Restriction map for pF1KB9876
 (Linear) (Six-base) MAPSORT of: KIBB9876.seq  Check: 3179  from: 1  to: 1626



KIBB9876.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1012    1626 
   Size:       1012     614
 
Acc65I G'GTAC_C

Cuts at:      0     567    1626 
   Size:        567    1059
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     494     575    1336    1472    1626 
   Size:        152     342      81     761     136     154

  Fragments arranged by size: 

                 761     342     154     152     136      81
 
AflIII A'CryG_T

Cuts at:      0     254    1626 
   Size:        254    1372
 
AhdI GACnn_n'nnGTC

Cuts at:      0     335     437    1545    1626 
   Size:        335     102    1108      81

  Fragments arranged by size: 

                1108     335     102      81
 
ApaI G_GGCC'C

Cuts at:      0    1085    1626 
   Size:       1085     541
 
ApoI r'AATT_y

Cuts at:      0      45     159     210     961    1263    1626 
   Size:         45     114      51     751     302     363

  Fragments arranged by size: 

                 751     363     302     114      51      45
 
BanI G'GyrC_C

Cuts at:      0     567    1626 
   Size:        567    1059
 
BanII G_rGCy'C

Cuts at:      0     385    1085    1626 
   Size:        385     700     541

  Fragments arranged by size: 

                 700     541     385
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     347     593     883     998    1541    1579    1626 
   Size:        347     246     290     115     543      38      47

  Fragments arranged by size: 

                 543     347     290     246     115      47      38
 
BbvCI CC'TCA_GC

Cuts at:      0     901    1626 
   Size:        901     725
 
BglII A'GATC_T

Cuts at:      0      90    1626 
   Size:         90    1536
 
Bme1580I G_kGCm'C

Cuts at:      0    1085    1626 
   Size:       1085     541
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     398    1626 
   Size:        398    1228
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482    1626 
   Size:        482    1144
 
Bpu10I CC'TnA_GC

Cuts at:      0     901    1626 
   Size:        901     725
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     462    1626 
   Size:        462    1164
 
BsaAI yAC'GTr

Cuts at:      0     255    1626 
   Size:        255    1371
 
BsaBI GATnn'nnATC

Cuts at:      0      83      89    1626 
   Size:         83       6    1537

  Fragments arranged by size: 

                1537      83       6
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     440     470    1626 
   Size:        440      30    1156

  Fragments arranged by size: 

                1156     440      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     479    1626 
   Size:         85     394    1147

  Fragments arranged by size: 

                1147     394      85
 
BseYI C'CCAG_C

Cuts at:      0     986    1626 
   Size:        986     640
 
BsiEI CG_ry'CG

Cuts at:      0     328    1626 
   Size:        328    1298
 
BsiHKAI G_wGCw'C

Cuts at:      0     385    1406    1626 
   Size:        385    1021     220

  Fragments arranged by size: 

                1021     385     220
 
Bsp1286I G_dGCh'C

Cuts at:      0     385    1085    1406    1626 
   Size:        385     700     321     220

  Fragments arranged by size: 

                 700     385     321     220
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1012    1626 
   Size:       1012     614
 
BsrDI GCAATG_nn'

Cuts at:      0     758    1626 
   Size:        758     868
 
BsrFI r'CCGG_y

Cuts at:      0     303    1019    1367    1626 
   Size:        303     716     348     259

  Fragments arranged by size: 

                 716     348     303     259
 
BsrGI T'GTAC_A

Cuts at:      0     117    1065    1626 
   Size:        117     948     561

  Fragments arranged by size: 

                 948     561     117
 
BssSI C'ACGA_G

Cuts at:      0    1460    1626 
   Size:       1460     166
 
BstYI r'GATC_y

Cuts at:      0      84      90     635     720    1626 
   Size:         84       6     545      85     906

  Fragments arranged by size: 

                 906     545      85      84       6
 
BtgI C'CryG_G

Cuts at:      0    1076    1626 
   Size:       1076     550
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1056    1626 
   Size:       1056     570
 
BtsI GCAGTG_nn'

Cuts at:      0    1011    1626 
   Size:       1011     615
 
DraI TTT'AAA

Cuts at:      0     894    1431    1626 
   Size:        894     537     195

  Fragments arranged by size: 

                 894     537     195
 
EcoICRI GAG'CTC

Cuts at:      0     383    1626 
   Size:        383    1243
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     482     494     575    1336    1472    1626 
   Size:        152     330      12      81     761     136     154

  Fragments arranged by size: 

                 761     330     154     152     136      81      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1081    1082    1131    1347    1626 
   Size:       1081       1      49     216     279

  Fragments arranged by size: 

                1081     279     216      49       1
 
EcoRV GAT'ATC

Cuts at:      0       9    1626 
   Size:          9    1617
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     321     342     353     423     440     455     472 
   Size:        310      11      21      11      70      17      15      17

Cuts at:    472     780     812    1196    1228    1531    1563    1626 
   Size:        308      32     384      32     303      32      63

  Fragments arranged by size: 

                 384     310     308     303      70      63      32      32
                  32      21      17      17      15      11      11
 
KpnI G_GTAC'C

Cuts at:      0     571    1626 
   Size:        571    1055
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     426    1306    1626 
   Size:        426     880     320

  Fragments arranged by size: 

                 880     426     320
 
MslI CAynn'nnrTG

Cuts at:      0     125    1075    1626 
   Size:        125     950     551

  Fragments arranged by size: 

                 950     551     125
 
MspA1I CmG'CkG

Cuts at:      0     905     986    1626 
   Size:        905      81     640

  Fragments arranged by size: 

                 905     640      81
 
NaeI GCC'GGC

Cuts at:      0    1369    1626 
   Size:       1369     257
 
NcoI C'CATG_G

Cuts at:      0    1076    1626 
   Size:       1076     550
 
NgoMIV G'CCGG_C

Cuts at:      0    1367    1626 
   Size:       1367     259
 
NspI r_CATG'y

Cuts at:      0     118    1626 
   Size:        118    1508
 
PasI CC'CwG_GG

Cuts at:      0     427     562    1626 
   Size:        427     135    1064

  Fragments arranged by size: 

                1064     427     135
 
PmlI CAC'GTG

Cuts at:      0     255    1626 
   Size:        255    1371
 
PpuMI rG'GwC_Cy

Cuts at:      0    1131    1347    1626 
   Size:       1131     216     279

  Fragments arranged by size: 

                1131     279     216
 
PsiI TTA'TAA

Cuts at:      0     374    1626 
   Size:        374    1252
 
PspOMI G'GGCC_C

Cuts at:      0    1081    1626 
   Size:       1081     545
 
PvuI CG_AT'CG

Cuts at:      0     328    1626 
   Size:        328    1298
 
PvuII CAG'CTG

Cuts at:      0     905     986    1626 
   Size:        905      81     640

  Fragments arranged by size: 

                 905     640      81
 
SacI G_AGCT'C

Cuts at:      0     385    1626 
   Size:        385    1241
 
SexAI A'CCwGG_T

Cuts at:      0     366    1626 
   Size:        366    1260
 
SfcI C'TryA_G

Cuts at:      0    1045    1372    1626 
   Size:       1045     327     254

  Fragments arranged by size: 

                1045     327     254
 
SspI AAT'ATT

Cuts at:      0     927    1626 
   Size:        927     699
 
StyI C'CwwG_G

Cuts at:      0     679    1076    1412    1626 
   Size:        679     397     336     214

  Fragments arranged by size: 

                 679     397     336     214
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1200    1626 
   Size:       1200     426
 
TatI w'GTAC_w

Cuts at:      0     117    1065    1626 
   Size:        117     948     561

  Fragments arranged by size: 

                 948     561     117
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     661    1162    1626 
   Size:        661     501     464

  Fragments arranged by size: 

                 661     501     464
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AhdI     ApaI     ApoI     BanI
    BanII     BbsI    BbvCI    BglII Bme1580I     BmrI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI    BssSI    BstYI     BtgI
    BtgZI     BtsI     DraI  EcoICRI  Eco57MI EcoO109I    EcoRV    Hin4I
     KpnI     MmeI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI     PmlI    PpuMI     PsiI   PspOMI     PvuI    PvuII     SacI
    SexAI     SfcI     SspI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AleI     AlfI
     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmtI     BplI    BpuEI    BsaHI    BsaWI     BsgI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI   BssHII   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI    EcoNI    EcoRI     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MscI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI
    PspXI     PsrI     PstI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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