:        258     177
 
BanI G'GyrC_C

Cuts at:      0     185     435 
   Size:        185     250
 
BanII G_rGCy'C

Cuts at:      0      51     435 
   Size:         51     384
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      96     435 
   Size:         96     339
 
BglII A'GATC_T

Cuts at:      0     230     387     435 
   Size:        230     157      48

  Fragments arranged by size: 

                 230     157      48
 
BmgBI CAC'GTC

Cuts at:      0      24     435 
   Size:         24     411
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     340     435 
   Size:         26     314      95

  Fragments arranged by size: 

                 314      95      26
 
BsaHI Gr'CG_yC

Cuts at:      0     243     257     435 
   Size:        243      14     178

  Fragments arranged by size: 

                 243     178      14
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      29     435 
   Size:         29     406
 
BseYI C'CCAG_C

Cuts at:      0     208     265     435 
   Size:        208      57     170

  Fragments arranged by size: 

                 208     170      57
 
BsiEI CG_ry'CG

Cuts at:      0     360     435 
   Size:        360      75
 
BsiHKAI G_wGCw'C

Cuts at:      0      51     435 
   Size:         51     384
 
Bsp1286I G_dGCh'C

Cuts at:      0      51     435 
   Size:         51     384
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     294     435 
   Size:        294     141
 
BstYI r'GATC_y

Cuts at:      0     230     387     435 
   Size:        230     157      48

  Fragments arranged by size: 

                 230     157      48
 
Bsu36I CC'TnA_GG

Cuts at:      0     374     435 
   Size:        374      61
 
EaeI y'GGCC_r

Cuts at:      0     116     357     435 
   Size:        116     241      78

  Fragments arranged by size: 

                 241     116      78
 
EagI C'GGCC_G

Cuts at:      0     357     435 
   Size:        357      78
 
EcoICRI GAG'CTC

Cuts at:      0      49     435 
   Size:         49     386
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     258     340     435 
   Size:         26     232      82      95

  Fragments arranged by size: 

                 232      95      82      26
 
EcoO109I rG'GnC_Cy

Cuts at:      0     306     435 
   Size:        306     129
 
HaeII r_GCGC'y

Cuts at:      0     209     435 
   Size:        209     226
 
MscI TGG'CCA

Cuts at:      0     118     435 
   Size:        118     317
 
MspA1I CmG'CkG

Cuts at:      0     418     435 
   Size:        418      17
 
NotI GC'GGCC_GC

Cuts at:      0     357     435 
   Size:        357      78
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     318     435 
   Size:        318     117
 
PfoI T'CCnGG_A

Cuts at:      0      73     435 
   Size:         73     362
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     139     171     435 
   Size:        139      32     264

  Fragments arranged by size: 

                 264     139      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     306     435 
   Size:        306     129
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     142     174     435 
   Size:        142      32     261

  Fragments arranged by size: 

                 261     142      32
 
PvuII CAG'CTG

Cuts at:      0     418     435 
   Size:        418      17
 
SacI G_AGCT'C

Cuts at:      0      51     435 
   Size:         51     384
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      87     435 
   Size:         87     348
 
XmnI GAAnn'nnTTC

Cuts at:      0     154     435 
   Size:        154     281
 
ZraI GAC'GTC

Cuts at:      0     244     435 
   Size:        244     191
 

 Enzymes that do cut:

    AatII     AcuI     BanI    BanII    BciVI    BglII    BmgBI     BpmI
    BsaHI    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I    BstXI    BstYI
   Bsu36I     EaeI     EagI  EcoICRI  Eco57MI EcoO109I    HaeII     MscI
   MspA1I     NotI    PflMI     PfoI     PpiI    PpuMI     PsrI    PvuII
     SacI    TaqII     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI