Restriction map for pF1KB8434
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspHI       AspI        AvaII       AvrII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrDI       BsrGI       BssSI       
Bst1107I    BstBI       BstEII      BstF5I      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FokI        FseI        HgaI        Hin1I       
HindIII     HpaI        KasI        Ksp632I     MaeIII      
MamI        MboII       MfeI        MluI        MroI        
MseI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I        389  GGTACC               G^GTACC
AfaI          390  GTAC                 GT^AC
AgeI          246  ACCGGT               A^CCGGT
Asp718I       389  GGTACC               G^GTACC
AvaI          263  CCCGGG               C^[CT]CG[GA]G
BalI           53  TGGCCA               TGG^CCA
BamHI         114  GGATCC               G^GATCC
BanI          389  GGTACC               G^G[CT][GA]CC
BanII         211  GAGCCC               G[GA]GC[CT]^C
BfaI           76  CTAG                 C^TAG
BglI          175  GCCCGCGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          211  GAGCCC               G[AGT]GC[ACT]^C
BsaWI         246  ACCGGT               [AT]^CCGG[AT]
BsgI          295  CTGCAC               GTGCAG(16/14)
BsiEI         244  CGACCG               CG[GA][CT]^CG
BsoBI         263  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      211  GAGCCC               G[AGT]GC[ACT]^C
BspHI         158  TCATGA               T^CATGA
BspMI         392  ACCTGC               ACCTGC(4/8)
BsrBI         364  GAGCGG               CCGCTC(-3/-3)
BsrI           26  CCAGT                ACTGG(1/-1)
BssHII        144  GCGCGC               G^CGCGC
BstAPI        223  GCAGACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         114  GGATCC               [GA]^GATC[CT]
HincII        127  GTCAAC               GT[CT]^[GA]AC
HindII        127  GTCAAC               GT[CT]^[GA]AC
HinfI         125  GAGTC                G^A[ACGT]TC
HphI          239  TCACC                GGTGA(8/7)
KpnI          389  GGTACC               GGTAC^C
KspI           62  CCGCGG               CCGC^GG
MaeI           76  CTAG                 C^TAG
MflI          114  GGATCC               [GA]^GATC[CT]
MluNI          53  TGGCCA               TGG^CCA
MscI           53  TGGCCA               TGG^CCA
MspA1I         62  CCGCGG               C[AC]G^C[GT]G
NaeI          106  GCCGGC               GCC^GGC
NgoMIV        106  GCCGGC               G^CCGGC
NspI           20  GCATGC               [GA]CATG^[CT]
PinAI         246  ACCGGT               A^CCGGT
PleI          125  GAGTC                GAGTC(4/5)
RcaI          158  TCATGA               T^CATGA
RsaI          390  GTAC                 GT^AC
SacII          62  CCGCGG               CCGC^GG
SmaI          263  CCCGGG               CCC^GGG
SpeI           75  ACTAGT               A^CTAGT
SphI           20  GCATGC               GCATG^C
TspRI          27  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         114  GGATCC               [GA]^GATC[CT]
XmaCI         263  CCCGGG               C^CCGGG
XmaI          263  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AluI           95  AGCT                 AG^CT
              188  AGCT                
AlwNI          94  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              224  CAGACCCTG           
AviII         220  TGCGCA               TGC^GCA
              395  TGCGCA              
BseRI           7  GAGGAG               GAGGAG(10/8)
              361  GAGGAG              
BsmFI          72  GGGAC                GGGAC(10/14)
              410  GGGAC               
BsrFI         106  GCCGGC               [GA]^CCGG[CT]
              246  ACCGGT              
Cfr10I        106  GCCGGC               [GA]^CCGG[CT]
              246  ACCGGT              
Cfr13I        302  GGGCC                G^G[ACGT]CC
              369  GGGCC               
DpnII         115  GATC                 ^GATC
              150  GATC                
EaeI           53  TGGCCA               [CT]^GGCC[GA]
              356  TGGCCG              
FspI          220  TGCGCA               TGC^GCA
              395  TGCGCA              
HaeII          58  AGCGCC               [GA]GCGC^[CT]
              172  AGCGCC              
MaeII         320  ACGT                 A^CGT
              380  ACGT                
MboI          115  GATC                 ^GATC
              150  GATC                
MslI          159  CATGATGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              282  CACGCTGGTG          
NdeII         115  GATC                 ^GATC
              150  GATC                
Sau3AI        115  GATC                 ^GATC
              150  GATC                
Sau96I        302  GGGCC                G^G[ACGT]CC
              369  GGGCC               
SfaNI         256  GATGC                GCATC(5/9)
              328  GATGC               


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AccII       AciI        AlwI        BbvI        BcnI        
BsaJI       BsiYI       BslI        BssKI       BstNI       
BstUI       Cac8I       CfoI        DsaI        EcoRII      
FauI        Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       ScrFI       TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com