Restriction map for
pF1KB7752
(Circular) (Six-base) MAPSORT of: pF1KB7752.seq Check: 5968 from: 1 to: 4583
pF1KB7752 4583 bp
With 182 enzymes: SgfI *
October 14, 2008 16:51 ..
AccI GT'mk_AC
Cuts at: 1621 1621
Size: 4583
Acc65I G'GTAC_C
Cuts at: 1599 1599
Size: 4583
AclI AA'CG_TT
Cuts at: 4423 4423
Size: 4583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 735 880 1384 2408 2840 3587 3855 614
Size: 121 145 504 1024 432 747 268 1342
Fragments arranged by size:
1342 1024 747 504 432 268 145 121
AflIII A'CryG_T
Cuts at: 1935 3039 4016 1935
Size: 1104 977 2502
Fragments arranged by size:
2502 1104 977
AgeI A'CCGG_T
Cuts at: 2971 2971
Size: 4583
AleI CACnn'nnGTG
Cuts at: 790 1051 790
Size: 261 4322
AlwNI CAG_nnn'CTG
Cuts at: 395 1888 3455 395
Size: 1493 1567 1523
Fragments arranged by size:
1567 1523 1493
ApaLI G'TGCA_C
Cuts at: 3353 3353
Size: 4583
ApoI r'AATT_y
Cuts at: 676 1460 1587 3936 4192 676
Size: 784 127 2349 256 1067
Fragments arranged by size:
2349 1067 784 256 127
AseI AT'TA_AT
Cuts at: 20 20
Size: 4583
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
AvaI C'yCGr_G
Cuts at: 1095 1472 1515 1603 1095
Size: 377 43 88 4075
Fragments arranged by size:
4075 377 88 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 291 324 291
Size: 33 4550
BamHI G'GATC_C
Cuts at: 1608 1608
Size: 4583
BanI G'GyrC_C
Cuts at: 1599 2013 2261 2296 1599
Size: 414 248 35 3886
Fragments arranged by size:
3886 414 248 35
BanII G_rGCy'C
Cuts at: 587 1414 1423 1522 1597 2627 587
Size: 827 9 99 75 1030 2543
Fragments arranged by size:
2543 1030 827 99 75 9
BbeI G_GCGC'C
Cuts at: 2017 2265 2017
Size: 248 4335
BbsI GAAGACnn'nnnn_
Cuts at: 848 1496 848
Size: 648 3935
BciVI GTATCCnnnnn_n'
Cuts at: 2481 3242 4583 2481
Size: 761 1341 2481
Fragments arranged by size:
2481 1341 761
BclI T'GATC_A
Cuts at: 2103 2103
Size: 4583
BfrBI ATG'CAT
Cuts at: 1323 1323
Size: 4583
BglI GCCn_nnn'nGGC
Cuts at: 625 1274 625
Size: 649 3934
BglII A'GATC_T
Cuts at: 2098 4135 2098
Size: 2037 2546
BlpI GC'TnA_GC
Cuts at: 1695 1695
Size: 4583
Bme1580I G_kGCm'C
Cuts at: 2208 2301 3357 2208
Size: 93 1056 3434
Fragments arranged by size:
3434 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1021 1294 1795 1876 2211 4355 1021
Size: 273 501 81 335 2144 1249
Fragments arranged by size:
2144 1249 501 335 273 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1527 2950 1527
Size: 1423 3160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1459 1459
Size: 4583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1375 1759 2638 3130 3428 952
Size: 423 384 879 492 298 2107
Fragments arranged by size:
2107 879 492 423 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 4287 4287
Size: 4583
BsaAI yAC'GTr
Cuts at: 1936 1936
Size: 4583
BsaBI GATnn'nnATC
Cuts at: 6 2097 4134 6
Size: 2091 2037 455
Fragments arranged by size:
2091 2037 455
BsaHI Gr'CG_yC
Cuts at: 998 1289 2014 2262 2964 4460 998
Size: 291 725 248 702 1496 1121
Fragments arranged by size:
1496 1121 725 702 291 248
BsaWI w'CCGG_w
Cuts at: 1536 1772 2293 2971 3245 3392 3803 1536
Size: 236 521 678 274 147 411 2316
Fragments arranged by size:
2316 678 521 411 274 236 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 124 154 719 749 868 898 124
Size: 30 565 30 119 30 3809
Fragments arranged by size:
3809 565 119 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 565 1512 1543 166
Size: 399 947 31 3206
Fragments arranged by size:
3206 947 399 31
BseYI C'CCAG_C
Cuts at: 2008 2766 3343 3839 2008
Size: 758 577 496 2752
Fragments arranged by size:
2752 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 1249
Size: 4583
BsiEI CG_ry'CG
Cuts at: 104 1273 2125 2171 3379 104
Size: 1169 852 46 1208 1308
Fragments arranged by size:
1308 1208 1169 852 46
BsiHKAI G_wGCw'C
Cuts at: 1160 1522 1597 2375 2565 3357 1160
Size: 362 75 778 190 792 2386
Fragments arranged by size:
2386 792 778 362 190 75
BsmI GAATG_Cn'
Cuts at: 1325 1325
Size: 4583
BsmBI CGTCTCn'nnnn_
Cuts at: 1438 1438
Size: 4583
Bsp1286I G_dGCh'C
Cuts at: 587 1160 1414 1423 1522 1597 2208 2301 2375
Size: 573 254 9 99 75 611 93 74
Cuts at: 2375 2565 2627 3357 587
Size: 190 62 730 1813
Fragments arranged by size:
1813 730 611 573 254 190 99 93
75 74 62 9
BspEI T'CCGG_A
Cuts at: 3803 3803
Size: 4583
BspHI T'CATG_A
Cuts at: 3759 3759
Size: 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1151 1332 1633 1847 2149 2530 1151
Size: 181 301 214 302 381 3204
Fragments arranged by size:
3204 381 302 301 214 181
BsrBI CCG'CTC
Cuts at: 50 537 1094 1477 2875 2929 4413 4578 50
Size: 487 557 383 1398 54 1484 165 55
Fragments arranged by size:
1484 1398 557 487 383 165 55 54
BsrDI GCAATG_nn'
Cuts at: 614 1569 2495 614
Size: 955 926 2702
Fragments arranged by size:
2702 955 926
BsrFI r'CCGG_y
Cuts at: 1268 1392 2581 2762 2971 1268
Size: 124 1189 181 209 2880
Fragments arranged by size:
2880 1189 209 181 124
BsrGI T'GTAC_A
Cuts at: 1244 1244
Size: 4583
BssSI C'ACGA_G
Cuts at: 2854 3212 2854
Size: 358 4225
BstAPI GCAn_nnn'nTGC
Cuts at: 425 1071 1564 1960 425
Size: 646 493 396 3048
Fragments arranged by size:
3048 646 493 396
BstBI TT'CG_AA
Cuts at: 2944 2944
Size: 4583
BstEII G'GTnAC_C
Cuts at: 167 167
Size: 4583
BstXI CCAn_nnnn'nTGG
Cuts at: 477 477
Size: 4583
BstYI r'GATC_y
Cuts at: 1 603 1608 2075 2098 2433 2679 3691 3777
Size: 602 1005 467 23 335 246 1012 86
Cuts at: 3777 3789 4135 1
Size: 12 346 449
Fragments arranged by size:
1012 1005 602 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 458 470 107
Size: 351 12 4220
Fragments arranged by size:
4220 351 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 438 616 2533 2714 2877 438
Size: 178 1917 181 163 2144
Fragments arranged by size:
2144 1917 181 178 163
BtsI GCAGTG_nn'
Cuts at: 268 565 1506 1826 268
Size: 297 941 320 3025
Fragments arranged by size:
3025 941 320 297
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 110 145 110
Size: 35 4548
DraI TTT'AAA
Cuts at: 1582 1582
Size: 4583
DrdI GACnn_nn'nnGTC
Cuts at: 2289 3147 2289
Size: 858 3725
EaeI y'GGCC_r
Cuts at: 198 467 503 696 779 1270 2168 2342 2733
Size: 269 36 193 83 491 898 174 391
Cuts at: 2733 2760 4105 198
Size: 27 1345 676
Fragments arranged by size:
1345 898 676 491 391 269 193 174
83 36 27
EagI C'GGCC_G
Cuts at: 1270 2168 1270
Size: 898 3685
EarI CTCTTCn'nnn_
Cuts at: 590 1120 1411 2606 2816 590
Size: 530 291 1195 210 2357
Fragments arranged by size:
2357 1195 530 291 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 558 687 1522 2791 3101 3247 4393 558
Size: 129 835 1269 310 146 1146 748
Fragments arranged by size:
1269 1146 835 748 310 146 129
EcoICRI GAG'CTC
Cuts at: 1520 1595 1520
Size: 75 4508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 735 880 1384 1459 2408 2840 3587 3855
Size: 121 145 504 75 949 432 747 268
Cuts at: 3855 614
Size: 1342
Fragments arranged by size:
1342 949 747 504 432 268 145 121
75
EcoNI CCTnn'n_nnAGG
Cuts at: 859 859
Size: 4583
EcoO109I rG'GnC_Cy
Cuts at: 1551 1722 1551
Size: 171 4412
EcoRI G'AATT_C
Cuts at: 1460 1587 1460
Size: 127 4456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 351 2602 351
Size: 2251 2332
FseI GG_CCGG'CC
Cuts at: 1272 1272
Size: 4583
FspI TGC'GCA
Cuts at: 2364 2364
Size: 4583
HaeII r_GCGC'y
Cuts at: 2017 2265 3287 4266 2017
Size: 248 1022 979 2334
Fragments arranged by size:
2334 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 868 900 2425 2455 2457 2487 124
Size: 32 712 32 1525 30 2 30 2220
Fragments arranged by size:
2220 1525 712 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1085 1167 1622 1085
Size: 82 455 4046
Fragments arranged by size:
4046 455 82
KasI G'GCGC_C
Cuts at: 2013 2261 2013
Size: 248 4335
KpnI G_GTAC'C
Cuts at: 1603 1603
Size: 4583
MluI A'CGCG_T
Cuts at: 4016 4016
Size: 4583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1019 3254 3438 1019
Size: 2235 184 2164
Fragments arranged by size:
2235 2164 184
MscI TGG'CCA
Cuts at: 469 505 698 2344 469
Size: 36 193 1646 2708
Fragments arranged by size:
2708 1646 193 36
MslI CAynn'nnrTG
Cuts at: 457 469 790 1051 2572 2699 457
Size: 12 321 261 1521 127 2341
Fragments arranged by size:
2341 1521 321 261 127 12
MspA1I CmG'CkG
Cuts at: 305 392 432 1258 1694 2008 2368 3381 3626
Size: 87 40 826 436 314 360 1013 245
Cuts at: 3626 305
Size: 1262
Fragments arranged by size:
1262 1013 826 436 360 314 245 87
40
NaeI GCC'GGC
Cuts at: 1270 2764 1270
Size: 1494 3089
NarI GG'CG_CC
Cuts at: 2014 2262 2014
Size: 248 4335
NcoI C'CATG_G
Cuts at: 107 470 107
Size: 363 4220
NgoMIV G'CCGG_C
Cuts at: 1268 2762 1268
Size: 1494 3089
NsiI A_TGCA'T
Cuts at: 1325 1325
Size: 4583
NspI r_CATG'y
Cuts at: 225 1327 1636 2136 3043 225
Size: 1102 309 500 907 1765
Fragments arranged by size:
1765 1102 907 500 309
PasI CC'CwG_GG
Cuts at: 648 648
Size: 4583
PciI A'CATG_T
Cuts at: 3039 3039
Size: 4583
PfoI T'CCnGG_A
Cuts at: 1436 1436
Size: 4583
PmeI GTTT'AAAC
Cuts at: 1582 1582
Size: 4583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 4551
PpuMI rG'GwC_Cy
Cuts at: 1551 1551
Size: 4583
PstI C_TGCA'G
Cuts at: 572 1067 1630 2315 572
Size: 495 563 685 2840
Fragments arranged by size:
2840 685 563 495
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4583
PvuII CAG'CTG
Cuts at: 392 432 2008 2368 392
Size: 40 1576 360 2607
Fragments arranged by size:
2607 1576 360 40
RsrII CG'GwC_CG
Cuts at: 2778 2778
Size: 4583
SacI G_AGCT'C
Cuts at: 1522 1597 1522
Size: 75 4508
SalI G'TCGA_C
Cuts at: 1620 1620
Size: 4583
SapI GCTCTTCn'nnn_
Cuts at: 1411 2606 2816 1411
Size: 1195 210 3178
Fragments arranged by size:
3178 1195 210
SbfI CC_TGCA'GG
Cuts at: 1630 1630
Size: 4583
ScaI AGT'ACT
Cuts at: 4100 4100
Size: 4583
SfcI C'TryA_G
Cuts at: 33 568 1063 1626 2311 3304 3495 33
Size: 535 495 563 685 993 191 1121
Fragments arranged by size:
1121 993 685 563 535 495 191
SfoI GGC'GCC
Cuts at: 2015 2263 2015
Size: 248 4335
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
SmaI CCC'GGG
Cuts at: 1605 1605
Size: 4583
SmlI C'TyrA_G
Cuts at: 967 1354 1738 2653 3145 3407 967
Size: 387 384 915 492 262 2143
Fragments arranged by size:
2143 915 492 387 384 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4583
SphI G_CATG'C
Cuts at: 1636 2136 1636
Size: 500 4083
StyI C'CwwG_G
Cuts at: 107 470 1386 1717 2071 107
Size: 363 916 331 354 2619
Fragments arranged by size:
2619 916 363 354 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 720 2166 2299 2758 2967 720
Size: 1446 133 459 209 2336
Fragments arranged by size:
2336 1446 459 209 133
TatI w'GTAC_w
Cuts at: 1244 4098 1244
Size: 2854 1729
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 719 751 1274 1306 4100 4132 719
Size: 32 523 32 2794 32 1170
Fragments arranged by size:
2794 1170 523 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2380 2380
Size: 4583
XbaI T'CTAG_A
Cuts at: 483 1614 483
Size: 1131 3452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 789 1008 2078 789
Size: 219 1070 3294
Fragments arranged by size:
3294 1070 219
XmaI C'CCGG_G
Cuts at: 1603 1603
Size: 4583
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BmgBI BmtI Bpu10I BsiWI
BssHII BstZ17I Bsu36I ClaI DraIII EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI