Restriction map for pF1KB9876
 (Circular) (Six-base) MAPSORT of: pF1KB9876.seq  Check: 1096  from: 1  to: 4739



pF1KB9876  4739 bp

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1120    1120 
   Size:       4739
 
AccI GT'mk_AC

Cuts at:   1777    1777 
   Size:       4739
 
Acc65I G'GTAC_C

Cuts at:    675    1755     675 
   Size:       1080    3659
 
AclI AA'CG_TT

Cuts at:   4579    4579 
   Size:       4739
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    260     602     683    1444    1580    2564    2996    3743    4011 
   Size:        342      81     761     136     984     432     747     268

Cuts at:   4011     260 
   Size:        988

  Fragments arranged by size: 

                 988     984     761     747     432     342     268     136
                  81
 
AflIII A'CryG_T

Cuts at:    362    2091    3195    4172     362 
   Size:       1729    1104     977     929

  Fragments arranged by size: 

                1729    1104     977     929
 
AgeI A'CCGG_T

Cuts at:   3127    3127 
   Size:       4739
 
AhdI GACnn_n'nnGTC

Cuts at:    443     545    1653     443 
   Size:        102    1108    3529

  Fragments arranged by size: 

                3529    1108     102
 
AlwNI CAG_nnn'CTG

Cuts at:   2044    3611    2044 
   Size:       1567    3172
 
ApaI G_GGCC'C

Cuts at:   1193    1193 
   Size:       4739
 
ApaLI G'TGCA_C

Cuts at:   3509    3509 
   Size:       4739
 
ApoI r'AATT_y

Cuts at:    153     267     318    1069    1371    1743    4092    4348     153 
   Size:        114      51     751     302     372    2349     256     544

  Fragments arranged by size: 

                2349     751     544     372     302     256     114      51
            
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4739
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4739
 
AvaI C'yCGr_G

Cuts at:   1759    1759 
   Size:       4739
 
BamHI G'GATC_C

Cuts at:   1764    1764 
   Size:       4739
 
BanI G'GyrC_C

Cuts at:    675    1755    2169    2417    2452     675 
   Size:       1080     414     248      35    2962

  Fragments arranged by size: 

                2962    1080     414     248      35
 
BanII G_rGCy'C

Cuts at:    493    1193    1753    2783     493 
   Size:        700     560    1030    2449

  Fragments arranged by size: 

                2449    1030     700     560
 
BbeI G_GCGC'C

Cuts at:   2173    2421    2173 
   Size:        248    4491
 
BbsI GAAGACnn'nnnn_

Cuts at:    455     701     991    1106    1649    1687     455 
   Size:        246     290     115     543      38    3507

  Fragments arranged by size: 

                3507     543     290     246     115      38
 
BbvCI CC'TCA_GC

Cuts at:   1009    1009 
   Size:       4739
 
BciVI GTATCCnnnnn_n'

Cuts at:   2637    3398    4739    2637 
   Size:        761    1341    2637

  Fragments arranged by size: 

                2637    1341     761
 
BclI T'GATC_A

Cuts at:   2259    2259 
   Size:       4739
 
BglII A'GATC_T

Cuts at:    198    2254    4291     198 
   Size:       2056    2037     646

  Fragments arranged by size: 

                2056    2037     646
 
BlpI GC'TnA_GC

Cuts at:   1851    1851 
   Size:       4739
 
Bme1580I G_kGCm'C

Cuts at:   1193    2364    2457    3513    1193 
   Size:       1171      93    1056    2419

  Fragments arranged by size: 

                2419    1171    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    506    1951    2032    2367    4511     506 
   Size:       1445      81     335    2144     734

  Fragments arranged by size: 

                2144    1445     734     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3106    3106 
   Size:       4739
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    590     590 
   Size:       4739
 
Bpu10I CC'TnA_GC

Cuts at:   1009    1009 
   Size:       4739
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1915    2794    3286    3584    1915 
   Size:        879     492     298    3070

  Fragments arranged by size: 

                3070     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    570    4443     570 
   Size:       3873     866
 
BsaAI yAC'GTr

Cuts at:    363    2092     363 
   Size:       1729    3010
 
BsaBI GATnn'nnATC

Cuts at:      6     191     197    2253    4290       6 
   Size:        185       6    2056    2037     455

  Fragments arranged by size: 

                2056    2037     455     185       6
 
BsaHI Gr'CG_yC

Cuts at:   2170    2418    3120    4616    2170 
   Size:        248     702    1496    2293

  Fragments arranged by size: 

                2293    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1928    2449    3127    3401    3548    3959    1928 
   Size:        521     678     274     147     411    2708

  Fragments arranged by size: 

                2708     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    548     578     548 
   Size:         30    4709
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    193     587     193 
   Size:        394    4345
 
BseYI C'CCAG_C

Cuts at:   1094    2164    2922    3499    3995    1094 
   Size:       1070     758     577     496    1838

  Fragments arranged by size: 

                1838    1070     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     436    2281    2327    3535     104 
   Size:        332    1845      46    1208    1308

  Fragments arranged by size: 

                1845    1308    1208     332      46
 
BsiHKAI G_wGCw'C

Cuts at:    493    1514    1753    2531    2721    3513     493 
   Size:       1021     239     778     190     792    1719

  Fragments arranged by size: 

                1719    1021     792     778     239     190
 
Bsp1286I G_dGCh'C

Cuts at:    493    1193    1514    1753    2364    2457    2531    2721    2783 
   Size:        700     321     239     611      93      74     190      62

Cuts at:   2783    3513     493 
   Size:        730    1719

  Fragments arranged by size: 

                1719     730     700     611     321     239     190      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   3959    3959 
   Size:       4739
 
BspHI T'CATG_A

Cuts at:   3915    3915 
   Size:       4739
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1120    1789    2003    2305    2686    1120 
   Size:        669     214     302     381    3173

  Fragments arranged by size: 

                3173     669     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3031    3085    4569    4734      50 
   Size:       2981      54    1484     165      55

  Fragments arranged by size: 

                2981    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    866    2651     866 
   Size:       1785    2954
 
BsrFI r'CCGG_y

Cuts at:    411    1127    1475    2737    2918    3127     411 
   Size:        716     348    1262     181     209    2023

  Fragments arranged by size: 

                2023    1262     716     348     209     181
 
BsrGI T'GTAC_A

Cuts at:    225    1173     225 
   Size:        948    3791
 
BssSI C'ACGA_G

Cuts at:   1568    3010    3368    1568 
   Size:       1442     358    2939

  Fragments arranged by size: 

                2939    1442     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2116    2116 
   Size:       4739
 
BstBI TT'CG_AA

Cuts at:   3100    3100 
   Size:       4739
 
BstYI r'GATC_y

Cuts at:      1     192     198     743     828    1764    2231    2254    2589 
   Size:        191       6     545      85     936     467      23     335

Cuts at:   2589    2835    3847    3933    3945    4291       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     936     545     467     449     346     335     246
                 191      86      85      23      12       6
 
BtgI C'CryG_G

Cuts at:    107    1184     107 
   Size:       1077    3662
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1164    2689    2870    3033    1164 
   Size:       1525     181     163    2870

  Fragments arranged by size: 

                2870    1525     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1119    1982    1119 
   Size:        863    3876
 
DraI TTT'AAA

Cuts at:   1002    1539    1738    1002 
   Size:        537     199    4003

  Fragments arranged by size: 

                4003     537     199
 
DrdI GACnn_nn'nnGTC

Cuts at:   2445    3303    2445 
   Size:        858    3881
 
EaeI y'GGCC_r

Cuts at:   2324    2498    2889    2916    4261    2324 
   Size:        174     391      27    1345    2802

  Fragments arranged by size: 

                2802    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2324    2324 
   Size:       4739
 
EarI CTCTTCn'nnn_

Cuts at:   2762    2972    2762 
   Size:        210    4529
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2947    3257    3403    4549    2947 
   Size:        310     146    1146    3137

  Fragments arranged by size: 

                3137    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    491    1751     491 
   Size:       1260    3479
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    260     590     602     683    1444    1580    2564    2996    3743 
   Size:        330      12      81     761     136     984     432     747

Cuts at:   3743    4011     260 
   Size:        268     988

  Fragments arranged by size: 

                 988     984     761     747     432     330     268     136
                  81      12
 
EcoO109I rG'GnC_Cy

Cuts at:   1189    1190    1239    1455    1878    1189 
   Size:          1      49     216     423    4050

  Fragments arranged by size: 

                4050     423     216      49       1
 
EcoRI G'AATT_C

Cuts at:   1743    1743 
   Size:       4739
 
EcoRV GAT'ATC

Cuts at:    117     117 
   Size:       4739
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2758    2758 
   Size:       4739
 
FspI TGC'GCA

Cuts at:   2520    2520 
   Size:       4739
 
HaeII r_GCGC'y

Cuts at:   2173    2421    3443    4422    2173 
   Size:        248    1022     979    2490

  Fragments arranged by size: 

                2490    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    418     429     450     461     531     548     563     580     888 
   Size:         11      21      11      70      17      15      17     308

Cuts at:    888     920    1304    1336    1639    1671    2581    2611    2613 
   Size:         32     384      32     303      32     910      30       2

Cuts at:   2613    2643     418 
   Size:         30    2514

  Fragments arranged by size: 

                2514     910     384     308     303      70      32      32
                  32      30      30      21      17      17      15      11
                  11       2
 
HincII GTy'rAC

Cuts at:   1778    1778 
   Size:       4739
 
KasI G'GCGC_C

Cuts at:   2169    2417    2169 
   Size:        248    4491
 
KpnI G_GTAC'C

Cuts at:    679    1759     679 
   Size:       1080    3659
 
MluI A'CGCG_T

Cuts at:   4172    4172 
   Size:       4739
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    534    1414    3410    3594     534 
   Size:        880    1996     184    1679

  Fragments arranged by size: 

                1996    1679     880     184
 
MscI TGG'CCA

Cuts at:   2500    2500 
   Size:       4739
 
MslI CAynn'nnrTG

Cuts at:    233    1183    2728    2855     233 
   Size:        950    1545     127    2117

  Fragments arranged by size: 

                2117    1545     950     127
 
MspA1I CmG'CkG

Cuts at:   1013    1094    1850    2164    2524    3537    3782    1013 
   Size:         81     756     314     360    1013     245    1970

  Fragments arranged by size: 

                1970    1013     756     360     314     245      81
 
NaeI GCC'GGC

Cuts at:   1477    2920    1477 
   Size:       1443    3296
 
NarI GG'CG_CC

Cuts at:   2170    2418    2170 
   Size:        248    4491
 
NcoI C'CATG_G

Cuts at:    107    1184     107 
   Size:       1077    3662
 
NgoMIV G'CCGG_C

Cuts at:   1475    2918    1475 
   Size:       1443    3296
 
NspI r_CATG'y

Cuts at:    226    1792    2292    3199     226 
   Size:       1566     500     907    1766

  Fragments arranged by size: 

                1766    1566     907     500
 
PasI CC'CwG_GG

Cuts at:    535     670     535 
   Size:        135    4604
 
PciI A'CATG_T

Cuts at:   3195    3195 
   Size:       4739
 
PmeI GTTT'AAAC

Cuts at:   1738    1738 
   Size:       4739
 
PmlI CAC'GTG

Cuts at:    363     363 
   Size:       4739
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3878    3910    3878 
   Size:         32    4707
 
PpuMI rG'GwC_Cy

Cuts at:   1239    1455    1239 
   Size:        216    4523
 
PsiI TTA'TAA

Cuts at:    482     482 
   Size:       4739
 
PspOMI G'GGCC_C

Cuts at:   1189    1189 
   Size:       4739
 
PstI C_TGCA'G

Cuts at:   1786    2471    1786 
   Size:        685    4054
 
PvuI CG_AT'CG

Cuts at:    104     436     104 
   Size:        332    4407
 
PvuII CAG'CTG

Cuts at:   1013    1094    2164    2524    1013 
   Size:         81    1070     360    3228

  Fragments arranged by size: 

                3228    1070     360      81
 
RsrII CG'GwC_CG

Cuts at:   2934    2934 
   Size:       4739
 
SacI G_AGCT'C

Cuts at:    493    1753     493 
   Size:       1260    3479
 
SalI G'TCGA_C

Cuts at:   1776    1776 
   Size:       4739
 
SapI GCTCTTCn'nnn_

Cuts at:   2762    2972    2762 
   Size:        210    4529
 
SbfI CC_TGCA'GG

Cuts at:   1786    1786 
   Size:       4739
 
ScaI AGT'ACT

Cuts at:   4256    4256 
   Size:       4739
 
SexAI A'CCwGG_T

Cuts at:    474     474 
   Size:       4739
 
SfcI C'TryA_G

Cuts at:     33    1153    1480    1782    2467    3460    3651      33 
   Size:       1120     327     302     685     993     191    1121

  Fragments arranged by size: 

                1121    1120     993     685     327     302     191
 
SfoI GGC'GCC

Cuts at:   2171    2419    2171 
   Size:        248    4491
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4739
 
SmaI CCC'GGG

Cuts at:   1761    1761 
   Size:       4739
 
SmlI C'TyrA_G

Cuts at:   1894    2809    3301    3563    1894 
   Size:        915     492     262    3070

  Fragments arranged by size: 

                3070     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4739
 
SphI G_CATG'C

Cuts at:   1792    2292    1792 
   Size:        500    4239
 
SspI AAT'ATT

Cuts at:   1035    1035 
   Size:       4739
 
StyI C'CwwG_G

Cuts at:    107     787    1184    1520    1873    2227     107 
   Size:        680     397     336     353     354    2619

  Fragments arranged by size: 

                2619     680     397     354     353     336
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1308    2322    2455    2914    3123    1308 
   Size:       1014     133     459     209    2924

  Fragments arranged by size: 

                2924    1014     459     209     133
 
TatI w'GTAC_w

Cuts at:    225    1173    4254     225 
   Size:        948    3081     710

  Fragments arranged by size: 

                3081     948     710
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4256    4288    4256 
   Size:         32    4707
 
Tth111I GACn'n_nGTC

Cuts at:   2536    2536 
   Size:       4739
 
XbaI T'CTAG_A

Cuts at:   1770    1770 
   Size:       4739
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    769    1270    2234     769 
   Size:        501     964    3274

  Fragments arranged by size: 

                3274     964     501
 
XmaI C'CCGG_G

Cuts at:   1759    1759 
   Size:       4739
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AleI     AlfI     AloI     AscI    AvrII
     BaeI     BcgI    BfrBI     BglI    BmgBI     BmtI     BsgI    BsiWI
     BsmI    BsmBI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI    PflMI     PfoI
    PshAI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI
     SrfI     StuI     SwaI     XhoI     XmnI     ZraI
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