Restriction map for pF1KB0054
 (Circular) (Six-base) MAPSORT of: pF1KB0054.seq  Check: 7380  from: 1  to: 6221



pF1KB0054  6221 bp

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AccI GT'mk_AC

Cuts at:    881    2319    2606    3259     881 
   Size:       1438     287     653    3843

  Fragments arranged by size: 

                3843    1438     653     287
 
Acc65I G'GTAC_C

Cuts at:   3237    3237 
   Size:       6221
 
AclI AA'CG_TT

Cuts at:   6061    6061 
   Size:       6221
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    511    1028    1617    2156    2242    2692    3067    4046    4478 
   Size:        517     589     539      86     450     375     979     432

Cuts at:   4478    5225    5493     511 
   Size:        747     268    1239

  Fragments arranged by size: 

                1239     979     747     589     539     517     450     432
                 375     268      86
 
AflII C'TTAA_G

Cuts at:   1450    1450 
   Size:       6221
 
AflIII A'CryG_T

Cuts at:   1333    2808    3573    4677    5654    1333 
   Size:       1475     765    1104     977    1900

  Fragments arranged by size: 

                1900    1475    1104     977     765
 
AgeI A'CCGG_T

Cuts at:   4609    4609 
   Size:       6221
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2528    2560    2528 
   Size:         32    6189
 
AlwNI CAG_nnn'CTG

Cuts at:   3526    5093    3526 
   Size:       1567    4654
 
ApaLI G'TGCA_C

Cuts at:    859    4991     859 
   Size:       4132    2089
 
ApoI r'AATT_y

Cuts at:    357     695    1173    1831    1973    3225    5574    5830     357 
   Size:        338     478     658     142    1252    2349     256     748

  Fragments arranged by size: 

                2349    1252     748     658     478     338     256     142
            
 
AseI AT'TA_AT

Cuts at:     20    1349    1682    2474      20 
   Size:       1329     333     792    3767

  Fragments arranged by size: 

                3767    1329     792     333
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6221
 
AvaI C'yCGr_G

Cuts at:   2936    3241    2936 
   Size:        305    5916
 
BamHI G'GATC_C

Cuts at:   3246    3246 
   Size:       6221
 
BanI G'GyrC_C

Cuts at:   3237    3651    3899    3934    3237 
   Size:        414     248      35    5524

  Fragments arranged by size: 

                5524     414     248      35
 
BanII G_rGCy'C

Cuts at:   1861    3235    4265    1861 
   Size:       1374    1030    3817

  Fragments arranged by size: 

                3817    1374    1030
 
BbeI G_GCGC'C

Cuts at:   3655    3903    3655 
   Size:        248    5973
 
BbsI GAAGACnn'nnnn_

Cuts at:    107    1137    3014     107 
   Size:       1030    1877    3314

  Fragments arranged by size: 

                3314    1877    1030
 
BciVI GTATCCnnnnn_n'

Cuts at:   2062    4119    4880    6221    2062 
   Size:       2057     761    1341    2062

  Fragments arranged by size: 

                2062    2057    1341     761
 
BclI T'GATC_A

Cuts at:   2115    2196    3741    2115 
   Size:         81    1545    4595

  Fragments arranged by size: 

                4595    1545      81
 
BfrBI ATG'CAT

Cuts at:    421    1224     421 
   Size:        803    5418
 
BglII A'GATC_T

Cuts at:    295     903    3736    5773     295 
   Size:        608    2833    2037     743

  Fragments arranged by size: 

                2833    2037     743     608
 
BlpI GC'TnA_GC

Cuts at:   2144    3333    2144 
   Size:       1189    5032
 
Bme1580I G_kGCm'C

Cuts at:    831     863    2261    2867    3846    3939    4995     831 
   Size:         32    1398     606     979      93    1056    2057

  Fragments arranged by size: 

                2057    1398    1056     979     606      93      32
 
BmgBI CAC'GTC

Cuts at:   2811    2811 
   Size:       6221
 
BmrI ACTGGGnnnn_n'

Cuts at:   3433    3514    3849    5993    3433 
   Size:         81     335    2144    3661

  Fragments arranged by size: 

                3661    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4588    4588 
   Size:       6221
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    240    2555     240 
   Size:       2315    3906
 
Bpu10I CC'TnA_GC

Cuts at:   1855    2964    1855 
   Size:       1109    5112
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2995    3397    4276    4768    5066    2995 
   Size:        402     879     492     298    4150

  Fragments arranged by size: 

                4150     879     492     402     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   5925    5925 
   Size:       6221
 
BsaAI yAC'GTr

Cuts at:   3574    3574 
   Size:       6221
 
BsaBI GATnn'nnATC

Cuts at:      6    3735    5772       6 
   Size:       3729    2037     455

  Fragments arranged by size: 

                3729    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    112    3652    3900    4602    6098     112 
   Size:       3540     248     702    1496     235

  Fragments arranged by size: 

                3540    1496     702     248     235
 
BsaWI w'CCGG_w

Cuts at:   3410    3931    4609    4883    5030    5441    3410 
   Size:        521     678     274     147     411    4190

  Fragments arranged by size: 

                4190     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2528    2558    2528 
   Size:         30    6191
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   2375    2554    2375 
   Size:        179    6042
 
BseYI C'CCAG_C

Cuts at:   2064    2951    3024    3646    4404    4981    5477    2064 
   Size:        887      73     622     758     577     496    2808

  Fragments arranged by size: 

                2808     887     758     622     577     496      73
 
BsiEI CG_ry'CG

Cuts at:    104    3763    3809    5017     104 
   Size:       3659      46    1208    1308

  Fragments arranged by size: 

                3659    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    863    3235    4013    4203    4995     863 
   Size:       2372     778     190     792    2089

  Fragments arranged by size: 

                2372    2089     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    433     522     625     433 
   Size:         89     103    6029

  Fragments arranged by size: 

                6029     103      89
 
Bsp1286I G_dGCh'C

Cuts at:    831     863    1861    2261    2867    3235    3846    3939    4013 
   Size:         32     998     400     606     368     611      93      74

Cuts at:   4013    4203    4265    4995     831 
   Size:        190      62     730    2057

  Fragments arranged by size: 

                2057     998     730     611     606     400     368     190
                  93      74      62      32
 
BspEI T'CCGG_A

Cuts at:   5441    5441 
   Size:       6221
 
BspHI T'CATG_A

Cuts at:   5397    5397 
   Size:       6221
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    581    3271    3485    3787    4168     581 
   Size:       2690     214     302     381    2634

  Fragments arranged by size: 

                2690    2634     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3129    4513    4567    6051    6216      50 
   Size:       3079    1384      54    1484     165      55

  Fragments arranged by size: 

                3079    1484    1384     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    421    1488    1944    2063    2499    4133     421 
   Size:       1067     456     119     436    1634    2509

  Fragments arranged by size: 

                2509    1634    1067     456     436     119
 
BsrFI r'CCGG_y

Cuts at:   4219    4400    4609    4219 
   Size:        181     209    5831

  Fragments arranged by size: 

                5831     209     181
 
BsrGI T'GTAC_A

Cuts at:    371     371 
   Size:       6221
 
BssSI C'ACGA_G

Cuts at:   4492    4850    4492 
   Size:        358    5863
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1667    3598    1667 
   Size:       1931    4290
 
BstBI TT'CG_AA

Cuts at:    355    1242    4582     355 
   Size:        887    3340    1994

  Fragments arranged by size: 

                3340    1994     887
 
BstEII G'GTnAC_C

Cuts at:   2585    2585 
   Size:       6221
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1969    2877    1969 
   Size:        908    5313
 
BstYI r'GATC_y

Cuts at:      1     295     903    3246    3713    3736    4071    4317    5329 
   Size:        294     608    2343     467      23     335     246    1012

Cuts at:   5329    5415    5427    5773       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                2343    1012     608     467     449     346     335     294
                 246      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    882    2607     882 
   Size:       1725    4496
 
BtgI C'CryG_G

Cuts at:    107     119    2903     107 
   Size:         12    2784    3425

  Fragments arranged by size: 

                3425    2784      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4171    4352    4515    4171 
   Size:        181     163    5877

  Fragments arranged by size: 

                5877     181     163
 
BtsI GCAGTG_nn'

Cuts at:    204     564     860    1005    1330    3464     204 
   Size:        360     296     145     325    2134    2961

  Fragments arranged by size: 

                2961    2134     360     325     296     145
 
ClaI AT'CG_AT

Cuts at:    446    2240     446 
   Size:       1794    4427
 
DraI TTT'AAA

Cuts at:   3220    3220 
   Size:       6221
 
DraIII CAC_nnn'GTG

Cuts at:    855     855 
   Size:       6221
 
DrdI GACnn_nn'nnGTC

Cuts at:   3927    4785    3927 
   Size:        858    5363
 
EaeI y'GGCC_r

Cuts at:    131    3806    3980    4371    4398    5743     131 
   Size:       3675     174     391      27    1345     609

  Fragments arranged by size: 

                3675    1345     609     391     174      27
 
EagI C'GGCC_G

Cuts at:   3806    3806 
   Size:       6221
 
EarI CTCTTCn'nnn_

Cuts at:    530    1103    4244    4454     530 
   Size:        573    3141     210    2297

  Fragments arranged by size: 

                3141    2297     573     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4429    4739    4885    6031    4429 
   Size:        310     146    1146    4619

  Fragments arranged by size: 

                4619    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   3233    3233 
   Size:       6221
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    240     511    1028    1617    2156    2242    2555    2692    3067 
   Size:        271     517     589     539      86     313     137     375

Cuts at:   3067    4046    4478    5225    5493     240 
   Size:        979     432     747     268     968

  Fragments arranged by size: 

                 979     968     747     589     539     517     432     375
                 313     271     268     137      86
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2357    2357 
   Size:       6221
 
EcoO109I rG'GnC_Cy

Cuts at:   2897    3360    2897 
   Size:        463    5758
 
EcoRI G'AATT_C

Cuts at:   1973    3225    1973 
   Size:       1252    4969
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2326    4240    2326 
   Size:       1914    4307
 
FspI TGC'GCA

Cuts at:   4002    4002 
   Size:       6221
 
HaeII r_GCGC'y

Cuts at:   2015    3655    3903    4925    5904    2015 
   Size:       1640     248    1022     979    2332

  Fragments arranged by size: 

                2332    1640    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    831     863    1255    1287    2233    2265    2334    2366    4063 
   Size:         32     392      32     946      32      69      32    1697

Cuts at:   4063    4093    4095    4125     831 
   Size:         30       2      30    2927

  Fragments arranged by size: 

                2927    1697     946     392      69      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   3260    3260 
   Size:       6221
 
HindIII A'AGCT_T

Cuts at:    347     509     347 
   Size:        162    6059
 
KasI G'GCGC_C

Cuts at:   3651    3899    3651 
   Size:        248    5973
 
KpnI G_GTAC'C

Cuts at:   3241    3241 
   Size:       6221
 
MfeI C'AATT_G

Cuts at:    401     626     401 
   Size:        225    5996
 
MluI A'CGCG_T

Cuts at:   1333    5654    1333 
   Size:       4321    1900
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1790    4892    5076    1790 
   Size:       3102     184    2935

  Fragments arranged by size: 

                3102    2935     184
 
MscI TGG'CCA

Cuts at:   3982    3982 
   Size:       6221
 
MslI CAynn'nnrTG

Cuts at:   1967    2264    2875    3016    4210    4337    1967 
   Size:        297     611     141    1194     127    3851

  Fragments arranged by size: 

                3851    1194     611     297     141     127
 
MspA1I CmG'CkG

Cuts at:    196     484    1951    2134    2826    3163    3332    3646    4006 
   Size:        288    1467     183     692     337     169     314     360

Cuts at:   4006    5019    5264     196 
   Size:       1013     245    1153

  Fragments arranged by size: 

                1467    1153    1013     692     360     337     314     288
                 245     183     169
 
NaeI GCC'GGC

Cuts at:   4402    4402 
   Size:       6221
 
NarI GG'CG_CC

Cuts at:   3652    3900    3652 
   Size:        248    5973
 
NcoI C'CATG_G

Cuts at:    107    2903     107 
   Size:       2796    3425
 
NdeI CA'TA_TG

Cuts at:   1704    1704 
   Size:       6221
 
NgoMIV G'CCGG_C

Cuts at:   4400    4400 
   Size:       6221
 
NsiI A_TGCA'T

Cuts at:    423    1226     423 
   Size:        803    5418
 
NspI r_CATG'y

Cuts at:    213     421     614    1818    3274    3774    4681     213 
   Size:        208     193    1204    1456     500     907    1753

  Fragments arranged by size: 

                1753    1456    1204     907     500     208     193
 
PasI CC'CwG_GG

Cuts at:    585    2074     585 
   Size:       1489    4732
 
PciI A'CATG_T

Cuts at:   4677    4677 
   Size:       6221
 
PflMI CCAn_nnn'nTGG

Cuts at:   1867    1939    1867 
   Size:         72    6149
 
PmeI GTTT'AAAC

Cuts at:   3220    3220 
   Size:       6221
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2528    2560    5360    5392    2528 
   Size:         32    2800      32    3357

  Fragments arranged by size: 

                3357    2800      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2897    2897 
   Size:       6221
 
PsiI TTA'TAA

Cuts at:   1025    1229    1025 
   Size:        204    6017
 
PstI C_TGCA'G

Cuts at:   1193    1211    1499    3268    3953    1193 
   Size:         18     288    1769     685    3461

  Fragments arranged by size: 

                3461    1769     685     288      18
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6221
 
PvuII CAG'CTG

Cuts at:    196    1951    2134    2826    3163    3646    4006     196 
   Size:       1755     183     692     337     483     360    2411

  Fragments arranged by size: 

                2411    1755     692     483     360     337     183
 
RsrII CG'GwC_CG

Cuts at:   4416    4416 
   Size:       6221
 
SacI G_AGCT'C

Cuts at:   3235    3235 
   Size:       6221
 
SalI G'TCGA_C

Cuts at:   3258    3258 
   Size:       6221
 
SanDI GG'GwC_CC

Cuts at:   2897    2897 
   Size:       6221
 
SapI GCTCTTCn'nnn_

Cuts at:    530    4244    4454     530 
   Size:       3714     210    2297

  Fragments arranged by size: 

                3714    2297     210
 
SbfI CC_TGCA'GG

Cuts at:   3268    3268 
   Size:       6221
 
ScaI AGT'ACT

Cuts at:   5738    5738 
   Size:       6221
 
SfcI C'TryA_G

Cuts at:     33     921    1189    1207    1495    3139    3264    3949    4942 
   Size:        888     268      18     288    1644     125     685     993

Cuts at:   4942    5133      33 
   Size:        191    1121

  Fragments arranged by size: 

                1644    1121     993     888     685     288     268     191
                 125      18
 
SfoI GGC'GCC

Cuts at:   3653    3901    3653 
   Size:        248    5973
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6221
 
SmaI CCC'GGG

Cuts at:   3243    3243 
   Size:       6221
 
SmlI C'TyrA_G

Cuts at:   1450    2936    2974    3376    4291    4783    5045    1450 
   Size:       1486      38     402     915     492     262    2626

  Fragments arranged by size: 

                2626    1486     915     492     402     262      38
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6221
 
SphI G_CATG'C

Cuts at:    213    1818    3274    3774     213 
   Size:       1605    1456     500    2660

  Fragments arranged by size: 

                2660    1605    1456     500
 
SspI AAT'ATT

Cuts at:   1410    2733    1410 
   Size:       1323    4898
 
StuI AGG'CCT

Cuts at:    896     896 
   Size:       6221
 
StyI C'CwwG_G

Cuts at:    107     473     605    2903    3355    3709     107 
   Size:        366     132    2298     452     354    2619

  Fragments arranged by size: 

                2619    2298     452     366     354     132
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1066    3804    3937    4396    4605    1066 
   Size:       2738     133     459     209    2682

  Fragments arranged by size: 

                2738    2682     459     209     133
 
TatI w'GTAC_w

Cuts at:    371     504     530     545    1185    2691    5736     371 
   Size:        133      26      15     640    1506    3045     856

  Fragments arranged by size: 

                3045    1506     856     640     133      26      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2070    2102    5738    5770    2070 
   Size:         32    3636      32    2521

  Fragments arranged by size: 

                3636    2521      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   4018    4018 
   Size:       6221
 
XbaI T'CTAG_A

Cuts at:   3036    3252    3036 
   Size:        216    6005
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3716    3716 
   Size:       6221
 
XhoI C'TCGA_G

Cuts at:   2936    2936 
   Size:       6221
 
XmaI C'CCGG_G

Cuts at:   3241    3241 
   Size:       6221
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AloI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI     PsiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AlfI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI     BglI     BmtI     BsgI    BsiWI
     BsmI   BssHII   Bsu36I    CspCI    EcoRV     FseI    FspAI     HpaI
     NheI     NotI     NruI     PacI     PfoI     PmlI    PshAI   PspOMI
    PspXI     PsrI    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SwaI     XmnI     ZraI
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