Restriction map for pF1KB6290
 (Circular) (Six-base) MAPSORT of: pF1KB6290.seq  Check: 597  from: 1  to: 5468



pF1KB6290  5468 bp

 With 182 enzymes: SgfI * 

                        September 25, 2008 15:52  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1837    1930    2042    2409    1837 
   Size:         93     112     367    4896

  Fragments arranged by size: 

                4896     367     112      93
 
AccI GT'mk_AC

Cuts at:   2506    2506 
   Size:       5468
 
Acc65I G'GTAC_C

Cuts at:   2484    2484 
   Size:       5468
 
AclI AA'CG_TT

Cuts at:   5308    5308 
   Size:       5468
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1411    1709    2312    3293    3725    4472    4740    1411 
   Size:        298     603     981     432     747     268    2139

  Fragments arranged by size: 

                2139     981     747     603     432     298     268
 
AfeI AGC'GCT

Cuts at:   2188    2188 
   Size:       5468
 
AflIII A'CryG_T

Cuts at:   2110    2820    3924    4901    2110 
   Size:        710    1104     977    2677

  Fragments arranged by size: 

                2677    1104     977     710
 
AgeI A'CCGG_T

Cuts at:   3856    3856 
   Size:       5468
 
AhdI GACnn_n'nnGTC

Cuts at:   2208    2208 
   Size:       5468
 
AleI CACnn'nnGTG

Cuts at:   2150    2150 
   Size:       5468
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    324     692    1938     324 
   Size:        368    1246    3854

  Fragments arranged by size: 

                3854    1246     368
 
AlwNI CAG_nnn'CTG

Cuts at:    535    1657    2773    4340     535 
   Size:       1122    1116    1567    1663

  Fragments arranged by size: 

                1663    1567    1122    1116
 
ApaI G_GGCC'C

Cuts at:    358     690    1481     358 
   Size:        332     791    4345

  Fragments arranged by size: 

                4345     791     332
 
ApaLI G'TGCA_C

Cuts at:   1738    4238    1738 
   Size:       2500    2968
 
ApoI r'AATT_y

Cuts at:   1301    2161    2472    4821    5077    1301 
   Size:        860     311    2349     256    1692

  Fragments arranged by size: 

                2349    1692     860     311     256
 
AseI AT'TA_AT

Cuts at:     20    2441      20 
   Size:       2421    3047
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5468
 
AvaI C'yCGr_G

Cuts at:    357    1956    2488     357 
   Size:       1599     532    3337

  Fragments arranged by size: 

                3337    1599     532
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    857     890     857 
   Size:         33    5435
 
BamHI G'GATC_C

Cuts at:   1962    2493    1962 
   Size:        531    4937
 
BanI G'GyrC_C

Cuts at:    205     415    2484    2898    3146    3181     205 
   Size:        210    2069     414     248      35    2492

  Fragments arranged by size: 

                2492    2069     414     248     210      35
 
BanII G_rGCy'C

Cuts at:    358     690    1481    1797    1957    2482    3512     358 
   Size:        332     791     316     160     525    1030    2314

  Fragments arranged by size: 

                2314    1030     791     525     332     316     160
 
BbeI G_GCGC'C

Cuts at:   2902    3150    2902 
   Size:        248    5220
 
BbsI GAAGACnn'nnnn_

Cuts at:    506     506 
   Size:       5468
 
BbvCI CC'TCA_GC

Cuts at:   1316    1626    1316 
   Size:        310    5158
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2442    2476    2442 
   Size:         34    5434
 
BciVI GTATCCnnnnn_n'

Cuts at:   3366    4127    5468    3366 
   Size:        761    1341    3366

  Fragments arranged by size: 

                3366    1341     761
 
BclI T'GATC_A

Cuts at:   1785    2988    1785 
   Size:       1203    4265
 
BglI GCCn_nnn'nGGC

Cuts at:    685    1345    2362     685 
   Size:        660    1017    3791

  Fragments arranged by size: 

                3791    1017     660
 
BglII A'GATC_T

Cuts at:   2427    2983    5020    2427 
   Size:        556    2037    2875

  Fragments arranged by size: 

                2875    2037     556
 
BlpI GC'TnA_GC

Cuts at:   1796    2580    1796 
   Size:        784    4684
 
Bme1580I G_kGCm'C

Cuts at:    358     690    1481    1742    2043    2275    3093    3186    4242 
   Size:        332     791     261     301     232     818      93    1056

Cuts at:   4242     358 
   Size:       1584

  Fragments arranged by size: 

                1584    1056     818     791     332     301     261     232
                  93
 
BmrI ACTGGGnnnn_n'

Cuts at:    692    2680    2761    3096    5240     692 
   Size:       1988      81     335    2144     920

  Fragments arranged by size: 

                2144    1988     920     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1008    3835    1008 
   Size:       2827    2641
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    275     623     787    1256    1327    1396    1405    1678     275 
   Size:        348     164     469      71      69       9     273    4065

  Fragments arranged by size: 

                4065     469     348     273     164      71      69       9
            
 
Bpu10I CC'TnA_GC

Cuts at:   1316    1557    1626    2183    1316 
   Size:        241      69     557    4601

  Fragments arranged by size: 

                4601     557     241      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1145    1373    2644    3523    4015    4313    1145 
   Size:        228    1271     879     492     298    2300

  Fragments arranged by size: 

                2300    1271     879     492     298     228
 
BsaI GGTCTCn'nnnn_

Cuts at:   1810    5172    1810 
   Size:       3362    2106
 
BsaAI yAC'GTr

Cuts at:   2821    2821 
   Size:       5468
 
BsaBI GATnn'nnATC

Cuts at:      6    2982    5019       6 
   Size:       2976    2037     455

  Fragments arranged by size: 

                2976    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2899    3147    3849    5345    2899 
   Size:        248     702    1496    3022

  Fragments arranged by size: 

                3022    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1460    2657    3178    3856    4130    4277    4688    1460 
   Size:       1197     521     678     274     147     411    2240

  Fragments arranged by size: 

                2240    1197     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    924     954    1325    1355    2307    2337     924 
   Size:         30     371      30     952      30    4055

  Fragments arranged by size: 

                4055     952     371      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    272     275     435    1668    2211     272 
   Size:          3     160    1233     543    3529

  Fragments arranged by size: 

                3529    1233     543     160       3
 
BseYI C'CCAG_C

Cuts at:    275     943    2893    3651    4228    4724     275 
   Size:        668    1950     758     577     496    1019

  Fragments arranged by size: 

                1950    1019     758     668     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    715     715 
   Size:       5468
 
BsiEI CG_ry'CG

Cuts at:    104    1896    3010    3056    4264     104 
   Size:       1792    1114      46    1208    1308

  Fragments arranged by size: 

                1792    1308    1208    1114      46
 
BsiHKAI G_wGCw'C

Cuts at:   1742    1797    2482    3260    3450    4242    1742 
   Size:         55     685     778     190     792    2968

  Fragments arranged by size: 

                2968     792     778     685     190      55
 
BsmI GAATG_Cn'

Cuts at:   1668    1668 
   Size:       5468
 
Bsp1286I G_dGCh'C

Cuts at:    358     690    1481    1742    1797    1957    2043    2275    2482 
   Size:        332     791     261      55     160      86     232     207

Cuts at:   2482    3093    3186    3260    3450    3512    4242     358 
   Size:        611      93      74     190      62     730    1584

  Fragments arranged by size: 

                1584     791     730     611     332     261     232     207
                 190     160      93      86      74      62      55
 
BspEI T'CCGG_A

Cuts at:   1460    4688    1460 
   Size:       3228    2240
 
BspHI T'CATG_A

Cuts at:   2192    4644    2192 
   Size:       2452    3016
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1837    1930    2042    2287    2409    2518    2732    3034    3415 
   Size:         93     112     245     122     109     214     302     381

Cuts at:   3415    1837 
   Size:       3890

  Fragments arranged by size: 

                3890     381     302     245     214     122     112     109
                  93
 
BsrBI CCG'CTC

Cuts at:     50     562    3760    3814    5298    5463      50 
   Size:        512    3198      54    1484     165      55

  Fragments arranged by size: 

                3198    1484     512     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    195     507     779    1243    1619    2381    2460    3380     195 
   Size:        312     272     464     376     762      79     920    2283

  Fragments arranged by size: 

                2283     920     762     464     376     312     272      79
            
 
BsrFI r'CCGG_y

Cuts at:   1891    3466    3647    3856    1891 
   Size:       1575     181     209    3503

  Fragments arranged by size: 

                3503    1575     209     181
 
BssSI C'ACGA_G

Cuts at:   3739    4097    3739 
   Size:        358    5110
 
BstAPI GCAn_nnn'nTGC

Cuts at:    666    2845     666 
   Size:       2179    3289
 
BstBI TT'CG_AA

Cuts at:   3829    3829 
   Size:       5468
 
BstXI CCAn_nnnn'nTGG

Cuts at:    988    1113    1234    1816    2005    2349     988 
   Size:        125     121     582     189     344    4107

  Fragments arranged by size: 

                4107     582     344     189     125     121
 
BstYI r'GATC_y

Cuts at:      1    1962    2427    2493    2960    2983    3318    3564    4576 
   Size:       1961     465      66     467      23     335     246    1012

Cuts at:   4576    4662    4674    5020       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1961    1012     467     465     449     346     335     246
                  86      66      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1008    1008 
   Size:       5468
 
BtgI C'CryG_G

Cuts at:    128    1809    2357     128 
   Size:       1681     548    3239

  Fragments arranged by size: 

                3239    1681     548
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3418    3599    3762    3418 
   Size:        181     163    5124

  Fragments arranged by size: 

                5124     181     163
 
BtsI GCAGTG_nn'

Cuts at:    513    2315    2316    2711     513 
   Size:       1802       1     395    3270

  Fragments arranged by size: 

                3270    1802     395       1
 
DraI TTT'AAA

Cuts at:   2467    2467 
   Size:       5468
 
DraIII CAC_nnn'GTG

Cuts at:    415     415 
   Size:       5468
 
DrdI GACnn_nn'nnGTC

Cuts at:   3174    4032    3174 
   Size:        858    4610
 
EaeI y'GGCC_r

Cuts at:   3053    3227    3618    3645    4990    3053 
   Size:        174     391      27    1345    3531

  Fragments arranged by size: 

                3531    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   3053    3053 
   Size:       5468
 
EarI CTCTTCn'nnn_

Cuts at:    444    3491    3701     444 
   Size:       3047     210    2211

  Fragments arranged by size: 

                3047    2211     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3676    3986    4132    5278    3676 
   Size:        310     146    1146    3866

  Fragments arranged by size: 

                3866    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1795    2480    1795 
   Size:        685    4783
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    275     623     787    1256    1327    1396    1405    1411    1678 
   Size:        348     164     469      71      69       9       6     267

Cuts at:   1678    1709    2312    3293    3725    4472    4740     275 
   Size:         31     603     981     432     747     268    1003

  Fragments arranged by size: 

                1003     981     747     603     469     432     348     268
                 267     164      71      69      31       9       6
 
EcoO109I rG'GnC_Cy

Cuts at:    354     686     977    1338    1347    1477    1901    2607     354 
   Size:        332     291     361       9     130     424     706    3215

  Fragments arranged by size: 

                3215     706     424     361     332     291     130       9
            
 
EcoRI G'AATT_C

Cuts at:   2472    2472 
   Size:       5468
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3487    3487 
   Size:       5468
 
FspI TGC'GCA

Cuts at:   3249    3249 
   Size:       5468
 
HaeII r_GCGC'y

Cuts at:   2190    2902    3150    4172    5151    2190 
   Size:        712     248    1022     979    2507

  Fragments arranged by size: 

                2507    1022     979     712     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    761     793    1143    1175    1325    1357    2194    2226    2419 
   Size:         32     350      32     150      32     837      32     193

Cuts at:   2419    2451    3310    3340    3342    3372     761 
   Size:         32     859      30       2      30    2857

  Fragments arranged by size: 

                2857     859     837     350     193     150      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:    915    2507     915 
   Size:       1592    3876
 
KasI G'GCGC_C

Cuts at:   2898    3146    2898 
   Size:        248    5220
 
KpnI G_GTAC'C

Cuts at:   2488    2488 
   Size:       5468
 
MluI A'CGCG_T

Cuts at:   4901    4901 
   Size:       5468
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1876    4139    4323    1876 
   Size:       2263     184    3021

  Fragments arranged by size: 

                3021    2263     184
 
MscI TGG'CCA

Cuts at:   3229    3229 
   Size:       5468
 
MslI CAynn'nnrTG

Cuts at:   2076    2150    2347    2419    3457    3584    2076 
   Size:         74     197      72    1038     127    3960

  Fragments arranged by size: 

                3960    1038     197     127      74      72
 
MspA1I CmG'CkG

Cuts at:    145     666    1512    1630    2579    2893    3253    4266    4511 
   Size:        521     846     118     949     314     360    1013     245

Cuts at:   4511     145 
   Size:       1102

  Fragments arranged by size: 

                1102    1013     949     846     521     360     314     245
                 118
 
NaeI GCC'GGC

Cuts at:   3649    3649 
   Size:       5468
 
NarI GG'CG_CC

Cuts at:   2899    3147    2899 
   Size:        248    5220
 
NcoI C'CATG_G

Cuts at:    128    1809    2357     128 
   Size:       1681     548    3239

  Fragments arranged by size: 

                3239    1681     548
 
NgoMIV G'CCGG_C

Cuts at:   3647    3647 
   Size:       5468
 
NspI r_CATG'y

Cuts at:   1919    2521    3021    3928    1919 
   Size:        602     500     907    3459

  Fragments arranged by size: 

                3459     907     602     500
 
PasI CC'CwG_GG

Cuts at:    351     351 
   Size:       5468
 
PciI A'CATG_T

Cuts at:   3924    3924 
   Size:       5468
 
PflMI CCAn_nnn'nTGG

Cuts at:    425     828    1444     425 
   Size:        403     616    4449

  Fragments arranged by size: 

                4449     616     403
 
PmeI GTTT'AAAC

Cuts at:   2467    2467 
   Size:       5468
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4607    4639    4607 
   Size:         32    5436
 
PpuMI rG'GwC_Cy

Cuts at:    977    1901     977 
   Size:        924    4544
 
PspOMI G'GGCC_C

Cuts at:    354     686    1477     354 
   Size:        332     791    4345

  Fragments arranged by size: 

                4345     791     332
 
PstI C_TGCA'G

Cuts at:    671     930    1517    2435    2515    3200     671 
   Size:        259     587     918      80     685    2939

  Fragments arranged by size: 

                2939     918     685     587     259      80
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5468
 
PvuII CAG'CTG

Cuts at:    145     666    1512    1630    2893    3253     145 
   Size:        521     846     118    1263     360    2360

  Fragments arranged by size: 

                2360    1263     846     521     360     118
 
RsrII CG'GwC_CG

Cuts at:   3663    3663 
   Size:       5468
 
SacI G_AGCT'C

Cuts at:   1797    2482    1797 
   Size:        685    4783
 
SalI G'TCGA_C

Cuts at:   2505    2505 
   Size:       5468
 
SanDI GG'GwC_CC

Cuts at:    977     977 
   Size:       5468
 
SapI GCTCTTCn'nnn_

Cuts at:   3491    3701    3491 
   Size:        210    5258
 
SbfI CC_TGCA'GG

Cuts at:   2515    2515 
   Size:       5468
 
ScaI AGT'ACT

Cuts at:   4985    4985 
   Size:       5468
 
SexAI A'CCwGG_T

Cuts at:    729    2083     729 
   Size:       1354    4114
 
SfcI C'TryA_G

Cuts at:     33     667     926    1192    1507    1513    1766    2125    2431 
   Size:        634     259     266     315       6     253     359     306

Cuts at:   2431    2511    3196    4189    4380      33 
   Size:         80     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     634     359     315     306     266
                 259     253     191      80       6
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1345    1345 
   Size:       5468
 
SfoI GGC'GCC

Cuts at:   2900    3148    2900 
   Size:        248    5220
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5468
 
SmaI CCC'GGG

Cuts at:   2490    2490 
   Size:       5468
 
SmlI C'TyrA_G

Cuts at:   1160    1388    2623    3538    4030    4292    1160 
   Size:        228    1235     915     492     262    2336

  Fragments arranged by size: 

                2336    1235     915     492     262     228
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5468
 
SphI G_CATG'C

Cuts at:   1919    2521    3021    1919 
   Size:        602     500    4366

  Fragments arranged by size: 

                4366     602     500
 
SspI AAT'ATT

Cuts at:    618     618 
   Size:       5468
 
StuI AGG'CCT

Cuts at:    827    1691    1754     827 
   Size:        864      63    4541

  Fragments arranged by size: 

                4541     864      63
 
StyI C'CwwG_G

Cuts at:    128     822    1580    1809    1998    2357    2602    2956     128 
   Size:        694     758     229     189     359     245     354    2640

  Fragments arranged by size: 

                2640     758     694     359     354     245     229     189
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    342    1632    1757    3051    3184    3643    3852     342 
   Size:       1290     125    1294     133     459     209    1958

  Fragments arranged by size: 

                1958    1294    1290     459     209     133     125
 
TatI w'GTAC_w

Cuts at:    840    4983     840 
   Size:       4143    1325
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1721    1753    2307    2339    4985    5017    1721 
   Size:         32     554      32    2646      32    2172

  Fragments arranged by size: 

                2646    2172     554      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3265    3265 
   Size:       5468
 
XbaI T'CTAG_A

Cuts at:   2499    2499 
   Size:       5468
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    652     876    1110    1183    1816    2963     652 
   Size:        224     234      73     633    1147    3157

  Fragments arranged by size: 

                3157    1147     633     234     224      73
 
XmaI C'CCGG_G

Cuts at:   2488    2488 
   Size:       5468
 
XmnI GAAnn'nnTTC

Cuts at:   2104    2104 
   Size:       5468
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AloI     AscI    AvrII    BfrBI    BmgBI     BmtI
    BsiWI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I     ClaI    CspCI
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PfoI     PmlI    PshAI
     PsiI    PspXI     PsrI    SacII    SgrAI    SnaBI     SrfI     SwaI
     XhoI     ZraI
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