Restriction map for pF1KE0419
##################################
# NO CUT ENZYMES (  185 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        AluI        AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BanII       BbeI        
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DpnII       DraI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MamI        MboI        MboII       MfeI        
MflI        MluI        MroI        MseI        MslI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmeI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AfaI          147  GTAC                 GT^AC
Alw44I        311  GTGCAC               G^TGCAC
ApaLI         311  GTGCAC               G^TGCAC
AvaI           50  CCCGAG               C^[CT]CG[GA]G
BpmI          457  CTCCAG               CTGGAG(16/14)
BsaI           45  GGTCTC               GGTCTC(1/5)
BsmFI         477  GTCCC                GGGAC(10/14)
BsoBI          50  CCCGAG               C^[CT]CG[GA]G
BspMI          42  GCAGGT               ACCTGC(4/8)
BsrBI         222  CCGCTC               CCGCTC(-3/-3)
BssSI         420  CTCGTG               CACGAG(-5/-1)
BstEII         63  GGTGACC              G^GT[ACGT]ACC
BstF5I        141  CATCC                GGATG(2/0)
BstPI          63  GGTGACC              G^GT[ACGT]ACC
BstXI         318  CCACCACTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII         260  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII        417  CACCTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          373  GACAAACTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          464  CCACGG               C^C[GA][CT]GG
EcoNI         453  CCTCCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      260  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I        63  GGTGACC              G^GT[ACGT]ACC
FokI          141  CATCC                GGATG(9/13)
PpuMI         260  AGGTCCT              [GA]G^G[AT]CC[CT]
RsaI          147  GTAC                 GT^AC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AflIII        305  ACACGT               A^C[GA][CT]GT
              307  ACGTGT              
AvaII          90  GGACC                G^G[AT]CC
              261  GGTCC               
BalI            8  TGGCCA               TGG^CCA
              327  TGGCCA              
BanI          204  GGTGCC               G^G[CT][GA]CC
              298  GGCACC              
BbrPI         181  CACGTG               CAC^GTG
              306  CACGTG              
BfaI           85  CTAG                 C^TAG
              284  CTAG                
BsaAI         181  CACGTG               [CT]AC^GT[GA]
              306  CACGTG              
BsaJI          51  CCGAGG               C^C[ACGT][ACGT]GG
              464  CCACGG              
BseRI         345  CTCCTC               GAGGAG(10/8)
              454  CTCCTC              
BsrI          378  ACTGG                ACTGG(1/-1)
              144  CCAGT               
BssKI           6  CCTGG                ^CC[ACGT]GG
              459  CCAGG               
BstNI           6  CCTGG                CC^[AT]GG
              459  CCAGG               
Cfr13I         90  GGACC                G^G[ACGT]CC
              261  GGTCC               
EaeI            8  TGGCCA               [CT]^GGCC[GA]
              327  TGGCCA              
EcoRII          6  CCTGG                ^CC[AT]GG
              459  CCAGG               
MaeI           85  CTAG                 C^TAG
              284  CTAG                
MaeIII         64  GTGAC                ^GT[ACGT]AC
              358  GTCAC               
MluNI           8  TGGCCA               TGG^CCA
              327  TGGCCA              
MscI            8  TGGCCA               TGG^CCA
              327  TGGCCA              
MvaI            6  CCTGG                CC^[AT]GG
              459  CCAGG               
MwoI          426  GCTGCTGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              432  GCTGCTCCTGC         
NlaIV         204  GGTGCC               GG[ACGT]^[ACGT]CC
              298  GGCACC              
PmaCI         181  CACGTG               CAC^GTG
              306  CACGTG              
PmlI          181  CACGTG               CAC^GTG
              306  CACGTG              
Sau96I         90  GGACC                G^G[ACGT]CC
              261  GGTCC               
ScrFI           6  CCTGG                CC^[ACGT]GG
              459  CCAGG               
SfaNI          17  GCATC                GCATC(5/9)
              395  GCATC               
Tsp45I         64  GTGAC                ^GT[GC]AC
              358  GTCAC               
TspRI         195  CACTG                CA[GC]TG[ACGT][ACGT]^
              362  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AciI        AspHI       BbvI        BmyI        BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    Cac8I       
DdeI        Fnu4HI      HaeIII      HphI        ItaI        
MaeII       MnlI        NlaIII      TseI        
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