Restriction map for pF1KE1054
 (Circular) (Six-base) MAPSORT of: pF1KE1054.seq  Check: 8063  from: 1  to: 4724



pF1KE1054  4724 bp

 With 182 enzymes: SgfI * 

                        October 31, 2008 11:51  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    760     760 
   Size:       4724
 
AatII G_ACGT'C

Cuts at:   1373    1373 
   Size:       4724
 
AccI GT'mk_AC

Cuts at:   1762    1762 
   Size:       4724
 
Acc65I G'GTAC_C

Cuts at:   1740    1740 
   Size:       4724
 
AclI AA'CG_TT

Cuts at:   4564    4564 
   Size:       4724
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1036    2549    2981    3728    3996    1036 
   Size:       1513     432     747     268    1764

  Fragments arranged by size: 

                1764    1513     747     432     268
 
AfeI AGC'GCT

Cuts at:   1049    1049 
   Size:       4724
 
AflII C'TTAA_G

Cuts at:    855    1468     855 
   Size:        613    4111
 
AflIII A'CryG_T

Cuts at:   1110    2076    3180    4157    1110 
   Size:        966    1104     977    1677

  Fragments arranged by size: 

                1677    1104     977     966
 
AgeI A'CCGG_T

Cuts at:   1384    3112    1384 
   Size:       1728    2996
 
AlwNI CAG_nnn'CTG

Cuts at:   1606    2029    3596    1606 
   Size:        423    1567    2734

  Fragments arranged by size: 

                2734    1567     423
 
ApaLI G'TGCA_C

Cuts at:   3494    3494 
   Size:       4724
 
ApoI r'AATT_y

Cuts at:    317     418    1728    4077    4333     317 
   Size:        101    1310    2349     256     708

  Fragments arranged by size: 

                2349    1310     708     256     101
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4724
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4724
 
AvaI C'yCGr_G

Cuts at:   1744    1744 
   Size:       4724
 
BamHI G'GATC_C

Cuts at:   1749    1749 
   Size:       4724
 
BanI G'GyrC_C

Cuts at:   1740    2154    2402    2437    1740 
   Size:        414     248      35    4027

  Fragments arranged by size: 

                4027     414     248      35
 
BanII G_rGCy'C

Cuts at:    853    1738    2768     853 
   Size:        885    1030    2809

  Fragments arranged by size: 

                2809    1030     885
 
BbeI G_GCGC'C

Cuts at:   2158    2406    2158 
   Size:        248    4476
 
BciVI GTATCCnnnnn_n'

Cuts at:   2622    3383    4724    2622 
   Size:        761    1341    2622

  Fragments arranged by size: 

                2622    1341     761
 
BclI T'GATC_A

Cuts at:   2244    2244 
   Size:       4724
 
BglII A'GATC_T

Cuts at:    896    2239    4276     896 
   Size:       1343    2037    1344

  Fragments arranged by size: 

                2037    1344    1343
 
BlpI GC'TnA_GC

Cuts at:   1836    1836 
   Size:       4724
 
Bme1580I G_kGCm'C

Cuts at:   2349    2442    3498    2349 
   Size:         93    1056    3575

  Fragments arranged by size: 

                3575    1056      93
 
BmgBI CAC'GTC

Cuts at:    209     209 
   Size:       4724
 
BmrI ACTGGGnnnn_n'

Cuts at:   1644    1936    2017    2352    4496    1644 
   Size:        292      81     335    2144    1872

  Fragments arranged by size: 

                2144    1872     335     292      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1592    3091    1592 
   Size:       1499    3225
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    204    1129     204 
   Size:        925    3799
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1663    1900    2779    3271    3569    1663 
   Size:        237     879     492     298    2818

  Fragments arranged by size: 

                2818     879     492     298     237
 
BsaI GGTCTCn'nnnn_

Cuts at:   4428    4428 
   Size:       4724
 
BsaAI yAC'GTr

Cuts at:   2077    2077 
   Size:       4724
 
BsaBI GATnn'nnATC

Cuts at:      6    2238    4275       6 
   Size:       2232    2037     455

  Fragments arranged by size: 

                2232    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1370    2155    2403    3105    4601    1370 
   Size:        785     248     702    1496    1493

  Fragments arranged by size: 

                1496    1493     785     702     248
 
BsaWI w'CCGG_w

Cuts at:    777    1384    1913    2434    3112    3386    3533    3944     777 
   Size:        607     529     521     678     274     147     411    1557

  Fragments arranged by size: 

                1557     678     607     529     521     411     274     147
            
 
BseYI C'CCAG_C

Cuts at:   1506    2149    2907    3484    3980    1506 
   Size:        643     758     577     496    2250

  Fragments arranged by size: 

                2250     758     643     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    261     261 
   Size:       4724
 
BsiEI CG_ry'CG

Cuts at:    104    1389    2266    2312    3520     104 
   Size:       1285     877      46    1208    1308

  Fragments arranged by size: 

                1308    1285    1208     877      46
 
BsiHKAI G_wGCw'C

Cuts at:   1738    2516    2706    3498    1738 
   Size:        778     190     792    2964

  Fragments arranged by size: 

                2964     792     778     190
 
BsmI GAATG_Cn'

Cuts at:   1013    1013 
   Size:       4724
 
Bsp1286I G_dGCh'C

Cuts at:    853    1738    2349    2442    2516    2706    2768    3498     853 
   Size:        885     611      93      74     190      62     730    2079

  Fragments arranged by size: 

                2079     885     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3944    3944 
   Size:       4724
 
BspHI T'CATG_A

Cuts at:   3900    3900 
   Size:       4724
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    464     680     760    1774    1988    2290    2671     464 
   Size:        216      80    1014     214     302     381    2517

  Fragments arranged by size: 

                2517    1014     381     302     216     214      80
 
BsrBI CCG'CTC

Cuts at:     50    3016    3070    4554    4719      50 
   Size:       2966      54    1484     165      55

  Fragments arranged by size: 

                2966    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1530    2636    1530 
   Size:       1106    3618
 
BsrFI r'CCGG_y

Cuts at:   1384    2722    2903    3112    1384 
   Size:       1338     181     209    2996

  Fragments arranged by size: 

                2996    1338     209     181
 
BssSI C'ACGA_G

Cuts at:   2995    3353    2995 
   Size:        358    4366
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2101    2101 
   Size:       4724
 
BstBI TT'CG_AA

Cuts at:   3085    3085 
   Size:       4724
 
BstEII G'GTnAC_C

Cuts at:    805     805 
   Size:       4724
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1657    1691    1657 
   Size:         34    4690
 
BstYI r'GATC_y

Cuts at:      1     233     297     896    1628    1749    2216    2239    2574 
   Size:        232      64     599     732     121     467      23     335

Cuts at:   2574    2820    3832    3918    3930    4276       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     732     599     467     449     346     335     246
                 232     121      86      64      23      12
 
BtgI C'CryG_G

Cuts at:    140    1134     140 
   Size:        994    3730
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    309    2674    2855    3018     309 
   Size:       2365     181     163    2015

  Fragments arranged by size: 

                2365    2015     181     163
 
BtsI GCAGTG_nn'

Cuts at:    228    1967     228 
   Size:       1739    2985
 
DraI TTT'AAA

Cuts at:   1723    1723 
   Size:       4724
 
DrdI GACnn_nn'nnGTC

Cuts at:    495    2430    3288     495 
   Size:       1935     858    1931

  Fragments arranged by size: 

                1935    1931     858
 
EaeI y'GGCC_r

Cuts at:    710    2309    2483    2874    2901    4246     710 
   Size:       1599     174     391      27    1345    1188

  Fragments arranged by size: 

                1599    1345    1188     391     174      27
 
EagI C'GGCC_G

Cuts at:   2309    2309 
   Size:       4724
 
EarI CTCTTCn'nnn_

Cuts at:   2747    2957    2747 
   Size:        210    4514
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    925    2932    3242    3388    4534     925 
   Size:       2007     310     146    1146    1115

  Fragments arranged by size: 

                2007    1146    1115     310     146
 
EcoICRI GAG'CTC

Cuts at:   1736    1736 
   Size:       4724
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    204    1036    1129    2549    2981    3728    3996     204 
   Size:        832      93    1420     432     747     268     932

  Fragments arranged by size: 

                1420     932     832     747     432     268      93
 
EcoNI CCTnn'n_nnAGG

Cuts at:    120     120 
   Size:       4724
 
EcoO109I rG'GnC_Cy

Cuts at:   1863    1863 
   Size:       4724
 
EcoRI G'AATT_C

Cuts at:   1728    1728 
   Size:       4724
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2743    2743 
   Size:       4724
 
FspI TGC'GCA

Cuts at:   2505    2505 
   Size:       4724
 
HaeII r_GCGC'y

Cuts at:   1051    2158    2406    3428    4407    1051 
   Size:       1107     248    1022     979    1368

  Fragments arranged by size: 

                1368    1107    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    573     589     605     621    1218    1250    1519    1551    2566 
   Size:         16      16      16     597      32     269      32    1015

Cuts at:   2566    2596    2598    2628     573 
   Size:         30       2      30    2669

  Fragments arranged by size: 

                2669    1015     597     269      32      32      30      30
                  16      16      16       2
 
HincII GTy'rAC

Cuts at:    945    1763     945 
   Size:        818    3906
 
HindIII A'AGCT_T

Cuts at:    371     371 
   Size:       4724
 
KasI G'GCGC_C

Cuts at:   2154    2402    2154 
   Size:        248    4476
 
KpnI G_GTAC'C

Cuts at:   1744    1744 
   Size:       4724
 
MfeI C'AATT_G

Cuts at:    398     684     398 
   Size:        286    4438
 
MluI A'CGCG_T

Cuts at:   4157    4157 
   Size:       4724
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1015    3395    3579    1015 
   Size:       2380     184    2160

  Fragments arranged by size: 

                2380    2160     184
 
MscI TGG'CCA

Cuts at:   2485    2485 
   Size:       4724
 
MslI CAynn'nnrTG

Cuts at:    558     827    1165    2713    2840     558 
   Size:        269     338    1548     127    2442

  Fragments arranged by size: 

                2442    1548     338     269     127
 
MspA1I CmG'CkG

Cuts at:   1149    1835    2149    2509    3522    3767    1149 
   Size:        686     314     360    1013     245    2106

  Fragments arranged by size: 

                2106    1013     686     360     314     245
 
NaeI GCC'GGC

Cuts at:   2905    2905 
   Size:       4724
 
NarI GG'CG_CC

Cuts at:   2155    2403    2155 
   Size:        248    4476
 
NcoI C'CATG_G

Cuts at:    140     140 
   Size:       4724
 
NdeI CA'TA_TG

Cuts at:    824     824 
   Size:       4724
 
NgoMIV G'CCGG_C

Cuts at:   2903    2903 
   Size:       4724
 
NspI r_CATG'y

Cuts at:    563    1114    1777    2277    3184     563 
   Size:        551     663     500     907    2103

  Fragments arranged by size: 

                2103     907     663     551     500
 
PciI A'CATG_T

Cuts at:   1110    3180    1110 
   Size:       2070    2654
 
PfoI T'CCnGG_A

Cuts at:    115     115 
   Size:       4724
 
PmeI GTTT'AAAC

Cuts at:   1723    1723 
   Size:       4724
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3863    3895    3863 
   Size:         32    4692
 
PstI C_TGCA'G

Cuts at:    461     677    1317    1771    2456     461 
   Size:        216     640     454     685    2729

  Fragments arranged by size: 

                2729     685     640     454     216
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4724
 
PvuII CAG'CTG

Cuts at:   1149    2149    2509    1149 
   Size:       1000     360    3364

  Fragments arranged by size: 

                3364    1000     360
 
RsrII CG'GwC_CG

Cuts at:   2919    2919 
   Size:       4724
 
SacI G_AGCT'C

Cuts at:   1738    1738 
   Size:       4724
 
SalI G'TCGA_C

Cuts at:   1761    1761 
   Size:       4724
 
SapI GCTCTTCn'nnn_

Cuts at:   2747    2957    2747 
   Size:        210    4514
 
SbfI CC_TGCA'GG

Cuts at:   1771    1771 
   Size:       4724
 
ScaI AGT'ACT

Cuts at:   1654    4241    1654 
   Size:       2587    2137
 
SexAI A'CCwGG_T

Cuts at:    809     809 
   Size:       4724
 
SfcI C'TryA_G

Cuts at:     33     457     673     759    1313    1767    2452    3445    3636 
   Size:        424     216      86     554     454     685     993     191

Cuts at:   3636      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     554     454     424     216     191
                  86
 
SfoI GGC'GCC

Cuts at:   2156    2404    2156 
   Size:        248    4476
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4724
 
SmaI CCC'GGG

Cuts at:   1746    1746 
   Size:       4724
 
SmlI C'TyrA_G

Cuts at:    855    1468    1678    1879    2794    3286    3548     855 
   Size:        613     210     201     915     492     262    2031

  Fragments arranged by size: 

                2031     915     613     492     262     210     201
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4724
 
SphI G_CATG'C

Cuts at:   1777    2277    1777 
   Size:        500    4224
 
SspI AAT'ATT

Cuts at:    657    1076     657 
   Size:        419    4305
 
StyI C'CwwG_G

Cuts at:    140    1858    2212     140 
   Size:       1718     354    2652

  Fragments arranged by size: 

                2652    1718     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1392    2307    2440    2899    3108    1392 
   Size:        915     133     459     209    3008

  Fragments arranged by size: 

                3008     915     459     209     133
 
TatI w'GTAC_w

Cuts at:    629    1099    1652    4239     629 
   Size:        470     553    2587    1114

  Fragments arranged by size: 

                2587    1114     553     470
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4241    4273    4241 
   Size:         32    4692
 
Tth111I GACn'n_nGTC

Cuts at:   2521    2521 
   Size:       4724
 
XbaI T'CTAG_A

Cuts at:   1755    1755 
   Size:       4724
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1052    2219    1052 
   Size:       1167    3557
 
XmaI C'CCGG_G

Cuts at:   1744    1744 
   Size:       4724
 
XmnI GAAnn'nnTTC

Cuts at:    508     508 
   Size:       4724
 
ZraI GAC'GTC

Cuts at:   1371    1371 
   Size:       4724
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI    BciVI     BclI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI     PciI
     PfoI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AhdI     AleI     AlfI     AloI     ApaI     AscI    AvrII     BaeI
     BbsI    BbvCI     BcgI    BfrBI     BglI     BmtI   Bpu10I    BsaXI
    BseRI    BsiWI    BsmBI    BsrGI   BssHII  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoRV     FseI    FspAI     HpaI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI
    SnaBI     SrfI     StuI     SwaI     XhoI
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