Restriction map for pF1KB3282
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       AccII       AccIII      AcyI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
AscI        AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT14I     FauI        FseI        FspI        HapII       
HgaI        Hin1I       HpaI        HpaII       KasI        
KspI        MluI        MroI        MspI        MvnI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NotI        NruI        NspI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        Tth111I     
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        494  GGTACC               G^GTACC
AccI          538  GTAGAC               GT^[AC][GT]AC
AclI         1007  AACGTT               AA^CGTT
Alw44I        883  GTGCAC               G^TGCAC
ApaI          763  GGGCCC               GGGCC^C
ApaLI         883  GTGCAC               G^TGCAC
AseI          438  ATTAAT               AT^TAAT
AsnI          438  ATTAAT               AT^TAAT
Asp700I      1441  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       494  GGTACC               G^GTACC
AspHI         883  GTGCAC               G[AT]GC[AT]^C
BanI          494  GGTACC               G^G[CT][GA]CC
BcgI         1919  GCATTGATGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          587  TGATCA               T^GATCA
BglII        1734  AGATCT               A^GATCT
BsaI          208  GAGACC               GGTCTC(1/5)
BsgI          325  CTGCAC               GTGCAG(16/14)
BsiHKAI       883  GTGCAC               G[AT]GC[AT]^C
BsmFI        1532  GTCCC                GGGAC(10/14)
Bsp1407I     1268  TGTACA               T^GTACA
BspHI        1022  TCATGA               T^CATGA
BsrDI         199  CATTGC               GCAATG(2/0)
BsrGI        1268  TGTACA               T^GTACA
BstYI        1734  AGATCT               [GA]^GATC[CT]
CfoI         2049  GCGC                 GCG^C
DraI          129  TTTAAA               TTT^AAA
DraIII        328  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          144  CCACGG               C^C[GA][CT]GG
EarI         1558  CTCTTC               CTCTTC(1/4)
FbaI          587  TGATCA               T^GATCA
HaeII        2048  GGCGCT               [GA]GCGC^[CT]
HhaI         2049  GCGC                 GCG^C
HinP1I       2049  GCGC                 G^CGC
HincII        478  GTCAAC               GT[CT]^[GA]AC
HindII        478  GTCAAC               GT[CT]^[GA]AC
HindIII       971  AAGCTT               A^AGCTT
KpnI          494  GGTACC               GGTAC^C
Ksp632I      1558  CTCTTC               CTCTTC(1/4)
MflI         1734  AGATCT               [GA]^GATC[CT]
MslI          544  CACGCCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         23  CAGCTG               C[AC]G^C[GT]G
NheI         2118  GCTAGC               G^CTAGC
PshBI         438  ATTAAT               AT^TAAT
Psp1406I     1007  AACGTT               AA^CGTT
PstI          176  CTGCAG               CTGCA^G
PvuII          23  CAGCTG               CAG^CTG
RcaI         1022  TCATGA               T^CATGA
SfcI          176  CTGCAG               C^T[GA][CT]AG
SspBI        1268  TGTACA               T^GTACA
XcmI         1347  CCATAGCAAGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1734  AGATCT               [GA]^GATC[CT]
XmnI         1441  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AciI           85  GCGG                 CCGC(-3/-1)
              876  GCGG                
AlwI         1422  GGATC                GGATC(4/5)
             1674  GGATC               
AlwNI          20  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1033  CAGTTCCTG           
AvaI          888  CTCGAG               C^[CT]CG[GA]G
             1262  CTCGAG              
AvaII          41  GGACC                G^G[AT]CC
             1531  GGTCC               
BalI          141  TGGCCA               TGG^CCA
             1125  TGGCCA              
BanII         763  GGGCCC               G[GA]GC[CT]^C
             1576  GGGCTC              
BciVI        2064  GGATAC               GTATCC(6/5)
             1745  GTATCC              
BpmI          233  CTGGAG               CTGGAG(16/14)
             1193  CTGGAG              
BsaBI         456  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1417  GATGTGGATC          
BsaJI         144  CCACGG               C^C[ACGT][ACGT]GG
              418  CCTCGG              
BseRI        1616  CTCCTC               GAGGAG(10/8)
              746  GAGGAG              
BsoBI         888  CTCGAG               C^[CT]CG[GA]G
             1262  CTCGAG              
EaeI          141  TGGCCA               [CT]^GGCC[GA]
             1125  TGGCCA              
EcoRI        1565  GAATTC               G^AATTC
             2126  GAATTC              
EcoT22I      1513  ATGCAT               ATGCA^T
             1708  ATGCAT              
HphI         1117  GGTGA                GGTGA(8/7)
             2035  GGTGA               
MaeII        1008  ACGT                 A^CGT
             1743  ACGT                
MamI          456  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1417  GATGTGGATC          
MfeI          758  CAATTG               C^AATTG
             2059  CAATTG              
MluNI         141  TGGCCA               TGG^CCA
             1125  TGGCCA              
MscI          141  TGGCCA               TGG^CCA
             1125  TGGCCA              
MunI          758  CAATTG               C^AATTG
             2059  CAATTG              
NsiI         1513  ATGCAT               ATGCA^T
             1708  ATGCAT              
PaeR7I        888  CTCGAG               C^TCGAG
             1262  CTCGAG              
PflMI         654  CCAATCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1731  CCAAGATCTGG         
Van91I        654  CCAATCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1731  CCAAGATCTGG         
XhoI          888  CTCGAG               C^TCGAG
             1262  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BbsI        
BbvI        BfaI        BmyI        BpuAI       BsiYI       
BslI        BsmAI       BsmI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MvaI        
MwoI        NdeI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com