Restriction map for pF1KA0054
 (Linear) (Six-base) MAPSORT of: KIAA0054.seq  Check: 2303  from: 1  to: 5826



KIAA0054.orf

 With 182 enzymes: SgfI * 

                        August 5, 2008 16:25  ..

AccI GT'mk_AC

Cuts at:      0     240    4494    5826 
   Size:        240    4254    1332

  Fragments arranged by size: 

                4254    1332     240
 
AclI AA'CG_TT

Cuts at:      0    1212    2023    5826 
   Size:       1212     811    3803

  Fragments arranged by size: 

                3803    1212     811
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     584    1574    1756    5333    5344    5551    5826 
   Size:        584     990     182    3577      11     207     275

  Fragments arranged by size: 

                3577     990     584     275     207     182      11
 
AfeI AGC'GCT

Cuts at:      0    5694    5826 
   Size:       5694     132
 
AflII C'TTAA_G

Cuts at:      0      49    5826 
   Size:         49    5777
 
AflIII A'CryG_T

Cuts at:      0     243    1034    2787    5826 
   Size:        243     791    1753    3039

  Fragments arranged by size: 

                3039    1753     791     243
 
AhdI GACnn_n'nnGTC

Cuts at:      0    5757    5826 
   Size:       5757      69
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     312    2573    5826 
   Size:        312    2261    3253

  Fragments arranged by size: 

                3253    2261     312
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2584    2616    5826 
   Size:       2584      32    3210

  Fragments arranged by size: 

                3210    2584      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     811    1458    4339    5775    5826 
   Size:        811     647    2881    1436      51

  Fragments arranged by size: 

                2881    1436     811     647      51
 
ApaI G_GGCC'C

Cuts at:      0    5706    5826 
   Size:       5706     120
 
ApoI r'AATT_y

Cuts at:      0     732    1298    3184    3316    3516    3988    5826 
   Size:        732     566    1886     132     200     472    1838

  Fragments arranged by size: 

                1886    1838     732     566     472     200     132
 
AseI AT'TA_AT

Cuts at:      0    3872    5826 
   Size:       3872    1954
 
AvaI C'yCGr_G

Cuts at:      0    3326    4461    5545    5826 
   Size:       3326    1135    1084     281

  Fragments arranged by size: 

                3326    1135    1084     281
 
AvrII C'CTAG_G

Cuts at:      0     514    5781    5826 
   Size:        514    5267      45

  Fragments arranged by size: 

                5267     514      45
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     944     977    3636    3669    5826 
   Size:        944      33    2659      33    2157

  Fragments arranged by size: 

                2659    2157     944      33      33
 
BamHI G'GATC_C

Cuts at:      0    1914    5458    5826 
   Size:       1914    3544     368

  Fragments arranged by size: 

                3544    1914     368
 
BanI G'GyrC_C

Cuts at:      0     586    3309    3618    5031    5826 
   Size:        586    2723     309    1413     795

  Fragments arranged by size: 

                2723    1413     795     586     309
 
BanII G_rGCy'C

Cuts at:      0    3333    5249    5659    5706    5791    5826 
   Size:       3333    1916     410      47      85      35

  Fragments arranged by size: 

                3333    1916     410      85      47      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      11     169     473    3292    3388    5826 
   Size:         11     158     304    2819      96    2438

  Fragments arranged by size: 

                2819    2438     304     158      96      11
 
BbvCI CC'TCA_GC

Cuts at:      0    4187    4235    4250    4631    5826 
   Size:       4187      48      15     381    1195

  Fragments arranged by size: 

                4187    1195     381      48      15
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     150     184    4546    4580    5826 
   Size:        150      34    4362      34    1246

  Fragments arranged by size: 

                4362    1246     150      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     342    3637    5826 
   Size:        342    3295    2189

  Fragments arranged by size: 

                3295    2189     342
 
BclI T'GATC_A

Cuts at:      0    2463    2871    3414    4758    5826 
   Size:       2463     408     543    1344    1068

  Fragments arranged by size: 

                2463    1344    1068     543     408
 
BfrBI ATG'CAT

Cuts at:      0    1179    5826 
   Size:       1179    4647
 
BglI GCCn_nnn'nGGC

Cuts at:      0     286    5121    5826 
   Size:        286    4835     705

  Fragments arranged by size: 

                4835     705     286
 
BglII A'GATC_T

Cuts at:      0    1161    5826 
   Size:       1161    4665
 
BlpI GC'TnA_GC

Cuts at:      0    5474    5826 
   Size:       5474     352
 
Bme1580I G_kGCm'C

Cuts at:      0     591    5706    5826 
   Size:        591    5115     120

  Fragments arranged by size: 

                5115     591     120
 
BmgBI CAC'GTC

Cuts at:      0    3961    5826 
   Size:       3961    1865
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     585    1404    2209    2319    3255    3572    3974    5826 
   Size:        585     819     805     110     936     317     402    1852

  Fragments arranged by size: 

                1852     936     819     805     585     402     317     110
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4476    4526    5826 
   Size:       4476      50    1300

  Fragments arranged by size: 

                4476    1300      50
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1508    4603    5826 
   Size:       1508    3095    1223

  Fragments arranged by size: 

                3095    1508    1223
 
Bpu10I CC'TnA_GC

Cuts at:      0    2036    4187    4235    4250    4631    5729    5826 
   Size:       2036    2151      48      15     381    1098      97

  Fragments arranged by size: 

                2151    2036    1098     381      97      48      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61    2226    4704    5315    5826 
   Size:         61    2165    2478     611     511

  Fragments arranged by size: 

                2478    2165     611     511      61
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4702    5826 
   Size:       4702    1124
 
BsaAI yAC'GTr

Cuts at:      0     462    1035    2890    2989    5826 
   Size:        462     573    1855      99    2837

  Fragments arranged by size: 

                2837    1855     573     462      99
 
BsaBI GATnn'nnATC

Cuts at:      0     905    5826 
   Size:        905    4921
 
BsaWI w'CCGG_w

Cuts at:      0    3859    4347    5826 
   Size:       3859     488    1479

  Fragments arranged by size: 

                3859    1479     488
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     767     797    2584    2614    2711    2741    3590    3620 
   Size:        767      30    1787      30      97      30     849      30

Cuts at:   3620    4940    4970    5826 
   Size:       1320      30     856

  Fragments arranged by size: 

                1787    1320     856     849     767      97      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1414    2732    3479    4238    4316    5003    5318    5826 
   Size:       1414    1318     747     759      78     687     315     508

  Fragments arranged by size: 

                1414    1318     759     747     687     508     315      78
            
 
BseYI C'CCAG_C

Cuts at:      0     249    2195    4091    4938    5826 
   Size:        249    1946    1896     847     888

  Fragments arranged by size: 

                1946    1896     888     847     249
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      69    4322    5826 
   Size:         69    4253    1504

  Fragments arranged by size: 

                4253    1504      69
 
BsiHKAI G_wGCw'C

Cuts at:      0     791    3123    3166    5659    5791    5826 
   Size:        791    2332      43    2493     132      35

  Fragments arranged by size: 

                2493    2332     791     132      43      35
 
BsmI GAATG_Cn'

Cuts at:      0     878    1726    3345    5826 
   Size:        878     848    1619    2481

  Fragments arranged by size: 

                2481    1619     878     848
 
Bsp1286I G_dGCh'C

Cuts at:      0     591     791    3123    3166    3333    5249    5659    5706 
   Size:        591     200    2332      43     167    1916     410      47

Cuts at:   5706    5791    5826 
   Size:         85      35

  Fragments arranged by size: 

                2332    1916     591     410     200     167      85      47
                  43      35
 
BspEI T'CCGG_A

Cuts at:      0    3859    4347    5826 
   Size:       3859     488    1479

  Fragments arranged by size: 

                3859    1479     488
 
BspHI T'CATG_A

Cuts at:      0    3213    5826 
   Size:       3213    2613
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3594    5826 
   Size:       3594    2232
 
BsrBI CCG'CTC

Cuts at:      0    2600    5826 
   Size:       2600    3226
 
BsrDI GCAATG_nn'

Cuts at:      0     889     926    3152    3701    4400    5110    5460    5808 
   Size:        889      37    2226     549     699     710     350     348

Cuts at:   5808    5826 
   Size:         18

  Fragments arranged by size: 

                2226     889     710     699     549     350     348      37
                  18
 
BsrGI T'GTAC_A

Cuts at:      0    2727    5142    5826 
   Size:       2727    2415     684

  Fragments arranged by size: 

                2727    2415     684
 
BssSI C'ACGA_G

Cuts at:      0    2714    5826 
   Size:       2714    3112
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     937    1501    4057    5826 
   Size:        937     564    2556    1769

  Fragments arranged by size: 

                2556    1769     937     564
 
BstBI TT'CG_AA

Cuts at:      0    2901    3882    5826 
   Size:       2901     981    1944

  Fragments arranged by size: 

                2901    1944     981
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2822    3430    5826 
   Size:       2822     608    2396

  Fragments arranged by size: 

                2822    2396     608
 
BstYI r'GATC_y

Cuts at:      0    1161    1914    3552    3736    5458    5826 
   Size:       1161     753    1638     184    1722     368

  Fragments arranged by size: 

                1722    1638    1161     753     368     184
 
BstZ17I GTA'TAC

Cuts at:      0    4495    5826 
   Size:       4495    1331
 
Bsu36I CC'TnA_GG

Cuts at:      0    5519    5826 
   Size:       5519     307
 
BtgI C'CryG_G

Cuts at:      0     119    1879    2396    3634    5826 
   Size:        119    1760     517    1238    2192

  Fragments arranged by size: 

                2192    1760    1238     517     119
 
BtsI GCAGTG_nn'

Cuts at:      0      81     296    4555    4695    5826 
   Size:         81     215    4259     140    1131

  Fragments arranged by size: 

                4259    1131     215     140      81
 
ClaI AT'CG_AT

Cuts at:      0    1788    5826 
   Size:       1788    4038
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     324     359    1950    1985    5826 
   Size:        324      35    1591      35    3841

  Fragments arranged by size: 

                3841    1591     324      35      35
 
DraI TTT'AAA

Cuts at:      0    5823    5826 
   Size:       5823       3
 
EaeI y'GGCC_r

Cuts at:      0     108     263    1952    2657    2812    4315    5589    5633 
   Size:        108     155    1689     705     155    1503    1274      44

Cuts at:   5633    5826 
   Size:        193

  Fragments arranged by size: 

                1689    1503    1274     705     193     155     155     108
                  44
 
EarI CTCTTCn'nnn_

Cuts at:      0       6    1270    2789    5231    5737    5770    5826 
   Size:          6    1264    1519    2442     506      33      56

  Fragments arranged by size: 

                2442    1519    1264     506      56      33       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     493    5826 
   Size:        493    5333
 
EcoICRI GAG'CTC

Cuts at:      0    5657    5789    5826 
   Size:       5657     132      37

  Fragments arranged by size: 

                5657     132      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     584    1508    1574    1756    4603    5333    5344    5551 
   Size:        584     924      66     182    2847     730      11     207

Cuts at:   5551    5826 
   Size:        275

  Fragments arranged by size: 

                2847     924     730     584     275     207     182      66
                  11
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93     136    4276    5254    5703    5720    5826 
   Size:         93      43    4140     978     449      17     106

  Fragments arranged by size: 

                4140     978     449     106      93      43      17
 
EcoRI G'AATT_C

Cuts at:      0     732    5826 
   Size:        732    5094
 
EcoRV GAT'ATC

Cuts at:      0     457    5826 
   Size:        457    5369
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     662    2302    3017    3058    5826 
   Size:        662    1640     715      41    2768

  Fragments arranged by size: 

                2768    1640     715     662      41
 
HaeII r_GCGC'y

Cuts at:      0    4956    5497    5696    5826 
   Size:       4956     541     199     130

  Fragments arranged by size: 

                4956     541     199     130
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     466     498     834     866    1210    1242    1350    1382 
   Size:        466      32     336      32     344      32     108      32

Cuts at:   1382    1781    1813    3913    3945    4170    4202    4545    4577 
   Size:        399      32    2100      32     225      32     343      32

Cuts at:   4577    5743    5775    5826 
   Size:       1166      32      51

  Fragments arranged by size: 

                2100    1166     466     399     344     343     336     225
                 108      51      32      32      32      32      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1464    1534    3094    5826 
   Size:       1464      70    1560    2732

  Fragments arranged by size: 

                2732    1560    1464      70
 
MfeI C'AATT_G

Cuts at:      0    1447    5826 
   Size:       1447    4379
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     348    1892    2321    5516    5826 
   Size:        348    1544     429    3195     310

  Fragments arranged by size: 

                3195    1544     429     348     310
 
MscI TGG'CCA

Cuts at:      0     110     265    1954    2659    2814    5591    5826 
   Size:        110     155    1689     705     155    2777     235

  Fragments arranged by size: 

                2777    1689     705     235     155     155     110
 
MslI CAynn'nnrTG

Cuts at:      0    1861    2138    4502    5162    5826 
   Size:       1861     277    2364     660     664

  Fragments arranged by size: 

                2364    1861     664     660     277
 
MspA1I CmG'CkG

Cuts at:      0     309    1980    3306    5826 
   Size:        309    1671    1326    2520

  Fragments arranged by size: 

                2520    1671    1326     309
 
NcoI C'CATG_G

Cuts at:      0     119    1879    2396    3634    5826 
   Size:        119    1760     517    1238    2192

  Fragments arranged by size: 

                2192    1760    1238     517     119
 
NdeI CA'TA_TG

Cuts at:      0    2139    2865    3291    5826 
   Size:       2139     726     426    2535

  Fragments arranged by size: 

                2535    2139     726     426
 
NsiI A_TGCA'T

Cuts at:      0    1181    5826 
   Size:       1181    4645
 
NspI r_CATG'y

Cuts at:      0      41     247     306    1183    2472    5167    5826 
   Size:         41     206      59     877    1289    2695     659

  Fragments arranged by size: 

                2695    1289     877     659     206      59      41
 
PasI CC'CwG_GG

Cuts at:      0    1641    4641    5826 
   Size:       1641    3000    1185

  Fragments arranged by size: 

                3000    1641    1185
 
PciI A'CATG_T

Cuts at:      0     243    5826 
   Size:        243    5583
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1880    2397    5826 
   Size:       1880     517    3429

  Fragments arranged by size: 

                3429    1880     517
 
PfoI T'CCnGG_A

Cuts at:      0    5768    5826 
   Size:       5768      58
 
PmlI CAC'GTG

Cuts at:      0     462    5826 
   Size:        462    5364
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1106    1138    2584    2616    5826 
   Size:       1106      32    1446      32    3210

  Fragments arranged by size: 

                3210    1446    1106      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    4276    5254    5826 
   Size:       4276     978     572

  Fragments arranged by size: 

                4276     978     572
 
PshAI GACnn'nnGTC

Cuts at:      0    1588    5826 
   Size:       1588    4238
 
PsiI TTA'TAA

Cuts at:      0    2756    5826 
   Size:       2756    3070
 
PspOMI G'GGCC_C

Cuts at:      0    5702    5826 
   Size:       5702     124
 
PspXI vC'TCGA_Gb

Cuts at:      0    5545    5826 
   Size:       5545     281
 
PstI C_TGCA'G

Cuts at:      0    2060    3344    4335    5582    5826 
   Size:       2060    1284     991    1247     244

  Fragments arranged by size: 

                2060    1284    1247     991     244
 
PvuII CAG'CTG

Cuts at:      0     309    1980    5826 
   Size:        309    1671    3846

  Fragments arranged by size: 

                3846    1671     309
 
SacI G_AGCT'C

Cuts at:      0    5659    5791    5826 
   Size:       5659     132      35

  Fragments arranged by size: 

                5659     132      35
 
SanDI GG'GwC_CC

Cuts at:      0    4276    5826 
   Size:       4276    1550
 
SapI GCTCTTCn'nnn_

Cuts at:      0       6    1270    5826 
   Size:          6    1264    4556

  Fragments arranged by size: 

                4556    1264       6
 
ScaI AGT'ACT

Cuts at:      0     115     951    1121    2230    5826 
   Size:        115     836     170    1109    3596

  Fragments arranged by size: 

                3596    1109     836     170     115
 
SexAI A'CCwGG_T

Cuts at:      0    5256    5826 
   Size:       5256     570
 
SfcI C'TryA_G

Cuts at:      0     183     576    1154    2056    3340    4331    4472    5568 
   Size:        183     393     578     902    1284     991     141    1096

Cuts at:   5568    5578    5826 
   Size:         10     248

  Fragments arranged by size: 

                1284    1096     991     902     578     393     248     183
                 141      10
 
SmaI CCC'GGG

Cuts at:      0    3328    5826 
   Size:       3328    2498
 
SmlI C'TyrA_G

Cuts at:      0      49      76    2241    4719    5330    5545    5826 
   Size:         49      27    2165    2478     611     215     281

  Fragments arranged by size: 

                2478    2165     611     281     215      49      27
 
SnaBI TAC'GTA

Cuts at:      0    2989    5826 
   Size:       2989    2837
 
SpeI A'CTAG_T

Cuts at:      0     436    5826 
   Size:        436    5390
 
SphI G_CATG'C

Cuts at:      0     306    1183    5826 
   Size:        306     877    4643

  Fragments arranged by size: 

                4643     877     306
 
SspI AAT'ATT

Cuts at:      0    1070    5826 
   Size:       1070    4756
 
StyI C'CwwG_G

Cuts at:      0     119     266     514    1879    2396    3383    3634    3756 
   Size:        119     147     248    1365     517     987     251     122

Cuts at:   3756    5781    5826 
   Size:       2025      45

  Fragments arranged by size: 

                2025    1365     987     517     251     248     147     122
                 119      45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2104    2228    2552    3133    3396    3527    3782    5204 
   Size:       2104     124     324     581     263     131     255    1422

Cuts at:   5204    5514    5826 
   Size:        310     312

  Fragments arranged by size: 

                2104    1422     581     324     312     310     263     255
                 131     124
 
TatI w'GTAC_w

Cuts at:      0     113     595     949    1119    1632    2228    2727    3456 
   Size:        113     482     354     170     513     596     499     729

Cuts at:   3456    4827    4870    5142    5826 
   Size:       1371      43     272     684

  Fragments arranged by size: 

                1371     729     684     596     513     499     482     354
                 272     170     113      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     446     478     956     988    1562    1594    1958    1990 
   Size:        446      32     478      32     574      32     364      32

Cuts at:   1990    4835    4867    4940    4972    5826 
   Size:       2845      32      73      32     854

  Fragments arranged by size: 

                2845     854     574     478     446     364      73      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    5258    5826 
   Size:       5258     568
 
XhoI C'TCGA_G

Cuts at:      0    5545    5826 
   Size:       5545     281
 
XmaI C'CCGG_G

Cuts at:      0    3326    5826 
   Size:       3326    2500
 
XmnI GAAnn'nnTTC

Cuts at:      0    2282    4441    5826 
   Size:       2282    2159    1385

  Fragments arranged by size: 

                2282    2159    1385
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AfeI    AflII   AflIII     AhdI     AlfI
     AloI    AlwNI     ApaI     ApoI     AseI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI    HaeII    Hin4I  HindIII     MfeI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PstI    PvuII     SacI    SanDI     SapI     ScaI    SexAI     SfcI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AgeI     AleI    ApaLI     AscI    AsiSI
     BbeI     BmtI    BsaHI    BsiEI    BsiWI    BsmBI    BsrFI   BssHII
   BstEII    BtgZI   DraIII     DrdI     EagI    EcoNI     FseI     FspI
    FspAI   HincII     HpaI     KasI     KpnI     MluI     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     PacI     PmeI     PsrI     PvuI
    RsrII    SacII     SalI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SrfI     StuI     SwaI     XbaI     XcmI     ZraI
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