Restriction map for pF1KB3282
 (Linear) (Six-base) MAPSORT of: KIBB3282.seq  Check: 3202  from: 1  to: 2220



KIBB3282.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

AccI GT'mk_AC

Cuts at:      0     539    2220 
   Size:        539    1681
 
Acc65I G'GTAC_C

Cuts at:      0     494    2220 
   Size:        494    1726
 
AclI AA'CG_TT

Cuts at:      0    1008    2220 
   Size:       1008    1212
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     507     940    1545    2220 
   Size:        507     433     605     675

  Fragments arranged by size: 

                 675     605     507     433
 
AlwNI CAG_nnn'CTG

Cuts at:      0      25    1038    2220 
   Size:         25    1013    1182

  Fragments arranged by size: 

                1182    1013      25
 
ApaI G_GGCC'C

Cuts at:      0     767    2220 
   Size:        767    1453
 
ApaLI G'TGCA_C

Cuts at:      0     883    2220 
   Size:        883    1337
 
ApoI r'AATT_y

Cuts at:      0     278     829    1179    1565    1964    2126    2165    2220 
   Size:        278     551     350     386     399     162      39      55

  Fragments arranged by size: 

                 551     399     386     350     278     162      55      39
            
 
AseI AT'TA_AT

Cuts at:      0     439    2220 
   Size:        439    1781
 
AvaI C'yCGr_G

Cuts at:      0     888    1262    2220 
   Size:        888     374     958

  Fragments arranged by size: 

                 958     888     374
 
BanI G'GyrC_C

Cuts at:      0     494    2220 
   Size:        494    1726
 
BanII G_rGCy'C

Cuts at:      0     767    1580    2220 
   Size:        767     813     640

  Fragments arranged by size: 

                 813     767     640
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1214    1660    2183    2220 
   Size:       1214     446     523      37

  Fragments arranged by size: 

                1214     523     446      37
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1908    1942    2220 
   Size:       1908      34     278

  Fragments arranged by size: 

                1908     278      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1756    2058    2220 
   Size:       1756     302     162

  Fragments arranged by size: 

                1756     302     162
 
BclI T'GATC_A

Cuts at:      0     587    2220 
   Size:        587    1633
 
BfrBI ATG'CAT

Cuts at:      0    1515    1710    2220 
   Size:       1515     195     510

  Fragments arranged by size: 

                1515     510     195
 
BglII A'GATC_T

Cuts at:      0    1734    2220 
   Size:       1734     486
 
Bme1580I G_kGCm'C

Cuts at:      0     767     887    2220 
   Size:        767     120    1333

  Fragments arranged by size: 

                1333     767     120
 
BmtI G_CTAG'C

Cuts at:      0    2122    2220 
   Size:       2122      98
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254    1214    2220 
   Size:        254     960    1006

  Fragments arranged by size: 

                1006     960     254
 
Bpu10I CC'TnA_GC

Cuts at:      0    1364    2220 
   Size:       1364     856
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330    2220 
   Size:        330    1890
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     202    2220 
   Size:        202    2018
 
BsaBI GATnn'nnATC

Cuts at:      0     460    1421    2220 
   Size:        460     961     799

  Fragments arranged by size: 

                 961     799     460
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1047    1077    1352    1382    1460    1490    2220 
   Size:       1047      30     275      30      78      30     730

  Fragments arranged by size: 

                1047     730     275      78      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     761    1607    2220 
   Size:        761     846     613

  Fragments arranged by size: 

                 846     761     613
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310    2220 
   Size:        310    1910
 
BsiHKAI G_wGCw'C

Cuts at:      0     887    2220 
   Size:        887    1333
 
BsmI GAATG_Cn'

Cuts at:      0     246     508    1517    2220 
   Size:        246     262    1009     703

  Fragments arranged by size: 

                1009     703     262     246
 
Bsp1286I G_dGCh'C

Cuts at:      0     767     887    1580    2220 
   Size:        767     120     693     640

  Fragments arranged by size: 

                 767     693     640     120
 
BspHI T'CATG_A

Cuts at:      0    1022    2220 
   Size:       1022    1198
 
BsrDI GCAATG_nn'

Cuts at:      0     198    2220 
   Size:        198    2022
 
BsrGI T'GTAC_A

Cuts at:      0    1268    2220 
   Size:       1268     952
 
BstYI r'GATC_y

Cuts at:      0    1734    2220 
   Size:       1734     486
 
BtgI C'CryG_G

Cuts at:      0     144    2220 
   Size:        144    2076
 
BtsI GCAGTG_nn'

Cuts at:      0     797    1068    1094    1169    1256    2220 
   Size:        797     271      26      75      87     964

  Fragments arranged by size: 

                 964     797     271      87      75      26
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1524    1559    2220 
   Size:       1524      35     661

  Fragments arranged by size: 

                1524     661      35
 
DraI TTT'AAA

Cuts at:      0     131    2220 
   Size:        131    2089
 
DraIII CAC_nnn'GTG

Cuts at:      0     333    2220 
   Size:        333    1887
 
EaeI y'GGCC_r

Cuts at:      0     141    1125    2220 
   Size:        141     984    1095

  Fragments arranged by size: 

                1095     984     141
 
EarI CTCTTCn'nnn_

Cuts at:      0    1564    2220 
   Size:       1564     656
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     507     940    1214    1545    2220 
   Size:        254     253     433     274     331     675

  Fragments arranged by size: 

                 675     433     331     274     254     253
 
EcoRI G'AATT_C

Cuts at:      0    1565    2126    2220 
   Size:       1565     561      94

  Fragments arranged by size: 

                1565     561      94
 
HaeII r_GCGC'y

Cuts at:      0    2052    2220 
   Size:       2052     168
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1515    1547    1810    1842    2200    2220 
   Size:       1515      32     263      32     358      20

  Fragments arranged by size: 

                1515     358     263      32      32      20
 
HincII GTy'rAC

Cuts at:      0     480    2220 
   Size:        480    1740
 
HindIII A'AGCT_T

Cuts at:      0     971    2220 
   Size:        971    1249
 
KpnI G_GTAC'C

Cuts at:      0     498    2220 
   Size:        498    1722
 
MfeI C'AATT_G

Cuts at:      0     758    2059    2220 
   Size:        758    1301     161

  Fragments arranged by size: 

                1301     758     161
 
MscI TGG'CCA

Cuts at:      0     143    1127    2220 
   Size:        143     984    1093

  Fragments arranged by size: 

                1093     984     143
 
MslI CAynn'nnrTG

Cuts at:      0     548    2220 
   Size:        548    1672
 
MspA1I CmG'CkG

Cuts at:      0      25    2220 
   Size:         25    2195
 
NdeI CA'TA_TG

Cuts at:      0       8      90    1625    2220 
   Size:          8      82    1535     595

  Fragments arranged by size: 

                1535     595      82       8
 
NheI G'CTAG_C

Cuts at:      0    2118    2220 
   Size:       2118     102
 
NsiI A_TGCA'T

Cuts at:      0    1517    1712    2220 
   Size:       1517     195     508

  Fragments arranged by size: 

                1517     508     195
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     660    1737    2220 
   Size:        660    1077     483

  Fragments arranged by size: 

                1077     660     483
 
PsiI TTA'TAA

Cuts at:      0     581    1313    2220 
   Size:        581     732     907

  Fragments arranged by size: 

                 907     732     581
 
PspOMI G'GGCC_C

Cuts at:      0     763    2220 
   Size:        763    1457
 
PspXI vC'TCGA_Gb

Cuts at:      0    1262    2220 
   Size:       1262     958
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1733    1765    2220 
   Size:       1733      32     455

  Fragments arranged by size: 

                1733     455      32
 
PstI C_TGCA'G

Cuts at:      0     180    2220 
   Size:        180    2040
 
PvuII CAG'CTG

Cuts at:      0      25    2220 
   Size:         25    2195
 
SfcI C'TryA_G

Cuts at:      0     176    2220 
   Size:        176    2044
 
SmlI C'TyrA_G

Cuts at:      0     345     888    1262    2220 
   Size:        345     543     374     958

  Fragments arranged by size: 

                 958     543     374     345
 
TatI w'GTAC_w

Cuts at:      0    1268    2220 
   Size:       1268     952
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     377     409     658     690     773     805    1047    1079 
   Size:        377      32     249      32      83      32     242      32

Cuts at:   1079    1460    1492    2220 
   Size:        381      32     728

  Fragments arranged by size: 

                 728     381     377     249     242      83      32      32
                  32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1354    2220 
   Size:       1354     866
 
XhoI C'TCGA_G

Cuts at:      0     888    1262    2220 
   Size:        888     374     958

  Fragments arranged by size: 

                 958     888     374
 
XmnI GAAnn'nnTTC

Cuts at:      0    1445    2220 
   Size:       1445     775
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AlwNI     ApaI    ApaLI     ApoI
     AseI     AvaI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI    BglII Bme1580I     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI
    BsrDI    BsrGI    BstYI     BtgI     BtsI    CspCI     DraI   DraIII
     EaeI     EarI  Eco57MI    EcoRI    HaeII    Hin4I   HincII  HindIII
     KpnI     MfeI     MscI     MslI   MspA1I     NdeI     NheI     NsiI
    PflMI     PsiI   PspOMI    PspXI     PsrI     PstI    PvuII     SfcI
     SmlI     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BglI     BlpI    BmgBI     BmrI     BplI    BsaAI    BsaHI
    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI     HpaI     KasI
     MluI     MmeI     NaeI     NarI     NcoI   NgoMIV     NotI     NruI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII  Tth111I     XbaI     XmaI     ZraI
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