Restriction map for pF1KA1150
 (Circular) (Six-base) MAPSORT of: pF1KA1150.seq  Check: 8310  from: 1  to: 4892



pF1KA1150  4892 bp

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

AccI GT'mk_AC

Cuts at:   1930    1930 
   Size:       4892
 
Acc65I G'GTAC_C

Cuts at:   1908    1908 
   Size:       4892
 
AclI AA'CG_TT

Cuts at:   4732    4732 
   Size:       4892
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2717    3149    3502    4164    2717 
   Size:        432     353     662    3445

  Fragments arranged by size: 

                3445     662     432     353
 
AflIII A'CryG_T

Cuts at:    881     897    2244    4044    4325     881 
   Size:         16    1347    1800     281    1448

  Fragments arranged by size: 

                1800    1448    1347     281      16
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1087    1119    1087 
   Size:         32    4860
 
AlwNI CAG_nnn'CTG

Cuts at:   2197    3635    2197 
   Size:       1438    3454
 
ApaI G_GGCC'C

Cuts at:    801     943     801 
   Size:        142    4750
 
ApaLI G'TGCA_C

Cuts at:   3730    3730 
   Size:       4892
 
ApoI r'AATT_y

Cuts at:    819    1896    4245    4501     819 
   Size:       1077    2349     256    1210

  Fragments arranged by size: 

                2349    1210    1077     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4892
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4892
 
AvaI C'yCGr_G

Cuts at:   1912    1912 
   Size:       4892
 
BamHI G'GATC_C

Cuts at:   1917    1917 
   Size:       4892
 
BanI G'GyrC_C

Cuts at:    282    1713    1908    2322    2570    2605     282 
   Size:       1431     195     414     248      35    2569

  Fragments arranged by size: 

                2569    1431     414     248     195      35
 
BanII G_rGCy'C

Cuts at:    801     943    1906    2936     801 
   Size:        142     963    1030    2757

  Fragments arranged by size: 

                2757    1030     963     142
 
BbeI G_GCGC'C

Cuts at:   2326    2574    2326 
   Size:        248    4644
 
BbsI GAAGACnn'nnnn_

Cuts at:    709     709 
   Size:       4892
 
BbvCI CC'TCA_GC

Cuts at:    613     613 
   Size:       4892
 
BciVI GTATCCnnnnn_n'

Cuts at:   2790    3846    4892    2790 
   Size:       1056    1046    2790

  Fragments arranged by size: 

                2790    1056    1046
 
BclI T'GATC_A

Cuts at:   2412    2412 
   Size:       4892
 
BfrBI ATG'CAT

Cuts at:   1498    1498 
   Size:       4892
 
BglI GCCn_nnn'nGGC

Cuts at:   1168    1168 
   Size:       4892
 
BglII A'GATC_T

Cuts at:   2407    4444    2407 
   Size:       2037    2855
 
BlpI GC'TnA_GC

Cuts at:   2004    2004 
   Size:       4892
 
Bme1580I G_kGCm'C

Cuts at:    801     943    1371    2517    2610    3734     801 
   Size:        142     428    1146      93    1124    1959

  Fragments arranged by size: 

                1959    1146    1124     428     142      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1365    1713    1799    2104    2185    2520    4664    1365 
   Size:        348      86     305      81     335    2144    1593

  Fragments arranged by size: 

                2144    1593     348     335     305      86      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3259    3259 
   Size:       4892
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    692    1222    1562    1645     692 
   Size:        530     340      83    3939

  Fragments arranged by size: 

                3939     530     340      83
 
Bpu10I CC'TnA_GC

Cuts at:    613     947     613 
   Size:        334    4558
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    298    2068    2947    3661    3959     298 
   Size:       1770     879     714     298    1231

  Fragments arranged by size: 

                1770    1231     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1600    4596    1600 
   Size:       2996    1896
 
BsaAI yAC'GTr

Cuts at:   2245    2245 
   Size:       4892
 
BsaBI GATnn'nnATC

Cuts at:      6    2406    4443       6 
   Size:       2400    2037     455

  Fragments arranged by size: 

                2400    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2323    2571    3273    4769    2323 
   Size:        248     702    1496    2446

  Fragments arranged by size: 

                2446    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2081    2602    3280    3691    3838    4112    2081 
   Size:        521     678     411     147     274    2861

  Fragments arranged by size: 

                2861     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1560    1590    1560 
   Size:         30    4862
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    601     601 
   Size:       4892
 
BseYI C'CCAG_C

Cuts at:    165     915     920    1123    2317    3075    3740    4148     165 
   Size:        750       5     203    1194     758     665     408     909

  Fragments arranged by size: 

                1194     909     758     750     665     408     203       5
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1274    1274 
   Size:       4892
 
BsiEI CG_ry'CG

Cuts at:    104    2434    2480    3710     104 
   Size:       2330      46    1230    1286

  Fragments arranged by size: 

                2330    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1906    2684    2874    3734    1906 
   Size:        778     190     860    3064

  Fragments arranged by size: 

                3064     860     778     190
 
BsmI GAATG_Cn'

Cuts at:    726     726 
   Size:       4892
 
Bsp1286I G_dGCh'C

Cuts at:    801     943    1371    1906    2517    2610    2684    2874    2936 
   Size:        142     428     535     611      93      74     190      62

Cuts at:   2936    3734     801 
   Size:        798    1959

  Fragments arranged by size: 

                1959     798     611     535     428     190     142      93
                  74      62
 
BspHI T'CATG_A

Cuts at:   1613    3324    1613 
   Size:       1711    3181
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1942    2156    2458    2839    1942 
   Size:        214     302     381    3995

  Fragments arranged by size: 

                3995     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     591    1688    3184    3238    4722    4887      50 
   Size:        541    1097    1496      54    1484     165      55

  Fragments arranged by size: 

                1496    1484    1097     541     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    247    1763    2804     247 
   Size:       1516    1041    2335

  Fragments arranged by size: 

                2335    1516    1041
 
BsrFI r'CCGG_y

Cuts at:   1484    2890    3071    1484 
   Size:       1406     181    3305

  Fragments arranged by size: 

                3305    1406     181
 
BsrGI T'GTAC_A

Cuts at:    714     714 
   Size:       4892
 
BssSI C'ACGA_G

Cuts at:   3163    3871    3163 
   Size:        708    4184
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2269    2269 
   Size:       4892
 
BstBI TT'CG_AA

Cuts at:   3253    3253 
   Size:       4892
 
BstYI r'GATC_y

Cuts at:      1    1205    1917    2384    2407    2742    2988    3294    3306 
   Size:       1204     712     467      23     335     246     306      12

Cuts at:   3306    3392    4444       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1204    1052     712     467     449     335     306     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     224     107 
   Size:        117    4775
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2842    3023    3186    2842 
   Size:        181     163    4548

  Fragments arranged by size: 

                4548     181     163
 
BtsI GCAGTG_nn'

Cuts at:    321    1441    2135     321 
   Size:       1120     694    3078

  Fragments arranged by size: 

                3078    1120     694
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    866     901     866 
   Size:         35    4857
 
DraI TTT'AAA

Cuts at:   1891    1891 
   Size:       4892
 
DrdI GACnn_nn'nnGTC

Cuts at:   2598    3942    2598 
   Size:       1344    3548
 
EaeI y'GGCC_r

Cuts at:   2477    2651    3042    3069    4414    2477 
   Size:        174     391      27    1345    2955

  Fragments arranged by size: 

                2955    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2477    2477 
   Size:       4892
 
EarI CTCTTCn'nnn_

Cuts at:    137    1178    2915    3125     137 
   Size:       1041    1737     210    1904

  Fragments arranged by size: 

                1904    1737    1041     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3100    3842    3988    4702    3100 
   Size:        742     146     714    3290

  Fragments arranged by size: 

                3290     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1904    1904 
   Size:       4892
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    692    1222    1562    1645    2717    3149    3502    4164     692 
   Size:        530     340      83    1072     432     353     662    1420

  Fragments arranged by size: 

                1420    1072     662     530     432     353     340      83
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1516    1516 
   Size:       4892
 
EcoO109I rG'GnC_Cy

Cuts at:    768     797     939    1801    2031     768 
   Size:         29     142     862     230    3629

  Fragments arranged by size: 

                3629     862     230     142      29
 
EcoRI G'AATT_C

Cuts at:   1896    1896 
   Size:       4892
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    148     174     358    2911     148 
   Size:         26     184    2553    2129

  Fragments arranged by size: 

                2553    2129     184      26
 
FspI TGC'GCA

Cuts at:   2673    2673 
   Size:       4892
 
HaeII r_GCGC'y

Cuts at:   1130    2326    2574    3804    4575    1130 
   Size:       1196     248    1230     771    1447

  Fragments arranged by size: 

                1447    1230    1196     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    170     202     657     689    2734    2764    2766    2796     170 
   Size:         32     455      32    2045      30       2      30    2266

  Fragments arranged by size: 

                2266    2045     455      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1761    1931    1761 
   Size:        170    4722
 
HindIII A'AGCT_T

Cuts at:    778     778 
   Size:       4892
 
KasI G'GCGC_C

Cuts at:   2322    2570    2322 
   Size:        248    4644
 
KpnI G_GTAC'C

Cuts at:   1912    1912 
   Size:       4892
 
MluI A'CGCG_T

Cuts at:   4325    4325 
   Size:       4892
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1472    3651    3835    1472 
   Size:       2179     184    2529

  Fragments arranged by size: 

                2529    2179     184
 
MscI TGG'CCA

Cuts at:   2653    2653 
   Size:       4892
 
MslI CAynn'nnrTG

Cuts at:    886    1057    1276    2881    3008     886 
   Size:        171     219    1605     127    2770

  Fragments arranged by size: 

                2770    1605     219     171     127
 
MspA1I CmG'CkG

Cuts at:    612     936    1185    1582    2003    2317    2677    3461    3706 
   Size:        324     249     397     421     314     360     784     245

Cuts at:   3706     612 
   Size:       1798

  Fragments arranged by size: 

                1798     784     421     397     360     324     314     249
                 245
 
NaeI GCC'GGC

Cuts at:   3073    3073 
   Size:       4892
 
NarI GG'CG_CC

Cuts at:   2323    2571    2323 
   Size:        248    4644
 
NcoI C'CATG_G

Cuts at:    107     224     107 
   Size:        117    4775
 
NgoMIV G'CCGG_C

Cuts at:   3071    3071 
   Size:       4892
 
NsiI A_TGCA'T

Cuts at:   1500    1500 
   Size:       4892
 
NspI r_CATG'y

Cuts at:    885    1945    2445    4048     885 
   Size:       1060     500    1603    1729

  Fragments arranged by size: 

                1729    1603    1060     500
 
PciI A'CATG_T

Cuts at:    881    4044     881 
   Size:       3163    1729
 
PfoI T'CCnGG_A

Cuts at:   1199    1199 
   Size:       4892
 
PmeI GTTT'AAAC

Cuts at:   1891    1891 
   Size:       4892
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3338    3370    3338 
   Size:         32    4860
 
PspOMI G'GGCC_C

Cuts at:    797     939     797 
   Size:        142    4750
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1526    1558    1526 
   Size:         32    4860
 
PstI C_TGCA'G

Cuts at:   1161    1939    2624    1161 
   Size:        778     685    3429

  Fragments arranged by size: 

                3429     778     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4892
 
PvuII CAG'CTG

Cuts at:    612    1185    1582    2317    2677     612 
   Size:        573     397     735     360    2827

  Fragments arranged by size: 

                2827     735     573     397     360
 
RsrII CG'GwC_CG

Cuts at:   3087    3087 
   Size:       4892
 
SacI G_AGCT'C

Cuts at:   1906    1906 
   Size:       4892
 
SalI G'TCGA_C

Cuts at:   1929    1929 
   Size:       4892
 
SapI GCTCTTCn'nnn_

Cuts at:    137    1178    2915    3125     137 
   Size:       1041    1737     210    1904

  Fragments arranged by size: 

                1904    1737    1041     210
 
SbfI CC_TGCA'GG

Cuts at:   1939    1939 
   Size:       4892
 
ScaI AGT'ACT

Cuts at:   4409    4409 
   Size:       4892
 
SfcI C'TryA_G

Cuts at:     33     679     925    1157    1513    1935    2620    3588    3779 
   Size:        646     246     232     356     422     685     968     191

Cuts at:   3779      33 
   Size:       1146

  Fragments arranged by size: 

                1146     968     685     646     422     356     246     232
                 191
 
SfoI GGC'GCC

Cuts at:   2324    2572    2324 
   Size:        248    4644
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4892
 
SmaI CCC'GGG

Cuts at:   1914    1914 
   Size:       4892
 
SmlI C'TyrA_G

Cuts at:    277    2047    2962    3676    3938     277 
   Size:       1770     915     714     262    1231

  Fragments arranged by size: 

                1770    1231     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4892
 
SphI G_CATG'C

Cuts at:   1945    2445    1945 
   Size:        500    4392
 
StuI AGG'CCT

Cuts at:    360     360 
   Size:       4892
 
StyI C'CwwG_G

Cuts at:    107     224     801    1320    1743    2026    2380     107 
   Size:        117     577     519     423     283     354    2619

  Fragments arranged by size: 

                2619     577     519     423     354     283     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    990    1832    2475    2608    3067    3276     990 
   Size:        842     643     133     459     209    2606

  Fragments arranged by size: 

                2606     842     643     459     209     133
 
TatI w'GTAC_w

Cuts at:    714    4407     714 
   Size:       3693    1199
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1560    1592    4409    4441    1560 
   Size:         32    2817      32    2011

  Fragments arranged by size: 

                2817    2011      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    671    2689     671 
   Size:       2018    2874
 
XbaI T'CTAG_A

Cuts at:   1923    1923 
   Size:       4892
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    528    2387     528 
   Size:       1859    3033
 
XmaI C'CCGG_G

Cuts at:   1912    1912 
   Size:       4892
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI
     PciI     PfoI     PmeI     PpiI   PspOMI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AscI    AvrII     BaeI     BcgI    BmgBI     BmtI    BsiWI    BsmBI
    BspEI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI   DraIII
    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI     NheI     NotI
     NruI     PacI     PasI    PflMI     PmlI    PpuMI    PshAI     PsiI
    PspXI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     XmnI     ZraI
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