Restriction map for pF1KF0137
 (Linear) (Six-base) MAPSORT of: FLJ00137.seq  Check: 5439  from: 1  to: 4524



FLJ00137.orf

 With 182 enzymes: SgfI * 

                        August 5, 2008 16:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3594    4524 
   Size:       3594     930
 
AccI GT'mk_AC

Cuts at:      0    2049    3339    4524 
   Size:       2049    1290    1185

  Fragments arranged by size: 

                2049    1290    1185
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     829     954    1861    3085    3486    3989    3998 
   Size:        641     188     125     907    1224     401     503       9

Cuts at:   3998    4145    4453    4524 
   Size:        147     308      71

  Fragments arranged by size: 

                1224     907     641     503     401     308     188     147
                 125      71       9
 
AfeI AGC'GCT

Cuts at:      0    1292    4524 
   Size:       1292    3232
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     302     334    4524 
   Size:        302      32    4190

  Fragments arranged by size: 

                4190     302      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2314    2866    3546    3600    3902    4524 
   Size:       2314     552     680      54     302     622

  Fragments arranged by size: 

                2314     680     622     552     302      54
 
ApaI G_GGCC'C

Cuts at:      0     127    4524 
   Size:        127    4397
 
ApaLI G'TGCA_C

Cuts at:      0    1831    2119    4524 
   Size:       1831     288    2405

  Fragments arranged by size: 

                2405    1831     288
 
ApoI r'AATT_y

Cuts at:      0    1257    3859    3891    4339    4524 
   Size:       1257    2602      32     448     185

  Fragments arranged by size: 

                2602    1257     448     185      32
 
AseI AT'TA_AT

Cuts at:      0    2459    4524 
   Size:       2459    2065
 
AvaI C'yCGr_G

Cuts at:      0      70     126     356    4524 
   Size:         70      56     230    4168

  Fragments arranged by size: 

                4168     230      70      56
 
BamHI G'GATC_C

Cuts at:      0    2079    4524 
   Size:       2079    2445
 
BanI G'GyrC_C

Cuts at:      0    1301    2856    3451    4524 
   Size:       1301    1555     595    1073

  Fragments arranged by size: 

                1555    1301    1073     595
 
BanII G_rGCy'C

Cuts at:      0     127    1892    1945    2021    3312    4076    4466    4524 
   Size:        127    1765      53      76    1291     764     390      58

  Fragments arranged by size: 

                1765    1291     764     390     127      76      58      53
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      99    1417    1849    4506    4524 
   Size:         99    1318     432    2657      18

  Fragments arranged by size: 

                2657    1318     432      99      18
 
BbvCI CC'TCA_GC

Cuts at:      0    3079    4524 
   Size:       3079    1445
 
BglI GCCn_nnn'nGGC

Cuts at:      0      83    4008    4524 
   Size:         83    3925     516

  Fragments arranged by size: 

                3925     516      83
 
BglII A'GATC_T

Cuts at:      0    2790    4524 
   Size:       2790    1734
 
BlpI GC'TnA_GC

Cuts at:      0    1323    3637    4524 
   Size:       1323    2314     887

  Fragments arranged by size: 

                2314    1323     887
 
Bme1580I G_kGCm'C

Cuts at:      0      71     127    1835    2123    3276    3454    4524 
   Size:         71      56    1708     288    1153     178    1070

  Fragments arranged by size: 

                1708    1153    1070     288     178      71      56
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3052    3309    4524 
   Size:       3052     257    1215

  Fragments arranged by size: 

                3052    1215     257
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2549    4524 
   Size:       2549    1975
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     686     780    1856    2372    2405    2610    2791    3391 
   Size:        686      94    1076     516      33     205     181     600

Cuts at:   3391    3412    3861    4402    4524 
   Size:         21     449     541     122

  Fragments arranged by size: 

                1076     686     600     541     516     449     205     181
                 122      94      33      21
 
Bpu10I CC'TnA_GC

Cuts at:      0    3079    3518    4524 
   Size:       3079     439    1006

  Fragments arranged by size: 

                3079    1006     439
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2147    2265    2977    4047    4524 
   Size:       2147     118     712    1070     477

  Fragments arranged by size: 

                2147    1070     712     477     118
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     204     356    2075    3875    4129    4524 
   Size:        204     152    1719    1800     254     395

  Fragments arranged by size: 

                1800    1719     395     254     204     152
 
BsaAI yAC'GTr

Cuts at:      0    1830    4524 
   Size:       1830    2694
 
BsaHI Gr'CG_yC

Cuts at:      0    3849    4524 
   Size:       3849     675
 
BsaWI w'CCGG_w

Cuts at:      0    1895    3695    4215    4524 
   Size:       1895    1800     520     309

  Fragments arranged by size: 

                1895    1800     520     309
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     164     194     302     332    1354    1384    3364    3394 
   Size:        164      30     108      30    1022      30    1980      30

Cuts at:   3394    4524 
   Size:       1130

  Fragments arranged by size: 

                1980    1130    1022     164     108      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     344     833    1478    1520    2150    2362    2596    3414 
   Size:        344     489     645      42     630     212     234     818

Cuts at:   3414    3417    4524 
   Size:          3    1107

  Fragments arranged by size: 

                1107     818     645     630     489     344     234     212
                  42       3
 
BseYI C'CCAG_C

Cuts at:      0     874    3083    4524 
   Size:        874    2209    1441

  Fragments arranged by size: 

                2209    1441     874
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1131    2400    3691    4524 
   Size:       1131    1269    1291     833

  Fragments arranged by size: 

                1291    1269    1131     833
 
BsiHKAI G_wGCw'C

Cuts at:      0    1835    2123    4076    4466    4524 
   Size:       1835     288    1953     390      58

  Fragments arranged by size: 

                1953    1835     390     288      58
 
BsmI GAATG_Cn'

Cuts at:      0     690    4058    4524 
   Size:        690    3368     466

  Fragments arranged by size: 

                3368     690     466
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     945    4524 
   Size:        945    3579
 
Bsp1286I G_dGCh'C

Cuts at:      0      71     127    1835    1892    1945    2021    2123    3276 
   Size:         71      56    1708      57      53      76     102    1153

Cuts at:   3276    3312    3454    4076    4466    4524 
   Size:         36     142     622     390      58

  Fragments arranged by size: 

                1708    1153     622     390     142     102      76      71
                  58      57      56      53      36
 
BspEI T'CCGG_A

Cuts at:      0    3695    4524 
   Size:       3695     829
 
BspHI T'CATG_A

Cuts at:      0     486    1761    1950    4524 
   Size:        486    1275     189    2574

  Fragments arranged by size: 

                2574    1275     486     189
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3594    4524 
   Size:       3594     930
 
BsrDI GCAATG_nn'

Cuts at:      0     934    1668    4524 
   Size:        934     734    2856

  Fragments arranged by size: 

                2856     934     734
 
BsrGI T'GTAC_A

Cuts at:      0    1611    4524 
   Size:       1611    2913
 
BstBI TT'CG_AA

Cuts at:      0     753    4524 
   Size:        753    3771
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3174    3390    4423    4524 
   Size:       3174     216    1033     101

  Fragments arranged by size: 

                3174    1033     216     101
 
BstYI r'GATC_y

Cuts at:      0     933    2079    2572    2790    4524 
   Size:        933    1146     493     218    1734

  Fragments arranged by size: 

                1734    1146     933     493     218
 
Bsu36I CC'TnA_GG

Cuts at:      0    2210    2430    4524 
   Size:       2210     220    2094

  Fragments arranged by size: 

                2210    2094     220
 
BtgI C'CryG_G

Cuts at:      0      13    4524 
   Size:         13    4511
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      74    4524 
   Size:         74    4450
 
BtsI GCAGTG_nn'

Cuts at:      0    3491    3638    3956    4503    4524 
   Size:       3491     147     318     547      21

  Fragments arranged by size: 

                3491     547     318     147      21
 
ClaI AT'CG_AT

Cuts at:      0    2269    4524 
   Size:       2269    2255
 
DraIII CAC_nnn'GTG

Cuts at:      0    2970    4524 
   Size:       2970    1554
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2045    4524 
   Size:       2045    2479
 
EaeI y'GGCC_r

Cuts at:      0    1184    1916    2748    3108    3176    3290    4524 
   Size:       1184     732     832     360      68     114    1234

  Fragments arranged by size: 

                1234    1184     832     732     360     114      68
 
EarI CTCTTCn'nnn_

Cuts at:      0     805     810     973    1167    2009    2518    3487    4524 
   Size:        805       5     163     194     842     509     969    1037

  Fragments arranged by size: 

                1037     969     842     805     509     194     163       5
            
 
EcoICRI GAG'CTC

Cuts at:      0    4074    4464    4524 
   Size:       4074     390      60

  Fragments arranged by size: 

                4074     390      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     686     780     829     954    1856    1861    2372 
   Size:        641      45      94      49     125     902       5     511

Cuts at:   2372    2405    2610    2791    3085    3391    3412    3486    3861 
   Size:         33     205     181     294     306      21      74     375

Cuts at:   3861    3989    3998    4145    4402    4453    4524 
   Size:        128       9     147     257      51      71

  Fragments arranged by size: 

                 902     641     511     375     306     294     257     205
                 181     147     128     125      94      74      71      51
                  49      45      33      21       9       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2162    4524 
   Size:       2162    2362
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75    2311    3054    3460    4412    4524 
   Size:         75    2236     743     406     952     112

  Fragments arranged by size: 

                2236     952     743     406     112      75
 
EcoRI G'AATT_C

Cuts at:      0    3859    4524 
   Size:       3859     665
 
EcoRV GAT'ATC

Cuts at:      0     335    4524 
   Size:        335    4189
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     868    2259    2602    3301    3516    4092    4483    4524 
   Size:        868    1391     343     699     215     576     391      41

  Fragments arranged by size: 

                1391     868     699     576     391     343     215      41
            
 
HaeII r_GCGC'y

Cuts at:      0     852    1294    3679    4524 
   Size:        852     442    2385     845

  Fragments arranged by size: 

                2385     852     845     442
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     619     651    1162    1194    1354    1386    1932    1964 
   Size:        619      32     511      32     160      32     546      32

Cuts at:   1964    2557    2589    3181    3213    3232    3264    3364    3396 
   Size:        593      32     592      32      19      32     100      32

Cuts at:   3396    3781    3813    4524 
   Size:        385      32     711

  Fragments arranged by size: 

                 711     619     593     592     546     511     385     160
                 100      32      32      32      32      32      32      32
                  32      32      19
 
HincII GTy'rAC

Cuts at:      0     860    2050    4174    4301    4524 
   Size:        860    1190    2124     127     223

  Fragments arranged by size: 

                2124    1190     860     223     127
 
HindIII A'AGCT_T

Cuts at:      0    1540    1735    2897    4524 
   Size:       1540     195    1162    1627

  Fragments arranged by size: 

                1627    1540    1162     195
 
HpaI GTT'AAC

Cuts at:      0    4301    4524 
   Size:       4301     223
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     281    1397    1609    1746    1835    4179    4524 
   Size:        281    1116     212     137      89    2344     345

  Fragments arranged by size: 

                2344    1116     345     281     212     137      89
 
MscI TGG'CCA

Cuts at:      0    1186    2750    3110    3178    3292    4524 
   Size:       1186    1564     360      68     114    1232

  Fragments arranged by size: 

                1564    1232    1186     360     114      68
 
MslI CAynn'nnrTG

Cuts at:      0     526     584    1766    1828    1952    2137    3724    4524 
   Size:        526      58    1182      62     124     185    1587     800

  Fragments arranged by size: 

                1587    1182     800     526     185     124      62      58
            
 
MspA1I CmG'CkG

Cuts at:      0      15      53     894    1050    1115    1499    2392    2866 
   Size:         15      38     841     156      65     384     893     474

Cuts at:   2866    2913    3083    3327    4524 
   Size:         47     170     244    1197

  Fragments arranged by size: 

                1197     893     841     474     384     244     170     156
                  65      47      38      15
 
NspI r_CATG'y

Cuts at:      0     316    4093    4524 
   Size:        316    3777     431

  Fragments arranged by size: 

                3777     431     316
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     605    1754    2866    3299    3648    3902    4524 
   Size:        605    1149    1112     433     349     254     622

  Fragments arranged by size: 

                1149    1112     622     605     433     349     254
 
PfoI T'CCnGG_A

Cuts at:      0    2009    4524 
   Size:       2009    2515
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     302     334    4524 
   Size:        302      32    4190

  Fragments arranged by size: 

                4190     302      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2311    3054    4412    4524 
   Size:       2311     743    1358     112

  Fragments arranged by size: 

                2311    1358     743     112
 
PshAI GACnn'nnGTC

Cuts at:      0     103    4524 
   Size:        103    4421
 
PspOMI G'GGCC_C

Cuts at:      0     123    4524 
   Size:        123    4401
 
PspXI vC'TCGA_Gb

Cuts at:      0     356    4524 
   Size:        356    4168
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3544    3576    4524 
   Size:       3544      32     948

  Fragments arranged by size: 

                3544     948      32
 
PstI C_TGCA'G

Cuts at:      0      58    2817    3216    3326    3486    3551    4246    4524 
   Size:         58    2759     399     110     160      65     695     278

  Fragments arranged by size: 

                2759     695     399     278     160     110      65      58
            
 
PvuII CAG'CTG

Cuts at:      0     894    1050    1115    1499    2866    2913    3083    3327 
   Size:        894     156      65     384    1367      47     170     244

Cuts at:   3327    4524 
   Size:       1197

  Fragments arranged by size: 

                1367    1197     894     384     244     170     156      65
                  47
 
SacI G_AGCT'C

Cuts at:      0    4076    4466    4524 
   Size:       4076     390      58

  Fragments arranged by size: 

                4076     390      58
 
SacII CC_GC'GG

Cuts at:      0      16    4524 
   Size:         16    4508
 
SalI G'TCGA_C

Cuts at:      0    2048    4524 
   Size:       2048    2476
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2009    3487    4524 
   Size:       2009    1478    1037

  Fragments arranged by size: 

                2009    1478    1037
 
ScaI AGT'ACT

Cuts at:      0    4062    4524 
   Size:       4062     462
 
SfcI C'TryA_G

Cuts at:      0      54     689    2813    3119    3212    3322    3340    3482 
   Size:         54     635    2124     306      93     110      18     142

Cuts at:   3482    3547    4242    4524 
   Size:         65     695     282

  Fragments arranged by size: 

                2124     695     635     306     282     142     110      93
                  65      54      18
 
SmaI CCC'GGG

Cuts at:      0      72     128    4524 
   Size:         72      56    4396

  Fragments arranged by size: 

                4396      72      56
 
SmlI C'TyrA_G

Cuts at:      0     356    2162    2244    2992    4026    4524 
   Size:        356    1806      82     748    1034     498

  Fragments arranged by size: 

                1806    1034     748     498     356      82
 
SphI G_CATG'C

Cuts at:      0    4093    4524 
   Size:       4093     431
 
SrfI GCCC'GGGC

Cuts at:      0     128    4524 
   Size:        128    4396
 
SspI AAT'ATT

Cuts at:      0    1685    4524 
   Size:       1685    2839
 
StuI AGG'CCT

Cuts at:      0    3832    4011    4360    4524 
   Size:       3832     179     349     164

  Fragments arranged by size: 

                3832     349     179     164
 
StyI C'CwwG_G

Cuts at:      0    1103    1131    1280    1310    3032    4139    4524 
   Size:       1103      28     149      30    1722    1107     385

  Fragments arranged by size: 

                1722    1107    1103     385     149      30      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1319    3291    4524 
   Size:       1319    1972    1233

  Fragments arranged by size: 

                1972    1319    1233
 
TatI w'GTAC_w

Cuts at:      0     233    1611    1714    2554    3737    4060    4524 
   Size:        233    1378     103     840    1183     323     464

  Fragments arranged by size: 

                1378    1183     840     464     323     233     103
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     164     196    1530    1562    2941    2973    4524 
   Size:        164      32    1334      32    1379      32    1551

  Fragments arranged by size: 

                1551    1379    1334     164      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3058    4524 
   Size:       3058    1466
 
XbaI T'CTAG_A

Cuts at:      0     769    2398    4524 
   Size:        769    1629    2126

  Fragments arranged by size: 

                2126    1629     769
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3300    4524 
   Size:       3300    1224
 
XhoI C'TCGA_G

Cuts at:      0     356    4524 
   Size:        356    4168
 
XmaI C'CCGG_G

Cuts at:      0      70     126    4524 
   Size:         70      56    4398

  Fragments arranged by size: 

                4398      70      56
 
XmnI GAAnn'nnTTC

Cuts at:      0     560    1685    2669    3536    4524 
   Size:        560    1125     984     867     988

  Fragments arranged by size: 

                1125     988     984     867     560
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrGI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII
  HindIII     HpaI     MmeI     MscI     MslI   MspA1I     NspI    PflMI
     PfoI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
    PvuII     SacI    SacII     SalI     SapI     ScaI     SfcI     SmaI
     SmlI     SphI     SrfI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AscI    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI
     BclI    BfrBI    BmgBI     BmtI    BsaBI    BsiEI    BsiWI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI   BstEII  BstZ17I    CspCI     DraI
     EagI     EciI     FseI     FspI    FspAI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PsiI
     PvuI    RsrII    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SwaI     ZraI
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