Restriction map for pF1KB8986
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
BamHI       BbeI        BbrPI       BbsI        BcgI        
BciVI       BclI        BcnI        BglII       BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BseAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       FauI        FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KspI        MamI        MfeI        MluI        MroI        
MspA1I      MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        SacII       SalI        
SbfI        ScaI        SexAI       SfcI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        476  GGTACC               G^GTACC
AflII         862  CTTAAG               C^TTAAG
AlwNI         742  CAGACGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       476  GGTACC               G^GTACC
AvaI          634  CCCGAG               C^[CT]CG[GA]G
AvrII         546  CCTAGG               C^CTAGG
BanI          476  GGTACC               G^G[CT][GA]CC
BanII          51  GAGCTC               G[GA]GC[CT]^C
BbvCI         148  GCTGAGG              CCTCAGC(-5/-2)
BfaI          547  CTAG                 C^TAG
BfrI          862  CTTAAG               C^TTAAG
BglI           79  GCCAGTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          546  CCTAGG               C^CTAGG
BsaWI         515  ACCGGA               [AT]^CCGG[AT]
BseRI        1068  CTCCTC               GAGGAG(10/8)
BsgI          523  GTGCAG               GTGCAG(16/14)
BsmAI         856  GAGAC                GTCTC(1/5)
BsmBI         856  GAGACG               CGTCTC(1/5)
BsmI          616  GAATGC               GAATGC(1/-1)
BsoBI         634  CCCGAG               C^[CT]CG[GA]G
BssSI        1131  CACGAG               CACGAG(-5/-1)
BstF5I        738  CATCC                GGATG(2/0)
BstYI         903  GGATCT               [GA]^GATC[CT]
CfoI         1047  GCGC                 GCG^C
CpoI          517  CGGACCG              CG^G[AT]CCG
DrdI          169  GACATCGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           95  CCATGG               C^C[GA][CT]GG
FokI          738  CATCC                GGATG(9/13)
HaeII        1046  GGCGCT               [GA]GCGC^[CT]
HapII         516  CCGG                 C^CGG
HhaI         1047  GCGC                 GCG^C
HinP1I       1047  GCGC                 G^CGC
HpaII         516  CCGG                 C^CGG
KpnI          476  GGTACC               GGTAC^C
MaeI          547  CTAG                 C^TAG
MaeII        1058  ACGT                 A^CGT
MflI          903  GGATCT               [GA]^GATC[CT]
MspI          516  CCGG                 C^CGG
NcoI           95  CCATGG               C^CATGG
PleI          357  GACTC                GAGTC(4/5)
PpuMI         638  AGGTCCT              [GA]G^G[AT]CC[CT]
RsrII         517  CGGACCG              CG^G[AT]CCG
SacI           51  GAGCTC               GAGCT^C
SapI           48  GAAGAGC              GCTCTTC(1/4)
SfaNI        1144  GATGC                GCATC(5/9)
SfiI           78  GGCCAGTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          862  CTTAAG               C^T[CT][GA]AG
StuI          152  AGGCCT               AGG^CCT
XhoII         903  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI          532  GGCGTC               G[GA]^CG[CT]C
              550  GGCGTC              
AfaI          135  GTAC                 GT^AC
              477  GTAC                
AluI           52  AGCT                 AG^CT
              866  AGCT                
AspHI          51  GAGCTC               G[AT]GC[AT]^C
              661  GTGCTC              
AvaII         518  GGACC                G^G[AT]CC
              639  GGTCC               
BalI           77  TGGCCA               TGG^CCA
              482  TGGCCA              
BmyI           51  GAGCTC               G[AGT]GC[ACT]^C
              661  GTGCTC              
BsaHI         532  GGCGTC               G[GA]^CG[CT]C
              550  GGCGTC              
BsiHKAI        51  GAGCTC               G[AT]GC[AT]^C
              661  GTGCTC              
BsmFI         167  GGGAC                GGGAC(10/14)
              184  GGGAC               
Bsp1286I       51  GAGCTC               G[AGT]GC[ACT]^C
              661  GTGCTC              
BsrDI        1148  CATTGC               GCAATG(2/0)
              668  GCAATG              
Cac8I         912  GCTGGC               GC[ACGT]^[ACGT]GC
             1091  GCAGGC              
EarI          820  CTCTTC               CTCTTC(1/4)
               48  GAAGAG              
EcoT22I       974  ATGCAT               ATGCA^T
             1145  ATGCAT              
Hin1I         532  GGCGTC               G[GA]^CG[CT]C
              550  GGCGTC              
Ksp632I       820  CTCTTC               CTCTTC(1/4)
               48  GAAGAG              
MluNI          77  TGGCCA               TGG^CCA
              482  TGGCCA              
MscI           77  TGGCCA               TGG^CCA
              482  TGGCCA              
MseI          785  TTAA                 T^TAA
              863  TTAA                
MwoI           79  GCCAGTGGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              947  GCCATTGTTGC         
NlaIV          85  GGGGCC               GG[ACGT]^[ACGT]CC
              476  GGTACC              
NsiI          974  ATGCAT               ATGCA^T
             1145  ATGCAT              
RsaI          135  GTAC                 GT^AC
              477  GTAC                
Tru9I         785  TTAA                 T^TAA
              863  TTAA                
XcmI          224  CCATTGGCCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1128  CCACACGAGTGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AcsI        AlwI        ApoI        BbvI        
BpmI        BsaJI       BsiYI       BslI        BsrI        
BssKI       BstNI       BstXI       Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      HaeIII      HgaI        HinfI       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MslI        MvaI        NdeII       NlaIII      
Sau3AI      Sau96I      ScrFI       StyI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       
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