Restriction map for pF1KB8963
 (Circular) (Six-base) MAPSORT of: pF1KB8963.seq  Check: 7347  from: 1  to: 4163



pF1KB8963  4163 bp

 With 182 enzymes: SgfI * 

                        August 7, 2008 14:13  ..

AccI GT'mk_AC

Cuts at:   1201    1201 
   Size:       4163
 
Acc65I G'GTAC_C

Cuts at:   1179    1179 
   Size:       4163
 
AclI AA'CG_TT

Cuts at:   4003    4003 
   Size:       4163
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    792    1988    2420    3167    3435     792 
   Size:       1196     432     747     268    1520

  Fragments arranged by size: 

                1520    1196     747     432     268
 
AflIII A'CryG_T

Cuts at:   1515    2619    3596    1515 
   Size:       1104     977    2082

  Fragments arranged by size: 

                2082    1104     977
 
AgeI A'CCGG_T

Cuts at:   2551    2551 
   Size:       4163
 
AleI CACnn'nnGTG

Cuts at:    220     220 
   Size:       4163
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    145     145 
   Size:       4163
 
AlwNI CAG_nnn'CTG

Cuts at:   1468    3035    1468 
   Size:       1567    2596
 
ApaLI G'TGCA_C

Cuts at:    636    2933     636 
   Size:       2297    1866
 
ApoI r'AATT_y

Cuts at:    813    1008    1167    3516    3772     813 
   Size:        195     159    2349     256    1204

  Fragments arranged by size: 

                2349    1204     256     195     159
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4163
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4163
 
AvaI C'yCGr_G

Cuts at:    621    1183     621 
   Size:        562    3601
 
AvrII C'CTAG_G

Cuts at:    916     916 
   Size:       4163
 
BamHI G'GATC_C

Cuts at:    740     920    1188     740 
   Size:        180     268    3715

  Fragments arranged by size: 

                3715     268     180
 
BanI G'GyrC_C

Cuts at:    912    1179    1593    1841    1876     912 
   Size:        267     414     248      35    3199

  Fragments arranged by size: 

                3199     414     267     248      35
 
BanII G_rGCy'C

Cuts at:    864    1177    2207     864 
   Size:        313    1030    2820

  Fragments arranged by size: 

                2820    1030     313
 
BbeI G_GCGC'C

Cuts at:   1597    1845    1597 
   Size:        248    3915
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4129
 
BciVI GTATCCnnnnn_n'

Cuts at:   2061    2822    4163    2061 
   Size:        761    1341    2061

  Fragments arranged by size: 

                2061    1341     761
 
BclI T'GATC_A

Cuts at:    707    1683     707 
   Size:        976    3187
 
BglI GCCn_nnn'nGGC

Cuts at:    180     180 
   Size:       4163
 
BglII A'GATC_T

Cuts at:   1678    3715    1678 
   Size:       2037    2126
 
BlpI GC'TnA_GC

Cuts at:    312    1275     312 
   Size:        963    3200
 
Bme1580I G_kGCm'C

Cuts at:    640    1788    1881    2937     640 
   Size:       1148      93    1056    1866

  Fragments arranged by size: 

                1866    1148    1056      93
 
BmgBI CAC'GTC

Cuts at:   1086    1086 
   Size:       4163
 
BmrI ACTGGGnnnn_n'

Cuts at:    642    1375    1456    1791    3935     642 
   Size:        733      81     335    2144     870

  Fragments arranged by size: 

                2144     870     733     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2530    2530 
   Size:       4163
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    595     736     595 
   Size:        141    4022
 
Bpu10I CC'TnA_GC

Cuts at:    280     433     280 
   Size:        153    4010
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    490    1339    2218    2710    3008     490 
   Size:        849     879     492     298    1645

  Fragments arranged by size: 

                1645     879     849     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3867    3867 
   Size:       4163
 
BsaAI yAC'GTr

Cuts at:    549    1516     549 
   Size:        967    3196
 
BsaBI GATnn'nnATC

Cuts at:      6    1677    3714       6 
   Size:       1671    2037     455

  Fragments arranged by size: 

                2037    1671     455
 
BsaHI Gr'CG_yC

Cuts at:    187    1594    1842    2544    4040     187 
   Size:       1407     248     702    1496     310

  Fragments arranged by size: 

                1496    1407     702     310     248
 
BsaWI w'CCGG_w

Cuts at:   1352    1873    2551    2825    2972    3383    1352 
   Size:        521     678     274     147     411    2132

  Fragments arranged by size: 

                2132     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    544     854     544 
   Size:        310    3853
 
BseYI C'CCAG_C

Cuts at:    143     857    1588    2346    2923    3419     143 
   Size:        714     731     758     577     496     887

  Fragments arranged by size: 

                 887     758     731     714     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    385     620     385 
   Size:        235    3928
 
BsiEI CG_ry'CG

Cuts at:    104     581    1705    1751    2959     104 
   Size:        477    1124      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1124     477      46
 
BsiHKAI G_wGCw'C

Cuts at:    155     554     640    1177    1955    2145    2937     155 
   Size:        399      86     537     778     190     792    1381

  Fragments arranged by size: 

                1381     792     778     537     399     190      86
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1118    1118 
   Size:       4163
 
Bsp1286I G_dGCh'C

Cuts at:    155     554     640     864    1177    1788    1881    1955    2145 
   Size:        399      86     224     313     611      93      74     190

Cuts at:   2145    2207    2937     155 
   Size:         62     730    1381

  Fragments arranged by size: 

                1381     730     611     399     313     224     190      93
                  86      74      62
 
BspEI T'CCGG_A

Cuts at:   3383    3383 
   Size:       4163
 
BspHI T'CATG_A

Cuts at:   3339    3339 
   Size:       4163
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1213    1427    1729    2110    1213 
   Size:        214     302     381    3266

  Fragments arranged by size: 

                3266     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2455    2509    3993    4158      50 
   Size:       2405      54    1484     165      55

  Fragments arranged by size: 

                2405    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2075    2075 
   Size:       4163
 
BsrFI r'CCGG_y

Cuts at:    463    2161    2342    2551     463 
   Size:       1698     181     209    2075

  Fragments arranged by size: 

                2075    1698     209     181
 
BsrGI T'GTAC_A

Cuts at:    521    1048     521 
   Size:        527    3636
 
BssHII G'CGCG_C

Cuts at:    125     167     125 
   Size:         42    4121
 
BssSI C'ACGA_G

Cuts at:    177     633    2434    2792     177 
   Size:        456    1801     358    1548

  Fragments arranged by size: 

                1801    1548     456     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1540    1540 
   Size:       4163
 
BstBI TT'CG_AA

Cuts at:   2524    2524 
   Size:       4163
 
BstXI CCAn_nnnn'nTGG

Cuts at:    225     415     678     831     225 
   Size:        190     263     153    3557

  Fragments arranged by size: 

                3557     263     190     153
 
BstYI r'GATC_y

Cuts at:      1     626     740     920    1188    1655    1678    2013    2259 
   Size:        625     114     180     268     467      23     335     246

Cuts at:   2259    3271    3357    3369    3715       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     625     467     449     346     335     268     246
                 180     114      86      23      12
 
BtgI C'CryG_G

Cuts at:    156     677     887     156 
   Size:        521     210    3432

  Fragments arranged by size: 

                3432     521     210
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    453     657    2113    2294    2457     453 
   Size:        204    1456     181     163    2159

  Fragments arranged by size: 

                2159    1456     204     181     163
 
BtsI GCAGTG_nn'

Cuts at:    402    1406     402 
   Size:       1004    3159
 
DraI TTT'AAA

Cuts at:   1162    1162 
   Size:       4163
 
DrdI GACnn_nn'nnGTC

Cuts at:   1869    2727    1869 
   Size:        858    3305
 
EaeI y'GGCC_r

Cuts at:    335     578     884     941    1088    1748    1922    2313    2340 
   Size:        243     306      57     147     660     174     391      27

Cuts at:   2340    3685     335 
   Size:       1345     813

  Fragments arranged by size: 

                1345     813     660     391     306     243     174     147
                  57      27
 
EagI C'GGCC_G

Cuts at:    578    1748     578 
   Size:       1170    2993
 
EarI CTCTTCn'nnn_

Cuts at:   2186    2396    2186 
   Size:        210    3953
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    180     651    2371    2681    2827    3973     180 
   Size:        471    1720     310     146    1146     370

  Fragments arranged by size: 

                1720    1146     471     370     310     146
 
EcoICRI GAG'CTC

Cuts at:   1175    1175 
   Size:       4163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    595     736     792    1988    2420    3167    3435     595 
   Size:        141      56    1196     432     747     268    1323

  Fragments arranged by size: 

                1323    1196     747     432     268     141      56
 
EcoO109I rG'GnC_Cy

Cuts at:   1302    1302 
   Size:       4163
 
EcoRI G'AATT_C

Cuts at:   1167    1167 
   Size:       4163
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2182    2182 
   Size:       4163
 
FspI TGC'GCA

Cuts at:   1944    1944 
   Size:       4163
 
HaeII r_GCGC'y

Cuts at:   1597    1845    2867    3846    1597 
   Size:        248    1022     979    1914

  Fragments arranged by size: 

                1914    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2005    2035    2037    2067    2005 
   Size:         30       2      30    4101

  Fragments arranged by size: 

                4101      30      30       2
 
HincII GTy'rAC

Cuts at:   1202    1202 
   Size:       4163
 
KasI G'GCGC_C

Cuts at:   1593    1841    1593 
   Size:        248    3915
 
KpnI G_GTAC'C

Cuts at:   1183    1183 
   Size:       4163
 
MluI A'CGCG_T

Cuts at:   3596    3596 
   Size:       4163
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2834    3018    2834 
   Size:        184    3979
 
MscI TGG'CCA

Cuts at:    337     943    1924     337 
   Size:        606     981    2576

  Fragments arranged by size: 

                2576     981     606
 
MslI CAynn'nnrTG

Cuts at:    220     223     301     676     829    2152    2279     220 
   Size:          3      78     375     153    1323     127    2104

  Fragments arranged by size: 

                2104    1323     375     153     127      78       3
 
MspA1I CmG'CkG

Cuts at:    158     971    1274    1588    1948    2961    3206     158 
   Size:        813     303     314     360    1013     245    1115

  Fragments arranged by size: 

                1115    1013     813     360     314     303     245
 
NaeI GCC'GGC

Cuts at:   2344    2344 
   Size:       4163
 
NarI GG'CG_CC

Cuts at:   1594    1842    1594 
   Size:        248    3915
 
NdeI CA'TA_TG

Cuts at:    559     559 
   Size:       4163
 
NgoMIV G'CCGG_C

Cuts at:   2342    2342 
   Size:       4163
 
NspI r_CATG'y

Cuts at:   1216    1716    2623    1216 
   Size:        500     907    2756

  Fragments arranged by size: 

                2756     907     500
 
PciI A'CATG_T

Cuts at:   2619    2619 
   Size:       4163
 
PflMI CCAn_nnn'nTGG

Cuts at:    857     857 
   Size:       4163
 
PmeI GTTT'AAAC

Cuts at:   1162    1162 
   Size:       4163
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3302    3334    3302 
   Size:         32    4131
 
PstI C_TGCA'G

Cuts at:   1210    1895    1210 
   Size:        685    3478
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4163
 
PvuII CAG'CTG

Cuts at:    971    1588    1948     971 
   Size:        617     360    3186

  Fragments arranged by size: 

                3186     617     360
 
RsrII CG'GwC_CG

Cuts at:   2358    2358 
   Size:       4163
 
SacI G_AGCT'C

Cuts at:   1177    1177 
   Size:       4163
 
SacII CC_GC'GG

Cuts at:    159     159 
   Size:       4163
 
SalI G'TCGA_C

Cuts at:   1200    1200 
   Size:       4163
 
SapI GCTCTTCn'nnn_

Cuts at:   2186    2396    2186 
   Size:        210    3953
 
SbfI CC_TGCA'GG

Cuts at:   1210    1210 
   Size:       4163
 
ScaI AGT'ACT

Cuts at:   3680    3680 
   Size:       4163
 
SexAI A'CCwGG_T

Cuts at:    326     326 
   Size:       4163
 
SfcI C'TryA_G

Cuts at:     33    1206    1891    2884    3075      33 
   Size:       1173     685     993     191    1121

  Fragments arranged by size: 

                1173    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   1595    1843    1595 
   Size:        248    3915
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4163
 
SmaI CCC'GGG

Cuts at:   1185    1185 
   Size:       4163
 
SmlI C'TyrA_G

Cuts at:    505    1318    2233    2725    2987     505 
   Size:        813     915     492     262    1681

  Fragments arranged by size: 

                1681     915     813     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4163
 
SphI G_CATG'C

Cuts at:   1216    1716    1216 
   Size:        500    3663
 
StyI C'CwwG_G

Cuts at:    916    1297    1651     916 
   Size:        381     354    3428

  Fragments arranged by size: 

                3428     381     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1746    1879    2338    2547    1746 
   Size:        133     459     209    3362

  Fragments arranged by size: 

                3362     459     209     133
 
TatI w'GTAC_w

Cuts at:    521    1048    3678     521 
   Size:        527    2630    1006

  Fragments arranged by size: 

                2630    1006     527
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3680    3712    3680 
   Size:         32    4131
 
Tth111I GACn'n_nGTC

Cuts at:   1960    1960 
   Size:       4163
 
XbaI T'CTAG_A

Cuts at:   1194    1194 
   Size:       4163
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    678     951    1658     678 
   Size:        273     707    3183

  Fragments arranged by size: 

                3183     707     273
 
XmaI C'CCGG_G

Cuts at:   1183    1183 
   Size:       4163
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NspI     PciI
    PflMI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AloI     ApaI     AscI
     BaeI     BbsI    BbvCI    BfrBI     BmtI    BsaXI    BsiWI     BsmI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SanDI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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