Restriction map for pF1KB4148
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BamHI       BbeI        BbvCI       BcgI        BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmAI       BsmBI       
BspEI       BspLU11I    BsrBI       BsrDI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       CpoI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MflI        MluI        
MroI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmeI        PpuMI       PshAI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    StuI        SwaI        Van91I      
XbaI        XhoI        XhoII       XmnI        


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# ONE CUT ENZYMES (   64 enzymes) #
###################################

AccI          967  GTCTAC               GT^[AC][GT]AC
AccII          73  CGCG                 CG^CG
AlwNI        1351  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          930  ATTAAT               AT^TAAT
AsnI          930  ATTAAT               AT^TAAT
AspHI         863  GAGCTC               G[AT]GC[AT]^C
AspI           70  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          303  TGGCCA               TGG^CCA
BanI          948  GGCACC               G^G[CT][GA]CC
BanII         863  GAGCTC               G[GA]GC[CT]^C
BbrPI        1077  CACGTG               CAC^GTG
BciVI         143  GGATAC               GTATCC(6/5)
BfaI         1325  CTAG                 C^TAG
BmyI          863  GAGCTC               G[AGT]GC[ACT]^C
BpmI         1340  CTGGAG               CTGGAG(16/14)
BsaAI        1077  CACGTG               [CT]AC^GT[GA]
BseRI        1270  GAGGAG               GAGGAG(10/8)
BsiHKAI       863  GAGCTC               G[AT]GC[AT]^C
Bsp1286I      863  GAGCTC               G[AGT]GC[ACT]^C
BspDI         852  ATCGAT               AT^CGAT
BspHI         867  TCATGA               T^CATGA
BsrFI          15  GCCGGC               [GA]^CCGG[CT]
BstAPI       1350  GCAGACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         149  CCAGG                CC^[AT]GG
BstUI          73  CGCG                 CG^CG
BstXI        1176  CCAGTTTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          208  GCGC                 GCG^C
Cfr10I         15  GCCGGC               [GA]^CCGG[CT]
ClaI          852  ATCGAT               AT^CGAT
DraI          597  TTTAAA               TTT^AAA
DraII        1298  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DrdI           70  GACCGCGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1438  GAAGAG               CTCTTC(1/4)
EcoO109I     1298  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRII        149  CCAGG                ^CC[AT]GG
EcoT14I       300  CCATGG               C^C[AT][AT]GG
HgaI           74  GCGTC                GACGC(5/10)
HhaI          208  GCGC                 GCG^C
HinP1I        208  GCGC                 G^CGC
Ksp632I      1438  GAAGAG               CTCTTC(1/4)
MaeI         1325  CTAG                 C^TAG
MaeII        1078  ACGT                 A^CGT
MfeI         1232  CAATTG               C^AATTG
MluNI         303  TGGCCA               TGG^CCA
MscI          303  TGGCCA               TGG^CCA
MslI         1311  CACAGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1190  CAGCTG               C[AC]G^C[GT]G
MunI         1232  CAATTG               C^AATTG
MvaI          149  CCAGG                CC^[AT]GG
MvnI           73  CGCG                 CG^CG
NaeI           15  GCCGGC               GCC^GGC
NcoI          300  CCATGG               C^CATGG
NgoMIV         15  GCCGGC               G^CCGGC
NspI         1530  ACATGC               [GA]CATG^[CT]
PflFI          70  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1077  CACGTG               CAC^GTG
PmlI         1077  CACGTG               CAC^GTG
PshBI         930  ATTAAT               AT^TAAT
PvuII        1190  CAGCTG               CAG^CTG
RcaI          867  TCATGA               T^CATGA
SacI          863  GAGCTC               GAGCT^C
StyI          300  CCATGG               C^C[AT][AT]GG
Tth111I        70  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1039  CCACATCCTTACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcsI          826  GAATTT               [GA]^AATT[CT]
             1223  AAATTC              
AfaI          897  GTAC                 GT^AC
             1495  GTAC                
ApoI          826  GAATTT               [GA]^AATT[CT]
             1223  AAATTC              
AvaI           20  CCCGGG               C^[CT]CG[GA]G
               61  CCCGGG              
AvaII         700  GGACC                G^G[AT]CC
              722  GGTCC               
BsgI           26  CTGCAC               GTGCAG(16/14)
              590  CTGCAC              
BsmFI        1549  GGGAC                GGGAC(10/14)
               88  GTCCC               
BsmI          481  GAATGC               GAATGC(1/-1)
             1472  GAATGC              
BsoBI          20  CCCGGG               C^[CT]CG[GA]G
               61  CCCGGG              
Bsp1407I      896  TGTACA               T^GTACA
             1494  TGTACA              
BspMI         348  ACCTGC               ACCTGC(4/8)
              690  ACCTGC              
BsrGI         896  TGTACA               T^GTACA
             1494  TGTACA              
Cac8I          15  GCCGGC               GC[ACGT]^[ACGT]GC
              883  GCAGGC              
DraIII        172  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              282  CACAGTGTG           
DsaI          300  CCATGG               C^C[GA][CT]GG
             1545  CCACGG              
EaeI            2  TGGCCG               [CT]^GGCC[GA]
              303  TGGCCA              
FauI           42  CCCGC                CCCGC(4/6)
               48  CCCGC               
HindIII       554  AAGCTT               A^AGCTT
              566  AAGCTT              
PstI         1193  CTGCAG               CTGCA^G
             1428  CTGCAG              
RsaI          897  GTAC                 GT^AC
             1495  GTAC                
SfaNI         880  GATGC                GCATC(5/9)
             1489  GATGC               
SmaI           20  CCCGGG               CCC^GGG
               61  CCCGGG              
SmlI          875  CTCAAG               C^T[CT][GA]AG
             1294  CTCAAG              
SspBI         896  TGTACA               T^GTACA
             1494  TGTACA              
SspI          500  AATATT               AAT^ATT
             1015  AATATT              
XmaCI          20  CCCGGG               C^CCGGG
               61  CCCGGG              
XmaI           20  CCCGGG               C^CCGGG
               61  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AlwI        BbsI        BbvI        
BcnI        BpuAI       BsaJI       BsiYI       BslI        
BsrI        BssKI       BstF5I      Cfr13I      DdeI        
DpnII       Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MseI        MspI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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