Restriction map for pF1KB3282
 (Circular) (Six-base) MAPSORT of: pF1KB3282.seq  Check: 2877  from: 1  to: 5333



pF1KB3282  5333 bp

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

AccI GT'mk_AC

Cuts at:    647    2371     647 
   Size:       1724    3609
 
Acc65I G'GTAC_C

Cuts at:    602    2349     602 
   Size:       1747    3586
 
AclI AA'CG_TT

Cuts at:   1116    5173    1116 
   Size:       4057    1276
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    615    1048    1653    3158    3590    3943    4605     615 
   Size:        433     605    1505     432     353     662    1343

  Fragments arranged by size: 

                1505    1343     662     605     433     432     353
 
AflIII A'CryG_T

Cuts at:   2685    4485    4766    2685 
   Size:       1800     281    3252

  Fragments arranged by size: 

                3252    1800     281
 
AlwNI CAG_nnn'CTG

Cuts at:    133    1146    2638    4076     133 
   Size:       1013    1492    1438    1390

  Fragments arranged by size: 

                1492    1438    1390    1013
 
ApaI G_GGCC'C

Cuts at:    875     875 
   Size:       5333
 
ApaLI G'TGCA_C

Cuts at:    991    4171     991 
   Size:       3180    2153
 
ApoI r'AATT_y

Cuts at:    386     937    1287    1673    2072    2234    2273    2337    4686 
   Size:        551     350     386     399     162      39      64    2349

Cuts at:   4686    4942     386 
   Size:        256     777

  Fragments arranged by size: 

                2349     777     551     399     386     350     256     162
                  64      39
 
AseI AT'TA_AT

Cuts at:     20     547      20 
   Size:        527    4806
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5333
 
AvaI C'yCGr_G

Cuts at:    996    1370    2353     996 
   Size:        374     983    3976

  Fragments arranged by size: 

                3976     983     374
 
BamHI G'GATC_C

Cuts at:   2358    2358 
   Size:       5333
 
BanI G'GyrC_C

Cuts at:    602    2349    2763    3011    3046     602 
   Size:       1747     414     248      35    2889

  Fragments arranged by size: 

                2889    1747     414     248      35
 
BanII G_rGCy'C

Cuts at:    875    1688    2347    3377     875 
   Size:        813     659    1030    2831

  Fragments arranged by size: 

                2831    1030     813     659
 
BbeI G_GCGC'C

Cuts at:   2767    3015    2767 
   Size:        248    5085
 
BbsI GAAGACnn'nnnn_

Cuts at:   1322    1768    2291    1322 
   Size:        446     523    4364

  Fragments arranged by size: 

                4364     523     446
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2016    2050    2016 
   Size:         34    5299
 
BciVI GTATCCnnnnn_n'

Cuts at:   1864    2166    3231    4287    5333    1864 
   Size:        302    1065    1056    1046    1864

  Fragments arranged by size: 

                1864    1065    1056    1046     302
 
BclI T'GATC_A

Cuts at:    695    2853     695 
   Size:       2158    3175
 
BfrBI ATG'CAT

Cuts at:   1623    1818    1623 
   Size:        195    5138
 
BglII A'GATC_T

Cuts at:   1842    2848    4885    1842 
   Size:       1006    2037    2290

  Fragments arranged by size: 

                2290    2037    1006
 
BlpI GC'TnA_GC

Cuts at:   2445    2445 
   Size:       5333
 
Bme1580I G_kGCm'C

Cuts at:    875     995    2958    3051    4175     875 
   Size:        120    1963      93    1124    2033

  Fragments arranged by size: 

                2033    1963    1124     120      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2545    2626    2961    5105    2545 
   Size:         81     335    2144    2773

  Fragments arranged by size: 

                2773    2144     335      81
 
BmtI G_CTAG'C

Cuts at:   2230    2230 
   Size:       5333
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3700    3700 
   Size:       5333
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    362    1322     362 
   Size:        960    4373
 
Bpu10I CC'TnA_GC

Cuts at:   1472    1472 
   Size:       5333
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    438    2509    3388    4102    4400     438 
   Size:       2071     879     714     298    1371

  Fragments arranged by size: 

                2071    1371     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    310    5037     310 
   Size:       4727     606
 
BsaAI yAC'GTr

Cuts at:   2686    2686 
   Size:       5333
 
BsaBI GATnn'nnATC

Cuts at:      6     568    1529    2847    4884       6 
   Size:        562     961    1318    2037     455

  Fragments arranged by size: 

                2037    1318     961     562     455
 
BsaHI Gr'CG_yC

Cuts at:   2764    3012    3714    5210    2764 
   Size:        248     702    1496    2887

  Fragments arranged by size: 

                2887    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2522    3043    3721    4132    4279    4553    2522 
   Size:        521     678     411     147     274    3302

  Fragments arranged by size: 

                3302     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1155    1185    1460    1490    1568    1598    1155 
   Size:         30     275      30      78      30    4890

  Fragments arranged by size: 

                4890     275      78      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    869    1715     869 
   Size:        846    4487
 
BseYI C'CCAG_C

Cuts at:   2758    3516    4181    4589    2758 
   Size:        758     665     408    3502

  Fragments arranged by size: 

                3502     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    418     418 
   Size:       5333
 
BsiEI CG_ry'CG

Cuts at:    104    2875    2921    4151     104 
   Size:       2771      46    1230    1286

  Fragments arranged by size: 

                2771    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    995    2347    3125    3315    4175     995 
   Size:       1352     778     190     860    2153

  Fragments arranged by size: 

                2153    1352     860     778     190
 
BsmI GAATG_Cn'

Cuts at:    354     616    1625     354 
   Size:        262    1009    4062

  Fragments arranged by size: 

                4062    1009     262
 
Bsp1286I G_dGCh'C

Cuts at:    875     995    1688    2347    2958    3051    3125    3315    3377 
   Size:        120     693     659     611      93      74     190      62

Cuts at:   3377    4175     875 
   Size:        798    2033

  Fragments arranged by size: 

                2033     798     693     659     611     190     120      93
                  74      62
 
BspHI T'CATG_A

Cuts at:   1130    3765    1130 
   Size:       2635    2698
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2383    2597    2899    3280    2383 
   Size:        214     302     381    4436

  Fragments arranged by size: 

                4436     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3625    3679    5163    5328      50 
   Size:       3575      54    1484     165      55

  Fragments arranged by size: 

                3575    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    306    3245     306 
   Size:       2939    2394
 
BsrFI r'CCGG_y

Cuts at:   3331    3512    3331 
   Size:        181    5152
 
BsrGI T'GTAC_A

Cuts at:   1376    1376 
   Size:       5333
 
BssSI C'ACGA_G

Cuts at:   3604    4312    3604 
   Size:        708    4625
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2710    2710 
   Size:       5333
 
BstBI TT'CG_AA

Cuts at:   3694    3694 
   Size:       5333
 
BstYI r'GATC_y

Cuts at:      1    1842    2358    2825    2848    3183    3429    3735    3747 
   Size:       1841     516     467      23     335     246     306      12

Cuts at:   3747    3833    4885       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1841    1052     516     467     449     335     306     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    252     252 
   Size:       5333
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3283    3464    3627    3283 
   Size:        181     163    4989

  Fragments arranged by size: 

                4989     181     163
 
BtsI GCAGTG_nn'

Cuts at:    905    1176    1202    1277    1364    2576     905 
   Size:        271      26      75      87    1212    3662

  Fragments arranged by size: 

                3662    1212     271      87      75      26
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1632    1667    1632 
   Size:         35    5298
 
DraI TTT'AAA

Cuts at:    239    2332     239 
   Size:       2093    3240
 
DraIII CAC_nnn'GTG

Cuts at:    441     441 
   Size:       5333
 
DrdI GACnn_nn'nnGTC

Cuts at:   3039    4383    3039 
   Size:       1344    3989
 
EaeI y'GGCC_r

Cuts at:    249    1233    2918    3092    3483    3510    4855     249 
   Size:        984    1685     174     391      27    1345     727

  Fragments arranged by size: 

                1685    1345     984     727     391     174      27
 
EagI C'GGCC_G

Cuts at:   2918    2918 
   Size:       5333
 
EarI CTCTTCn'nnn_

Cuts at:   1672    3356    3566    1672 
   Size:       1684     210    3439

  Fragments arranged by size: 

                3439    1684     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3541    4283    4429    5143    3541 
   Size:        742     146     714    3731

  Fragments arranged by size: 

                3731     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2345    2345 
   Size:       5333
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    362     615    1048    1322    1653    3158    3590    3943    4605 
   Size:        253     433     274     331    1505     432     353     662

Cuts at:   4605     362 
   Size:       1090

  Fragments arranged by size: 

                1505    1090     662     433     432     353     331     274
                 253
 
EcoO109I rG'GnC_Cy

Cuts at:   2472    2472 
   Size:       5333
 
EcoRI G'AATT_C

Cuts at:   1673    2234    2337    1673 
   Size:        561     103    4669

  Fragments arranged by size: 

                4669     561     103
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3352    3352 
   Size:       5333
 
FspI TGC'GCA

Cuts at:   3114    3114 
   Size:       5333
 
HaeII r_GCGC'y

Cuts at:   2160    2767    3015    4245    5016    2160 
   Size:        607     248    1230     771    2477

  Fragments arranged by size: 

                2477    1230     771     607     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1623    1655    1918    1950    2308    2340    3175    3205    3207 
   Size:         32     263      32     358      32     835      30       2

Cuts at:   3207    3237    1623 
   Size:         30    3719

  Fragments arranged by size: 

                3719     835     358     263      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:    588    2372     588 
   Size:       1784    3549
 
HindIII A'AGCT_T

Cuts at:   1079    1079 
   Size:       5333
 
KasI G'GCGC_C

Cuts at:   2763    3011    2763 
   Size:        248    5085
 
KpnI G_GTAC'C

Cuts at:    606    2353     606 
   Size:       1747    3586
 
MfeI C'AATT_G

Cuts at:    866    2167     866 
   Size:       1301    4032
 
MluI A'CGCG_T

Cuts at:   4766    4766 
   Size:       5333
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   4092    4276    4092 
   Size:        184    5149
 
MscI TGG'CCA

Cuts at:    251    1235    3094     251 
   Size:        984    1859    2490

  Fragments arranged by size: 

                2490    1859     984
 
MslI CAynn'nnrTG

Cuts at:    656    3322    3449     656 
   Size:       2666     127    2540

  Fragments arranged by size: 

                2666    2540     127
 
MspA1I CmG'CkG

Cuts at:    133    2444    2758    3118    3902    4147     133 
   Size:       2311     314     360     784     245    1319

  Fragments arranged by size: 

                2311    1319     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   3514    3514 
   Size:       5333
 
NarI GG'CG_CC

Cuts at:   2764    3012    2764 
   Size:        248    5085
 
NdeI CA'TA_TG

Cuts at:    116     198    1733     116 
   Size:         82    1535    3716

  Fragments arranged by size: 

                3716    1535      82
 
NgoMIV G'CCGG_C

Cuts at:   3512    3512 
   Size:       5333
 
NheI G'CTAG_C

Cuts at:   2226    2226 
   Size:       5333
 
NsiI A_TGCA'T

Cuts at:   1625    1820    1625 
   Size:        195    5138
 
NspI r_CATG'y

Cuts at:   2386    2886    4489    2386 
   Size:        500    1603    3230

  Fragments arranged by size: 

                3230    1603     500
 
PciI A'CATG_T

Cuts at:   4485    4485 
   Size:       5333
 
PflMI CCAn_nnn'nTGG

Cuts at:    768    1845     768 
   Size:       1077    4256
 
PmeI GTTT'AAAC

Cuts at:   2332    2332 
   Size:       5333
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3779    3811    3779 
   Size:         32    5301
 
PsiI TTA'TAA

Cuts at:    689    1421     689 
   Size:        732    4601
 
PspOMI G'GGCC_C

Cuts at:    871     871 
   Size:       5333
 
PspXI vC'TCGA_Gb

Cuts at:   1370    1370 
   Size:       5333
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1841    1873    1841 
   Size:         32    5301
 
PstI C_TGCA'G

Cuts at:    288    2380    3065     288 
   Size:       2092     685    2556

  Fragments arranged by size: 

                2556    2092     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5333
 
PvuII CAG'CTG

Cuts at:    133    2758    3118     133 
   Size:       2625     360    2348

  Fragments arranged by size: 

                2625    2348     360
 
RsrII CG'GwC_CG

Cuts at:   3528    3528 
   Size:       5333
 
SacI G_AGCT'C

Cuts at:   2347    2347 
   Size:       5333
 
SalI G'TCGA_C

Cuts at:   2370    2370 
   Size:       5333
 
SapI GCTCTTCn'nnn_

Cuts at:   3356    3566    3356 
   Size:        210    5123
 
SbfI CC_TGCA'GG

Cuts at:   2380    2380 
   Size:       5333
 
ScaI AGT'ACT

Cuts at:   4850    4850 
   Size:       5333
 
SfcI C'TryA_G

Cuts at:     33     284    2376    3061    4029    4220      33 
   Size:        251    2092     685     968     191    1146

  Fragments arranged by size: 

                2092    1146     968     685     251     191
 
SfoI GGC'GCC

Cuts at:   2765    3013    2765 
   Size:        248    5085
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5333
 
SmaI CCC'GGG

Cuts at:   2355    2355 
   Size:       5333
 
SmlI C'TyrA_G

Cuts at:    453     996    1370    2488    3403    4117    4379     453 
   Size:        543     374    1118     915     714     262    1407

  Fragments arranged by size: 

                1407    1118     915     714     543     374     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5333
 
SphI G_CATG'C

Cuts at:   2386    2886    2386 
   Size:        500    4833
 
StyI C'CwwG_G

Cuts at:   2467    2821    2467 
   Size:        354    4979
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2916    3049    3508    3717    2916 
   Size:        133     459     209    4532

  Fragments arranged by size: 

                4532     459     209     133
 
TatI w'GTAC_w

Cuts at:   1376    4848    1376 
   Size:       3472    1861
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    485     517     766     798     881     913    1155    1187    1568 
   Size:         32     249      32      83      32     242      32     381

Cuts at:   1568    1600    4850    4882     485 
   Size:         32    3250      32     936

  Fragments arranged by size: 

                3250     936     381     249     242      83      32      32
                  32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3130    3130 
   Size:       5333
 
XbaI T'CTAG_A

Cuts at:   2364    2364 
   Size:       5333
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1462    2828    1462 
   Size:       1366    3967
 
XhoI C'TCGA_G

Cuts at:    996    1370     996 
   Size:        374    4959
 
XmaI C'CCGG_G

Cuts at:   2353    2353 
   Size:       5333
 
XmnI GAAnn'nnTTC

Cuts at:   1553    1553 
   Size:       5333
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NsiI
     NspI     PciI    PflMI     PmeI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     AscI    AvrII     BaeI    BbvCI     BglI    BmgBI    BsiWI
    BsmBI    BspEI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    EcoNI    EcoRV     FseI    FspAI     HpaI     NcoI     NotI     NruI
     PacI     PasI     PfoI     PmlI    PpuMI    PshAI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com