Restriction map for
pF1KB6290
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AscI Asp718I
AspI AviII AvrII BalI BbeI
BbrPI BcgI BciVI BfrI BlnI
BsaAI BsaBI BsaHI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraI
DrdI EaeI EagI EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 1352 TCCGGA T^CCGGA
AflIII 2002 ACACGT A^C[GA][CT]GT
AhdI 2095 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1630 GTGCAC G^TGCAC
AocI 899 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2078 AGCGCT AGC^GCT
ApaLI 1630 GTGCAC G^TGCAC
AseI 2332 ATTAAT AT^TAAT
AsnI 2332 ATTAAT AT^TAAT
Asp700I 1992 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2095 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1854 GGATCC G^GATCC
BbsI 405 GTCTTC GAAGAC(2/6)
BclI 1677 TGATCA T^GATCA
BcnI 1796 CCCGG CC^[GC]GG
BfaI 1213 CTAG C^TAG
BglII 2319 AGATCT A^GATCT
BlpI 1687 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1687 GCTCAGC GC^T[ACGT]AGC
BpuAI 405 GTCTTC GAAGAC(2/6)
BsaI 1696 GGTCTC GGTCTC(1/5)
BsaWI 1352 TCCGGA [AT]^CCGG[AT]
BseAI 1352 TCCGGA T^CCGGA
BsgI 586 GTGCAG GTGCAG(16/14)
BsiEI 1785 CGGTCG CG[GA][CT]^CG
BsmI 1554 GAATGC GAATGC(1/-1)
BspEI 1352 TCCGGA T^CCGGA
BspHI 2084 TCATGA T^CATGA
BsrBI 452 CCGCTC CCGCTC(-3/-3)
BsrFI 1783 GCCGGT [GA]^CCGG[CT]
BstAPI 552 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 899 CCTCAGG CC^T[ACGT]AGG
CelII 1687 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1783 GCCGGT [GA]^CCGG[CT]
DraIII 302 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2095 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 330 CTCTTC CTCTTC(1/4)
Eco47III 2078 AGCGCT AGC^GCT
Eco81I 899 CCTCAGG CC^T[ACGT]AGG
FbaI 1677 TGATCA T^GATCA
HaeII 2078 AGCGCT [GA]GCGC^[CT]
HincII 805 GTCAAC GT[CT]^[GA]AC
HindII 805 GTCAAC GT[CT]^[GA]AC
Ksp632I 330 CTCTTC CTCTTC(1/4)
MaeI 1213 CTAG C^TAG
MroI 1352 TCCGGA T^CCGGA
NciI 1796 CCCGG CC^[GC]GG
NspI 1807 GCATGC [GA]CATG^[CT]
PshBI 2332 ATTAAT AT^TAAT
RcaI 2084 TCATGA T^CATGA
SacI 1685 GAGCTC GAGCT^C
SfiI 1230 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1807 GCATGC GCATG^C
SspI 508 AATATT AAT^ATT
XmnI 1992 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 1193 AAATTC [GA]^AATT[CT]
2053 AAATTT
AlwNI 422 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1544 CAGTCACTG
ApoI 1193 AAATTC [GA]^AATT[CT]
2053 AAATTT
AspHI 1630 GTGCAC G[AT]GC[AT]^C
1685 GAGCTC
AvaI 249 CCCGAG C^[CT]CG[GA]G
1848 CTCGGG
BanI 97 GGTGCC G^G[CT][GA]CC
307 GGTGCC
BbvCI 1207 CCTCAGC CCTCAGC(-5/-2)
1517 CCTCAGC
BsiHKAI 1630 GTGCAC G[AT]GC[AT]^C
1685 GAGCTC
BsmAI 424 GTCTC GTCTC(1/5)
1697 GTCTC
BsoBI 249 CCCGAG C^[CT]CG[GA]G
1848 CTCGGG
BstYI 1854 GGATCC [GA]^GATC[CT]
2319 AGATCT
CfoI 1941 GCGC GCG^C
2079 GCGC
FauI 1800 GCGGG CCCGC(4/6)
1929 GCGGG
HhaI 1941 GCGC GCG^C
2079 GCGC
HinP1I 1941 GCGC G^CGC
2079 GCGC
MflI 1854 GGATCC [GA]^GATC[CT]
2319 AGATCT
MseI 1595 TTAA T^TAA
2333 TTAA
PpuMI 868 GGGTCCC [GA]G^G[AT]CC[CT]
1792 AGGACCC
SexAI 621 ACCAGGT A^CC[AT]GGT
1975 ACCTGGT
SmlI 1052 CTCAAG C^T[CT][GA]AG
1280 CTCAAG
TaqI 114 TCGA T^CGA
651 TCGA
TfiI 414 GAATC G^A[AT]TC
1622 GAATC
Tru9I 1595 TTAA T^TAA
2333 TTAA
TthHB8I 114 TCGA T^CGA
651 TCGA
XhoII 1854 GGATCC [GA]^GATC[CT]
2319 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaII BanII BbvI BglI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TseI Tsp45I Tsp509I
TspRI Van91I XcmI